FB2026_03 , released September 17, 2026
Clone: Dmel\AT12771
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General Information
Symbol
Dmel\AT12771
Species
D. melanogaster
Name
AT12771
FlyBase ID
FBcl0032127
Feature type
cDNA_clone
Computed gene(s)
Dmel\Tsp33B
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      testis
      (Stapleton et al., 2002)
      seminal vesicle
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,129
      NCBI
      AY075178
      Full Length sequence dataSequence Downloader
      GTTCAGAGCTAAAACCTCGTCGACAAGCCAAACTTTGGATCGATGCCCACGAGGATGACCAAATGCGCCAGCCCTTCCGC
CGAGCCTCCGTCTACCTCCACCTGCTGTTAATAACCGAGGCCGTGATCGGACTGCTGATCCTGGTGGTGACCGCCTACTA
CCACACCGTGCTCACCGGCTATCTATCGGATATTGAGTGCCGCCTGGTCTACGGCTATCTGTTTGGGATCTACGTGTTTG
GCGCCCAGGTGGTGGTGACCTTTCTCTGCAGCATTGCCATGTGGCGGCGAATTTGGCGGCGTCGCTGCACGCCGAACATT
CGCCTCTTGCTATCCGTGTGGGCCTTCTACTCCTGCGTAATCATTGCCTCCGGTTTCGGGTGCGTTTGGAATCTTTACCG
GGGTGTCGATGTCCTCGAGAATGCGGCGGACACCTCGCTGACCCGCGGCATCGACATGTACTACTCGTGTCCCGAGTGGA
AACTCCTGTGGGACGGACTGCAGTGGCACAAGGAGTGCTGCGGGGTGCATGGCTACAAGGACTGGATGAACGCGGAGTGG
ATGCCACGGCGGGAGAATAACTGCACGTCGATGGTCCTGGCACCCTTCGCCTGCTGCAAGCGCTCCTGCGACAGCTGCTT
TAACAATTTCCTGCCCAGTGAGGGGCAGTCCATCGGTGGCAATAGCCGGCAGCCTTTTCCCGCTCTCACCGTGGACTCAA
TCAATGCGAACGGATGCTTGCCTGCGTTCGTTAGTGCGGTGTGGAACTGCTTTTACATCCTGATGGCGTTATGGGTCCTG
GCCCTTAAGTTTCTGATCGTGCTTTGTTGTATGACCAAGTTCATAGTTCACCGGCAGAACGAGGGCGATGGCTGCGATAA
TGTGGGGCTCACGGACGATGATGGCCATCCCTTGGTCGTGGTGAAATATCCGTGCAACGTGCGCTGTGTGACAATTGCCG
AGGATGATCTGGTATCCGACAACGTTCCCGATATTAACTACTGCAATTGCACCGAAATGGACGACGAACCGTGTGGATAC
TAATATTGTGCTTAATTTTACTTTTATATTTTATATTAATTTAATTAAATTTACGAATTTGAGTTAACTTCAAAAAAAAA
AAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)