FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\GH05095
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General Information
Symbol
Dmel\GH05095
Species
D. melanogaster
Name
GH05095
FlyBase ID
FBcl0120427
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG6424, Dmel\CG46315
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI21218
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,579
    NCBI
    AF181632
    Full Length sequence dataSequence Downloader
    TAAGGCTTGCATTTGCGGCATGCGCCATCCCACAACTGGGCTGAACGAAGACGACCTGAGTCGTCTCACTGGTTCATCAT
CGTCGACGGCTTCGTGCGGCAGCGCAGCATCCTCATCATCGTCCTCATCCTCGTCGTCCTCGAATCCCGCTGACCGACAT
GAGGTGGTCTGCAGTCCGGAAAAGCCAACGCCGACGGGCACGGACAAGAGCCAACTGAGCAGCGGCTTTAACTCCACTTA
CCTGCCGGAAATCATACATCCGGCTCCGGAATGGCAGAAGTCATCGCGCAAGCGCAAGGAACGCCTGGACAGCACCTCGG
CCTTTGGCCAGGATCGAAAGTTACAGCGCAGCAACAGCGAGGAGCTGCTCACAGAACCAGCTGCCATTGTGTCTTCCACA
GCCCAGGCAGCAGCCGAACTTCTGGAGGCCCAGTGTGAGGTTATAAGACGGGTTTCTTCGGAGGATTTCAAACGCACGGC
CAGTTACGCCAACTATCGGCAGGAGTTCGAAAGGGAGCAGGAGGAGTGCTCGGAGCTGGGTGAGAACAATGCCTCGTTGG
CCAACCTGCCGACAGGAGAAGCAGCCAAAGAGCGTCCTCGTCTTGAGACAAGCCCCGCTCGCCAGGCACCAAAGGAGGCT
AGCGATGACTCCGAGTGCGAGCACGAAAGGCGACGCAGCAGTGAGCGTTTCTGCAAATCCCGCCAGCCGCCGGTGCGCAA
GTCTGGCGTGGCCAAGAAAGTCGGCGGAGGCAGTGGCTCCAAATATCTGCCATCTCGACGGGATGAGCAAAGCGCCTACA
AGTACGACTTGTCGGCTTTGAAGTACGAACGCAGAGGTTTCATTAGCAGCAGGAAACAGCAGCAGAATCAAACACAAGCA
ATGCAGTCTGCTGCCGATCACAACGACAACAACTTGATAGACTTCCATCAGCAGCAGAAGGAGCTGCAGTCGAAGCGGAG
TGCCAGTATTTCCCCCACACCCACCACGAGCTCCTCGGCGGGCAGCGATGACAGCACGCCCACTTCCAATGCACCCGCGC
CCATCAAGGCCAAACCCCAGAGGCAGCAGCATAGCCTGGATCTCACCTCCGCGCAGGATTCCCTGCCGTGGGAGCGTGGA
GATGAACCGGCACCCATTCTGAGCAGAAGATACGCCCACTCCCGACCACATATCGGTGGCAATCTCGATGCGGCCGCCAA
ATTGGCCAAGGTCATGCCATATCCCCAGCAGCTGCCCAAACAGGCATCCACCGTGCAGCTGCACTCGGACATCACCGACA
TGGACTGCTGCCTGGAGCCCATGGATGCCGTGCGAGCGTTCAGTTCGCTGGAGAATATGCGAACCCGCGACGTTATGCAA
CAAGTGTTGCCCGCCATGATGGGTGCATTTCAAACGCCCGAGGATCGAGTGAAGGCCATCAACAGACGCGTTACTGTGCT
CAAGAAGAAACTGGTTCAGCTGGAGGAATCCCTGGAGCAGCGACTGGGCTATCGGCCATCTCAGGCGGAGCGATTGAACG
ACAAGTACATGAAGAATGCCCTGGCGGAGTTGAGCAAGCTGCGCAAGGAACGGCACGAGCTCAAGACTGATCCCATCGCA
GCTCTGGGTCTGAAGGTGGGCAGCGGCGGTGGCGCCGGCATCGGTGGCCAGGAGGTCGCCAAGAAGCTGGAGCGCATGAA
AGCCACACTCGCTGAAATCGAGCAGAATTTGAACGAGAAACGCGTGAATGGTCATCGTTCTGAGCAGCTAGAGGATCTGA
ATGCCGACCAACTGGTGCAGGAGAAGAGCGCCGTGCAGCATGGACTGCTGTACTTTGAGAGCCTCTATGGCCATCCCATT
ACCAAGGAGGAGCGCGATGCTGCCCGGCCGCTCTACGATCGCTACCGGCAGCTGAAGCGTCTGGTGTCGCGCACCGTGAT
GTTTGGAGCGGGCACAGGGATACCCGAGTTGCCCACAATCCTGGAGCACGAGGCCATGGTGTTTGAGGCCACTTCCACAC
CGCTGCAGTATTCGTCGAACAACAGCACAGAGACCACCAACTCGCCCAGCGACTCGAATGCCCCGAATACTCTGACCCAG
AATGCCTCATCCGACGAGTCGACGACCACCACCACGTTGACGGGTCCGGCAACGGGAAATTCGGCCACAAGCCAGGAGAA
CCAGGCCGCCAGCGAGAATATCGCCGCACTGAGTGTGGACCAACTCTGGGAGCAGCTAGATCGAGCACGCGATGAGAAAT
CCCTGCTAAAATCCACCATTCGGGAGTACGAGTCGCTGTTCGAGGAGCAGAATGGACGCAAGATGCTCAAGCAGGATCGT
CGCTCACTGGAGACGGAAACCTATGCCCAGTACAAGGAGAAGAAGGCCAAGGTCAGACTACTGCAGGCCCTGATCAAGAA
GCATATTGGGCATTAAGCTACGGCTACAGATATCCTTCTTCCATTCATCCACGCATGCAAAACTATTTATTTCATACCCA
ACCAAACAAATACAGACCTAGATCTATACAAATTACATGTATTGTAGTTTTTAGACTTATATTTACCATATATTCTATAA
TGATACATTTTATGAATATTGTTGATTCCTTATTGTTTTTTGAAAACACATCTATTGAGTAATTGTAATAAAAGAAAATT
AAATTTGATGAGACAAAACGAAAAAGGAGTGATTGAGAAAGCAAACGCTAGACATTTGATTCAGACCCATTCTCCTCTCC
ACATTATTCGCACGGAATTGATATCTATGTGTAACCCAAGAATCTGCAATGTATATGTTCGCACTCTTCCCATTTGTGTT
CTTGTAAATCGTCATTGATGTTAAAAAATGACTTGGCCTGATTTGTCCCACTTCTGAAACGGGGAGCACACATTATTCGA
TTAGCTATAGTTTCGAACGATCTATGCGTGGAGGAACACGTTGCAACCGCAAATCAAATAGATAAACTTTATAAATGTTG
TCTAGTAGAATTGAACATTTTATATCGATATGCAAACCACTAACTAGCAACCTATTAACCGAAATACCCGAATTATAGTT
GTGCTTTCTTCCATTAAATGTTTCAGTGTGCAAATGGCACGCAACCACGCACGAAAAATCGAAAACATCTGAATATTTGA
TAGCGTTGGAAGCGGAAGTTAAGTTTAGACGATCATAAAGTTAGGTTCGTACACTGGCCGAAAAAGTCGGGCAAATGATT
TTTGTGCAACAAGCAATTGGGTTTTTTTGAACGTGTATACTCGGCCCTAACCAACGCATACAATATATGATTATACATAC
ATATATATATTTACAATGATAAAGAATGTAAGGCGCAAGCCAAGCAAACACATATGTAACGTGTATTTGAACCACGTACT
TATATATTTACATGTTTACATATACGAACATCCAAAGCAAAGGTATATACACGTATAGGACTCAACATTTACAAATTCAA
TATTCTTATATGTGGAAAGCAGAGCGTTACGATTATCTCCCAGCTAACTGAAGCGATTGAATGTCTATACATAATTTGTA
ATGCAAAATAAAATAAAATATATCACGTTATATTAAACAGTAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 1 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)