- Tools
- Downloads
- Links
- Community
- About
- Help
FB2025_04
,
released October 2, 2025
TTGTCTTTGCGAGTTAGCCATTTGCAATCGTGCGGTCTTTTCAATGTGTCTGTAGCCGCCCCAATTACATATGTATTTCG TCTGTGGATATATGTACGATGTACATATGTATATAGGATGTACACATTTGTACATATAAATATATGAACAATGCCTATTT CTCCAGCTTTCGTTTACAGTAGTTTTGAGAAAGAAGTGTATCTGAGTGTTTACGTGAATATTGAGGGCACAAAACAAAAT AGCAGAGCAAAGAAAAAATAGCTCGTTGTAAATTAGACATGGAGAACATACAATGCCTTTCAAAAAGTTATAAAAGTGAC CAGAAGACCGCGGAGATTTCGTTGTCGCGCTTGTCATATTGCCCAACCGGTCAGGACTCTTTCTACTGAACATTCTATTG TTATTTTATATAAGTATCTATGTACAGATGTATGTATGTTGAAGCCGAATAAAAAATTAAGCATGGATTTGTATATAAAT AAAAATGTTTACTGCATTCCCGACATATCAACAACACAATACATGTAAATGTATGTGTATGTACATGCATATGATCACAT TTGCTACTAGAAAGCTCTTAGAGACCTCACCGCTTTCTATTTGAAAATACATTTACACAGTTACACAAAAAAGATATGTT TAATACGGCCATATAAAATACTGCCATCCACTGCGAAGTACATAGTACAATTTTGACTAAAACGATTCCTTTCTATGGCA GCTACGTATAACATAAAGTCAGCGATCCGCAAACAAGATGAGATGAAAACGCAATGATTAAATCAAATTTGCATTTGCCA ATTAAATACGACAGCGGAAGTGCCGATAGTGCCAATTAGCATTATTGCCACGACGCCGATCTATGGTCACAATTTGTTCC TTGTCAGCTGCACTAAGTTGTAACATTGCTTATAGATTTCCTATCTATGAAATGGAGCATTTAGATCTTGCCGGCTATCT TGGCCACACGCCTCAAAGTTGGAATACCCTGGGAACCTTTCAGGATCAAGATGCTGTTTACCTATCTAGCTCGCGACAAA TGTTGGAACCAATTTTGGAGGAGACTAGCGAGGATGAGGAACGTGCCCGAAGTAGCTTGATTGTAAGCCTAGATCGCCGC TCCACCCTCTGGAGCTCTACGGAGTCTGAAACTGGATCAGTCTTACGCATAGAAACGATTAAGGATATCGATGCTGTTTC TGAGAGGGACTTTGTCTGCCCACCCAAGCGACCAAGGCGCAGCCATGACTTAAATTCTGGCCAGATTTCAGAGGATTGCG TGCAACTTCGCCGCCCTATGCCTTACGATCCCGAGTACCACAATAGCTTTGAACGCTTTCTGGCTTTGGAGGCATGTGCT CGCGATAGCTTCGGAAGCCAGGGCCAACGCAGCAGTATAGGCAGTAGGCGAGGGGGCACCTTCGAGGACTTTTATTCGGA CAGGGACTCCTCCCACTCGCTATCGCGCAGCTCCTCCTTGGTACAGTTCGAGTCCTTGGAACGCCAGCTCACGCTCCAGG AGCAGCATCAGAGTATGAACAGTCTGGGCAACAGTTCGCCCTCGCTCACCTGCGAGTCTGGTGCTTCAACGCGGGCCAGG ACCAGGGACAGCAGTCCAGAGAGTAGGAAGGGATCAGCCCCGACGCTGTCCCTGCTAAAGCGTTATGAATCCAGCGACTG TAGTCATCTGCACCAGACATACTATGAGCTGAACAATCTCAATTTTGAATATAAGCAGCAGCAGGAACTTCTCTCCAGAA GCCTGCGAGAGGCGGAGCTAAAATCTGACCTAGAGAGCTCCTCTAGCTCCAGCTCGTCCAGCGATAGCAGTGGTCACAGC CTTCTAAGCTCTTGCCTTGTTCCCGGAGATGCAGTAGCAACCGAAAGGGGGAAACAGGACGGGACGAATTCTCGTCGAAT TCCCCCCAACTCAGCTGAGAACCTGTCCGAGGACTCCGGCTACTGCGAGCCAAGAACCCTAAAGCGGGAAAAGTCCAAAT CCATACCAAAGAATTTCGAGAAACTTTGCGAAGAGGAGGAGGAGCTGCTGTTACTGGAGGAGGGCGCTCCGCATTCTCGC GACTTCAAAGACGAGCATGATTTGTGTCAGACCGAAATAGAGCAGACTGCGGATACGGCTGCATTAGCATGCTCTCCGTC GTCTATAAATAGTTCCCCGGAGAGGGCCAGCTCCGAATCGGAATCGGCATCGCCGCTGCCATCGCCGACCGGATCGGCAT CAACGTCGTCTGCCGCCTCGTTCACTTGGCTACACAACAGTTTGCCAGATATCCGCGAGCCACGAGGTCCACTCTCTGTG GAAATTAACAATTCAAAGCCCAAGACAAACGGCAGCGTTCTGGCCACCACCAGACAAAATAGTGGCAGCACCCGGAGCAG CAGCTCCGCCGATAGCTGTGACGGTATATCGCTCCTCGGCCCCACCCATTCACTCAACGAGCTCCAGGTGCTTGAACGGA GGCGACGACCACGTCATATTCGCCCCAGCCAGAGAAACTGTCACAGCAACAGTTCCAGTGAGGAACTGGAGGAGCTGGAC AACTTATTTGTTGCCTGCCACACCCCGCCAAGGATCCGACGCAGTTGCAGCTGGAACGAACGAGCTAGAAAAGTAGTGGG TGTGGCAAGTGCCGGATATTTGAACGCTAGTTACCAAAACCTAACTCTATTGAACTACACGGATAACTGGCTGCCGGCAG AGATTCTTCAGAAGAACCACAAGAGGAGCCCAGCAGAAGAAATGTCGAAACAAAGCAGTAATACCTGCGGAAATTACGAG CACATGATATGTAAGGGAAACTTTTTGCTGGATGAGATCAGCAAGGTTTATGACAAAAATGTGTCTATACTGACGGATAA GCCGGTGCTGGATACCAAGCTCCCTGCGCCAGTGGAATGTGATTTAAATGAACCTATTGAATGTTCGAAGGTGCAGCACG TGCAGCTAAACATAAAGCCACCGCCGCGCCAGAAACGTTTGACTCAACAGCCGCCAGCCGCCATGACCTTTAATGGCCTT GTTAAAACATTTGACCAGGATCCCACTAACTTGCGAACCTCGTACACCCAAAGCCTAGAGCAGTGCCACCTTGACGAGCA AGAAACGGCAAAGCCTGCTTCGTACCCGCCGTCAGCCATCTCACAACGGTTCCTGCCCAAGCGGCGATTCCAGTCCCAGA CGTCGGCGGCTAAGCAGCGAAGTCTGGTAAGCAGCACGCCAAATCTCGCGGCCAGCGACGACGGGCAGCCGGAATCCGAG TACGATATCTACGTGAGCAGTGCACACGCCTCAATGCACCAGTTGCCGCAGACAACAACGCTCCCGAAGCCGCTGGGGAT TCTGCTTCCAGCCGGATCGCGCCATTCTTTTGGCAAGGAGGTAAGCTTCTGCCCAGTGGTAAGCAAGTACTGTTGGCAGG AGCAGTCCTCCGAGGAGCCCACCGAGGACCAGTTCCACTCCAGCGAGGGCGAGCAGGGGCCGCCCAGCGATGACGAACTG ATGGACCCCACATTGGTGCACAACACCAGAGAGAACGAAAACATCGCTGCGCTCTTCGAGCCCGAGCCAGAGCAGGCGCA TCGTCTAAGAGAGCCGGCGATCGCACAAAAGTTTACCGGTTTCCATACCAATGCGCTCTTGTCCGCCTCCACACACGCAG AGAAAAACTTATCATGTGAAGAGAGCGCCAATGTCAAAAACAAAGCAAACTTAGCCACACCCGATTGCCGCGATCAAAGA ACCGAAGCTAAGACCACCCTGGAACCTGAGCCCGCTTTAAGCAGCACAGTCAGTCTAAAACCTTGTGCCGTTGCGAACTC GTCTTGTCGAGAAGAAACTCCGAGCGATGCTATTAATGAACCTGCATCTGCGACTGCACGACCACTTAGCTTGCCGATTC TTAGCGAGCCGCCTACCAACCGCGCGCACCGCATCCTATACTCATCCCAACAACTAAGGCGATACGAACCGAAGGCGTCA CCGATTAACAAGATGCCGCCGGCCAACGAACTTAGCCCGCAAGCCGCGGCTGTTGCGAGAACGGACGAGAAACATCCAAC CTCCAGAAGCCTCTTCTCCAGGTTCACCCACGGGCTGAGATTTTCGTTGCGTCGCAAAAAGAAGCAGCAGCAACAAGAGC CTTCGCAGGTCAAAGAGTCGCCAAAGGAGTTAAGGCAGAGCCCCGAGGAGAGCCAACCGGTCGACTTTGTGCACATCCCT TTAAAGCCGCCAAGAAGTTCGCAATGTGGCCGACTGGACGATACTATCGTGTCAGAGACAAGCAGTGGCCACGTGCACAG CTGGCAGCAACAGGAACAGCTGGGCAAGACCTCAACTCTGCAAAAGGTGACTGGCAAGCCGCCACTGCCCAAGATGCCAC CACGCAGTGGCATCCTAACGCATGCGCCTCAGGAAACGACCGGTGAAACTGTCAATTGCCATCGCACCGAGGCGTTGCCG AGCGTCGAACGAGAACACTGCAATCAGTTAACCGGACAGTTGGTGGCTAAGGTTCAAGCACAGAGAATGCGGGACACCGG GCTCAGCTCGCAGATAGTAGTCGCCGAAGAGACACATACGGTTAATCGACTGGCCGGACACGGCGACGCGACTAGGCCCA CTGTGGTTACCGCCGTTCCGGTTTCCGTTGCTCCTGTCGCAGAGGTCGACAAGATGGGTTTGATCGAGACCAATCTGGAC ACTCATGAAACGGTTATATCGGGAAAGACACGGTCTCTTATGGATATAACTTGCAACCCACTTAGCATGCCACACAAGCG ATACATCGTAAAGCGTCTAAATGTAAACAGCGTTGCGTATGACGTAGATGAATTGGACGCAGAAGTTAAACCCTCGCCTG GTGGAATGGCATCCGTACGCAGGCCTCACAAGAGTATGGAATTTCTGCTGGACAAGGAGAACCAGAAAAATATTCTGCCC CCTGAAAACGAACTGCAGAAATCACATGACCACAATCCAGGCGCACTAAGCGAGCATCAACTGCGTGTCCAAGCTTCGCT TCAGCGGCTAAATATTCCCGACTGGTTCCGTCATTACAACAAAGGCCCCGAAAAGGCAGCAGATGGCGAAAACGCATGCA CAAGTGCGACTGGCGGTGGTTACCGACCTGGCAACTTCACCCGCAAACGCGCACAGGAATCGGGTCGCTGGCAAGGGCTC AACTCAAAGACGACTTCCCTCAGCTCGCTGGGATCTCAACGCTCCGACCGAAGTCCTTTGTTGATGAGTCCCTCAGCGCA CAGTCATCACGGCGGCCAAAGCTCCTATTCATGTGGGTCGACGACAATTCACGGTCCGGCACCTGTACAGATAGGCATGG GTGCAACTCGCTGGTCAACCTCTCATCTGAATTCCACTCAGATGTCTCCCGGTGCCTCGCAGCGCGGAAGCTTTACGCGC GGCGCCCCCATTAATAGCAGCTTTATATCTGTGGCCAGTGGTAGTGGCTTGTTGCGCAATTCCTACCGACAGCCGTATTT GGGTTGGCGCAGCACAGAGAAGCTCTCCCAACGAACTCCCCACGAACGTCTGGCCAATTCGCTGCTAACCCAACGGACAG CTCCGCCCTCCACACGGGCGGGCAATGAAACCCGCACCTTACATTCTGTATCGACAGAGATTCAAAGCTCCATTAAGGAA GTCACATCCGCCATCGTTCACTTCGTGAACGACCAGCAGCAGTCCCAACATCCGCGGAGCCGTTCTACCAGTCCAAATTC AAGGTGCTGGCTGGAAAGCAGCTTTGTTGGTACACGAACCCTGGACTCGCCGCAAACACCCGTCATTGACAACAGTCCTC CCGAATTGGCACGAAAGCAGCAGCAGCTCCACCCGCACCAAGTGCATCTGGATGTCGGTCTGTCGCCTGTAGTCTCCGCT GGTCAGCTGCCACATCGCATGAACGGAATAAATCGTATCGGCAACGGTGGCGGTGAGTCCGCCATCCAAGTTTGATAGTT TGCCAGAGTAGAAATACTGGTAACCGGCAGTTTATGCTTTTTGTTTGGTGCACGGCATTTTTGTAGCCTATCTGCAAACA GTAAATTAATTGTGTGAACACGTAGGCATCCACACCTTTATTCCGTATAAAAATCATCAGCCTATATGAACGCAAAAAAA ATATTTTTATTTATCTTCTATAGTGGATCTTAATTGTTTTACATTTAATTATTAATTTATTAAATTCAAAGGAGCAGAAA CTTTTGCTCTTCTAATTATTTATCGAATACTCATGATTTGCCTTTGCCTCGGGTATAGAAAATTGTAGATTTGGGCAGAA AACCATATCCGTTACTTCTGCAGTAAAATGTCAAATAGATCCGCGGAAAAAAATGTAATAGGTAGAAATATGTCTGTCAC GTCTGTCCGTATTTCCATTTGTATAAACTTAGCGATAAGATTAGGCATGCAAATCGCTTTCATAATATTGGCATGAATAA TTTTTGATATTTAAATTGGCATTTGTTTTTCTTGCTTAATTCTTAATATTTGTCACGAAAAACACTTTCCCACCATCATT TAACGCCCACAAACTTTACAACTATGGGCCGATAAATTGTTCGAATGGCACTATTACTTTAAAAGTTTTAATGACGTATC ATGACATAATTGCGTCAAATACTTATGAGCAGATAGCTGAGTAACGGGTATACAATAGTCGAGGAACTCGACTATAGCCT AACTCTTTTACATTTCAATTAAACAACGAAATATGTACTAAGCCTTTCTTCTAATTTAATGATCAAACGAGACTAGAAAC CCATCTTTACTTTAAATATGTGCCCAATTGATTAATAAACCCAAAATGAGACCACTAAGAATAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.