FB2026_02 , released June 18, 2026
Clone: Dmel\AT29464
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General Information
Symbol
Dmel\AT29464
Species
D. melanogaster
Name
AT29464
FlyBase ID
FBcl0038099
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG5653
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI24493
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage | male
    testis
    (Stapleton et al., 2002)
    seminal vesicle
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    1,493
    NCBI
    AY075271
    Full Length sequence dataSequence Downloader
    CAACGTTTCAAAATGGAAAAATGCCGTACCTCCTCTAGGATAATCATAATCGGAGCAGGAGTTTCGGGCATAGCCGCCGC
CACTCGACTCCTGCAAAACAATTTCCAGAATGTTCAGATCCTGGAGGCGGAGGATCGCATTGGCGGGCGGATCAACACCG
TGTATTTTGGTGACAACGTCATCGATTTGGGAGCCCAGTGGTGTCATGGAAAGCAGCAAAATTGTGTATACGACATGGTC
AAGGATATGGGTATCCTGCACGAGACAGGCGACTATTTCAGTCCCATAAAGAGGGTGAGATCCAACAAGGAGGTGGTTCC
ACATGAGTTGGCCTGCAGGATACACGACATTGCTGTGAAGAGCATGCCGAGTGGACCTCACCCGGTTGTGGGATCCTTTG
GCACCCACCTGACTCAGACCTTCTGGCGCAAGATCGAGTCCGAGCTACCCCAAGTGAATAGGGATGTGGCTAGTGAGGCC
TTGAATACATTTGCGAAGCATGAAAGCTCTATTATCGGTGCGGACAATCTTTTCGAGGTCTCCGTTAGGGAACACATCGA
GTACCACGAATGTGATGGCGACAAGCTGCTCCACTGGGGAACGAAGGGTTACCGGCGCTTCCTGCGGCTGCTGATGAAGG
TCAGCGAAGATACGCCAGAAGAGCTGGGACTACTGGAAGGACGTATTCAACTTGATAAAAAGGTCATCAAGATTGAGTTG
GCGTGCCCCCGTAAGGTAATCCTGCGCTGCCAGGATGGGGATTACTTCGGGGCGGACCACGTCATCTGCACCGTTTCCCT
GGGAGTCCTGCAGGAGCAGCACGAGAAACTGTTCGTCCCGCCGCTGCCCGCAGCCAAGGTGAATGCCATTCGCAGCCTCA
CCCTAGGCACTGTGAACAAACTGTATTTGGAGTATGAGAAGCAACCTCTTCCTGACGGCTGGGTGGGTTTCTTCTGTTTT
TGGCTGGAAGAGGATCTGGTAGAGCTGCGAAAGACGGAATATTTTTGGGTGGAGGGTATAACCGGTGTACACATGATCAC
TTGTCAGCCCCGAATGTTGATGGCCTGGGTGAATGGTCCGCATGGACGCCACATGGAGACCCTGTCCGATGAGAAAGTCC
TTGAGGGCTTGTACTGGCTTTTCCGAAAGTTCCTCACTTTCGAAATTCCGCCGCCAAAGCGTTTCGTTCGCAGCTCGTGG
TTTTCGAATCCGAATTTCCGTGGCAGTTGGAGTTATCGTGGAGTTATGGCCGATGAAAGGAATACGGGCCCCTGGGATCT
AGAGTCTCCTGTGTTGGGCGAAGATGGTCATTTAGGCCTCCTTTTTGCCGGAGAAGCGTCTAGCAGGAATCACTTTTCCA
CCGTTCACGGAGCTGTGGAGGCGGGCTATCGGGAGGCAGACCGGCTCATTGATCATTACACCTCATGCAGTGTCAGTGCC
TGAAAAATATATTTTTTTATCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: AT_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

    (Stapleton et al., 2002)
    Sample preparation

    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

    Protocol

    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    AT
    (Stapleton et al., 2002)
    AT_cDNA
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)