CGATTGGAGGATTCTCCGCGAGATATTCGCATCTACAGTTCCGATCGCCAAAAGTGCAATGTGAATCCGTGCAGGATCAA CAACGGCGGATGTGCCCAGAGCTGTCATCCTGCCCCGAATGGCAAGGCCGAGTGCAAGTGCGACGATAGCACCAAGGTGG TGAACGAGGGAAGGATGTGTGCCCCGCGAAACAATACTTGCGAGGCCAGCAAATTCTACTGCAAGAACGGCAGATGCATT TCGAGAATGTGGTCCTGCGATGGCGACGACGACTGTGGCGACAACTCCGACGAGGATCCCAACTATTGTGCCTATCACTC CTGCTCCCCCAACGAGTTCCGCTGCAACAACGGACGCTGCATCTTTAAGTCGTGGAAGTGTGATCACGAGAACGACTGCA AGGATGGTTCCGATGAGCTGGGCTGCGTCTATCCACCATGTGTGGATGGTGAGTTCACTTGCGCCAATGGACGGTGTATT CCACAGGCTCAGGTGTGCAATGGTGTGAATGACTGCAAGGATAATGCCACATCGGATGAAACGCACGAACGGTGTCCCAT GAACACCACTTGTCCGGCGAATCATCTGAAGTGCGAGAAGACCAACATCTGCGTGGAACCCTATTGGTTGTGCGATGGCG ACAACGATTGTGGTGACAACTCCGACGAGGATCCACTGCATTGTGGCCAACGAACTTGTCCAACCAACAGTTTCCGGTGT CCCAACCACCGATGCATTCCAGCTACCTGGTACTGTGATGGTGACGATGACTGTGGCGATGGAGCCGATGAACCACCAGA TTACTGCAAATCGGAAGGACGCACATGCTTCGGGGATCTGTTCACCTGCGACAATGGCAACTGCATACCAAGGATCTACA TCTGCGATGGCGACAACGATTGTTTGGACAACAGTGACGAGGATAACCGGCACCAGTGCAATGACCGTAAGTGTGATGAG GAAACGGAGTTCACTTGTGTGGAGAACAAATCCTGGCAGCGTGCCCAGTGCATACCCAAAAAATGGATCTGCGATGGTGA TCCGGATTGCGTTGACGGAGCCGATGAGAATACTACTCTGCACAATTGTGCCACCCAGCAGCCCTGTGGCGAGGATATGT TCACCTGTGGCAATGGACGTTGCATCAATAAGGGATGGATCTGTGACCATGACAACGATTGCGGCGATGGTACCGATGAA GGCAAATTCTGTAACTCCAAGTACAAGACCTGTTCGGCCCAGGAGTTCACCTGCCAGAACTTCAAGTGCATCCGAAATCA ATCCCGGTGCGATGGCGAAGACGACTGCGGTGATCACTCGGATGAGGTGGGCTGTGCCAAGGAGAACATAACCTGTCCAC AGGGTCAGTTCGCCTGTACGAATGGTCAGTGCATCGACTACAATCTGGTGTGCAACAAGTATCCGGATTGTGCCGACGAG TCCGACGAACCTGCCCATTGCAACGTGGATGAGTGCGCCAAGGTGGAGATCAATCAGTGTGGCCACAAGTGCGTGGACAC GCTGACAGGTTACTACTGCGACTGCAATGAGGGCTACAAACTGCTAGCCGATGGCAAAGCCTGTGCGGATGTGGATGAGT GCCTAGAGCAGCCGGGCGCTTGTTCTCAGCACTGTTCCAATACCCCGGGTGGATTCTACTGCAAGTGCGACGAGACCTAC TACGAAAGGCAGAACGATGAGCACACGTGCAAGCGTAAGGACAAGATCCCACCGTGGCTGATCTTCACCAACAAGTACTA TGTGCGCAATATGTCGGTGGATGGACATCAGTACAATCTTATGCACCAGGATCTGATGAATGTGGTGGCTCTCGACTTCG ATATACGCGAGGAATACATGTACTTCTGTGATGTCACGGCCAAGACCATCTTCAGAGCGAAGTATGGAGAGGCCGATGAC GAGATGCCGCCGGAGAGGGAGGCTGTCATCAGGCACGATTCCCATGGCCTGGAGGGCATCGCCATCGATTGGGTGGGTCG CAAACTGTACTGGCTGGACAGGCACTCCAAGAACCTGGATGTCTCCGAATTGGACGGCAGCAAGCGCAAGACACTGAGAA GTGGCGTCGTCGATCCGCGTGCCATCGTCGTGCATCCTGGTATCGGTTACCTGTACTTCACCTCCTGGCATCTGCAAGCC TATATTGCCAAAATGGGCATGGATGGTTCGAACTTCTCGAGAATTCTAAACTGGAACGATGGCATCGCCTGGCCGAATGC TCTGTCCATTGATTACTTCACGGATCGAATTTACTGGGCAGATGCTCACTTGGACTACATAGCATATGCTGATCTGGAGG GCAGACATCGTCATACGGTGCTCTCAGGAAGCAAGGTGCCTCATGTGTTCGCACTGAGTCTCTTCGACGACTACATCTAC TGGAGTGACTGGAATTTAAAGGCGATCGTGAGGGCCAACAAGTTCCATGGTGCGAACTATACGGTGCTGAGGAATACCAC CCATCGACCGTATGACCTGCACATCAATCATCCGCTGAGACAGTTGCCCTACACCAATCCGTGTGGCACAAACAATGGCG GTTGCTCACATCTCTGCCTGATTGCTCCGCCGCCGGAATCCACCTATCTGAACATCGAGGGATATATCGAGGAGGGTGCA CCAATCTTCAAGTGTGCCTGTCCCAATCAATTCTATTTGGCCAGAGACATGAAGACCTGCGTGGCCAACTGTACGGCCGG ACAGCATTTGTGTGGCGGACGAGATGAGAAGTGCATCCCATGGTTCTGGAAGTGCGACGGTGAGAAGGACTGCAAGGATG GCTCCGATGAGCCCGCTACCTGCGCTCCTCGACACTGCCGTGCTGGAACGTTCCAGTGCAAGAACACCAACTGCACACCA TCGGCGACCATTTGCGATGGAGTGGATGACTGTGGCGATCGCAGCGATGAACAAAATTGCGATCTACCCTGTCCACTATC CGATTTCAAGTGCAAGTCCAGCGGCAGATGCATCCTCGATAGTTGGCGCTGCGATGGAGATGCCGACTGCAAGGATGGCA GCGATGAGGATCCAGCCGTCTGCTTCAAGCGAACATGTGATCCGAAAACCGAGTTCTCCTGCAAGAATGGCCGCTGCATT CCGCAATTGTGGATGTGCGATTTCGACAACGATTGTGGCGACGACTCCGATGAGCCGGCGTATATGTGCCGTCAAAGGAA CTGCACCACCGGCTGGCAGAGGTGTCCCGGCCAGTCCAACTATCGCTGCATTCCGAAGTGGCTGTTCTGCGATGGCAAGG ACGATTGTCGCGACAACAGCGATGAGCTGCCCGAGAACTGTCCCAAGTGCAATCCGGAAACGGACTTCAAGTGCGGCAAC AACCGATGCATACCCAAGCAATGGATGTGCGATTTCGCGGACGATTGTGGCGATGCCAGTGACGAGAATGAGGCAGTGTG CAAAGGACGCTATCGGGAGTGCTCCGAATCGGAGTTCCGTTGCGGCAATGGCAAGTGCATATCATCCCGCTGGCAGTGTG ACCACGAGGACGACTGTGGCGATAACTCGGACGAGATGCACTGCGAGGGATACCAGTGCAAGAATGGCACCTTCCAGTGC GCTTCCGGTCACTGTATTGCCTCCTACTTCCGGTGCGATGGCGATCGCGACTGCCGCGACATGTCCGATGAGGTGGGCTG TCCGCCCAGATTCCCCGGCGGTCGCTATTGTCCTGAATCGCGTTTCCAGTGCAACAACAACCTGTGCGTTTCGCTGTCCG ATTTGTGCGACGGCACCGATGATTGCGGCGATGGCAGTGACGAGGATCCCAGCGTTTGCAGTGACTTTAACTGCGATACC CTGCGACGATTCCAGTGCTCCAATGAGCGCTGCGTGGCCCGCTATCAGATCTGCGATGGCGTGGACAACTGCGGCGATGG CAGCGATGAGAACAACATGACCCTATGTGCCAGCAAACAGAAGCCCTGCGATCTGTACACGCAGTACCAGTGTGCCAACA AGCATTGCATCGAGCGGTCCCAGGTGTGTGATTTCTCCGACGATTGTGGCGATGCTAGTGATGAGTTAGGATGCCACCAC ACGAGCAGTTGCTCCGAAGCGAATCGGGGTGGATGCCAGCAGCATTGCCACAATCTAACGGATGGTGGATACATTTGCAC CTGCTATCCGGGCTACATCATAGCCGCCGACAACAAGAAGAAGTGCTCCGACGTGGACGAATGCCTGACGCGACAGCACA CGTGCTCCCACCAATGCCACAATCTGAATGGCACCTACTCGTGCAGTTGTCGCGAAGGATTCCATCTGACGGACGGCGCC AGCGGCGTATGCCGTGCCGAAAAGGAGGATGTCATTCTCCTGTTTGTTAATGGTCAGGAGATCAGAGGTCTGAATTGGCA TAAGAGCGAGGAGTTCGCTGTGATAGCGGCGGAGAAGAGGATCGAGGCACTGGACTACGATGCGCAGCAACAGATCGTCT TCTGGGCGGATAGCTATGACAAGACCATCAAGCGATCCTACATGGTCAATGCCATCGATGGCAGGGCCAAGATCGGATTC GCCCAGGACCTGAACATGAAGGGCGGCTCGAAGCCCACTGCTGTGGCTGTGGACTGGCTGGCCTCGAATCTCTACTGGAC CGAAATGGACAGGACGGGCTCGAAGCCGCGTGGACGTGTCATGGTGGCCAAGACCGATGGTCGCTATCGTCGCTCGATCG TGAATGCTGGACTCGAGGTACCCACCTCGATTGCTGTGAATCCGCAGCTGGGCAGAATATACTGGTCGGATGCGGGATCA GCTCCCAAAATCGAGGTATCCTGGATGGATGGCTCTAAGCGCCGCCCACTGATCACCGAGATGATACGACATCCCGCCGG CCTGACCATCGACTACTCGCAGGATCACATCATATACTGGGTGGACACCAAGCTGAATGCCATCGAATCGATGAGAGCCG ACGGCTCGCGCCGAAAGGCCATTGTGCGGGGCGATCAACTGAGGCATCCGGTGTCTCTGGATCTCTTCGAGTCGAACATG TTCTGGATGACCCGCGATACGGGTGAGCTGGTGCGCCAGGATAAGTTCGGGCGCGGAGTCCAGGTGGTGCTGCATCGCTA TATCGTCAATCCGTCCGGCCTGAAGGTGTACCACGACAAGCGGTACAACACCTCGCTGCCCAATCCGTGCGACAACTCCA CCTGCTCCCACCTGTGCCTGCTGGTGCCGGGCGGCCATCGTTGTGCCTGTCCAGACGCCTCTGGACCGCCGCCCTCGCAC CGCAGCACCGCCGAGGTCATCTGCAATGCTGCCGCCGAGCATCCGCGCCCGGCTCCGCGAATCTGCCCCTGCCAGAATGG TGGACTCTGCAAGGAGGACGCCCAGGGTGAACTGCTGTGCGAGTGCCGAACCCAGTTCGTTGGCGAGCACTGCGAGACAA GCACGATGGGAGCCTTTGGCCATGGTGACGCTAATGTCACCGCCGTCGTGGTGCCCATCATGGTCATCCTGCTGGTGATG ATGGCCGCCGCTGGCGCCTGGTATGTCATCCGCAAGCGACCATTTGGCAAGCTGGCTCGCATGCCGGCGATGACCTCGTC GCAGAGCGTGACCTTCCGTCACGGTTCGAATGTGGAGTTCAACGAGAGCGGCTTCCCAGGAGCATCTGCGCCGGGAGCTG GCGATGTGGCGCCCATCGAGGGCTACAACCTGCAGACGGTGAACGCGAACAAGGCGCGCGACTTTGCCAATCCCATGTAC GATGCAGTCCAATCGGGCACCACCGCCGATCCGGGCATGGGCAATGGTTCGGGCATTTATGATGTGCCCGGCGAGCCGTC GGCCAAGGTCAAGTCCATGGGCCACCATGCGGGCGGTTCGTTCACGGAACCCGCCTCGGCGATCATCGCGCCCAGCAGCA TTACGCACAAGGCGTCGCCGCAGCTGCAGCTGCGCACCAGGGAGCTAGATCCTTCGGCGGACACCGGCAAGGACACGCAG TTCCTGGTGGAGGAGGATAAGTCCGAGTGCTGATATCAAGCCCGTCAATCGGCTGTCGTTGCGGCGGCAGTTGCGATGGC CAGTTGCCGCCAACAGCGCCGGCAGCATCAGCAGCAGCAGCATCCCGAGAACAGCTATCCGCTGCAGACATAACACATAC TGGTCAAGCAGAGGAGGGAGCAGCATCAGAAGCAGCAGCATCAGCAACAGCAGCAACATCAGCAGCAGGGACAGGGACGT TCTAGTAGTAAACATCACAAATCCTGGCAGGTAGCCACCGCTGGCAAGGTCACCACCAAGGTCTAGCCGTGACCCCAACA GAAAACACACACCACCCACAGAGAGTAGCAACACGATCAGGAGGAGAAGATGCAGGAGGAGGAGGAGAAGGATCAGCAGA AGGAGGAGTAGCAAGTCCTCGGGGACTGGGGACGCCAATAAGCAAACATATCCAAAAATTGATAAACATATGCAACCCCC CACAGGAGAAACCAACTAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
