FB2026_03 , released September 17, 2026
Clone: Dmel\AT07754
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General Information
Symbol
Dmel\AT07754
Species
D. melanogaster
Name
AT07754
FlyBase ID
FBcl0025679
Feature type
cDNA_clone
Computed gene(s)
Dmel\Psa
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI19310
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage | male
    testis
    (Stapleton et al., 2002)
    seminal vesicle
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,760
    NCBI
    BT125968
    Full Length sequence dataSequence Downloader
    CTGCTAGTGTTTTTATTTTCATTTGCAACTTTTTCGGTCCAAAGTCATGTACAAAAATGCACTGAAAGGCGGTATTCGCA
CGGCCAAGCAGGCTTGGAAGTTAAAAAACGAACTGGGCAAACTCAACTTGGACTTGGCCAAGGCAAACTCAGATTTTGAG
CAGCATTTGTGCGAAGCTGCCAGATATACTCAGAGTTTCCACTTCCTCGCCAGAAACACCCCGAGAACACCTTTGTTGCT
ACTAAAAACGACAGGCGATAACAACAGCTCCCCCCGAATCGTTGCTAACTATTATCGGACAGCTCCAGTTAACCCCCCGA
AGCAGCAGAATCCGAAACCCAGCGGAGCGGCAGATAAGACCACCTCAGTAAAGCATCTTTTTCGAGCAGAAAGTGGCCAC
CTCAACGCGCTAATCTCGTCGAAGCAATTTGTGAAGGTGCAAAAGCAGTTCTACAGGGACTTCTCTTCCGTTTCGCTTGG
AGCCCCTCTGAAGAGCAGTGACGCTCCGTTGCTGGGAGATTTGTGCAGTCAGTATCGCGCCATCCTCGCCCACACATCAG
CGGACGTGATTCCAGGCCAGGATCGCCATCATCCGGGTTGCAACTATCAGCAGATCAGGCGGGCACACACACAGTTACTG
AGCAAGGAGCAGCAGCTGGAGCAGGAGCGCAAGATGGCCCAGAAGGCAAAGTTCGAGAGGCTGCCCACCAATGTGGTGCC
AAGGCACTACGAGCTCCTCCTGCAGCCCAATCTCAAGGAGTTCACCTTCACGGGCAAGACCGTGGTGCAAGTCAATGTCA
AGGAGCCCACTACCCAGATAACCCTGAATGCCCTGGACATTGTTCTCGATTCGGTAGAGCTCCATTTTGAGTGCAGCAAA
CTGAAGCCGGAGAAGATAGTGTACTCTGCGGAAAATGAGACGGCCACGCTGGAGTTTGCCCAAGAGATTCCGGCGGAGAC
TCAAGGCGTACTGCATATGTCCTTTACGGGCGAGTTGAACGACAAGATGAAGGGCTTCTACAGGAGCAAGTACTTCGGAC
CGAACGGCGAAGAGCGCTTTGCGGGCGTCACCCAGTTTGAGGCCACCGATGCCCGACGCTGCTTCCCCTGCTGGGATGAG
CCCGCCATCAAAGCCACCTTCGACATCACACTGGTGGTGCCCAAGGACCGGGTGGCTCTGTCCAACATGCCGGTCATCAA
GGAGGATTCCCTTCCAGACGGTCTGCGACGAGTGCGTTTCGACCGCACGCCCATCATGTCCACCTACCTGGTGGCCGTCG
TAGTGGGCGAGTACGACTATGTCGAGGGCAAGTCCGACGATGGCGTCCTCGTCAGGGTTTTCACACCCGTTGGCAAACGC
GAGCAGGGCACCTTCGCCCTGGAGGTGGCCACTAAGGTGCTGCCGTACTACAAGGACTACTTCAACATCGCCTATCCGTT
GCCCAAGATGGACCTCATCGCCATCTCCGACTTCTCGGCCGGCGCCATGGAGAACTGGGGCCTGGTCACCTACCGTGAGA
CCTTCGTGCTAGTGGACCCGAAGAACACCTCGCTGATGCGCAAGCAGTCGATTGCGCTGACAGTGGGTCACGAGATTGCC
CATCAGTGGTTCGGCAATCTGGTAACTATGGAGTGGTGGACCCATCTTTGGTTGAACGAAGGCTATGCCTCTTTCGTGGA
GTTCCTGTGCGTACACCACCTGTTCCCGGAGTATGACATCTGGACGCAGTTCGTCACAGACATGTACACGCGAGCCCTGG
AGCTGGACTCTCTGAAAAACTCGCATCCCATCGAGGTGCCCGTGGGCCATCCGTCCGAAATCGACGAGATCTTCGATGAA
ATCAGCTACAATAAGGGTGCCTCGGTGATTCGCATGCTGCACGACTACATCGGCGAGGATACCTTCAGAAAGGGCATGAA
TCTGTATCTAACTCGTCATCAGTACGGAAACACCTGCACCGAGGACCTGTGGGCTGCTCTGCAAGAAGCCAGCTCCAAGA
ACGTGAGCGACGTGATGACCTCGTGGACACAGCACAAGGGCTTCCCAGTGGTTAGCGTCGAGTCAGAACAGACCAGCAAG
AATCAACGCCTGCTGCGCCTTAAGCAGTGCAAGTTTACGGCTGATGGCTCACAGGCGGACGAGAACTGCCTATGGGTGGT
GCCCATTTCGGTGTCTACCTCAAAGAATCCCACAGGGATCGCCAAGACCTTCCTGCTGGACAAGTCCTCAATGGAGGTGA
CCCTCGACAACGTCGATGAGGACGATTGGATCAAGATCAATCCGGGAACAGTGGGTTACTATCGCACCCGCTATTCGCAA
GAAATGCTAGAACAGCTGATGCCCGCAGTAGAGAAGATGGAGCTGCCGCCTCTGGATCGCCTGGGATTGATCGATGACAT
GTTTGCCATGGTGCAGGCGGGTCACGCAAGTACTGCCGATGTTCTGGCTTTGGTTGATTCGTACAGAAACGAGACGAACT
ACACGGTGTGGACTGCCATCACCAACTCGCTCACCAATCTGCACATTCTTATCTCGCACACCGATCTTATGGAGGACTTC
CATCGCTTCGGACGGAATCTGTACGAGCCGGTGGCCTATCGCCTGGGCTGGGAGCCACGCGATGGGGAGAATCACCTGGA
CACATTGCTCAGGAGCCTGGTGCTCACACGACTGGTCTCCTTCCGCAGCAGCGATACCATCGAAGAGGCAAAAATCCGCT
TCCGCAGACACGTGAACGGCACAGAACTGCTACCGGCGGATCTGCGCACCACTTGCTACAAGGCCGTACTGCAAGATGGC
GACGAAAAGATCTTCAATGAGATGCTTGATCTGTACAGGGCCACCGATTTGCACGAGGAGCAGGACCGCATCTCGCGAGC
TCTCGGTTGTTGCGGAGATTTAAAGCTGTTGCGTCGTGTCATCGACTTTGCCATGTCGAGTGAAGTGAGGGCTCAGGACT
CTGTGTTTGTAATTGTCGCCGTGGCCATCAACCCCAAGGGACGTGACATGGCTTGGGAGTTCTTTAAGGAGAACAACAAA
CAGCTGCTGGAACGCTACCAGGGTGGCTTTCTGCTTTCCCGTCTGATTAAGTATCTGATCGAGAACTTCGCGTCTGAGGA
GCGCGCCAAAGAAGTGGAAGAGTTCTTGCAGGCCAATCAAATCCCCGGCTGCGAGCGTACTGTATCCCAGGCGGTGGAGA
CCATCCGACTCAACGCGGCCTGGCTGCAGCGTGACCGCGAGCAGTTGGCGATCTTCCTCAGGGATGACCAATAAACGTTA
TTGTGCTCGCCAACATACCAAAGCATTTAACAACAATCACCAGCAGTGTAACCACAGCAACAGCAACGCCAACGCAACAC
CATCATCAGTACCAGTAACCCGTAACCAGGTCATCGTAAGCTACAGCTAATATATCCATAACACCACACGACCAAAGCCG
TCACAAATGAAGAAAACAAAAGGCAAACGTACTCCTAAAGATGAGAGCCAAATAGGTTAGCTTTAGTCAAGGATCCACCT
ACAGCCACTGCTCCAACCGGGCGTGCTTACATATATCGATTGACTACTCGCCCCAGCACATGGGACTTGGACTTGGACTT
GGACGCAGACCCCTAGCAGCATTTAAGACCCCTAAGCTTATCGAATGTGTAACTCCATTCAACATTTCTATCCCCTAAAA
ATTATGTACGTGTTGAGTTTATTACAATAAATAAATGCAATTCCTAGTAAGAGTCAAAACCAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: AT_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

    (Stapleton et al., 2002)
    Sample preparation

    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

    Protocol

    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    AT
    (Stapleton et al., 2002)
    AT_cDNA
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 1 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)