FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\AT02321
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General Information
Symbol
Dmel\AT02321
Species
D. melanogaster
Name
AT02321
FlyBase ID
FBcl0016807
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG4744
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Stapleton et al., 2002)
      testis
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      4,692
      NCBI
      BT001259
      Full Length sequence dataSequence Downloader
      TTCACATTTGACTTATTCTTTACAAAACTATAACTGACAGCATGATTTTCCTACAACTCTGGTGGGCGCTGTTGGTGTTC
AGTAGTCCTGGCTCCGCCCAGCAGATCTCCGTCGAGGAGGAGAAACACCCGGTTGAGGGCAGCGATAAGTTGCTCAGCTT
CAGCCGGGCAGTGGACCTGGTACGTCCCGAGTTGCAGGAGCTTAAGGCTTCCTACAGCGGTCGCCAGGAGCAGGATGCTC
TCAAGCAGCGGGATCGACGGAACTCAATCGACAATATGCTGGCCAGTTTTGCCCAAGGGGATGTTGGGAAATCGAAGGAT
GAATCTGATGACGAAGATCGAATGCTCCAGGAGGCGGCGGACGCTTGGTTCCGTGACATTCAGGCCAGCATGTCGAAGAG
CAATCCCTATAAGAAGCGATCTGGGGACAAACAGCAGAGATCCATAGGCAAGAATATTATCGACCAGCATGCCAAGGCCC
TGGAGCGCACCGAGGTCGGGAAGGTTAAGTTGAAGTACAAGCAAATGGGCGATGACACCATTGTTCGCAAGTTCCAGGTG
ACTAAGTACCCTGAGATTACGCATAACACTGGGATGACCCACCTAGACGTAAAGCTGAAGCAGGTGCAGCAGATGCTGAC
CGACCAGGAGAGTCTGGAGGCCAGGCAGGTGGCGAAGAAGACTGAGAGGCAGGAAAAGGTTAAGGAATCAAAATGGGTGG
ACAAGTTGCCCGAGTTCGGTGATAGCCCCATTCCGGATGGGATCTACGAGAGTACACTAAATCGCCTGGCCTTTCCCTAT
GCCACGTCGCACAAGCTGGACCACGAGGCCAACTACAAGCTCTCCAAGGTCGAGCAGGAGGCGTCCGAGAAGGGCTCCAG
CAAGCACTCCAAACCGGGCAGGATTAGCGAAGGTCTTGTGAAGCAGCTCAAGCGGAGCTCGTGCAGTGGCTTTAATCCCA
TGAACGAGATCAAACTGAAGACCAGCAGTCGACTGATGCTCAAGGGTCTAGGTCCATCGCTTCCAAGTAACGCTCTGCTG
GACATCCATCGTAACCAAATGGTGGACAGCATGATCCGGCGGAGGGAGCTGGAGGTACAACAGCAGCGGCACCAGCAGCA
TCAGCAGCAACTGGAGTCTAGCGAGGATATCGACGATATGGATCAGCGAATGGGAATTAATAACTTAATGGCACAGAGTG
AGAACTTGGAGTACCTACAGCCCATCAATACCGGCATGGATGGGAATCGAATGATGGAGCTGCCACAGAGCTACGACTAC
CGAGTGCATCAGTACGATCGTTTTCATCCTCTGAACCAAAGGCAAATGTCTAATGTGGGCGATTACGAGAGGAGTAATCG
CATGCCGCTCGACCTGGATCGTTTCAAAAGGGAACCGCATTCGGAGTCGTCTTTTCTGGAAGCCCCTAATGAATTAAGTT
TAGGCCAATTATCTGTAGAGGAACCGGTCCTTAAGTCCGAGGATGAAAATATATCCGATCATGAGATGGCCATGAAAATT
GCAGTATTGGACATTAAGGATGACAAAGCCTTACATTCAACCGGCGATGGGGAATCCAAAGAAAAGGACACACTTGAAGA
GGTTAAATTAGAGGGCCTACATGAGGAGCTAAAGTTGGATGAGGAATCTTTAGTTAAACCCGAAAGTAAACTTGATTCGG
AGGTATCGTGCATCAATGGAAAATTTGAGGATAGATCAGAGATGGAAGTGGAAAACAAACCATTGGGACCGGTAAAACGT
GAAGCCAAGGCAGAGCTAATCAACCAAATGGGCAATAAGCTGAAGAATGATGGATCCACTTTGGTAGCTAAATCAAGTGC
AGAGTCGCCTTTAAAAGAAGATGTACCACCGAATTTGGCCAGAAGAAGCGAACTCAATGTAAGTACTGGTACAGATTTGT
CCGCCGTCTCCAAAAAGCAAAACAGTTTAAAGAAACGTAAAAGTTCTGCAACGTCAGATGATTCCGATAAGCATGACAGT
AATCCCGATTGTGACACGGAGTGCAAGTCGGAGGATGCGGAAAAGTCGGAAACGGATGCAGAGAAGGACGACAGCATTGT
CAAAGTCAACATCAAGCTTAAGCTTCAGGAACCGGTCGAAGAGAAATCAGAATGCGAGGAAATCAGTAAAGTGGTGGGCA
AAGGGGCTGCTGCATCGGCTCCGAAAGTGAATCCCGATGCTGTGGTGAAACTGTTGGCAAATGAATTGTCCATGGACAAG
GACCGTGGAAAGCGGCAAGCAAGGCATCGAAGATATCGTAGACAGAACAGGAGATCCAAGAGATCGCTGGAGGATAACGA
TCAGCACGAATCAGATGATAAGCAACATGCTGTGTCCAAACGTTTGGCTCCTCTGCTGGATGACTTCGAAGACCACAATG
GCAATCGTGTTGGTTTCGGGAATCTGGGTAATGGTATGCTCACACCGGATGCAGAGTATTCGGGTGAAAGGTCATATCCC
AGCCATGAAATGCTGGCTCACTTTGATGAACTGGAAACTGCTCATGACGGCGATTCCATGGTACCGATCTACGATCCCAC
TAAGGTCGTGGTACCCATGGTGAATCAGCGACCGAATGGCGGCTTTCAGGGGCAGCAGCCTGTGCAGATGCAATTTTCAC
AGCAGCAGCCGCAGCAGCAGATGCAGCAGCAGATGCAGCAACAGCATATGCAGCAGCAACCACCGCAGCAGCCACAACAG
CAGCAACAGCAGCAACAGCAGCAACAGCAGCAACAACAGCAACAAGTGCAACAGCCGCAACAACACGATCTAAGCCGTCA
GCGTCATCAATCAAGTAATTTTCAGGCAAGTTTTAATCTAACCCATTTGTTCAATGAGTTAGAAAAGTTGCAAAATATGA
AGACACCACAGCCACAACAACAGCAACAGAAGTTGCAACAGCAATTGATGCCACAACAGCCACTAATGCCACAACAATTC
CAGTTGCCGCAACAGCAAATGCCGGTGCAACGAAATATTCAACAATTGCCCGAAAAGCCCATTTCGCCACCTATTAAATC
CTTTGGAGCCCATCGTTACTTTGGACCCTTCTTCAACAAGCAGGTCAAACACGACCCTTACATGGCGGCGATAGGAACCT
TGACTACTTTGGATCCCAGATGTGTTGCCATTACGGCTACTGCCGCCACTCCAGTTGGAAATATAACTAACGCCTTTACT
TTGCCTGCCAATTGCACAACTACTACGGTTCCCAACAGTCCAGTGTCATCCACACCAGCCCCATGTACCAATCAAACGAG
TCAAACGGATTTGGCTTGCCGAGCTCCGGATGCGATGAAACTCAACGTTTCCATAAATGCCAATTTGTGCGGAGATCCTA
AAACCAAACAGTTCTATGGCAATGGCTCCATAAGTCTGTTGCCCGGTTATAAGGCGTTGCATTCTGAATTGAATCTCGGC
TATCAGAATGATGAACTTCATGGAAATGCTGCGCAATTGGAGAACGATCTAAGCGATCTGAAGATCAAGGACGAAAGGCG
TATACGCGATGTGGGCGAAGAGTCCAGAGATGAGTGGGAACCAAAAACTGGAAATGAGAAGCCAAGAAAGCAGGAGGGTC
AGATAAGAAGGCCAAGTATTAAGTGGAATAGAAAGTTTAAAAATCGCCAAAAAGCTGCGAAGGCAAAGTTAAAAGAACGT
CCCAATGATGTCTCCAAAGAAGCCACACAAGGAGGTACAAAAGAAGCATCAAATGTGGCTACAAATGAAGGACAAAAATC
GTCGGGAAAGTGTCACCGACCTCGGATTGAAATACCGGATTCCCGATCGGAAAGTTACGATAAGCTAATCACGGGCAAAA
TCTCCGAGGACATAGTTTCCGCTGTCTTTGAGGCAGTGGGAAATGATCCGGGCATGGACCGCTTACTGGCGGTTCTGGAG
AGGAATCGCAAGTGTGAACAGAAGCAGTCCAAAAACTTCTATCAAATCCGCAACGACAAGACCGAGAGTTATCTACATCA
CACGGAGACCATGTTGCGTGACACAATGGAGGCGATTAGCAGCATAATTGATGAGCAGGTGCGACGAAGAGCATGCATTC
CTTTGCGTCCGGATCTCCAGGACTTCTATGACCTAATTCTCAAGACATCCAACGAACAGCAAAAGTGTCGCGAGAAACGA
CAGAGCACACTTCAAATCCTTGCCGAGGATTTCAGTCAGGATGTGCGATTGCTCGATCCGAGTAAGATCAGCCAAAAGTC
GAGGATTGTCAAGAAGTTACTGCGACAGTACGAGGATCTACCGATGGAGGATCAACAGGCGGCGGTTGGAGTGCGTGATG
AATTGCTCCTGGATCTGGTTTATCTCCAGAAGATGGCCGAATCCGCAGAGCGCAATCAACGTAACGCCCGTTTGCAGGAG
GTGCTCAAACAGACCAGCCTGGACGAGGTCCTGGAAAAGAGTCTAACCACCGAGTACTCACCGCGTTTTATAAAACTGCT
AAAGACGGCGGAGCTTTATAAGGAGGCGGGCGAGCAGCAGGCCAAGGCATTTGTGGGTCTTTAGACCGCTTTAATCAACG
TTTTAGATTTTTGTTTCAATTCATTAGTTGTTTTTTCCAATTAAAAATTCCCTTTGTTTGAGTTAAGTCATATATTTTTA
ACACATTTGGAATAAGCAACAAAATTCGTTTGAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)