FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\AT17875
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General Information
Symbol
Dmel\AT17875
Species
D. melanogaster
Name
AT17875
FlyBase ID
FBcl0020647
Feature type
cDNA_clone
Computed gene(s)
Dmel\Ccdc39
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI16519
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage | male
    seminal vesicle
    (Stapleton et al., 2002)
    testis
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,128
    NCBI
    BT032643
    Full Length sequence dataSequence Downloader
    TTTAGTTCGGCCTAGCTGAGAGGCTAATCTCTAACACGATGAATCGCACCGACGACATGAAGAATGCCTCGCTGAACGAC
TACAAGCTGGAAGCGATGGCCTCCGTCGGCTGGGCGGTGGACTCGGACATTCCAATGGCGAGTGCCGAGAACTTGGCCAT
CCTAAAGGAACTGACTGCTCTGCGGGCGTTGAAGCTGGAGCTGCAGCAGCACATGAACTCGCTGGATGAGCGCGAGATGG
CGGTGGAACGACATACCCGCAACATTGAGGGCACTATTCAGCAGAACCTGACACTGTACAGCTCCCTGAAGGATGATGTT
ATCAAGGAGGCGCACAACGTCCAGCTTGTGATCTTGGAGCGCAACAAGATCAAGGAGGATCTGCGGAAGAATAACAAGGA
GCTGGAGGAGTACACTGAATATGTCAAAGTCACTGAGCGAAAGATTTGCCAGAGCAAGCGCGAGATAGATGAGCTGACTT
CCCGCATCAAGTCGGCTAAGACTACCTTGGTGGAGTGGACAGAGGCCATGGAAGACGGAAACAAGGGCTACCAGCTAATC
GAAAAGTACTACCTCGATGACCAGCAAAAGGCACGGGAGCTGAACATTAAGCGTCAGCTCTTGCAGGCGGACATCGACAA
GCGGCGCAAGCAGGTGGTGCTCCTCTATGACGAGCAAATGACGCTGGAGAAGAATCTGGAGCGAACCGCTTGTCTGTACA
GGTCTGCCCACGCGGAACGCCGCCAGATGGTGGAGACGTGGAAGAGCGCCGTGAACCAGATGACCCAGCGAGAGCACGAC
ATTCAGCGCAGCGAGATAGAGTGTGCGGAGCTGGCCCAGAAAGCCCAGCAGACGGCTCAGACCTACAAGGAGTACGACAA
CCAGCTAACCGAGGTCATCGAGAACAACCGCCAGGTGGAGCTCGCGATCGAGTCCCTCAACGAGGAAAGCTCCGACATGA
AAAACCAAATCCAGATCCTGATTGATGCCACATTACTGAAGGAGCGCGAGATCGACGGACTGCGTCGGGAACTGGAGAAC
CTTTCGAACCGGGTTCACCTGCAGCGCATGGATAACCGTAGTCAGATGAAAAAGCGCGATGAGAAGGCCAAGGAGCTGGA
GAACTTCGCTTCGGTGATGGAGAAGGTTAACGCTCGGCTGAAGTCTGTACAGAACAAGGCCCTCAATGCGGAGCAGCGGC
TGCAAATTCTGGAGGAGATGATGCAGGCGGAGGAGACGGCTCTGCGAAACCTGGACAAGGAGCAGGAGAAGGTCAACGAG
ATGTTGTACCGCACCCAGAGGCAGGTCATCGAGCTGCAGGACGAGGAGAAGGTCTTGAAGGTGCAGAACGACTCGCTCAA
CTCCAACCTGGCAGCGATCAATCGCAATCAGCAGCAGGTCAATAACGAACTCAAGCGGCAGACGGAGATCCATTACAGCC
TGTCCTTTAAGTGCTTGGAGGCGGAGCGGCGTCTCGCCGAGATCAAGGGTCTGGCAGATGACCCCGAGGTAGAGGCCACA
AACATGGCCCGGCTTAACACTCTGGAGCAGGAGTATGAAAAGTTGCAACGCCTCATCGCCACCACGGAGGCGCAGAATAA
GAAGCTAAACTATAACATGAACAACCTGGTCGTCCAGTACAACGCCGACGAGAAGGAGCTCGAAATGGTCAGGTTCAAGA
TTAAAGAGGCCCAGGTGTACTGCGAGGGCACCGTCAAGAGACTGCGCCAGATTCGATACGAGAATTCCGAGCTCATAGTC
GACCTCAACATGGTCAAGATGCGCTGCAGCGACCTAGAGGTTGGCATCGGAGGCTGCGAGCAGGGCACCTACGACCTAGA
GCAGCACCGCCTTGCCTTCCGGCGCGCCATCAAAGATCGCACCGTTGAGCTTCGCAGCCAAGAGGATGTGCTCCTCCTCA
AGAAGAAGCATCTTAACGAGGAACTAAGCACCCTGCGAGCCGACCTCGGGGAGCGGAAGAAGCAGATTGAGGCGATGAAG
GCGCGCTTTGAACTTACCGCCCAATTGCTAGGCAAAAACGAGGACGGCTCCATCATGACCAGCACACAGCTGAAGGTGGT
GAGCGCCCAGGAACGGCAGATGCTGGCCGACGAGGGCGATGCCCTCAACAAAAAGGTTCTCAAGGCTGAGAAGGAAGTGG
TCGCCCTGGAAAACACACTGCGCCAGTTCGACAAATCGAACGATAACTACCGAAAAACATTCCGATCTGTGGACGAAAAC
TCAAAGGATCGCGAGCGGGCCGAACTGGAGCTGAAGGAACTTGAGGCGGCCTACTGCCGCGAGCTGGAAAAACTGAAGGT
TCTCAGGTGCAAGGCACAGCACTACCAGCAGAAGCACGCAGCCCAGCGAGCTGAGGAGGAAGACCTGATTTCCAAAATAG
AGAAGGCGAAGGCTAGTCGGGCGGAGCACTCTGCAGTTCTGGAGAAGATCGAGCGCGAACTAGATGACCAGCGAATGAAG
CTGGACAGGGCCAATCGAGAGATCCGGACACAGTTGCGGGAGATCAAGGCGCGGCCCTTTAGCGAGGAGTACCTGGCGCA
GTTCGAGCGGGATCTGTCTCTACAGGAGCTGGAGGCGCGTAACACCAAGGCGCTGAACATGATCACCGATCTGGCGAACA
GCGACGAGAGCGGCACGGATATAATTGGAATCCTCTTGCGAAAAGGAATCAAGTTGCCGATGCACCTGAAGCGCACGTGC
AGCCGCGTGTCCTGGAACAGCAGCTCCTCGGGCAAGTCCTCGCAGGGTCAGGACACCGCCTCCTACCTCAATGTTAAGGG
CAAGAAGTTCTTCTGCGACGGGGCTAGTGCCCGGTCCTCCGTCTCCGACATGAGCTCCCTTAAGGATGACACATCCTCCA
CAACCTCCCACTCCGGACTGAGCATCATATCGCTTGAGTTGCCCTTGCCGAAGAAAAAATAATAGTCTAAAATATATTTA
TTTATTATTCCGTTCGATATCCGATGATCTCAACCCGTATGCTCGTATCTTCACGTGTTGGCATCGCAGTGTTTACAAAT
TGACCATCTAAACGAAACAGCGAATAAAATGCGTTGCCCAAGCGGGTCAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
    Library Information (1)
    Library: AT_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

    (Stapleton et al., 2002)
    Sample preparation

    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

    Protocol

    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    AT
    (Stapleton et al., 2002)
    AT_cDNA
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)