FB2026_02 , released June 18, 2026
Clone: Dmel\GH03827
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General Information
Symbol
Dmel\GH03827
Species
D. melanogaster
Name
GH03827
FlyBase ID
FBcl0122709
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG9861
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI19406
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,842
    NCBI
    BT010111
    Full Length sequence dataSequence Downloader
    CTAAGATCCTGTTTTTTTTTTTTTATTAACAATTTTCTAGAAAATAAAATTATAGAGTAAAAAATTCCGCCACATAGTGA
ATAGCCATGAGTTTCCAGAATAAGCTGGATGAGTACACCAGGAACTTGGACCTGGCCAAGTGGTATATGGGAATCTCGGC
GAAACAGGAGGCCACGATGTCCACAATGTCCGAGAGCCTCCGGGATGACTTCGAGCAGGGAACAGGGGAAAAGATGTTCA
AGGCACTGATATCCCTGGGCCTCCAGCCGGTGATATCCAAAAAGAAGATCAGCCGGATCGTGCAGTTGAGCAGGAACAAC
ATACTGGCCTTCCTGTTCTTTGTGATGGAGGGCTACTACAAGACGAAACAAACGGATGGCGTTTACAGCATTAATGAGCA
GCTGCTAATGAATGCCATTGCCAAGATCGACATGTTGCCCACCATTCGTGCTCTGGACATTGTGCTGCCCCAGCCTCCGG
TCAAAAAACCGGATCCTCAAACTCAATCGTTAGCTTCCCTGACCACGGAGCGTCCACAAAAGAAACCCTCCAAGAAGTCG
CCCTACTTTCTAAGGCAACCGAAGCCGGTTCAAAGGACCTCCCGTCTGACAGCGAAGATTCCCGAGTTTGTCGTGTCATT
CAACTTTTGGCCCACCAATGGACCGCCCGAATATGGCACTGAGGAAGAGGAGCCTTGGTATGCGCTGTACCGACTGAATC
CCGGCCAGCGACTGATCAAGAAAACCCTCTCCGAGACCCTGGAGCGTTACTTTAAATTGGCAGCAGTTGAGGGGAAGCAG
GATGCGGAGGAAACCCAACAAAGTCGGGAGCCCGAAGCCAACATGTGCTTGGTGCACAAGGGTCAGGTGTTGCAGGCCCA
GCTCCTGAGGGATGAACTGGCGGTCAAGGCGAGGGATCGCTGCCTGGAACTCCTCGATGTGAACGAGCCATACGCCAAGC
TTCGAAAGGCCAGAATCGTGGCCCAGTTGGAGCACGACATCGACGTAATTGGCCCGCCACCGAAACGCGATGCACTGCGA
CCAGACCAAGGTGCTGACCATCGAGAATTTTGACTGCGTGCTGTGCCAGCAGATGCTGGTATCGCAACCCTGGCCGGAAA
CCAAGGGTCAGATTGGTCGGGCTCTGGTGGGCGAGGATTGTACCGTGGCTCATACTACTGCATTCGATACTTACCGCGGA
AAAAGGCTGGAGGGTGGTGCGGAAAAGCCGAAAAAAAAGGATGGCACAGTAGCCCGTACCAGTGGAATCGATACTTGTCG
CGGAAAAAGGCTGGAGGGAGGTGCAGAGAAGACGGATTCCACAGTACCCCAACCGCCTGCAGAAAAGGGCCGCGGAAAGA
GGCTAAAGGGTGGTGGAGGAAAGCCGAAAAAAGACAAGAAGGGGAAAAAGGAGAAAAAGGCTAAAGTAGTGGAACCGGAA
CCAGAAAATCCGAAGAAGCCGAAGGTATCGCCCTGGAAGCCACCGCCTCGCAAACTCGTTAGCGGCCCCTTCCGTATCCA
CAACATCGACTTCAACGAGTACACACAGGTGTGTACGCAAGTAGTTGGTCCTTCGGAGTGCGAGCTTCCTCCGGCTCCCA
AAAAGTCGGCCGTATCTTTTATCCTGGCCAAGGGAAAGATCGAAGGGAATGCACACAAGTGCGATATGCGCATGCCCAAT
AACAGGCATGTGAAAACTAGGTTCTTCCGGGGACCCCGTTCGAACAAGCCGTACCAGTTCAAATACCATCGGGTCTTCCA
ATCCGGCCAACCGAAACCCTTTGATCTCAGTCATATTATGGCCAAGTCTTTTGTGAAGGCGCTGGATCAGTCGGATGGGG
ACGAAGCCGATCCGTGTTACTTTAGGGAGCTTTTGTCGGAGATGGTGATTGAGGACGAGGCGCCTCCGGATTCCAAGTCT
GAAGGCCTTCCCGAAACCGAGCCGGAGAAGAAATCGAAGCAGGACAGCCAGCCCCCGTTGGAAATCTTTAGTGACCTGGA
TCAGACTAGAATCTCCGACAGTCGAGAGACCTTGGATCGTTCGAGCAACCACAGTGGTCGCAGCCATGTGAACTACAAGG
CGGAGATCGTGGATGCAGTAGTGAGATGTGCCAAGGCGGTTTGGCAGAAGCGTACCCTAATAAAGCGGGCCGAGATGGAG
CGCATGGGCAGAATGACGCCGCGCAAGGCACTGACCCATAAGGATACGGAATCATTCGATCCGAACGATGCCGCCCAGAT
GGACAAGTTGCTAAAGGATGGCATGCGAGTACTCAGGAAGGAGCCCCGCTATGTGCTGGCCTGTCTGCCAGACGCCCACA
AGCTGCCAGTGCTGCGGGAGTGGGTCAAGAGGAGGTACGGCAAAACCTACAGCCAGAAGGAGCTCGAGGAGAGTATAGCC
GAGTCGAGCAGGATATTCGAGCTGGTCACCATCCTCCAGAGCAGTCCCGCCAGTCCCGATCTTATGGGCATTGATCGCCT
GCCCAGATCCAAGGAGAACTTTAACTTCTATAAGCAGATTAAGAATACGGCTGCCCTGGCTAAGCAAACATATCACGATC
AGTTGAATGCCCGCTACCTAAGCATCGTGAGCTCCGCCTGGTACGCCATGGGCAACTATCTGGTGCCCGGAGGACCTCCT
CGGAAGACCTTTTTCGCTTACATAGCTTCCAATCCGCTGGAGATCATGCGTAACAAGACGTGGAATGGGGATTTCCGGAA
CTACCGCTCCATGCGCGACAAGAGAAAGGAGCAGGAGAAGCTTTTGGGCAAAAAGGACTAGATTACAATAAATTAAAACT
CTTTTTCAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)