FB2026_03 , released September 17, 2026
Clone: Dmel\AT14886
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General Information
Symbol
Dmel\AT14886
Species
D. melanogaster
Name
AT14886
FlyBase ID
FBcl0045691
Feature type
cDNA_clone
Computed gene(s)
Dmel\qin
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      testis
      (Stapleton et al., 2002)
      seminal vesicle
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      5,222
      NCBI
      AY118283
      Full Length sequence dataSequence Downloader
      GTGGTTCAACGGGCCAAACAAAAACTAAACGACTATGCCACCGAAACGTTGGCCAGCATTTCGAAGCGATTTTCCCACCT
GCACGGCCTTCTCCAGGTGGCCGAGCTGCAGGTCATTGAGAAGCTGCGCGAGTCCAGTTTGCCACCACAATTGAAACTCA
ACGAAGCCATGGGCCAGCTGAGTGGCTACGAAACTGTCATCGAGCGATTAACAAAGAGTTTAAACAGTGGAGTCGGCAAC
GACATGGCCGTACCCAGAGACGTGTCGCTAAGATGGCTGCTCAAGCTCATTTGCGAACATCTTGAGAAGATTCCCACAAC
TGTGCAGGTCAGCAAAGTCGACGAAAATCCTTATCGCGTGACCTGTAGTCGATTCATGGACATATCCAACATATTCAAGT
GTGAATTCGTGGACCCCAATATACAAGTTTGTTTCAAGACCGACCTCGGGATTAGTAGAAGCCTTTCATCCTCGCCCAAA
GGAGAAATCACTTTTTTAACGTCTTCCAGTAGTAGCAAGGAAAACTTTGATCCACATTCACGCAAACTTTTATTAGCCCA
AAATTCGAAACTAAAGAAAGCCACCCAAGTGTCGCAAAGCCAAAAGAAACAGGGTAAAAATGCTACGGAGGTCGCCCAAT
CCAGTCTAAATTCAACTGCCGCCATTATCGATGAGTTTCCAGCTAAGGATCTAGATTTTCTCCACAACTTCTCTTCATTG
GACATCACCAAGCGCAATAAAACGACTGAAGACAGCAGCGATTGGTTCAAGACCGATACTCTGGTTAGGGTGCGCTCCGT
ACAATCCCCAGAGGATTTCTATGTTCAAGGCATCCATGCCGCCCAGCGATTGCGTGAGGAGCTGGATACATTTGCGCACA
CACTTTCGGACAGTTCCTCTGTTCCGCCGACTATCGTGGTGGGCCAGAACTACATTATCCACCACAAAGATAAGGATCGA
TACTATCGCGCCCTGGTCAGCCAAAAGCTGACCAATGAAAATTTGTACAATGTTTTTTTGACGGATATAGGCGTTCATTT
GCATGTTCGTTGTTCGGACTTCCGTGTGGTGCCGGAACGTATATCCCACCTGCCGTACTCCGCAGTGCACTGCAGTTTAA
GTGAGTTGATGCCTAAAAATGGTGAATCCGAGTGGGATAGTAAAGCCAGCGCGTTCCTTAAGCAGATAGTGCAGAACAAC
CCCGTGCGCGTGATCGTCAAGAAAGCTCTTACCTATGAACTGCATGGGGTGGACCTGATCACCAGCAATTACGATACTAA
CATCTCCGTGCGAGATTCTTTCTTATACTGTGGCCTGGCGATCTCCCGCGACGGTGCCCCTCTATGGCTGCCGCCTGCCC
CTACTGCGCTTCGTCTACCCAGAATAAGCTTCCGATTCGGAGACGTCTACATGGTTCAGATGCTGCACGTGGAGGACCCA
CAGGAGTTTTACGTCATGCGCCACGACTACGAGAAAAAGCGCTTATGGTTGCAATTCAGTCTGCAGGAAGCCATGGATCG
CATTAACATTAGCCAATTGCAAAACATCTTTCTTGGACAGCTGCATCTCGGTTGCGTCCTGCAGTCGGGTGGACAGTGGA
AGCGAGCTAGCATTGAGCAGATCCTTCCCGACGGTTATGTTCTCGTGCACCTGGTGGACGAAGGTCCAAGTCAGAAAGTT
TTCTGGGATCAATTATTTGTCCTCCCTCAGAAATTTTGGGATACAGGGCTAGCCATTAAGTGCTGCTTGGCGGATGTGGA
GACCAGAGCAGAGCACTCCTATACTTGGACTCCAGAAGGGACGACCCTTTTTAAACAGCTTACCTCGAATCCAAGGCTTT
ACATGGATGTTATTAGTTGCACGGAGGATCTGGTCTACGTGTCATTACATTTCGAGCGCTCCAACTCGGAAACCACAAGT
GTCGGAGTACAACTAGTGGCCCATGGACATTGTACATCAAGTGGTGAAAGCAGTAGAATGATCAAACCAACCGTATCCAA
TCGTTCTGTACGTTTTGATGAGGAAACCAAAAAGTTTATTGCACAGCAGAAGGTCCGGCCGGTGGAATTGTCGCCTTACC
GGATGCCCGATAATACCGAGCGCAACAAACGAACAACTGTCAATATTCTTTATGTTCGAAAGCCGGATGAGTTTTACGTG
ACCTTGCCCCATTTCCAGCAAGCCATCAATAACTTGCAGAAGTCGGTGCAAAAAGCAGCTGCCGCAATGTACCAGAATAT
GTTGCCCAGAACCGATTGGCAGGTGGGCGATATGTGTTACGCACGGGTTCAGGCCAATTGCGATTCGCAGGCTTTATGGT
ATCGTGGAGTCGTAACAGGAGTCATACCACCTGGTATTACTTGCCCAATTGTACGATACCAAGTGCATTTGCGGGATTTG
GGTGAGCTGATCGATGATGTGCACAGTTCTTCCCTGGCCAACATCGATGAGGCGGACATGCGTATTTCAAGCAGCGCAAA
GCGTTGTCATTTACACGGGATTCGTCCAATTGGTGATGAATGGTCCAAAGACGCCATTGATTTCTTTATGGATCAGTTGA
AGGCATATAACGAAATCCACGTGACGGGGCGTGGGCGTACAGAAAACTCTTTGAGCGTCATCCTATGGGGCTCCCTTTCC
ATACTCACTGGACCATTTTCGCCAGCTACTATAAAGTACGTTAATATCAACAAGGCTCTTCTGATGGCCGGAATGGCAGA
AAAGGATCACAATTCGGATAGCGAAGACGACCAGCAATCGATGCCGGAAAATGTAAGCGTTAACTCAGAAGAAGCTGCAA
AAGCCAACGACTGGGAATCCTGTTTAAGCAAAATTGATGGAACTTCTAAGACAAACGACTCATTGAATCTCATTGAATCA
CGAAGCGTGACAGTCGGCTTTGAGCACAACGAGGACATGCCTCCACTAGCTCTGCTCGAGGATTTAGGAAATACCAAGAA
TACAACTGGAGAGACAACACCACCTGCTGGTTGGACTACACGACGAAAGTGCGATAAGTCCGTATTCACGGCAATTGCTA
CTAATGTGACCTACGAATGCTGCATCTACTTGACTTTGGCCAGCGATAAGCCCTTCATCGAGCATATGGGCAACCTGCTG
GTACGGGAATACAAGCCACTAATGGATAAGCAAAAGGAGCGCTCTACCTCCTATACTTACAAAATGGGACAAGCGGTGGT
GGTTACCTATCACATGGACAACATGATCTACCGAGGTATAGTGCAGCGCCTGGAGAACAATCATAATGAGTACACAGTCT
ATTATGTTGATTATGGCAACATGGAGCTGGTCAAGGCGGATGAAATGCTTCCATATGCTCCGTTCCCGGACCTAAACGCA
ATGTGCTTCCTGGTGGAATTACACGGCGTGCGATCTAAACAAGGAAAGTATTCTTTAAAGGAAATGGATACGGTCCACCT
GAATTTGGTCATGAAACTAAGCGGTGTGCGTATTGTGGATGACGAAACAGTTGGCCCAAATAAGATACCCAAATGCCAGA
TTAAGGTTGGTAATGTTGACATTGCCACCATGATGATAGATTCTGGCATGTCTGTACCTATCGAAAAGCAGACGATCAAG
AATAACACAAAATATTTACCTTCCCAAAAATTTCTCGAGGACTTTAAAGTCTTTGATGAGCTTGACCACCTGGCCGCCGG
GGAGGGAGAACTATCAGACAAGGAAGCGCCTCCCACCCAACGTAAACACGATAGCATTTCATCGGTTCAGCAACCACCCA
CTAAGAAATTCCTGGTAGATCAGGAGGAGGTAGAGGCGTTTGAGTGTGATCAGGACTTTGATTGCCAGCAAGCCGCACAG
AAAATGCACCTTAACAATAATTTTTATCTCGGCGACTTTGGTAAGTACGCTGATGAAACAACCGCTCGGGATGAGGATGA
GCCCCACTCGAATTCCTCTAAAAGTATTGATATGGAAGTCTTTGCGAACGTCAATGACAGTCAGTTGGAGCCCCTAAATT
CATTCGCCACCGAACAGTTGATCCGCCGTATTGCACTGCGTCAACAGGAAATAAAGGACAGCATTACCTTTTCACCTATT
GACACCTCGTCGGTGAGGTCTTTTTACGATGCCAGTGCGAGCTTCAAGACCCTGAGCCTTCCGAACGGAGTAAAAGAGTT
CTACTGCACAGTGGACAATGTACTGTCGGACACGGAGTTGCAGATAGCCCCATGTCTCTCGGAGTTCACAAAGCATGAAA
TCAGCCTGATCCAGGAGACGAGCACTTTGATCAAGGACGCGGAACCGCTGATGGAGCCGAAGGTAGGCGACTTGTGTCTG
GCCCGCTATTCGAGAGACAAGCAGTGGTACAGGGCCAATATCAAGGAGATTTCACCAATCCTGTCTCCAACCAGTGAGCA
AGTCACAGTATTCTACATTGATTTCCATGACACCGAAAAAGTTTCGTTTAACCACCTAAAAGTAATGCCCAGTCAGTTGT
TCATGTTCCCGTTGCGATCGTTCTGCGTCAAATTGCACGGCATTAAGAGAAACAAGAGTTTTAAAGAAAAATCGGTGCGC
CAGGCACTGCAGGCTTGCCTCTGCCAGCATCCGCTGGTTTTCGCCCGGGTACACTATCCTCTCAACTATTACCCCAACCG
CCCTAATTGCGACGCTGATCTCATCGAAGTGGAACTTTACGAGAACAGGCACGAAAAAAAGCTAGTGTATCAATCTTTAT
GTGAAAATTGGATGTTCCTGAAAAAATCATAAGGCTACGACTAAAGCCGCATATGATGTAAGCCATTTTCAGTTTCTATT
TATTCATTAGTTTACTCCTATGCTACATAAATGAATATGTATGTTTTACTTCATATTTTAAGTTTCAGATGTTATAAAAA
ACACGAAATATGTTTATTCGAATTTAATGTCATTAAGGCTACGGCTAAAGCCACATATGATGTAAGCCATTTTCAGTTCG
TATTTATTAATTAGTTTACTACTCCTATGCTACATAAATGAATATGTATGTATTACTTCATATTTTAAGTTTCAGATGTT
ATAAAAAACACGAAATAGGCTTATTAAAATTTAATGTCATCCTTTTTTGATAAAGTTCTTGAAGTTTGAACTGATTTGTG
CTGTTATCAGTGGTGTCAGTCGTCCAGCAACCAGTCCCGCAAAAGAATCAATAAAAGGCGTTGATTGTTTTGTAATTATC
CATAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)