FB2026_03 , released September 17, 2026
Clone: Dmel\bs43d03
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General Information
Symbol
Dmel\bs43d03
Species
D. melanogaster
Name
bs43d03
FlyBase ID
FBcl0057173
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG4691
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    BS_cDNA
    • BS cDNA library of D. melanogaster, adult male, testis including ejaculatory duct and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Andrews et al., 2000)
      ejaculatory duct
      (Andrews et al., 2000)
      testis
      (Andrews et al., 2000)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      517
      NCBI
      BE975455
      3' sequence dataSequence Downloader
      TTTTTTTTTTTTTTTTTTATTTGTATAGTTCTTTAATACGAGCATGACACAACACATAGTTGGGATAGAATTGTCATATG
CTCTCAAAATGCGAAAGCATCTTTTGTTGCGATAGGCCTCGCGACATTAATTTAGTTCGGTCAAAACTTGGTAAAAATCC
TTTGGTGAAATCGAAGTTGATTAGCCAAATCGGCGGCGGTAGTGGCGCCACATATCGCAGATGGCCGGCTTGAGCAGGCA
GTTGCACTCGATGGGTCGAGGATCCGGGAAGGGATCGCGTCGACACTCGGAGAAACTTGGATACTTGGTCTTGGGTCTCT
GGCCACAACGTCTAAAGGGACGACGGCACTTGGGCGGATCGCGGGCGGCCACACAGCAGTTGGGCATTCTGAAGCGCGGA
CATTTCGATGATTCCTTAACGCAGGGTTTGATGAGGTTAAGCTCCAGTTTGCCCAATACACACAAATCCTCGGGGCAGCG
GGGCTTTTTCGACTTGCGCTTGCGCCGCGGCATCGGT
      5' Sequence
      Total bases
      543
      NCBI
      BE975456
      5' sequence dataSequence Downloader
      GAGCACATCTAAAACAAAAAAAATGCGGTGCGGTCAGTAGTTAAATTGCGAAATTGAAATCGAAGTAAGCTCGTGGCGTC
TTACCAGTTTTACCCAAAATTATCTCGTTTTGATTTGAAGACTCGCTGCAAAATAATTTTAATCAAACAAAATTAAAATG
CTTGCCAAAAGCAAACCGCTGATTGGCATGTCTCACTTGCTGCAAAAGCAGGTTTTGGGCTTTCTGCCTCCGTCTTCGTT
TCGCCACTTTAACTCCGAGGGCAATTTGTACTCCCAGGATGGCTGGGAGAGTGGCTATCCGGCGCCCTTGCTGCCGCGCA
AGGACCTAAAGCCGTTGGAGAAGAAAGACAGATCAAAGGTATACGATGCCTGTTGGCAAACGACGCGGCGGACCGAATAC
AAGTGCCGTTCGGATCCAGAGTTTCAGATGCACGCCTTTATCGATTCGCGCAAAAGTTGTTTAGAAGACCCCTGCGCCAC
CGAGATGTTGGCCATCGATCTCACCCACTATAAGCCCTCGGACATGGGCAAACGAAAGTATCC
      Library Information (1)
      Library: BS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from young adult male testes, seminal vesicles, and ejaculatory ducts.

      (Andrews et al., 2000)
      Sample preparation

      Adult male testes were dissected from 1-5 day old y[1] w[67c1] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles.

      Protocol

      Poly(A)+ RNA was purified from dissected testis. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.

      (Andrews et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      BS
      BS_cDNA
      References (1)
      Research paper (1)
      Andrews et al., 2000, Genome Res. 10(12): 2030--2043
      Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis. [FBrf0132348]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)