FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\AT14086
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General Information
Symbol
Dmel\AT14086
Species
D. melanogaster
Name
AT14086
FlyBase ID
FBcl0034131
Feature type
cDNA_clone
Computed gene(s)
Dmel\Nep5
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Stapleton et al., 2002)
      testis
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,551
      NCBI
      BT001312
      Full Length sequence dataSequence Downloader
      CGCTGCTTTAGACCAGTCACCCGCTGCACTGGCTCCACTTCGAGTCGATTTTTCCAGAGTTCTAGCTCCAGCTCTCGTTT
CGGCCCCACATAATGCCAAATTATGCAACTGCCTACTGTTGGCAGTAGCGGATAAAAAAGGCAAACATTCCGTCTAATGA
GCAAACGATACAACCGATAAACCGTTTCCACTTGGTCGCCAACTATTCCCTGCGTCAGCGCAACATGTGGGCGCTGTGAC
GTGCATTCGTAAAAGGCTCATCACCCGGCGGCTCATTGATATGCCCCGAAGTGTCTAGGAAAATCACCGGCAGACTGCGT
TAAAAGAGCAGAAAAATCACACGCAATATGCAATAATCAGGGGCTTTCCACTGGACTGACTCATGACATGACTATGGAGG
ATCGCAATCGCATCTGGACCACGGGCAACATTAACCATGGCTTCTTTGGCGACAATCTGCAGCAGCAGCCGTTGCCCCTG
CAGAGATTGCAGACACCAGGTAACCTCAGCCTTCTTTCGGCACCGATTCGAGCCAATGGCAGTGGCAATGGAAACGCAAC
TGCCAATGGACATGGACAGAATTCGGCCACGGAGAGTGCGAATGGAAAGCAGCTGCCATACGAGCCCATATCCCCAAACC
TAATTGGCATACAATCCAAGTTTCGTCGGAGTTATTACCACAAAGTGGTGCTTGGCGGACTGCTGGCCCTTGTAATTATT
CTGCTGATAGCAATCATAGTCATAAGCCTAGGCCTTAAGAAATCCAGCTCAATTTGCCGTAGCAAGGAGTGCCTCCGCAC
AGCGGCCAGCCTCATCTATGCGATGGACGAGCAGACTGATCCCTGCGAGGACTTTTACCAATTCACCTGCGGCAGGTGGG
CCAACGAGCATCCGCGTCCGGATTCGGTGACCTCGAACGATTGGTTCCGGGAGCGTCAGGCGCACATAATGCGTGTGGTC
CGCGAGTTCCTCCGCTCGAATATTACCAAATCGGAACCAGAAGCAGTGGGCAAGGCAAAGACTATGTACAGAGCCTGCAT
GGATACGAAGCTGCTGGATAAGCGTGATCTGGAGCCGCTGATAAACTATCTGCTGCGTTTTGGACTACCCGTTCTGCCCT
CCGCCTTGAATCTCACCCTGGGAAGTGGTTCCAAATATGCCACAGAGGCAGCCAATGTCAAATACAACTGGCTGCAGTCC
ATTGTTTCCATTAAACAACACCTCACCATGGACCTCATCATAGGATTCGATGTCTTTCCGGATCCTTTCAACCGCACCAT
CAACCGCATTGCCCTGGGAACTCCAGAGACTGATTCGGCGTTTCCTTTCAACAACGACGACTCCCACAAAATGCTCCGTA
AGATCCATCGCAAGACCATTTTTATGCAAAACAGCGATGATGAGGACGACAGCGAGGATGACCGGGAGAGCGAGGAGGAG
GAGGCAGCCAAGCAGACTAGCACGGGAATGACCGCCTATCTGCATTACGTCCGGAAAGTGATCGAAAAGTATCTGCTCTA
CGTGGATCCGAATGTTAACCAGGAGGAGGCCACTCTGGGCATCACCGAGTTGGTCAAACAGGGTGTTCGAGTAGCAAGAA
AAGTGCATGAGTTCAAGGAGGAGGCTGAAAACATGACCAAGCCATCGAAGAACCCGGCAGATGACATTGTCTACATCACC
CTGCAGGATCTGCAGAACCAAACTGACAAGAATATAGCACCCAAGACACTGCCCATCTGGGTTCGTTACATGGAATTGAT
TCTAAAGGGCACACGCCACGCCGGCAATATCCAAATGACCCAGAATCTGACAATAATAACCAGCCAGGCAGACATTACGT
ATCTGCAGAACATTGTGGAGTATCTTGACGACACTCCAGCTTCCCATATCGAATCATACCTGTTTCTCAGCACCATCGAG
GAGCTGGTCCTGCATACATCGAGTTCGATGCGATTGCTTCACTCAGAGTATATGCGGGTGGCCATTGGAACAGAGGGCAG
CACGCCGAGATCCCTCTACTGCGCAAATGGTGTAAACAGTTTGCTCGGAATGGCAGTGAGCTATGTCCTGGCCGACGCGG
ACTTCACCAAGGAAAAGCTGCCTAAAGTTGAGCGGATGCTGAGTGATATACGACGCTCCTTCGATCGCCTGGTAAAGTCC
ACCTCTTGGATGGATGCAGCTACCAAGCGGAAAACCATTCAAAAGTCAGCTGAGATGAAGAGCTTTATCGGATTTCCCCC
TTGGCTGCGAAATGCCAGCGTATTAAATGCCTATTACGAGGGGGCAGAGGTGAATGCCAGCACCCACCTGGAGAACCTCA
TGGACTTTGTCCACTGGCAGATGATGGACAAACTGAACGAAATGGATAAGCCAGAGCCCATTGGCTGGGCCACTTCCCCT
TCAAATGTCAATGCGTTTCATACGTTTCAATCGAATGCAATCACTGTGCCCATTGCCATTCTGCAATACCCGTTTTACGA
CCTTGGTCTCGAGTAAGTTCACCCAGGAATAGCACTAAGCACTAAAAACCGAGCACTGAGCACACAGATAGCCGAGCAAA
CAGACACCCAGGCACGCCCGCGCACCCGCACCTAGCACTAATCCCCTCCTGGAATGGCCATAACCCGTGTGCTGCTCTGT
GTCCTCGGCAGGGCGCTCAATTACGGCTCGATTGGAACCATCCTGGGCCACGAACTCACACATGGCTTCGACGACAGCGG
CAGGAGATTCGACCGGGCAGGGAACATGGTCGAGTGGTGGTCAAATAGGACCATTGACGAGTATGTCAATCGCACCGAGT
GCTTTGTGGAACAATATAGCCGCTACCACCTCGCAGACATTGATGAATATATTGATGGCGAACTGACGCTGGGCGAGAAT
ATCGCAGACAACGGCGGAATGCGGGAGGCCTTCTATGCGTACCGCCTTTACGTGAAGGAAGTGGGCCGGGAGCGGTCCAA
GTTGCCCGGCTTGGAGCACTATTCCCATGAACAGCTCTTTTTCATCTCGTTTGGGAATCTGTGGTGTGAGACCTACACGC
CGGCAGCATCGCGATACGCGCTCAGCGATTCCCATTGCCCGGGACAGATGCGTTTGCGGGGAGTGCTCTCAAACTCGGAG
GAGTTTGCCAGGACCTTCAAATGCGCCCGAGGAACGCCCATGAATCCCGACCAGCCCAAGTGCCGCATTTGGTAGTGGGT
GTGCTCGACGAATAAGCAATTGCAACAGGAACAGTCAAGTGATTTTTCTACCCGTTTGTACTTAAGCTAGCCATATGATT
AGATCAAATATACAGGTGTCTTTTGCACGTTTCTGGATGCTGGCGCAATAAACTTAATGGTAAAACGCTTCTCAATCCGA
GGTCGCAGCTCATAAGTAGGCTTCGTTTTAAGTGGAATAGTTTAGATAGTTCATTGTTCATTGCATCATTTTTGGCTTTA
TGGAAACTTGCTAATTACGATCAAAAATACTTGCATGTGAATATTGTATATACTTTTAATTAATTACTTTTAATATACTT
TCATTAAAAATAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)