FB2026_03 , released September 17, 2026
Clone: Dmel\GH01474
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General Information
Symbol
Dmel\GH01474
Species
D. melanogaster
Name
GH01474
FlyBase ID
FBcl0117659
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG1486
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,392
      NCBI
      AY069032
      Full Length sequence dataSequence Downloader
      ACGAAACGAAAACAATTATTTTGCATCGCAGGCGATAAGTTAAAAGTGATTTAGACCGCACTGAAACCATGTCAGCGGCT
GGTAGATCGGTGGACGATCCCGGCCAGAGCCCACTCGTAGACCAAGAGCCGGATCCGGGCCAATCAACGCCAGCGGTGGC
CGAAATCGCCGCTTCCAGTATTCGATCGGGCCTGGCGGAGCTGGAGCTGCGATCTTCGCAGGTGCTGCAGCGCTTAGAGA
ACGTTAAGGTGTCGACCACGCCGGAGAGCCCAAATCCCGTCGAGAACGAGGAAGAGGCTGGGGAGCGGGTGACCAGCGAG
GACCTCCTGCCAGCGAAGCATCGCGTGCCCTCCGACATTCTTAAGTCGCTGGAGCAATTGGTTTCTTACACGGACAGCGA
CGATGATCCGGAGTTCCCGCTGCCCGCCCTGGATGACGTCTCCCACTTGGCTCTCATATCGCACAGCATTGTGGCCTATT
TGTCGCATCTGGACCGTCAGCAACTGCTCCGGGTGACGAACAGCATTTCCGGGGACGCCACCCGCTGGTTGGGCACGCTG
TTTCACTTTGCCCACCCGGCGAGCAGCTTCCATGCGGACAATGCAGACGCGGTGCTGCGTACGGTGCGACTGGCCATCGT
CGCCAGGTGTCCGGGGTACTTGGAGGGTGGCATACCCGCCCTGGCGCAGCCTACGTTTTACATCTCGGAGAATACGACGC
CGATGAGGCTGCACTACGCATGCCGCCAGTTGGGCATACCGCTGGAGGCTATTAAGGTCATTCCGGAGCACAGTCAGTCC
GGGACAATGGATGTGACGCTGCTGCAAAAGCAAATTCAGCAGGATGTGGGCAATAACAGGACTCCGCTGCTGGTGGTCGC
CGACATTGGAGCCTCGTTGTGTGGCTATGTGGACAACCTGCTCCGGCTGAGGGACGTATGCAAGGCGCACAACATGTGGC
TGCACGCCAGTGGCCACGGACTGGCCGCCTTGGTGTGCGCCCAAAATCAGGGCCATGTGGAGGAGGTACTCCACTCGATG
GCCCTGAATTTGGGCAGCTGGCTGGGTGTGCCCAGTCTGCCCATCGTCCTGCTGCACCGTCCGCTGCAGAACAGCGCTCT
GAGTGCCTTCGAATCCGATCCCATACTGTCGCGTCGTCTTAATGCCCTGTCCTTGTGGACCAGTTTGCAGGCGCTGGGCC
GGAAGGCGATTGCGGAGCGTCTGCATGTGGCCTTCCAGACGTGCAGTATTCTCTTCGAGATTGCCTCCAAGTGTGAGGGC
ATCCGGGTGTTGAGCCATACTCCAGGAGCGCAGACCGGAGCCTCTTTGTCGGACGTCATTCAGAGCCCCTTTGATGTGCA
GGCGCTTTTTGATGCCGCCGCCCCCGTGGTCGCCTATCAGTTCGACGGCAGCACCACCATTCCACTGGGCGGGAGTGGCT
CTTCCGCCGCCGCTGCCGCTGCTGAGCGGGAAACGGCCGAAGGCCTCAAGCCGCTAGAGAAGATTAACAATGCTTCCTAC
TTCGACCGGCTCAACTCCTGGCTGGGTCAAATCTTGCAACGCGACTGTCCCAATTTTGACTTTGAGGTAATTGAACACCC
GACGCACGGCAGCTGCATTCGCTACTGTCCGCTGGAATTGGGGTTGGGTGAGCAGCCGCCAAGCTCCGAGAACCTGGAGA
GCTTCGCTCAGAGCTTAGAGGCACATGTGGACATCCTGCGCGCGACCATCAAGCACAAGGCACGGTTCATTCATCTGGTG
GAGTGCAGCGACGTGCTGCGCCTCGTTCCACTGCCCGAATGGGCGGGCATGGGCGGTGTTCGCTTCGTGCCCGAGGGCTG
GGAGTCCCTGCTGACGGACCAGGCCAAAACAGAGCTAAACAAGCTGAACATCGACCTGGTGGAGGCCCTGAAATCCACGG
ACAATGCCTTTTCATTAGGCGAAGGCACCGATGGTCTGATTTGTGTGCGATTCGGAATGGTGACCCATGAAACGGAGGTC
GAAGAGTTGTTAGACTTGGTGGTCACGGTGGGCAAGAGCGTCCAGGAAAACTCGCGGGTATTGGACACCATGTCGGAGAT
TGTTAAAAAGGGCATTGAGGCGGTTACCGCTGATCTGCAGCGCGAATCGGAGGAGAAGCTCTGGCAGGAGGGCATCCTGC
GCCACGTGCCTGTGGTGGGACGCGTCTTCAACTGGTGGTCCCCACCGGCCAAGGAGTCGGGCATCAAGGGGCGGAGCCTC
AATCTTACCCAAGGTGTTGTCGAAAGCACCGAGAACATTTACAAGTACCACATGCAGATGACCGGAGCCACTGCTCATCA
GTTGCCAGCGAATCGTTCGCCTCCCACACCCATGGTGCAGACGCCCGTGGTCGCGCCTGCCTTGCCGCCCGTCTTTCCCA
CGGTGGAGCCCGTACCCGGAAATGGAAACGGATCCGGAGCGAGCGAGGGCACGCCAGTGCAGTCGGGAGAAGCTTCGGGA
TCGATGCCAGCAGCCTCGGGAGCGGCTCCGTCTGCGGCGCCGACCCAAAACCATGTGGATCATGCCCGCACCGTCAGTCA
GAGCAGTGCCGCCTCGTCCACCGTTCCGGAGTTAGTGGCCGCCAGCAGTGCAATTAACAATAACTAACAATAACACAAGT
ACGAGTATATTTCAAAATTAACTATAACTGAACGGCAGAGGAAAACACTTAGATCAAGGCAGAGCCAGCAATATGGATTC
CACAGTGGGAGAGGCCTTAAAACGCAGCAATATCAAACAAGGCTTGAACTGAACAGAAACGCGTAGACAAAAACTGGACA
GATTACCATATATATGGACGGCTTCATTTCCACTTTCCCTACTTAAACGAAAAGAATTCCAAAGAGGATGATTGTTCGCC
CGAAAAGATGAAAAGATGATGATGATATGATCCAATTGAATTGCTTTTATGTATTTATATGTATATGGCTATTTCTATGA
TCTTTTATATATCTCCATTATATATTATATTATTTTACCTTATACTTTACTCCCACCATTATTGTTGATTATGATCAGAG
GGAAATGTGCGTAACTTCGTCCTCTTTACGCTTTTATTTTTTTGTTTTTATAATTATTCAGATGCAAGTACCTTTGAGAG
ACATAATATATATACATTTATATATATATAGGCAATTCTTCAATGTTCAATTGTGCGAAGCAATAAATTGCGCTATTCTT
ATTCGTTAAGCTAAGTGACAGTAAACTAAAGAAATTAAAGAAAACGCGGAGAAAGAAAATAATTAAATGACAAAAATATA
TTTATTATACAAAAATTGCTGCTATAAGAAAATATCTGCACAAAATATGATAAAAGGAAACTATACAAAATAAAATGTGA
AATCATTTAGAAAGAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)