FB2026_03 , released September 17, 2026
Clone: Dmel\GH09147
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General Information
Symbol
Dmel\GH09147
Species
D. melanogaster
Name
GH09147
FlyBase ID
FBcl0113835
Feature type
cDNA_clone
Computed gene(s)
Dmel\Pgant3
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      2,880
      NCBI
      AF145655
      Full Length sequence dataSequence Downloader
      AAATGCTGGAGAAAGCAGTAGAATGATAAGTTTAGGCCGTATTTGCACAATTACTGAGTAACTAGTGACAGCCGAACAAG
CGCCATGTATTCATAAGCACCTGCCGCGCACCTGCCAATTCGAATTTAAAGCGGCCCCGGAGCAGAAGCGACGCATTTCT
TCGCCGAGCGTCCGCAGAGTCGTCGGATCGGATCGGATCGGTTTGGATTGGATTGGTGACTAAAACAGTTGGACAACAAC
AGCGGCTTGATTAGTTGGCAGTAAACAGAGCTACCGAACGCACCGGTCATTCACTCCGCACCGTTTTGACCAGAAGCAGT
TCCAGTATTGGTAGCCAATAAGCCACAGCAATGGGTCTCCGGTTCCAGCAGCTGAAGAAGCTCTGGCTGCTCTACCTTTT
CCTGCTCTTCTTCGCGTTCTTCATGTTCGCCATCAGCATCAACCTGTACGTGGCCAGCATCCAGGGCGGGGACGCGGAGA
TGCGTCATCCGAAACCGCCGCCCAAGCGCCGCTCCCTCTGGCCGCACAAAAACATCGTGGCCCACTACATCGGCAAGGGA
GACATCTTTGGCAACATGACAGCAGATGATTACAACATCAACCTGTTCCAGCCCATCAACGGAGAAGGTGCCGATGGGCG
ACCAGTTGTGGTGCCGCCTCGCGATCGCTTTCGCATGCAGCGCTTTTTCCGGCTAAACAGCTTCAACCTGCTGGCCAGCG
ACCGCATCCCACTGAACCGCACCCTCAAGGACTACCGCACACCTGAATGCCGCGACAAGAAGTACGCCAGCGGTCTACCC
AGCACGTCGGTTATAATTGTTTTCCACAACGAGGCCTGGTCCGTGCTCCTGCGAACCATCACCAGCGTCATCAACCGGTC
GCCGCGCCACCTGCTTAAGGAAATTATCCTGGTGGACGATGCCAGCGATCGCTCATATTTAAAACGTCAACTGGAGAGCT
ATGTGAAAGTGCTGGCGGTACCCACGAGGATCTTTCGCATGAAGAAGCGAAGTGGCTTAGTGCCTGCCCGCCTTTTGGGA
GCCGAAAATGCTCGCGGCGATGTGCTGACCTTCCTCGACGCCCACTGCGAGTGCTCGCGGGGATGGCTGGAACCACTTCT
TTCCCGCATTAAGGAATCACGCAAGGTGGTAATCTGCCCGGTAATCGACATAATATCCGATGACAACTTTAGCTACACAA
AAACCTTCGAGAACCACTGGGGCGCTTTCAACTGGCAGCTGAGCTTCCGTTGGTTCTCCTCGGATCGTAAGCGGCAGACA
GCGGGCAACAGCTCCAAGGATAGCACTGACCCGATTGCCACTCCCGGAATGGCCGGCGGATTGTTTGCTATCGATCGCAA
GTACTTTTACGAAATGGGATCCTACGACAGCAACATGCGCGTTTGGGGCGGCGAGAATGTGGAAATGTCCTTCCGCATTT
GGCAGTGCGGCGGTCGTGTGGAGATCAGTCCCTGCTCGCACGTGGGTCACGTGTTCCGCAGCAGCACGCCCTACACCTTC
CCCGGCGGCATGAGCGAGGTTCTCACGGATAATTTGGCCAGGGCCGCCACCGTCTGGATGGACGATTGGCAGTACTTTAT
AATGCTGTACACCTCTGGACTGACGCTGGGAGCCAAGGACAAGGTGAATGTTACGGAGCGTGTGGCGCTCAGGGAGAGGC
TCCAGTGCAAACCGTTCTCCTGGTATCTGGAGAACATATGGCCAGAGCACTTCTTTCCAGCTCCCGACCGCTTTTTCGGC
AAGATCATATGGCTGGACGGGGAGACAGAATGTGCCCAGGCCTACAGCAAGCACATGAAGAACCTACCAGGTCGTGCTTT
GTCACGAGAGTGGAAGCGAGCGTTCGAGGAAATCGACAGCAAGGCGGAGGAGCTGATGGCGCTGATCGATCTGGAGCGGG
ACAAGTGCCTTCGCCCCCTCAAAGAGGATGTGCCGCGCTCATCATTGTCTGCAGTAACCGTAGGCGATTGCACATCTCAC
GCCCAGTCGATGGATATGTTTGTAATAACTCCGAAGGGCCAGATCATGACTAACGACAACGTCTGCCTGACCTATCGACA
GCAAAAGCTGGGTGTGATCAAGATGCTAAAGAACCGCAACGCCACCACTTCGAATGTAATGCTGGCGCAGTGCGCTTCCG
ACTCGAGTCAGTTGTGGACCTACGACATGGATACTCAGCAAATATCTCATAGGGACACAAAGCTCTGCCTTACTCTGAAA
GCGGCGACTAACTCGCGTCTCCAAAAGGTCGAGAAGGTTGTGCTCAGTATGGAATGCGACTTCAAAGACATCACACAGAA
GTGGGGCTTCATACCACTGCCATGGCGCATGTAGAGAGTAGGATGTCATGCCGGTGCCAGGACATTTAATCCAATAACCC
TGCCAGCAACTTTTTAACGCAATGTGATATTTATTGAAATTGTTTTAACGAAATTCACTCGTTAATTTGTTTATGTGAAC
AGTAACCAACCCACAAGCACACGAACTTCAGACACCTGCAGTCCTGTTGTCTAACAAAGCCTGATTTCACTGTCGCAATT
TAATATTTAACTGCGCGTAAACATGAGCAGCAGCAACATATGTGTGACAGTGGAACCAGTCTTTACCAAACAATCGTACA
CCGATACAGACCCACTTGAATTGTTTTTAAAACACACTAACCGAATGCGTGGGAGAGCTTTTCTCCCCCCACGCATTGCG
ACTCTATCAATCTAGATAGCATAGTGGTATGCTATATCTACGTCATATTTATTAAGAAGAGTTAGAAAATTTTTAGCTGT
ACTTACTAACCTCGACCTTAATTTTTATATCACTTGAATAAAGCAATCGAATGTAGAGAAAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)