FB2026_02 , released June 18, 2026
Clone: Dmel\GH28326
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General Information
Symbol
Dmel\GH28326
Species
D. melanogaster
Name
GH28326
FlyBase ID
FBcl0108005
Feature type
cDNA_clone
Computed gene(s)
Dmel\Nt5b
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      2,491
      NCBI
      BT120037
      Full Length sequence dataSequence Downloader
      ATTTTGCCATCCAAGTGGAAAATCCGAATTCGCATCCGAATAGCAATCGTAGATACGAAATCCCAAATTGTCAGTTTTTT
TTTTTTTTTGTTTTTCTCACTCGAAGAGTTTTCCAAACGATCGATCAGTCAGATTCATTCCAACTCCAGATCTTGGTGAC
GTCGTCGTCCGCCAAAATAACAGCTGATAAGATACTTATCGCTGCTGGTAGCCCCCACAACCACCCCGCGCGCCTACCGA
CGTTCCACGCCCACTGGGCGAAAGAGCTTAATCAGCAGCAGCGACGCGGCGCAGCATTTGTGGCCTTGTAATCCGCGGCA
GCTCCAAGATGCCACAGCCAAAGCAAAACCAGCAGAAACAGCCACAGCCACAACAGCAAAAGCCGCTGCAGAAACATTCC
AGCACCAGCTCCACGATCATCGCCAAGCCGGAAAAGGAACGGGAACGCACGCAGACGATCGGCGAAACCGACATGGATCA
GTCGGTGCTCAATGGACTGGCAGTGGGCGCAGATGGAACCGCCGGCTACCATGGTCACAAGCGAGAACTTGGTCACAGAG
TGTTTGTGAACAGATCGCTGCATTTGGAGAATATCAAGTACTATGGCTTCGATATGGACTACACCTTGGCAGAGTACAAG
TCACCGCAGTATGAGCAGCTGGGCTTCAACCTGGTCAAGGAGCGCCTGGTCTTCATGGGCTACCCCAAGGAGATCCTGCA
GTTCGAGTACGATCCCACCTTCCCAGTACGCGGCCTGTGGTTCGACACTCTCTACGGGAATCTGCTGAAAGTGGATGCCT
ACGGCAACATCCTGGTCTGCGTTCATGGCTTCGAGTTCATCAAACATCACCAGGTGTACGAGTTGTATCCCAACAAGTTC
TTGAAACTGGACGAGTCGCGTGTCTATGTCCTGAATACTCTCTTCAATCTTCCTGAAACCTATCTCCTTGCCTGTCTCGT
CGACTTCCTAACCAACAGCAGCGACTTTGTGCGCGTTGAGCGCGGCATCAAAGCTGGCGATTTACTTTTTACCTACAAGT
CGATTTTCCAGGATGTGCGACGTGCCGTCGACTGGGTGCATTCCGATGGCGATCTGAAGCGTCGCACCACTCAGAATATG
GCTCACTATGTGAAGAAGGACGAGCGTTTGCCCACGGTCCTTTCGCGAATCCGGGAGTCGGGCGCCAAACTCTTTATGCT
GACCAACAGCGACTATACGTACACCAACGAGATCATGACCTACCTGTTTGATTTCCCCCATGGCGCCACTCCGGATGAGC
CGCATCGCGACTGGAAGACCTACTTCGATGTGATCGTGGTGGATGCCCGCAAGCCGCTCTTCTTCGACGAGGGCACCGTG
CTGCGGCAGGTGGACACGAAGACCGGAAGCCTGAAGATCGGCACACACGTTGGACCCCTGCAGCCGGGTCAGGTTTATTC
CGGTGGCTCCTGCGAGCTCTTTACCAAGTTCATCAATGCCAAAGGCAAGGACGTGCTCTATGTGGGCGATCACATTTTTG
GCGATATTCTCAAATCGAAGAAGATTCGAGGCTGGCGCACCTTCCTAATTGTGCCCGAATTGGTGAGGGAGCTGCACGTT
TGGACGGATGAGTGCCAGCTGTTTGCGGAGCTACAGAACCTGGACATCAAGCTGGGCGACCTGTACAGGGATCTCGACTC
GAGTGCCAAGGTCAAGCCGGACATTTCGCAGCTGCGCACGTGCATCCGCGATGTGACACACAAGATGGACATGTCCTACG
GTATGATGGGCTCACTCTTCCGCTCCGGCTCGCGCCAGACCTTCTTCTCCAGCCAGGTGGTGCGATATGCCGACGTCTAT
GCGGCCACCCTGCTCAACCTCATTTACTACCCATTCTCGTATATGTTCCGAGCTCCGGCCATGCTTCTGCCCCATGAGTC
GACGGTGGCCCATGATCAGGCCCATCAGCCGCCTCCGCCACTGGGCCCTGTTCCTGTTCCGGCTACGGGAGCCGCATCGG
TGGCTGCCTCGCCGGATGAGCCGGCTCGCATTTTGGCGGGCACCGCCGAGCACGTTAAGGACCCCAAGGACTTGCATCGC
GCAAGCTCCATTGTGCACACTCGTCCGTCGACGCCCACCGTGGTGACCCACACCCATGACGAGGACTATTCCGAGGAGGA
GGAGACACCCAGTGGTGCCGAGTCTTCCACAGAGGCTGTGCGCGATGCCTCCGAAGACTAGATGATGCCACAGCCGCCCT
TCTGGAATGGAATGTTTGGATCAACGAATTTTATTCTCAAACTTCCCCAATGCCCCCATTAGCCTTGCAAACCCTTACCC
GAAATCCACACCGTGGTCGAAGCAATTATGAAACGTACGATTTTCCCTGGCGATTTTACCCAAGCACAGATATGTGAAAC
TATATACCATAACCGATATATATATATATACATTATACATTATACATTATAAATTAGCTGGTTCCATACATATAAAAAAA
AAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)