FB2026_02 , released June 18, 2026
Clone: Dmel\GH13542
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General Information
Symbol
Dmel\GH13542
Species
D. melanogaster
Name
GH13542
FlyBase ID
FBcl0117533
Feature type
cDNA_clone
Computed gene(s)
Dmel\vap
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,446
      NCBI
      BT044449
      Full Length sequence dataSequence Downloader
      ATTAAATGGTTGTGCTAATGATTCATTTACAAAGGAATCACAAACCGAACACAGCCCAGAAATTGTGAATCAAATGGGCA
GTAATTACCCCGCTTGAGAGGAGTATGATAAAGACGGCGACGGTGATGACGATGTTGATGATGATGAGATGGTGTTGATG
ATGATGATGATATGATGACTCTTCGGGTCGTTTTAGCACGGAAAACCCCACACAGTCACAAAAGTTAACGTATCTCCTCA
GTTAAACGCATTCTTATCATTGCTATATGTGGCTTTTATTAAATCGAATTCCACTCGAAGTTAAATATAGACTAAGACTC
GCATTTGCTGAACAGAGATCATAAGCCTAACAATGGCGGACCTAATACATTTGGGAAACATTGATGTTGTCATCCAGAAA
GATAAGCCCGGATCGCCAACGGGCTGTTCGGATGATGGGAATCTTGCGGGGCTGGGTGGCATGACCGGATTGGGTGGCAT
AATGGCACCTGGCGGCATCGACTTGGGTGACTTGGCTCAGGAATTGGATCACGATGAATTCGATGGACCACATATGCTGA
ATGGTGAACGTCCAGCGATTATTGCTCCTCCGGAAAGCGAATGGTACCATGGACGACTCGATCGCTACTCTGCAGAGTCT
CGCCTACGCGGAAGCAGTAAACTGGGCAGCTATTTAGTTCGCGAAAGTGACCGCAAACCTGGCTCCTATGTTCTGAGTTA
TTATGGCCGCACCGGCATAAATCATTTTCGGATTACAGCTGTGTGCGGAGACTTCTATATCGGAGGACGACAATTCATTT
CCTTAAGCGACTTGGTTGGTTACTATACCTCATGTAGTGATCTACTGAAACGCGAACGCTTGGCCATACCAGTTGCTCCG
CCGGAGCCAGTTAATGATAAGAAACGCGTCGTAGCCATCCTGCCTTACACAAAAATGCCAGAGACCGATGAACTGAGTTT
CCAGAAAGGTGATATCTTTTTTGTACACAATGACATGGGCGATGGCTGGTTGTGGGTGACTGCCCATCGTACGGGCGAGC
AGGGAATGATATTCAGAGAACTTGTTGACGATCTGGATGTATCTATCGATCCAAATACGGTGTTTCCCTGGTTCCATCCC
AACTGCACCAAAAACGAAGCCGTCGATATGTTGGTTAAAGCTGGACCAGGAAGTTTCCTCGTTCGCCCAAGTGACAACTC
ACCGGGGGATTACTCTCTATTCTTTCATATAAACAATCAAATCCAACGCTTTCGTATTGAAAAGAAAGGCGTGCGCTATT
TAATGGGCGGTCGTACATTCGAATGCTTGGATGCGGTTATAAATCGATATCGCAAAGAGCAAATTGTGGAGGGCCACTCA
CTGAACCATCCAGTTGTCAATGGCATTCAGCCCGAGTTCAATCAGCAATATGTTGTGGAAAAGGCCGCTGAAAAGATCTA
CGCAACATTGCGCGAGTGTCGTGACCAAATCGGTCTCAAAAAGATCAAGGGAATCAAACATCACGGCCATTTGAACAAGA
AGTCGGACAAAACGACGAAATGGAAGCAACTCTATTTCGCTTTAATCAACGATGGTTCCGAGACGCAACTATGCTTCTAC
GACAATCCGAAGAAGACCAAACCGAAGGGTCTGATCGATTTGTCCTGTGCTTACCTCTATCAATGTCACGATTCGCTGTG
GGAGCGTCCCTATTGCTTCCAAATTGTCGAAAGGGCCCTGCCATGCTTGGCCACAGTGACGTATCTGTGTGCTCCTAGTC
AGGAAAGCTACGTGGAGTGGATTAATTCGCTCAAGGCACAATGCGATTCCCAGCTGAGTCGTGCCCAGAAGAAGGTATCA
CGCCTTCGTGAGCTTCGTTGTCTTAATCTGCATGTGCTGGAAGCTCATCGATTACCATTTAAACTAGTGCCACATCCATA
CTGCAGTATTTCCCTCAATCAAGTCAAAGTGGGCAAAACTCGTGTCAAAATTGCACCCGAACCCGTTTGGGAAGAGGAGT
TTGTGCTAGACGATGTTCCGCCAGATGTTGTCTCCCTAACCATAACATTAATATCGCGCGGCAAACGCGGTAAGGACTCC
GAAGTGGCTGAGCTAACCATTGACTTGTCTAGTCTAAAGAATGGCCAGGAAACCGAGGGCTGGTACCAACTCACTGGCAT
GACGCCGATGGGTGAATGGGGCTCGCTACGATTGCGTATGCGCTACCTCGACGATCTGATAATGCCGTGTGAAGAGTACA
GTCCTCTGCAGCAATTGCTCCTCGAATCGGAGCTGTATGCTGTGAAGGCGCTGGCTGAATTGTGCCATAATGATCGGGTT
CCTTTGGCCACGGCTCTGTTGCGAGTCTTTAGGCAGGAGAAACGGGAAACGGAACTAATTCGAATGCTGTGCCAAGCGGA
GGTGACGCGCGAAAACGAAACCACAACGCTTTTCCGTGGCGCATCCTTGGCCACCACCCTAATGGATCTATATATGCGCA
CCGAATGCAGCGGCTTCCTGCAATCGGCCGTCAGCGAAACCGTGCAGCGCATTTTGGAGAGCAAGCAGTCGGCTGAATTG
AATCCCACGAAAATGGACGTCAACGATGATGCCTGTACGAATGCCGAGTTTCTATTGCAAATCCTCGATCTGGTCACCCA
GTCGATATTCACTTCACCGGACGCCTGTCCACGCAACGTGCGTTTCATTTGCAGCTGTCTTCAGAAGGCTGTGATGGCCA
AATGGCCGACAGAGCGGCTCGTTCGCACACGCGTCGTCTCTGGCTTTATATTCTTGCGCCTTCTGTGCCCAGCGCTGCTC
AATCCACGCCAATTTGGATTGGTCAGCGAGACGCCACCGATGGCAGCAACACGATCTTTGGTCATGGTCGCCAAGTGTCT
GCAGAATCTGGCCAACTTGATTGAATTCGGCGGCAAGGAACAATACATGGAGGTTGTCAATCCGTTTATCTTGAAGAACA
AGGAGCGTATGATTGTTTTCTTGGATCAACTGTCCTCGGTGAACGATCCGAACCAACCGCTTGGAATGTTTGTCGAACAA
AGTACAAATCTGAATTCACAAGACACTGGACGCGAGCTGGCTACCTTGCATCACATCTGCGTATCTCATTCGCAAGAACT
GCACGAGCTATCCAACATACTTTCAATTAAGAAGCTGGTCACAGTGACGGACATGCTGACCAAACACAAGCTGAAGTATC
GCGAGATGATCAGCTAAAATCAATTGCGAGGAGAGCGGATAATAAAAAAAAGCGATGTCTTCTTATCTACAAGTCCCTTC
ATACCTTTTTATAAAAATATTTATATGTGTATACTATTAGAATACATAAAATAAGAACTCAAAATAACTGGTTGAATTGT
ATTGGAATAGTAAACAGCATCTCATTTATAAAATTATTTATATGGAAATGAAATAATATTTAACAGAAAAAAAAAAAAAA
AAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)