CTTGACTTGCCAGACTCTCCAGGCCGAGTGTGTGTGTGTGTAGTGGTTGTGCTAGCAGAGCTGTTGCAGAAACTAACTGG GAAAACACAAGATAGACGCGTGTGCGGCGAGCAGTAGCTAAATTGAACTGAAGTGAACAGAAAATATTTACACAAAATAA TAGAGATACAACTTCGGAAAAGCAAGCATACAACACACCGGGACTCCGCAATACGGGTTTTTTATTTCGTCTACTCTTCC GTACCTGGGCAGAGATTTAGATTTATCGCCTCCTCCACCCACTACTTCATCATCGACATCACCAGTGCTGTTGTTGTTGC TGTCGTTCCGCCTTCGCTCTCACGTAATCCGCCCCCGCACCACCTCGCCCGTCGGCCAACCGGCCCACAAACTAACTGAC TGATAGACCAAAAATCGATTAGCGGACTCTCTTCCTCTTCAACATCTTCGGCATTCGTGTGTGCGTTGCTTTGCGTTACG TTACGGTTACCGTTACGTCGCCCGCGCGTGTGTGTTGGTGTGTGAGCAAGGTGTGGTGTGTATAGAAAATCGATTGGCAA TTTGAACCGAAGAGCGCTATCGTCGCCGCAGTCGCCGCGTGTGTGCCGTTACGCGATTTTCAACAGCAGCCGCGCAGTAA TATTGCTTTTATTTTTTTGCCTGTGAGTCCTCCTAAGTGAGTGCGAAAGAGAGAGAGCGAGAGTGAGCTCTCTAAAACCT CTGCAGAAAATAAAAAGGAAAAGAAAAACACCGGACATGGATAAATCAGCGGCCACGAACTCGGCCACTGCCGATGGCCA AAACGAGGCAGCAGCAGCAGCCGCCGCCGCAGCAACAGCAGCTGGGTGTGGCAGCAACAACAGCAGCAGCACCAGCAGCA GCAACAACACTGCAAACAGCAATAATGCACTGCAGCGTTTGGCAACAACAACAAGTGCCGTTGTGGCATCCCCGATGACG ATCAACCTGAACATCAGCACCACCACCGGCGGCAATTTCGGCGTCAGCGTCGAGCCGCACATCAGTGTCGAGAGTCTGAA GAAGATCATCGCCAAGAAGCTGAAGGTGGCCAAGGATCGCATCTGTCTGCTGCACCGGGAAAAAGAACTGCAGGATGGCA CGCTCAGAGATCACAACTTAATGGACGGTTCCAAGATCATTTTGATACCAAACGTGGAAACTGGTTTATTGGCTCAACGT CCGGAGAACACGGTTATGCAAGCGCTGGAATCGCTTAACGACGCACAGGTTAATGATTTTCTCAGCGGCAAGACACCGCT CAACTTGAGCATGCGCCTCGGCGACCACATGATGCTCATCCAGCTGCAGCTGAGCACGGTTAATCCGGCGGGCGGCGGTG CTGCCACAGTGGCCCCAGTCGCCGGTGGTGCGAGTGGCTCAAGCATCAACGCAGCGGCAAGCACAACATCAACGGTGCCT GTAATGCCAGCTGGGACTGGCTGTTCCGGCGCCAACTCCACATCATTGTCTTCGTCGGCGGTGCTGGCGGCGGCGGCCGC TGGAGTAGGCGGCAGCACTGGAACCAGTGGCTCCGGCACTTCCTCATCCTCATCCTCAACGTCATCGTCATCCTCTTCGT CTTCGTCTTCGTCGAGAACCCGCTCTTCTGGCCAGCGATCGAGTGGAAGAATCGGACATGGCCACGTGCACAGCCATCAG CATCCGAGTCTGCATGCTGCGAACTGGCATGGCTTTAGTCACGGACATGGACATGGTCACAGCCACGGAAACGGGCATGG ACATCATCATCATCACCATCACCACCACCATCATCACCATCATCATCACAACGCTTCGGCAGTGGCTGCGGGCAGCGGTG GTGGGATGTCCTCGCTGCGCAAGATGTACGGCGATTTGCCCAGCTCGTCTGGAGCATCTGGCTCTGCTCCAGCAACTGGC ACTGGTCAGTCCCAGAGCTCGTCAACGCTAAACGGACCCGATCTCACCAAGCTGCTAATTAAGGGCGGCATTCAGAATAT CGTGAACAGCTTCGCTCTGAAGGATGCCGGCGCCAATGCTCGTCCTGTGGCCAATGGAGGATCCGGATCGAGTCCAGGGA AGCCTGTGTTAGAGCAGTCGCCCATCAAGTCGCTGTCGAATCTCGTGTCCAGTCCCATCAAGATGACAACTATCAAAAAC ATTCCCCTGCCCACGACGGCTGCCACAAGTTCCAGTTCGAGCTGCAACTGCAGCTCTGCCGCCCCCAGCTCATCCTCGTC CACTTCCAGTGGAGCCTGCGCAACCGGTGGCTGCCAGCAGGATCCGATAGCGGCCAAGCTGACCTCGTGCTTGTGCACGC GACTACCCACACCGTCAGTGGCTCCACCGGTGGCCACTTTGGTGGCGCCTGCTCCGGTGGCCAGATCCGGATCTACCAGC TCACCTAGCAAGTGTCCCGAATCGAGTTGCAGTGGAGCGAATGCCGGATTTGCAGCTCGTTCGACTCTTGCGGGCACAAG AGTCACCTTCTCTGGCAATTCGATGCTGCACAAGACGGGCAATAATCGCATCACTAGAACCAAGCATCGTCACTACCACG GGCAGGGCCACGGTCATGGACATGGAAATGGTCACAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCCACTTT TTTAATCACGCCTGCGCCGGCAACCAGCCATCGGTTAACGCAGCCGCCTTTGCTACAAGCTCATCATCGTCTTCCTCGTC CCCGTCATCTTCCTCCTCTTCCCCCTCGAAGCGTCGCAAGCTGGAGCAGGGAATGGGAGCAGGAGCGGTAGGAGCAACGG CCGCAACCGCAACAGCAGCGGCAACGGTGGCACTACCAGCCAGCACCAGCGCCACAACCGCAGAGCCCGATGACTCGCTG CTGGGTGTGTCTGATACTAGGACTCTCGCTGAGGCATCGAGAAATCTTACCCAGACATTGCGTAAACTCTCCAAGGAGGT GTTCACCAACAAGATCGATTTATCCGGAGCCGGCGTGGTGAGCAGCGGTCGCAGCTCAAGCAGCGGTGCCACCTCGAGCG GAGCAGCCAGTGGTGAGGAAGCACCGCGCAAGAGCGGATCGGGAGCCGTGATCGAGTCTATGAAGAACCATGGGAAGGGG ATCTACTCTGGCACCTTTTCGGGCACACTCAATCCGGCACTACAGGATCGCTTTGGACGACCCAAACGCGACATCTCAAC GGTGATCCATATTCTCAACGATCTGTTGAGTGCCACACCGCAATACGGACGTGGTGCACGCATTAGCTTCGAGGCCCCGA CCACTGGATCGGTTTCGGGATCTGGTTCGGGATCCGGCAGCAGTGGATCTTCCTCGACTACTTCCTCTGGTAGCGGGCTG CATCACGGATCGCGTAGCAAAATGTACTCCTCCAAGCACCACAACTGCGCCAAGTGCAACAGCCGCGCCCAGCAGCAACA ACAGCAGTCGGCGACTCTGGCCTCTGGTAGCGGAGGTAGCTGCTCTTTCCGTGACTGCCCGAGCTACCACTCCTCCTCCG CTGCCACCAAGGCTTCCACCAGCTCGAAGTCATCGTCGCATAGTTGCTGCCAGACTGCTGAGGCACCCAGCTCTATGACC AGTCAATCCAATGGATGCACATGTCGCTACCGCCGCGATGATGGACAGGGCGAACGGGAGCGTGAGCACACATGCCAGAA GTGCACCGTGGAGCTGGCCAACATGAAGACCCGCTCAAAAATGGATCAGCTGCGCTTGGTGATGCAGCAGCACAAGCAGA AGCGGGAGGCGCGTAAACTGAAAAGTGGTCCCTATGCCACAGCCGCCTCCGCATCCGCGGCGGACAGTCTCTCCTCCATC GCCGCTGGTGGAACAGCTGCTGTTCCGATGGGCGCGGCCGCACAGTACAGCGCCGTGAGCGCCCTGGTGGCTACGCCCCT GCAACCAGCCGCTGTGCCCGTTCAGACGGGGAAAGCTGCACCCGCGAACAGCATCACTGGCAACAGCAGTAACGCCAATG TCAACGGCAATACTTCAACGGCACCTGCAACAGCAGCAACGTCAGCAGCAGCAGCACCAACAGCGGCGCCGCCCAGCGAA GTGTCCCCGAACCACATTGTGGAGGAGGTGGATACGGCGGCCTAAAGGCAGCAGCACCATCAGCTAGAGCCACCACCAGC CCCGCTGACTTTATCCTATTTTGTAGCATTTACTAGTTTACGCAGCAGCAACAAAGAACGAAGAGCAAACAAAAGATGAA GATGATGATGATGCGCAGGATCCAGTAGAAGCAGATGATGGGCAAACAAAAGAAACTAGAACCACAACTAAAGCTTAAAT TAATCTAGAGTTAGTGCATAACAACACCAATTCTTCTGTTCGAACACCCGCTCGTTTGGGTTTCCCACCTCCACGATGGG GCAAAATGGAAATGGAAGCGCCCGGAAATTCGATAAGCTGTTAAATTAATTTTAAGTCCCTAAAACACACCCACTTCGGT GCAAGCCAATATTTCGTTGGGAGCAAATGAAGTGAATGAACCAGCTTCTGGAGGCGAAATCCTTTAATGTTTTTGTAAAT TAAATTTAGTGAAATTTTTTGTGTACATCCCAGGCGCACGCTATGCCGTGCAATTCACAATTTGAATCGTTAGCAACCAG CAGCGTGAGCGGAACGGAAATAGAAATGTATGAAAAAGGAAAAGAAATTCTTATAATGGAAATGATTAATATAGAACTCA ATGTAAAACACACACACAGACTATGCTGAATGATTTGTAAGCCAAAGCGGTGGCAAATTTTGGTAAACTAAAATGGAATT TAGATTTAATTTTAGATACACCAAATCAAATCTAATACAACACACACGATCACACACACACACACCTGGCTTTGGCAGCT TTATGTTATGTTTAACTTTAATAACCGACGGCGGGCTGAATGAAAATCTGCACCAGCGACTGCGATGTGGCGCTTTAACC TAATTTGCCTTAATAGAAAATCGTTTTTCACCTATTTTTTTTTATATATACTTTATCACCAAGAATGTGTTTCGAAACGC GCCGGTAAAGACGAGCGACGAATTGAATTTTGTTCACCTACACCTATACTATTGAATTAGTTAGTAAGACTATGTTTTCT ATTCATTTTGCAATTATCATTACACTTATAATTACCAATTGGATTAGTTAAGTTGCGCAATGGAAGCGTCTGAGAAGCAG AAAGTAACGCAAACGTAGTTGTATAGTTTTAATTTAAGCCCAAGCAATTACTTTTTTTGTCCTACACTTACTATTATAAC CCAATTCAACAAATGCAATTAGATCTAGGCGAGTTTAACTTTTAAACTTTTGAGTTGGTTTCATTTGTCTTTGCCTCGTG TGCACCACAACTACTTGATTTCAATAAATGTTTAGTAAATGGTTTAGTTAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
