FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\GH25653
Open Close
General Information
Symbol
Dmel\GH25653
Species
D. melanogaster
Name
GH25653
FlyBase ID
FBcl0121153
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG6340
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
  • chimeric clone
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,039
    NCBI
    AY051612
    Full Length sequence dataSequence Downloader
    ATCCAATGAGCGACTAGCTGAAAGAGTTGCCCTCAAATACAACAAAACAACAGAAACAAAACTTTCGCCTCGGACAGATC
GTCCAGTGGCTTGCTGGGCGGGGGGATCTTGTACTCGGTCTCGGGCGTCTCGTAGATCTTCTCCTCCTTGGGCTCCACAA
CGGACACATTGTCGGGCAGCGGCTTCTTGGGGCCGATCTTGTTCTTGGGGTCGTAGGGCAACATGACCTTGACCTTGTTT
TTTTTGGACGCACCTGCGTGCTTCAGCCACATTTTTGGCCTAAAACAATTTTTGGCCCCAAACAATGGAGGCCCTAGTGA
ATTATAGTAGTGAGGACGACCAGGAGGATGCTGGCTTTCAGATACGGGCACCACAACAACAACAACAACTGCGGCATTTG
CCGCATGCTAAATCCCCGAAAACAACAACTGATAGCTTATTAAAGCAGAAGAACAACAATAACTATGGCAGCGGCAACAG
TCCGCGCGGCAAGGAACGAGAAAGAGAGCGCGAGCGGGAGAGAGAGAAGACAAAGCCGATGTCGCAGCAGCAGAGTCATA
GGAGCAGCAGCAGAGGACGCATTGATGAGGAGCCGAAGCCCAGCAGTCGCCACCGCCAGGAGCGGGACAAGGACAGGGAA
CGGGACCGCGACCGCGAACGTGACAGGGACAAGGACAAGAAGCGCAGCGATTCAGAACGCAGTCGCGACAAAGGACGCGA
CAAGAATCACAGCAAGGGCGACCATCGCCACCATCGTACCAAAGACGGCGACAGCCACAGAGAGCGGGAGCGGGATCGTG
ACCGGGAGAGGGAGCGAGATCGCGACAAGGATCGGGACCGTAGGCGTTCGGAGAAGAGCAGTCGCCACCACAGTGGCAAG
GAACGCCATCATCATCATCACAACAACTCATCGTCAGCCGCCGCTGCAGCGGTCAGTGCAGCATCAGTCCGTCGATCCCA
TGATGAACGCAGTCGTCGTCGCCGGGACTCTCGATCCGGTTCCCGCACACCAACGGGCACGCCGCCCTCTCGTCGCCGCC
ATCAGTCGCAACAGTCACGCAAATATCGCGAACGCGACTCCAGCAGCCGCTCCAGATCTCCGAAGGAAGAACAAGCCACA
ACACTTCGCGGTGGAGCTGTGGCCGGATCTCATATGCTGCTCGCTAAATCAGGCGGCGGAGCAGGATCAACGGCAACTAA
TGCAACAGCAGCGGCGAGGGTTGCAGCGGCCGCTCTGGTAGCTGCCAATGCAACAGCCGCAGCCATGGCAGCCGGTTCAT
CCGGCGGCGGTGGCGGCGGTCTCCTGCCCACGCCCAACTATGCGCCCAAGATACCCTCGCTGATGTCCTTGGAGCTGAGC
ACACCAGCGGCAGCAGCACCAGTCGCAGAACCCATCCAGCTGCCCAGTTACTACAATCCGAGCATCATCAATGCCAATCG
CTATGCGGAGCAGCAGCAGAAGCGCAAGCTACTGTGGGGCTCCAAGAAGAGCGAGAACTCGGCGAACAAGTGGGGCAACG
CGCACTTCTCCCAGGACTCCGACGGCAAGGTGGCCTCCAAGTTTATGCGGCTCATGGGTATCAAGAACGCTGGTACGTCC
GGCAATGACGATGGCAAGGGCGGCCAGGCGGAGTCCACCAATGGAAGTGCAGCAGGCGAGGGAAACCGCGCAGTAAGAGG
TGCTGCGCCATCGGGAGCCGCTGGCGGAACGAAAGTGCCCACGGACGTGCAGCAGCGCCAGCGCATGTTCTCCAACATGG
AGCAGCAGTACGAGATGGCGCGAGCCGCCACACACACGATGCGTGGCGTGGGGCTTGGTTTCGGCTCCCAGCCACGTACT
TTCTAGAAGGGATCGGAACGGAGGAGGATCGGCTCCCTGCCAGGCTGTAAATACCATGCCACTCACTCAACTCATTAGTC
AGTCAATATGTTTCTCCTCAACAACTTTTTGTTTGTGTTGAGAAAATTTTTTGTTTTTTTGATTTCAAATAAACGTTGCA
ACAGAATTTATGAAAAAAAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)