FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\AT17592
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General Information
Symbol
Dmel\AT17592
Species
D. melanogaster
Name
AT17592
FlyBase ID
FBcl0044045
Feature type
cDNA_clone
Computed gene(s)
Dmel\f-cup
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Stapleton et al., 2002)
      testis
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,392
      NCBI
      BT012334
      Full Length sequence dataSequence Downloader
      AAAAATTCTTTAGTTCTAAAATTGATTTCGCTATCAACCGACCGCAAAGAAGTCGCGCATAAAACTCTTGAAGACACCGG
CTGCCATTGCGTGGATAATCCAAGGAAGCCGAGGCAGAAGCCAAACGATAGACAAAGGGATCCCCAACGAGATGATGAAC
TTAAGCAACGTAGAACTTGGCTCATTCCGCGGTGCCCAGGAATGTGACCAGGCATCCGCATTTGGCGGCGACTTCGACTT
TGGCCATTCCTGCTTCACCCACTACGGCTGTCCAAGCGACATGAGTGACAAAACCGGCCGAGGACGGAGTAACCCCTCCA
CCAGCCGGATTCCTCGCAGCGCACAAAGTCCGCGCCAAGCCTCAGTGGTCCACTCGCCCAGGACAAACTGTGCCTTTCGC
CCGATTTTTCACGAGAGGAGCACTCACGAGGACGACCAGGTGCTCACCCAAGAACTATGGAAGCTGGCGCGTAAATCGGG
CACCCTGAATCTTTCCAACAAAGCATTGGCCAGAGTGGTGACCCAGGAGCAGCTGCGTCGTTGGAAGTCCATGGTCCCAC
CAAAAACAAGTCAGCCGGTCAGTGATCAATGGAGCCCCGGCCTCGTTGACAGCAGCGCCTTGTGGTTCCAGTTCATGTGA
GGCAAAAGCGATTTCGATCTCGAACTTGACCCTGGAGAATCCCAAAAATGGAGCGAAGCATATTTGCCGCTTCACTGACC
AGTATGCGGGGCTCGTAATGATCCATAAAGGTGCTCCAGGGGTGCGTCCCCAGATCAGTCATCGCTTGATTCAGTCTACC
CACTGAGCTGAGCGCCATCGGTTGTGTGACTCTACCGAAGCATCTCCCAAGATTGCGTCCTCCAGGAAACAGCTCACTTC
GTTCTGCATGTGCGGTCCTTATCCGCGGAGGCGTCGCTACTACCAGATTCGCGAATTCCAGATGGCCTCCTCGGCGGACG
AGCTGGAAAGCGAGGATCCGGACGATGAGCTGGAACTGGACGATTCCGAACCTCCAACCCGCGCTGAAGACTTGCCCAAG
CGACTGTACGACATTAACGAGGCGGATGCGGACAGCAAGGCCGTAAATCTAGAGCAGCTAACGATTAAAGAGGAGGATGC
CTGGTGGAACCAAGTGCCATTGAACAACCTGGACCTGAGCTCAAACACCTTAACGCACATATCGCCAAAGATCGAGAATC
TGCAATCACTTACCGTGCTTACGCTGCACGATAATGCTTTGGTGGAGTTACCACCGGAGATCGGCAAGCTGGAAAAGCTA
GTGCGCCTTAATGTGAGCCACAACAAACTCAGCCAGCTGCCGCGTGCGATGTACAGTCTACCGGAGCTGCGGCACCTCAA
CATCTCGTACAACGAGTTCGTCGAACTTAATCCGGACATCAGTGACCTTCACATGCTTGAGTTTTTGGACGGTGGGCATA
ACAATATTCAATCCCTGCCCGGTGGCATTGGGTTCCTGGTGCGTCTAACTGCGCTCTTGTTGCCCTATAATCACATCAAG
GAACTGCCGCCCGATCTTGTTAACATGCGCTCTTTGCAAAAGATTGATCTGATGCATAATGATCTGACCAGCTTGCCGGA
GGATATGGGCCTTTTGAGAAAGCTGGATTGCCTTTATCTGCAGCATAACGACATACTGGAACTGCCCGAGTTCGAGGGAA
ACGAGGCCTTGAACGAGCTACATGCCAGCAATAATTTCATCAAGATCATACCAAAAGCTATGTGTAGTAACTTGCCACAC
CTGAAGATACTGGATTTGCGGGACAATAAGATCACGGAGCTGCCCGACGAGCTGTGCCTGCTGCGCAATCTAAACCGTCT
CGATGTGTCCAATAATACGATCAGCGTGCTGCCAGTCACATTGTCCTCCTTGGCTCATCTGATTAGCCTGCAGGTGGAGG
GAAATCCCATAAAGACAATCAGACGCGACATCCTACAGTGTGGAACAACGAGGATCTTAAAGACTCTGCACGATAGAGCT
GTGGCGAAGGCCAAGGAGGAGGGAGGTGGCGTCGACGACGCTTCCACGTCAGCGGGCATCAGTGTTACCCGTTTGCGTGG
TGGCCAAATGGACGATGGTGATATACCCGGAAACTTTCCGGATAGCTTTCACCACCAGCAACAGCAGAACGGATTCCAAT
GTCCTTGTTCATATCCATGTCAGCAGCAGCAAATGCAGCAGCAGTTTTGTGTGTATGAGCCGCTCAGAAACTGTCAGGAA
TATGATCGCCAGACCCAAGGGCGCATATATGAGCCCGAAAACTACCAACAGCAACATCAGAATGGCAGCTTGAGATCACT
TTTTGTGCAACAGCAGCAGCAGCGAATGGAGTATCCCTATCCAGGATACTTTATGTACCAACAGCAGCAGCAACAGCAGT
TCTCCTATGAGCAAGATCCATCCTCTCGCTCAGCTGTCCATCCACGTTGGGTGTAGTATACAAACTACGACATACACGTA
CATTAGCTGTCAATCTGGAGGAGCTCACGTCAGTGCCTGACCAGGTGTTCCAGATCGCCAGGGATGAGGGTGTGCATGTG
GTGGACTTTGCGCGCAACCAGCTGAGCACTTTGCCCAATGGACTGCAGCACATGAAAGATTTAGTTACGGAACTAGTTTT
ATCCAACAATGTCATCGGCTATGTGCCACAGTTCATCTCCCAGTTTACACGTATATCTTTCTTGAATTTGTCCAACAATC
TGTTGAATGACCTGCCCACGGAGTTTGGTGTCTTGAATACACTGCGTGAACTAAACATTGCAAACAATCGCTTTCCGTGT
ATTCCCAACTGTGTGTACGAGCTGCAAGGCCTGGAGATCCTCATTGCCAGTGAGAACCACATAAAAATGTTAAATGTATC
GGGTCTGCAGAACATGCGACGTTTGAGCACATTGGATTTGCGCAATAACGACATCGAGACAGTGCCACCAATTTTGGGGA
ACCTGACAAACATAACTCACTTGGAGCTGGTGGGCAATCCATTTCGACAGCCCCGCCACCAAATCCTGATGAAGGGCACT
GACGCCATCATGTCGTATTTGCGCGATCGCATACCCACATAGAAGAGCAATTCACACCGTTTTTTGAGAAACACTTCACT
AGCAATATGTTTATTTTGAGGAATAGCGTAATGGAAATTGATAGATCCGCTCGACCATGCCCACAAACAATCAATCTATG
GGTTGGCTGTTCGTGGACATGGATGAGAGGAGGCGTTACAAGTACAACATACGACACACACCGTGCATCAACCAACCAAA
CTAGTCTGCATGCCACAATACCTAATGCACGTTGTTAATCCTGGCTGAGTATCTGCAATAAATGGGAATAGTAGGGAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)