FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\GH09538
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General Information
Symbol
Dmel\GH09538
Species
D. melanogaster
Name
GH09538
FlyBase ID
FBcl0102927
Feature type
cDNA_clone
Computed gene(s)
Dmel\TrpRS-m
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • reverse transcriptase error
  • Caution: This cDNA clone replaced by FI03887
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,223
    NCBI
    AY070507
    Full Length sequence dataSequence Downloader
    CAACGCTCGCGAACGCTCAGTGTCAAAATTTCGGCCGTTTCGAACAAATAATTTATTTTTGGGCTTTGTTCACACATTTA
GCACCGCATTATTCCCAATATTTTTTACTCAAAAGTTTTGGGGTACAAAATTCATAGCTCATTGCAATGTTCCGATTTGG
CAAGCTCCATCGCAGTCTGTCTAAGGTGGCCAAAAAGGGACAGACAGCTGTGTATTCCCAGCGCCGTTGCATTCAGGCGA
GGAGTTTCTTGCCGGGATTGGCTAGTAGGACGGCTGGAACGCCCTCTGCGACGGCCCAGGTAACCGGTGGAAACAAGCAG
CACGTACACCCCCATGGTAATTCCGTTAACAGTGGCCACGAAGAACATAACACCCGCTGGCCGAGGAAAGTCTTTAGTGG
CATCCAGCCGACGGGTTCCCTGCATCTGGGTAATTATCTCGGCGCCGTGCGCAAATGGGTGCAGCTGCAGAATGCTCGTG
ACGATGTCACCGTGTGTATTGTGGATTTGCACTCTATTACCATGCCCCACAATCCGCCGCTTCTGCGAGAGAACATCTTC
ACCATGGCCGCCACGTTGTTGGCATGTGGCATCGATCCCACCAAGAGCACCCTCTTTGTCCAGTCCGCCGTTGCCGAGCA
CGCCGAGTTCAATTGGATCCTTAGCTCCTTGACCACCATGCCGCGATTGGCTCAACTGCCGCAGTTCCGAGAGAAGTCCC
GTCTTCTAAAGGATGTCCCTCTGGGATTGTACGTTTACCCGGTTCTCCAAGCAGCCGACATTATGCTTTACAAGTCCACT
CATGTTCCTGTGGGCGCTGACCAGATTCAGCACATTCAGCTGGCCCAGCACTTGGCCAGAATTTATAACGGTCGCTATGG
CGAGACTTTCCCTGTTTGCACCGCCATCATTGAGGATGGTGATGCCTCCCGTGTGCTGTCCCTGCGAGATCCCTCCAAAA
AGATGTCTAAATCGGAAGCGAATCCCAAGGCCACCATCAACTTGTGTGACTCGCCCGACTTGATTACGCAGAAGATAAAG
AAAGCCGTCACAGACTTTACATCGGACATTACTTATAATCCTGGCAAGCGTGCTGGCGTCAGCAATCTGGTCAACATCCA
TGCCCAGGTCACGGGACAGAGCATTAAGACAGTGGTGAACGAGGCCGCCACTTTGGACACGGCAAAGTATAAGGACCGTG
TGGCGGAGGCTGTGGTCGAGCACTTGCGTCCGATCCGAGAACAGATACACCATCACATGACTAAGCGCAACGAGATGATC
TACTTGCTGGAAGTGGGTGCAGAAAAGGCACGTCAGCAGGCACGCCAAACGTTGAACGATGTTAAACAGCGCCTAGGACT
AGGAACCAGTGCCAACATTCCGGCTGCGGTGCATGTGGCTCCACTTCTGCCCGCACCCGATATTTCCAAAACCTCAGCAA
GCAGGCGAATGTCCAAGGACTTCGACGGGAATGTCCACGAGCGTCACGAACGGATGTACGGATTCGGAGATCAGCCAAGC
GGAGGAGCTGGTCAGTCAGCCACCGGCGAGGTGGCGGATATGCCTCAGGCCGTTGTACCGCGGGTGATCCGTCGGGCGCC
CATCGTTCCCACTCAGTCTATGCGCGTAGAGAAACAACTAGGTAGCACTCGAACTCGGCACGTATCCAGATGGACACCTG
CAATGCTCCGCATATAGGGTTCAAACCGCCGCCAGGATTTTCGGCCAGCATGGTCACCGGTGCTCTAGCCAAGAACAAGC
GCGCTACAGTTAAACCCGTTACTGGCAACCTGGGCTTCGGCCAGCAATCGCAGGGTGGCTCTTACAAGCACAGCCAGAAC
GTGAATCCTAGTGCGGTATCCACCATTGCACGTGAAGTAAACGCTGCTAAGAAGCAGGAGTTTACCTGCAACCCCGCTTT
GTACGGCTCTGCCTTAGAATCGCTTTCCAATTCAAGTCGCTCGAGCGAAAAGACTAACTCTACGGCAGGAGACAGTGAGT
TCATAATTGTCGCGAGCGAGGAGGAAGAGCAGGCTGCTTCCTCTGAGGAGCAACGCGAAGAAGCTGAAGAGGAGGAGTCC
GGGGAACGTGAAAGGGAGAAAGGCGAGCGCAACGCCACTGAAGCTACCAAAGTCGAGATTTAACTCTCGGAAACATTGCA
ATTGTTATAAGTGTAGTACAAGTATACTTAGGTAACATTACTGTTAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)