FB2026_02 , released June 18, 2026
Clone: Dmel\AT01532
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\AT01532
Species
D. melanogaster
Name
AT01532
FlyBase ID
FBcl0026795
Feature type
cDNA_clone
Computed gene(s)
Dmel\Chro, Dmel\asRNA:CR45968
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      testis
      (Stapleton et al., 2002)
      seminal vesicle
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,364
      NCBI
      BT132658
      Full Length sequence dataSequence Downloader
      CAATAAACAAAAAATTTCAGTGAAGTTGAATTTATTTATATTTTCTGAAACTTCGCAATAAAAAAGCCCACAGTGTCCGC
ATTTGCTCCGCTTGGACCGAAGCGCTGCAACAGACGAGATTTACATGCCTCGAAACCGGGAAGCGGAAGTCCGGGACCGC
CCCACCGGGGAGTGGCATCTGTTAGCCGCAGCTCGACAAATTTTCTTAGTGCCCAGGCCACAAGACATTCGCACTCATCC
TCGGTGACGGTGCACGTGCTATATACAAGGATGCCGCCAGGACGAACGAGCTGCACTGCCTGTTCAAATAGCCGGCGCTG
AATATGGGGATATGAGGACAAGACCTTAACCTGTTTAATGCTGCAGGACAACTGTGGCCGGTTGCCCAGCCCACTGCAGG
GTGCATCGAGCAAGATTCGGTCAAAGCTTTCACATGGAAACGGAGGCCCAGTGAATTCTCCCACGGGTGCAGATGGAGCG
TCCGCTGCCACTGCCTCGGTGGAGTTAAAAACATGCGCCGTTATGCTTTTGTAGTGTCCCAATTTGGGCAGCATGCTGCG
GACGCGACTGGCCGAATTGTCTAGGGCCACCACAGATCCCCGATCGCCCATCAACTCGGCTATGTGGCTTGTTTTGTTAC
CCGGAGCGGCACACATGTCCAGTATGCGCTCGCCGGGCTGCGGATCCAGGACACGGACGCAAACAATAGAAGGCAGGTTT
TGCAACAGGCCGTCTTCGCTGCTTAGATCCCCCAGCGTGGGCACTCCGCTGACGTTGGACTGCATTTCCACGGCCACTCC
AGAGGCGGGCTTCTCCGCGTTGTTGAACAGCTGATACCGCTGCATTAACACCTTGCCTGTACCCAGGAAAACCTTATTTG
GGCTTTCGTACCGCTTGACCGTCCCGCGCTTGCATTTTCCATCCAGATCTGCGTAGACATTGACGAGTTCCTCCCGCTCA
GTGTTTGACTCCATAGCTAAAACACCGGGGGCGTATATGTGGGCGCCTCGCAGTAGTGCAGCTCCGCAGTTAGTGTCAAC
CACTACCTCCTTGAGCTCGGAGTCGGCCACAGCTTTGGTGAGCTGTGAGTCCAGCGGATCAATGCACAACATCTCGGGTA
GGTTTGGCAAGCAGTAAATCCTTGGAGATTTTACACCGTAGCGAATGGCGAGTTTTTCTTCTAACTTTTTCCGAAAGTCG
TCAACGGAACACCTAAGAGTGTTCACCCTGTAAGTGGTGACTTTGGGAGTAAGGCACAGCCATTCCAACAGCGGAGCAAG
TGCAGCAGACTTGACAAAACCAGGAATTAACAGTTAATAAAATTTGCAAGCCAGTTTTTGTTGTAAAAAAAAAAAAAAAA
AAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)