FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\AT07729
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General Information
Symbol
Dmel\AT07729
Species
D. melanogaster
Name
AT07729
FlyBase ID
FBcl0027747
Feature type
cDNA_clone
Computed gene(s)
Dmel\Klp59D
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI21273
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage | male
    testis
    (Stapleton et al., 2002)
    seminal vesicle
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,518
    NCBI
    BT001278
    Full Length sequence dataSequence Downloader
    ATTGTCAGTAGCCCCTCAAACTCACACTCGACAATAAATTACAGAGGCAACATCTGGTCATCATTCGCTTGTGAACTGGA
AATCAAGAAAAATATATATAATCTGAAAATCCTAAAAAAAATACATCTTTTATAAATCGAGAGCTGTATAATCCGTTGCC
ACCAGACATGGATCGCATCAAAATTGGCGAGCAGCTCCTCTTCCAGAGGAGTGACGGGCGTGTCCATCAGGTGGTCTGCA
CTGCCAAGAACCTGGAACGGGATTCCATCACGGGCGAATGGACCGAGGGCACGGTGGTGAAGGGTAAGGAAGTGCCATTG
AGCACGCTGATTGGCATCAACCATCATATATTCGCGGAGAATCAACCGCCGATATCGAAGCCACCGAGCTCCTTGCTGCC
GAAGCCGCGAGGCTCGAGTCCGACCGGAGGACCTAAGAGCCACACTGGAAATCCGTATGCAGAGCGCATGAGAGTACAGG
ACGGTCGCAGTAAAATTGCCACCAGAAAGACGGATCCTGCAGCGGTCGGAGCGGGAAATCATGAGCTGGGTAGAGCGCAC
ACCCCTCGTGTGCCAGCCAGTAGGCCGGACCGGGATGCCAGCCCCACCCAAGGTCGCAAGTCACAGGGTGGCAACAATAG
CAACCAACGCTTTTCCAGTGTAGTGAGGGAGGTGAATCGCATGAAGGAGCAGAGGGAGAAGCGAAGGGCTCGCCAGGCGG
AACAGCTCCAGGAGAAGGATGCACTGCGTCGCAATAATCCGGGGAATCCTAACTGGGAGGTGTCGGTGATGCTGCGCCAA
TACCGCTCCACCTTGATCTTTTCTCCACTTCGATGCCTGGATCCCAATGGAGGCACTGTTCAGCAAATTACGGTGTGTGT
GCGAAAACGACCCATGAGTCGCAAGGAGGAGAACTCCAAGAACCTGGACATCATCACAGTTCCCAGTGCCGACAGCCTGA
TCGTCCACGAGTTGCGCCTCAAGGTGGATCTCACCAAGTTCCTGGAGCACCACAAATTCCGTTTCGACTACACGTTCGAC
GAGGAGTGCTCCAATGCGCTGGTCTACGATCACACTGCTCGTCCGTTGATCAGAACCATGTTCGAGGGCGGCAACGCGAC
TTGTTTCGCTTACGGACAAACTGGCAGCGGAAAAACGCACACCATGGGCGGAGAATTCTTCGGAAAGGTTCAGGATTGCG
GTACCGGGATCTACGCCATGGCAGCTCGCGATGTCTTCGAGGAGGTATCGCGCCCGGAGTACCGGCAAATGGGTGCCAAG
ATTACGTGCAGCTTCTTCGAAATCTATGGCACCAAGGTGTTCGATCTCTTGCTACCCAACAAGCCCATGCTGCGGGTCCT
AGAGGATGCCAGGCAGCAGGTCGTGGTGGTGGGCCTAACGGAGATGCCGGTGACCAAAGTGGAGGATGTCCTGAGACTGA
TTGAGCACGGCAGCAAAGAGCGCACTTCCGGCCAAACATCGGCGAACGCCAAGTCATCGCGTTCCCACGCCGTCTTTCAA
ATAGCACTCCACTTTCCCGATTCCTGGGGCCCACACGGCAAGTGCTCCTTTGTGGACTTGGCGGGCAATGAACGCGGGGC
GGATACGCAATCCGCCGATCGTCAAACTCGCATCGAGGGAGCCGAGATCAATAAATCTCTGCTGGCCCTCAAGGAGTGCA
TTCGAGCCCTCAGCCGGCAGTCGAGTCACCTTCCCTTCCGTGGCTCCAAGTTGACCCAAGTGCTGCGCGACTCCTTTGTC
GGCGGCAAGAAGAACAAGACCTGCATGATTGCCATGATATCGCCATCCATGAGCTGCGTGGAGAATACGCTCAACACTCT
ACGTTACGCAGACAGGGTTAAGGAGCTCATAGCCAAGGAAGACGAACACTTGCAGTCCGTGGAAGGGGATGGAGAGAAGT
CTCCCGATCTCAACGAGGAATCAGAGCCAGAAATGATGGCCGACGAGGAGGGCGATGAGGAGCCGGAGGATGAGGACAAT
CAGCATCTAACCATATCCTCGGAGGAGGCAAGCTCCTACAACAACATGAGTTATGACATGAGCTTTAACCACACCCTCAA
CATTCTGGGTCCCTCAAGGAACGTGGATATCCAAGAGGTGGCCGAGCAGCACGCACTTTTGGTAGAGAATCTAGAGACTT
ACGCTCACAATTTCCGGCAACTGAAAACGGATAAGGAAATAGAGCAGTACACCCAAAATTCAGAAAGCGCATTGATGAAA
CTGCTAGCAATGGTGAATAGAACGCGGGATGTAACGCACAATTACAACACTCAGAAATTATTGAAGGAAGAAAACCAAAA
TGCATATAAGAAGGGCGAGCTGGATGAATCCGAGTAGATATTCGCCCACCACCTACTAATAATCCTAGCATATGAATTTA
TTTTCTACCACTCTGTATCTTTTCAATTGCGTTTGGAAGACCCCCGAATGGCTTAGATTTCGATTCGATAAACAAGCAGT
TAATGAAAATTCAAGCGTGAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: AT_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

    (Stapleton et al., 2002)
    Sample preparation

    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

    Protocol

    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    AT
    (Stapleton et al., 2002)
    AT_cDNA
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 1 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)