FB2026_03 , released September 17, 2026
Clone: Dmel\GH22222
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General Information
Symbol
Dmel\GH22222
Species
D. melanogaster
Name
GH22222
FlyBase ID
FBcl0110027
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG7565
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI19480
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,727
    NCBI
    BT010098
    Full Length sequence dataSequence Downloader
    AGCTGGAGTAATTGGTTATTTTTTTTGTTGTGCGCTGGCTGAAAGAAAAAATCGACGAGCCAGTGTTTTGCTGGTGATTT
AAACTCCGAAACACCAGCAAATAGTCGGTTACTTGCGGCGGAACCTACGGTAAAGATGGTTCAAGTGGGCAAACGGATTT
GCAACCTGCTTTTGCTGGCCACTGCGATGTCGTCAGCATATGCCGACGTCACAACGCAAAACGCTCTGTTGGTTGGAAGT
AAGAAACACAAGGAGACCAGCCCCGATAATTCAGTTGGCGGTTCTATATCGCCCAATTTGGTGTGCCACAAGATGCTGAG
GCATGTGTTCGAAAACGCCACTCCGCGGGATGAGCAGCAGGCGGGTGTGTTTGAGGAGTACAAGCCACCGCCGGATGCAG
TGGAGCCACTGGAGGAGGAGGCCTATCTTTGGAACTGCCTACAGGCTTGCTGCGAAAAGCCCCGGAATGGCAGCAGTGCC
TGCAATGTGGTCCTCGTTTTTAAGGCCAAATGCTACCATATCCGATGCCAGAGCAACGAGGCCTGCTTGCCCAAGCTCAG
AGTTCGGATGCCCAACGAAAAGGTTCAGATGGTACTGGTCAATCCACTGGGCGATGCCACATGGCCACAGCTCCTCAAGG
CGGAGGCAGCCAAACAGAATGCTGAGATTCTGCCATACGATGAAGCGGCACTTAATTTCTGGAAGCAGCCCAGAAGGTTG
AGCTACCTGGCTCGCAATCAGGAAACCCCCGTTTATGAGGACGAAGATTTCCCATTGGCCGACAAGCGAATGAATCAAAT
GATCTTCCAACCCGACGAAAACGATGTTCTGGCCAACGAAGAGCTCGGCTACTACGATTCGAATGCCAAGTTTACCACCT
GCGATATGGAGACACCTTGTCCACCACCGCAACAATGTGTACCACTACAGCCAAATGCCGTGCGTGGAGTGTGCACCTGT
CCAGAAGGCTTTGTGTGGAATAAACAAAGGAAGTGCGTAATGGCAGCGGTTCCCTATAGCTCATATCTAACCAGCAATGA
GGCGGGACAGCAAGAAGCGGCTGCTAGTGAGAACTCACCGGAGGTATCAACGCCGCCTTTGAAGGCTGAGCAGAACAAGG
ACATTGTAGTCTCCGTAATGTCCAAGGAAGTTCGTTTGCCGGAACAGGAGGTGACCCTAGCGGCGTTTACGGTTCCCGAT
GAGCAAACCAGCGATACCAAGTACAAATATCTTTGGACACTTATATCGCAACCCAAGGGTCCTATGAATGGCACTATTTC
GGATCAGAGCAAGTCGAAGGTAAAACTATCGAATCTTTCGGAGGGACTGTATACTTTTAAGGTCACTGTAACTGGTGACA
ATGGAACCTTTGGCGAGGCAACAGCCAATGTTACAGTGCTTCCCGAGAATCGAATCAATCAGCCACCACAAGTCATCATC
TCGCCCAGGGAGCAGATCATCCGTCAGCCCACCACCAATGCCATACTTGATGGCAGCACCAGTACGGATGATGATAAGAT
CACCAATTGGCACTGGGAGGTGATTTCGGGACCGATTGGTTACCAACCAGTTTTGCCAGAGGTTAACACCCTCCAGTTGG
ATCTCACATCGCCGGGGAACTACACATTCAAGCTAACCGTAACCGATTCAAATAACGTGACCAATTCAACCACTGCCACG
ATAGCAGTTCTTAAGGAGACCGATTATGCTCCGGTGGCAAATGCCGGCGATGCCGTGATCTTGTATTTGCCCAACAATAA
TGTCACTCTGAATGGCACAGCCAGTTCGGATGATCACGAGATCGTTGCTTGGGAGTGGACCAAGGATGCTAGCGACGAGG
CCAAGGCTGTGGATATGCAGAATACAAGAACACCCTATGTTCAGCTGTCCAATTTGGAGGAGGGCATGTACACTTTTGTT
CTAAAAGTAACCGATGGCAGTGGGCAATCGAGTACAGCTAAGGTCCATGTGTTTGTGAAGCCTCCCACGAATTCTCCACC
AGTTGCTGAGGCAGGAAGTAATACGACTACTAGCTTGCCCATTAATTGGGTTCTTTTGAATGGCTCCGATTCGAAGGACG
ACATTGGCATCAAGAGTTATTTGTGGAAGCAGCTGAGCGGGCCCAATAATGCAGTCATTTTGAAGTCCAACTCATCAATT
GCCAATGCCACCTCTCTGACTCTGGGACTCTATGAGTTCGAATTAACCGTAGCTGAAAAATAATAACACCGCTACGGACA
CCACGTGGGTGAAGATAGTTCAAGAACGGAACGCAGCTCCAATAGCCAACGCCGGTGGTGATCACACCGTCACCCTGCCG
GCCACCGCCATCTATTTCAATGGCTCTAAATCCTGGGATGACCTGGCTGTGGTCAAGTATCTCTGGACACGCGACGAGCA
CAGTTTGGCAGCGGGCGTCATTGTGGCAGATACCGACAAGGAGCCCGTAATGATTTTGACCAATTTAGTACAAGGCCGCT
ATGTATTCACACTGACTGTGAGCGATGATCAGGGTTTGACCAGTTCAGATACTGTCAGCGTTAATGTCCGTCGCGATCCC
AAGCTATTGAATTTGGTTCAAATGACCCTGCCCATGGGCATCTCTGTGCTCGTACAATCCGAACTGGATTCGGTGGTGCA
AAAACTGCAGTTGTTGCTGGGAGATGAGAATAAGATCCAGGTCAGGGAGCTAAAATATGACTTGCATACGGATGCTACTG
TCCTGGTCTTCTACGTAAACGATGGACAGGGTAAGGCATTGGATGGTTTGCAAGTGGAGCGGCAGTTGAGAACCCAACTG
CAGAAGGATGCATCTATACTGGGCGCATTTGCAGTGGATATTCGCACCTCTGTTTGCCAGAGTGATTGCTCCGGTCATGG
GTCGTGTAATCCCATTACCAGAGCTTGCATATGCGAGGCGTTCTGGATGCCATCGGCTGGCTACTTCTTTAATAACCAGG
AGGCCAATTGCGACTGGTCCATATTATACGTGTTTGTTGGTGTGATTGTGGGCTGCCTCTTGCTATCTGGAGTATTTTGG
GGCATAGCCTGTGCCTGCAGACAATCGAAAAAGCCGCGTCTTCGTCAGAAAGTGCAAAAATATTCATTGATTGGTAATAA
GGATGAAGAAGCAGCCAATTATTCTCGAAACACATCGCTGACGGAATCAGAGACGGATTCCGATGTCCTTTTTGAGACCC
GCACCAAGTCCAATGGTCTGGGCAAGCACAAGTCGCACAATTCTCACAGTCATGGACATGGAAGTAGTGGAGGCAGTGGA
TCTTCCGGACGGGATGGTCATAAATACGGAACACAAAAACTGGGCCGCAAAATCAAGGCATAAAAGTATATGCATCAGAA
GGATAAGGAGATCAAATATGTGTGTGTGTTAGTCGATCTACTATACCGTGTGTGTGATATCTATTTGTATTTCTATAATG
TACTCGGATCTTTTGTACTTTCCGCAAAACTCGTCTGAAGTCAAGTTGTCTATAAGTTCCTTTACGTGCAATTATTTTAA
GTTAAGAGTCATTCTTTATATTATTTTAAGCTGCTGCTGTGCAACAAGTATTATATTTAATGTTTATTTCTACTGCGATA
CTTTTTATGTGTGTATATAGACATAGCTGTTCACAATCAATGTAACAAACGACATGTAACTGCTTAAAAGTGCATTATTA
CCCACATTAAACGAAACTAATAGATGAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)