FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\GH20028
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General Information
Symbol
Dmel\GH20028
Species
D. melanogaster
Name
GH20028
FlyBase ID
FBcl0117673
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG6204
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI07760
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,797
    NCBI
    AY058421
    Full Length sequence dataSequence Downloader
    ATTTATTTATTTCCAAGAAATCAAAACGAAGCCCCATGTCAAGTTCAGATGACGATTGGTTTGACCAGGATGAGAACAAA
CTGTTGCAGGGCCTGGAGAAGTCCTTGAAGTCCCTGGAGCTGCAGAAGAATGAGGAGTACATCGAATGCCCCCCATCTGA
GCGTAAATGCCCCCCATCTGAGGTGGGGGAGTACGTGATGCAACATACACGGTTCTCCCTGACCGAGTTAACAAATGCCT
TAAAAATGCCAGCCATCGACATGTTCTTATACTTTTTGTCCGATAAGCGAGATCTCTTCGAGAATCAAGTGTTGGCCACT
GACAATGTGAAACGAGTTGGCCTGTTCGTGGATGTCCTGTGGTCGCTCTGTGAACTCGAATTGGGCGGATTCGATGAAGT
CTTTCTGTCCGCATTCAGCCGGCAGACGGCGCTTCTGGACAAGATCAAGAATCTTTTGCAGGCCAAAGCCGCTGTGGCAA
AATGCGATGCGGAGTCGGCACTGATATTAAGCCATAGTAAGTGGATGCTTCTACGAGCCCATAAGCATGGCCTCCTTAGT
CACCAGGGCTACGAATTGGTGGAACTTTATAAGAAATTGGCACCTTCCTTTAAAAGCGACATGATTGATGGTCTTGAAGC
ATTCACCGGTAACTTTTCACATAACGTCAAGGGCCTAATTTATCCAACGCTGGAGACGTTACTGGGCAAAGATGCAACTA
AGGCTCCCAATGAAGAAGAGGATGAGGGCTTGGTGTCCGACAAAGTAGTCAAATATGTGAATGCACTGCGAAATTTACTA
AGGGAAGATTTTTTAGCACCACTAGTTGAGTTTGTGCAACAGCTGCGCAGCGGAACGGATGTCGATGAGTTGAAGCAACA
GGGCCTTCTGTGGTCCGATGTGCATCTGACTTTAAATCCACAGTTTGCCAACGCTCAGCGTCATAGCCTTGTTTTTTTGA
AGGTTCAATTTACTAAAGAATCCAAGAATGCCTATAAGACTTGGCTGAATTCTATCAAATCTGGGACTCTGCTCTGTCTT
ACCACGAGTCTGGCCTTTGATGATTTAATTCTGGCTTCCGTTGGCTACACTGAGCCAGAAAAACTAAAGGAGGATTGCCT
AAGTGTGCAGATTGTCAAGCAGTATAATATTGGAAACGCCTACAACCGACCACTGATCATGTTCCAGGCGCCCGTGTTTT
TCGAGCCTTATCTCAGAGTTCACAATTACCTGAGCACCTGCAGCACAGAGAAGTTTCCCATGGGTCGCTATATTGTAGAC
GGCCAGATGGAGATACCGCCGCCAGCTTACATGAAACCAGGAGTTAAGCTTAGTTTCAATATGAAGCCCTTCACGCTGGA
CAAACTACCGGAAGATCTGCACTTAAATGAGAGTCAGAAAACTGCATTCAAGGAAGCTTTATGCAGGGAGTTTAGCATCA
TTCAAGGACCTCCAGGCACCGGAAAAACACACCTTTCAGTGCAGTTGGTTAACAGTTTGATACAGAATGCTAAAGCCCTG
GGCACGGGACCCATCATTGTGCTGACCTATACCAACAATTCGCTGGACAAATTCCTGGTAAAAATTTCGCGGTACACCCA
AGAAATTCTTCGCTTTGGTAATCAGTCGCGAGATCCGCAAATATCAAAGTTTAATTTGAGTACCACGATCAAGCCAGAAT
TGGTTCCACCGCGCCTGAAGCGAATTTGGTGGCTAGTCAATTGTGAGTACAAGGAAAAATTCCGGAACCTACAAGGCCTG
TACGCAAACTTTGATGGCAGCGAGGAAAGCTACCAGGACACTCTGGCGGCTCAGGAGAAGCTAAATCAGGTTGCCGAGCG
TATCGAAACACTGCGCATGGTTTTCCAGTTCTTTCTAGCAAGGGAGAAGGACCTGTTGGCCATGACCACCACGTGCGCGG
CTCGCCATAACTTTCTGTTCAGGTTGCTACAATCAAAGTGCGTCCTCTTTGAGGAGGCAGCCGAGATCCAGGAGGCACAC
ATCGTGGCATGCCTAACGCCGCACACAGAGCACGTGATCCTCGTCGGCGATCACAAGCAGCTGCAACCTTTCAGTGGCAG
CAGGAAGGTACCACAGATCTCGCTCTTTGAACGACTCATTGTGGCGGGGCTGCCATTTTCACGGCTCAATCTGCAGTACC
GCATGCGATCCTGTATTTCTGAACTCCTTGTGCCCAGCATTTATGACGAGCTGCTCTGTTCGGAGTCAGTGAAGGAATAC
GAGGACATCCGCCTGATGTCTAAGAACTTGTACTTCGTTCAGCACAACCAACCTGAGCACTGCATGTCTGATATGTCCAT
TGGGAATCTTTATGAAGCTGGAGTGTTGGCTAAATTAACTGAGTTCCTAATCCAGAAGGCCCAATATAAGCACAGCGATA
TTGTGATATTATCCCCTTACAATGGCCAAATAGAGTGTATCAAGAACGCGCTTCCCCAAAACTATCGCTCGACTGTGCAA
GTGGCCAGTGTGGATAGTTTCCAAGGCCTTGAGGCCAATATAGTGCTGCTCTCGCTGGTGCGCAGCTATATATCCGGCCG
AATTGGCTTCCTTCGCCAAGCAAATCGAGTGTGCGTGGCTCTTTCTCGAGCCCGCTGGGCCCTGTACATCGTCGGTAATG
TGACGATTTTGAAGGATACCTTCCCAAAGATTTGGAATCCAATTGTCAAGCGCTTGAAAGAGAATAATGCCATCGGAGAG
GCATTTCCGACCATAACCAGTACCTGATTACAACTAATTAAAAAATGTATATATGTAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)