FB2026_02 , released June 18, 2026
Clone: Dmel\GH10668
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\GH10668
Species
D. melanogaster
Name
GH10668
FlyBase ID
FBcl0114101
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG10165
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      572
      NCBI
      AI114018
      5' sequence dataSequence Downloader
      TAGACACCCAGACAACGATTACGACTCTCTACGCCCGCTACTCACTGTCGCAGCGTGGGAATGGCCCGCTCCTCGTAGTC
TTCGTAGGAGTTTCGTGGAACGCTCTTGTTGCGCCTGCGCAGCGATCCCTGTCGGCTGGACATTGGTACTTATTAGGCTT
GTAGCCAATTCCAATTAACTTAGCACATTAACTTACGTATAAGATACTGGTGTGCCAGGAGCTGGATGCGATAGATCTTG
GCCGGCTGCTGGAATCGGAGGATGATGTCATGTGAGGTGAGGCTGCCGTTCGGAGCACTTCGCCATCCGTGAACTGTGGG
GCCGTGATTGTTTAACTCGCTAGCCGGAAAGTTAACATCCTCGTCTGAAAGGTTCCACGCTCTTCTCTTCTATATTTAAG
CGATGTCGATGTTAACATGCTAGTTTAGTCGTCGCTCAGCCGTTCGTGTTTTGTTACTGAATCAGATAGTATTATTGAAT
CTTTTGAAATGGCGGAGGGTTACACGAAAGCCACTGCCTGGCTGCAAATCCACAGCCTGCTGAATAGCATCAACCACATT
CTGATCTTTCTG
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)