CAGCAACTGGTTTATGTGAGCCACAACAAAATGATCGACAAAACCTTGTTCTACCACAAATATTAACAATTACCAATATA AAGGTATTTAAACAACTACCTTGATCACAATTTTAGTTAAAATTATGCCAACGAACTGGAACCAAAATCCCAGGTGAACC GCAAATGTAACCAGTTCACTCGGCTGGGTCCAAAGACCAGTTCAATTAAACTAGTTCACATTTGAACAGTGCAACTCTAG TTTTTTTTTTTTAATACATATGTGTTTAAAAAACATATGCAGAAACGGGCTAATAAAACGGATATAACCGATTGCACAGG ATCAAGGATAGATCAAAATAACCGACGACGTTAACGGCGACTGCGACTGCGAGAGAGCGACCATGGTGTGCAGCAGCGCG TGTGCGTGTGTGTGTGTTTTGTACCGTGTTGTCTGATTGTGTGCCGTTGTTGTTGTGATTGTTGTTCTCGCTGTTGCTAT TGTTTTTGCTCTTGTGCGTGTGGTCAACGGACGGACAGACGGACGGACAGTGGGACACAAGGAGAGTGGAGAATCCGAAT CCGGTGGGCAGGAGAGCAGGATTAGCCGCAACATCCTTACCCCGAAACAAAAGCAAGTTAAAAAGCGAAGGAAGCAAAGA GAAAACAACAAAAAAGGAAAAGAAGCAGAAAACAAAAACAAAGAAGAGATTCATGGTTTCCATGTCGCCAACTGTGCGCC TCTGATGCCCTCGCAGGTCTCCCGAGGATTGGACACCACCAACATGCTGCCGATCAGCGAGGAGCAGCAGCTGCAACAGC AGCAGCAGCAGCAGCAGTTAGAGCAACTGCATCATCCGCAGATACCAGAGATTCCCATACCAGATCTAGAGCAAGTCGAG ACCCAGGTGGGCGATGGCAGCCTGTGGACCGCGCTGTATGACTACGATGCCCAGGGCGAGGATGAGCTGACACTGCGACG CGGCGAAATCGTTGTGGTCCTGTCCACGGACAGCGAGGTGTCTGGCGATGTGGGCTGGTGGACCGGCAAGATTGGAGATA AGGTCGGTGTCTTTCCCAAGGACTTTGTCACCGATGAGGACCCACTGCAACTGAATGTCTCGTCCGCCATTGGCGACATC CAGCCGCATGAGATCGAATACAATGAGCTGGACATCAAGGAAGTTATCGGCTCCGGTGGCTTCTGCAAGGTCCATCGTGG CTACTACGATGGCGAGGAGGTGGCTATTAAGATTGCCCATCAAACGGGCGAGGATGATATGCAGAGGATGCGGGACAATG TCCTGCAGGAGGCCAAACTGTTTTGGGCCCTCAAGCACGAAAATATAGCAGCCCTGCGAGGAGTATGTCTGAATACCAAA CTCTGTCTGGTGATGGAGTATGCCAGGGGAGGAAGTCTCAATCGCATACTGGCTGGCAAGATACCACCCGATGTACTAGT CAACTGGGCCATTCAGATAGCCAGAGGCATGAACTATCTGCACAATGAGGCGCCCATGTCGATCATACATCGCGACCTCA AGTCCTCCAATGTGCTGATCTACGAGGCCATCGAGGGCAATCATCTGCAGCAGAAGACGCTCAAGATCACGGATTTCGGT TTGGCCAGGGAGATGTACAACACGCAGAGGATGAGTGCGGCGGGCACGTACGCTTGGATGCCACCGGAGGTCATCAGCGT TAGCACCTATTCCAAATTCTCCTATGTTTGGAGCTACGGTGTCTTGCTGTGGGAACTGATAACGGGCGAGACGCCCTACA AGGGCTTCGATCCGTTGTCCGTGGCCTATGGCGTGGCTGTTAACACGCTGACATTGCCCATACCAAAAACCTGCCCCGAG ACGTGGGGAGCCCTGATGAAGAGCTGCTGGCAAACGGATCCGCACAAGCGACCGGGCTTCAAGGAGATTCTCAAACAGCT GGAGAGCATCGCATGCTCCAAATTCACCCTAACGCCCCAGGAGTCGTTTCACTACATGCAGGAATGCTGGCGAAAGGAGA TCGCTGGAGTGCTACACGATTTGCGCGAAAAAGAAAAGGAACTGCGCAACAAGGAAGAGCAGCTGCTCAGGGTGCAAAAC GAGCAGCGCGAAAAGGCGAATCTCCTGAAGATCCGGGAGCAGAATCTGCGCGAGCGTGAAAGAGTGTTGATCGAACGGGA GCTTGTCATGTTGCAGCCGGTGCCATCGAAACGCAAACACAAAAAGGGCAAAAAGAACAAACCACTGCAGATATCGCTGC CCACGGGCTTCCGGCACACGATTACGGCTGTACGCGACAAGGCGGAACAGCCGGGATCACCTTCCTTCTCCGGATTGCGC ATCGTGGCACTGACCGATGGGCACAAGGGCAAGACGTGGGGCCCGTCGACGATGCACCAGCGGGAGCGATCCCTATTGCC TTCGCAACTGAGCGGCGGTCAGCCGGAATGGCCCGCCCAGACCTCAACTCACTCCTCATTCAGCAAGAGTGCTCCGAATC TGGACAAGAAGCAGCAGCAGCAGAACCAGCAACAGGTGGCCTCGCTGACACCGCCGCCGGGTTTGGGAATTTTGGGTGGA AGTGGAGGCGCTGGTGGGACGCCGGCCACACCATTGTTGTACCCAGGTATACCCATTATACTAACGCGCCCCAACAACAA CAACATTGGCAATTGTAAAGCCATCACCACCACCATCACAACTACCACCACCACAACCACCAACAACAACAACAACAACA ACAACAGCATCTCCGCCAACAACAACAATCAGCTAAATAATATTAGTACTATTAATAGCAATAACAATAACAATCAAACT AACCTGACCTCCCAACCGAACACGATAATTGTCCTCCAAAACGGCCGGAACAACTCCAACAGCAGCACCACCAGTCAATC CCCAGCCAAGATCTACCATCGCGCCAGGAGCCAGGAGTACGGACTTGATCATCCGCTTGCTTATCAGCCACCACCACTCT ATTTGGTAACGGATGACAGCTCGGAAACGGACACCGTTGCCAGTCCGACCGGTTGCTTTCACTTCCTGAAGTCCGGCAAT TCATCGGCGGCCAGTGGTGCCGTGCATCTGCATCGCTTTGGCGGCAGTCTGGGCAACAGTCCAGCTGTGGGCAGAAAGAA GCATTCGCTGGACAGCAGCAGTCATCATCCGCCTGCGAACGGTAGCAATAGCTTTGCACTGCCCAACCAGTTAACTCTGC CATCGGAGGATAACAATACGTATGACCATGCCTTCTATCGGGATGTGATCAAAAAGATGTCCATGGCCAGCAGCGAAAGG GTAAACTCCAAATCCAGTGGCGACCTGACCATGTACAACTCCAGCACACCGCTGACTGCCAGGGATTGCGATGATGCGGA GGAGGCCTTCGAGGGTGGACGCTTTCAGCGTAACTTTTCCGGCTCGCAGTTTCCACGGCACTGCTTCTTCACCAGGCAGG AGGAGGAAGGGGAAGCAGAGGATGAGGACGCGGTGGCGGCGGAGGTGGACACAGCAGATGCGGATGCCGACGATGAGTGT CAAGTGCCCGCCAGTCAGATGCGCCAGAACTCCACCACGTCGCGCAAGAGTTCAGTGACCTTCCAGAGCGTCAGTTTCGA AGAGCCCGATTTTGTGGCCACGCCGCGAACGACTGCCAGATCGGATCTATATACATCCAGTGCCTCAATTAGCTTCGCCA CCTACCGCTCCGCCTCGCCATCGCTGTCCTCATCGTCCACCACTGCGTCCGCCTCACCGTCCATTGCTTCTACGGAAGCC GTGAACGGCTATCACATGCAGGAGAACTCCATACTGAATACGCGTCGGATGCAAGATGTTCAGCCGCATCCCGATGTCAT TAAGCTGCGGGCCCAGGAACAGCGCCAGCAGACCAAGAACCAAAAGAAGCAACGGCCCAAGCACATAACCAAATCCAAGT CTGTTGAGGCGCCAGTCGAGGGACAGCACCATGAACATGATGACCACAATGATCCCCAGCACCAGCATCACTCGGCTGGA TCGAGCAAAATCCGTGCCCTTTTCAATCTCTTTACGCGCTCGCGAAAGAAGTACAGCAAGCTGGCGGAGCACAACATGGT CGGTGGACCCGAGTTCTGTGCCATTGATCCCTATCAAACCGATTTGGCGATGGGGGGCAGCAGTCGCTCTTTGAAGCGCA AAGGCAAAAAGCCGCAGACGCAATCCTGCGAGCAACTGGAGCGATGCTAAAGTCCCAGTTGTGCGCCGTGCTGCAGTGTT GCTGTTGGAGTTGGCCGCCAGATGAGAACCGCCTAATTAGCTCATTGTTTTATATTAAAACTGTAGACTAAGTCAGACGC AAAGGAAGAGCGAAGAGATGAAACAGCAGGCGAGGATTAGCCATAGCTAATGTGTGAAATTAGTAGGGCCCAGAGATACA GATACCTATCCGAATTCTTTCCGTCTAACGAAATCATAGAACTAAACAAAAAACCCCTCTGTCCGCGGTTGGCATTTCAT AATAACCGAATTGTTCCTAACGAATGCAATATGTTGTGCGAAATTCACAAATCAAGTTAATCCCTAATTAGTCCAGTGAT TTGTTTTGTTTTTTTTTTTGTGCCAGAGGAGTGGCTAGAAAATTGCAATGTGTAGCACAGAAGTGAGGACGAACTTTTGA GGATCAAACGGATAGATAGACTAACGCCGCTTGTACGTAGACAAACAGACACGGAGAGAAAAAACGTGTGCATGGGCCAA TGGCAAAATATATTGAACTAAACCATATACATAAATGTGTAACAATTGGATTAACATTAAGCTATATTTATACCGATGCG TAAGTGCGTCCTTACACATTACACATTACACACATCTTAGTTGAACTAAGTTCTAGTTTTGTAGGAACTCGAACATTTTT TTGGAGTCCTTCCTCCTTTTTTTTCACCCCCTCAATCTTTGTAAGAGATATTTTCCAAAATTTCCATCGCTTGCGGCATT TCGTTTGGCTTAAGTACACTTAACGAAAAGTTGCGGTGCACATTAATTTTAATTTGATATTCGACTTTTTTGGTATTTTG AGCACAGTTTTATTTACATACATTTATTATGCAATCGGGTTTTCCATTTTATTTATTTATGATTTATTTCTACAAAAAAA AAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
