FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\GH24360
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General Information
Symbol
Dmel\GH24360
Species
D. melanogaster
Name
GH24360
FlyBase ID
FBcl0125249
Feature type
cDNA_clone
Computed gene(s)
Dmel\CysRS-m
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,787
      NCBI
      AY058452
      Full Length sequence dataSequence Downloader
      ATGAAACTTTAGAAATAATTTTATTTAGACTTTAGACAAAATTTACAATATATAACAATGTATAGCCTCAAGGAGGGGCT
CCGGGCTCTTAACAGTCGAACGAGACCAGCTCTCATTTGCCGGCGCAACATTTCGGAGGAGGCTCCCAAACAGGATAGTC
CGTTTAAATGGAGAAAACCAATTGGCCAGCACACAGGAATCAATATATACAATCACGGCTTGCGGCAGAAGGTACCCCTA
ATCCTACGCAATCCCCAAATGGTCACATGGTACACTTGCGGACCCACTGTTTATGACTCCGCGCACCTAGGTCATGCCAG
CACCTATGTCAAGGTAGACATCCTGCAGCGCATTCTCCGCGATTATTTTAAAATCAACCTGGTGACAGCCATGAACATCA
CGGATGTTGACGACAAGATCATTAAGCGGGCCCAGCTCGCTGGACTGGACTGGCAGAAGATGGCGCGAGCTTATGAAGCG
GAATTTCGTCAGGACATGTTGCGTCTCAATGTTCAGGCGCCGGATGTGCGCAGTCATGTGACTACCACCATGCCTGTCAT
TATTCAGTTCATTCAGCAGCTGATAGACGGAAAGCAGGCTTACGTTACCCCGGATAACTCTGTTTACTTTGATGTCTCCA
AAAGCAAGAACTATGGAAAGCTTCAAAACTTAGGCCTAAGCGAGGACAAATTGGACCCCATCAAACGAAACACTGCCGAT
TTTGCCTTGTGGAAGGCGCGTAAAAGTGGCAGTGAGCCCACGTGGGCTGCACCCTGGGGTGGCGAGGGACGCCCCGGTTG
GCACATTGAATGCAGCGCCATTGCAGGTTTATTCTTTGGCCGCCAGTTGGACATTCATGCTGGCGGCTTGGACTTGCGTT
TTCCGCACCACGAAAACGAGGAGGCGCAGTGCTGTGCACGGTATAAAACTGACCAGTGGGTAAACTATTGGGTGCACACC
GGACAACTTCATATGGTTGGAGATCGGGAGAAGATGTCAAAGTCTCTGGGCAACACGATCTCCGTGTCGGAGCTGCTGAA
GAAGTACACCGCCGATGAGTTTCGAATGGCCTGCCTGCTGTCCAATTACCGCAATGCAATGCCTTATAGCGATCAGTTGA
TGGTGACGGCTCGCCAGACCCTGCAGCGGTTTAGAAACTTTCAGGCCGATCTAAGTGCCTATACCCAATTCCTGAAGCCC
GTGCACTTGTTAGACGAGGGAGCGTTAAAGGCCCAACTGACTCATACAGTAACCGAATTCGATAATTGCCTTCGAGATGA
TTTCGATACCGCCAGAGCCATCAGCGTGCTAATCGATCAGATGAGCAGCATAAGTCGCTGCATAAATGAGCAGCAAGTGG
ACGCACAGGAGGAGCCCGCCTACTGCATTGATCTGCTGTTGGCAGCTGGAAACTTCATAAACCGTGCCATAGTCACGTTT
GGTCTATCAGAGTTGCAGGATAAAGAATCGTTGCAAGAAAAGGTTTCCTTTACGGATCATAGCATTGATCCCAATTTGCT
TGTGAATGATGTGATAAACGTTCGGGGAAGAATGCGGGAAAGGGCCACCTCCGGAAATGTTAAGAACCCGCAGCTTTTAG
CCGCCTGTGATGAACTGAGGAGCTTGTTACAACAGCACGGCATTCAGGTCCGCGACCACAAACAGGGCAGCTCCTGGGTG
TTTGCCGTGTCCTGTGAAAAGCAAGATGATAAAAGCAAAAGCTCTGCCGAATAAAAGTTAATATATTATTCTTTGGATGG
TTACTACATAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)