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General Information
Symbol
Dmel\GH01154
Species
D. melanogaster
Name
GH01154
FlyBase ID
FBcl0127775
Feature type
Computed gene(s)
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI19449
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    5,366
    NCBI
    Full Length sequence dataSequence Downloader
    AGACGTCGAACATGTGAGGCTCAGGCGGCGTACACATATAATACTCTTTTCTTTTCGGTTTTTTTTTCAAATTATTATTT
    TTTTTGTTTCTTAATAAATTTAATACAATTTTTAACCGGAGCAACCAATGATACTAACTGAAAGTTCCTACGACTATGGT
    TTATGTTTTCCTCAGTGTCGCTGGCAATTATGTGTCGCTACCTGGCGCTGGAACTGGCCCCACTGAGCAGCCGACCATAC
    TACTGATCAACGGCATCGCCAGCGATTCCCAGCTAGAGGATAAGCAGTCCAAAGCAGCCGCCTTAAAGTTAGCTCTCGAT
    AACAACAACAGCGAGGCAACAGCACCAGCACCAGCCCAGCCACACCAAGTTCCCACCACTCCGGGTGGCTCGCATCTCCT
    GGACTTCGAGTCGCATCTCATCGAGAGCATTGCCGACGAGGCCAGTGGCGTTGGGATCAGGGAACTAACTCCGTTCGAGC
    AAGAATTGCAGCGGGGCACCTCCAAGGCGGGCACCAATGACACAGACCCAGTCATAGAGGTAGAACCAGCTGGTGTTAGG
    ACCAAACAGCCTGTGGAAATCTCACCGCCGGCACATCAAATCGCCGAGGTTGACGAGACGGCCACTCCGTCGGAGGAAGT
    ACGTCGCACTGCCGAGCAGCTGGTGGATGAGATCGAACAGGAGCTTGATCCAGGCACTCAGCCGGGATGTAGACGAGGCG
    CAGCGCGTGCACGAGGATCAGGTCCAGCGAGATCGCGACGAGATCAGCGCACTCACTCAACAGGTCGAAGTCCAGTTGAA
    TGAGCTGACCGGTATCCTTAAAAACAAGCCCCAGGCGGAGATTGTCTTGGAACTGCCGGCGGAGGAGGAGGAGGAGCAGA
    AGCCGAAGCCATTTGTCGCTGCTCCCAGTGAACTGAAGCTCGTGGAGGTACCCACTCCCAAAACTGAGGCTGAGGAACAG
    GAACGCAAGGAGTTCATCGACTCACTGCCCCAAATCGAGCAGAATCGCCTGCAGGGAGTCAGTGGATCCGAGGAGACGGA
    TGCTCAAAAACTATCGGCAGACTGCAAGAGGGAGTACTACCAATCGCTGAAGAAGTACCTCCTGCACAGCAGCCAGGAGA
    AGCCGCCAGTGCCACTGCAAACATATCGCTGGGAGGACCTCAAACGAGCCAAGGAACGGGGCGGCTATCCCTGGACACAT
    TTGTACAAACGCCCACTGGGACCCGATGAGCAGCCGGAAATTGTGCTGCTCCTGCGAAAGTCCCAGGAACTGCGTTTCAA
    GTCCGAGTCTCCCAAGTCCCTGAAAAAGGTACGCTACGACGAGCAGGTGTTGGTGAAGGAGACCGAACGCTACATACAGG
    ACCTTTCCGAGGACGAGGCGGTAGCCACAACCACCGACGACAGCAGCGAAGAGTCCTCCGATTCAGAGACCGAATCGGAA
    CGTGATCAGCACAACGAAGACGCTCTGAGTGAGTGCATCTCCTGTGTTTCCGACTCCGTGCTAGCGGTTGGAGGACACGC
    CCGACCACGTAAGACGAATCGGCTGGCCCACATCCGTGACCTCATACGGCGCAGGCGCAGCGGACGCACCCACGAGGACG
    CCCAGTCACTGCCCGGCAACTCCGCTAATCCCAGTCGACAGAGCAGCGTTCACGAACTAGCCCCGCCGCCGGGATCCCTA
    ATTCCCAACGCCGATAAGCCTCCGAAGTCCAGCAAACCCAAGCAAGGATTCGACATCATGAAGAAGCTGAAGAGTCTGGC
    CGAGCGCCAGAAGAAACGGCTGAACATTAAGAGGATCACCCTGAAGAAGGACGAGAAAATCGTTCTCGGCGAGCAGCAGA
    AGATTATGAAGCTAAAAGCCTCACCCAAGTCGGATCGCGGTGAGATTCCTCACTTCATCGAGAAGCAGGACTCCGACGAA
    ATCCTGGAACTGGTGGAGTACGATGAATCGCCATGCCGCAAGCGAACCAAGGAGGAGCTACTGGAAGATCAGCCCAGTGG
    CAGTGGAAGAGTGCCTGAACCCGATGAGATCATCGAGCTGCCTGTGGTCAAGACTGAGATAGAGGCTCCCACAGTGGAAG
    TCACCGAGCCTGCAGAGGAAAAAGTGGATCATAAGGAAGAAAAGGAATCGGAAGCAGAGGAGGACCCGCCCAAGAAAACA
    CCTCGCATTCGGCGGGAGCATGTATACGAAGAGATTGGCCAGGCTGGATCACAGGAGCTTGTGAATCAGCCAATCCTGGA
    GCTAGAATCTCTAAAGAAATCTCTGACGCGTCAGGACAATCTCGCCATCGATGAGATCGAGGCGGCCAAGGCAGTGCCGC
    TGGATCGCATGGGCAGCAGCGAGGAGGAGCAGGTGACCGCCGGCAAACCCGGTGCGCTATTTGCCCCCATCTCGTCCATA
    GACTCCACCTCGTCGGATGAAGATCGCGCCCGCCTGGCGCAACTTTCACCCGTTACCGAGGAGAGTGATGAACCCATGGA
    CATCAGTCCAGATCTGCGTCCATCCATCAAGAAGGAAGCTTCCCCAGCGCCCTCAGACAAAAAGGTCACCTTTTCTCACG
    TCGAAGACGAAGCGGAACCGCATCGTGAGGATGTCGAGCTGCCTGAGGATGTCCTGGAGGCGGCTACCAATGCCGCCAAA
    TGGAAAAACGAGAGTGATCATGAATACGAGCCCGTGGGCGTGACGCCGGCGCACGAATCCTCGCCAGCACCTAGCCCCCA
    AAGCGAACTGCAGCTGCCCATGGACATCGACTTACCGCTTAGTTCCGCCGGGACCACACCACGCACTGAATCCCGGACTG
    ATTATGAAATCCACACCAGTCAAGTGGACTCAAGCGCCTTGGAGGAGTTGCCGTTGCAGCCGGAGAATCGTCGCAAGGGT
    TTCATGGCTTCGGCACAGGATCGCACCAAGAAGATGCAGGATGGCATACGTTTGCAAGCTGGAAAATTACGCACACGACT
    CCAAACCAAGCCGAAACCAAAACCAGTTTCCGGGAGTCCAAAAGGTAAGGCCAAGGAGCGACGACGCTTTAAGGCTCCCG
    AGTTCTCGAAGATCAAGATGCCTGAGATTAGGCGACCGGATATGTCCAAGCTTAAAGAGCTAAAGCGACCCGAGTTCACC
    AAGTTTAACAAGCCGGACATGTCCAAGTTCAAGTTGCCAGAGAAGTTCTCCACTCTGAAGCTGCGTCGTAGCAAAAGTTT
    CAAGGAAAACGAAGGTGAAGTGGTTTCGGATGAAACCCCGGAAGGCACTGGAGGCACAGCGTCCCCAACTCAGCCGGCGC
    CAAAGAAGAAGTTCGAGTTCAACTTTGGCACCTACCCACGTGCTTTTCGTAAGAAGAAGCCGGTAGAGGAACCAGTTGCG
    GTGGCCACTGAGTCATCATTGGGAACTGAAGGCCTTAGTGTGATTCCCAGCACGGAGACGCAACCGTCGCAGACCTCTAC
    CAGCTCTCCGCAAGGTGATCGTGGACCCGGACCCGTTCGTTCCCGCTGGGCGGATAAGTTCTCCGATGTGAGCTACAACG
    ATAGCGAAGGATCACGTTACCGACGCTACGGCAGCGAACTAGAAAGCTTCGACAGAGAATCCTCTCTGGAGCGACGCATG
    AAGGAGGATCTGGAAGGCACCGAAGATACGGCCAGTGAGGCACAGCCCCAACAGGAGATGGGCATTTTGGGCGTGGTGGC
    CGATAACAAACAGTTTGCCGAATTCGACGAGGAAAACCGGGCCATTCACGAGATATCAAGTAAGAGATCCCGGGAGTTCA
    AGCGCCGCCCTATGGTTCATCAAGATTCTGACCTGCGTTCGGAGGATAGCAGAGATGCTGAAGGCTGGACGGAGAAGGAT
    ATACAGAAGAACAAGCTACTGAGGAAGGCGGAATTGGAGGCGGAGGCTGGCTTTTACAAGTTCCACGACTTACAGGATGC
    TCAATCCACAGCCAGTTCTGGCAAGAAAGTGGTCATGGAGACAATCGATGATGATGAGTTCTTCCTTCGCAAGCGTGGAA
    TTTCCGAAGATAACATCCAGTTGCGACAGTACATCAGTGAAGCTATTCGCGAGGGTTACGACATGCCCAATGCCCTTAAG
    CATGTGGGCTACTCAGCCGAGCAGGTTCCGGCGGAATATGGTGACTACGATGTGCCTCCGGCCAAGCCGCGTCGCCTCCA
    CCGAAACTACCAGCCTGACTTCGACTCGCAGGAGTTTCAACGCAGTGATTACGGCGACGATCTATCCATGTCACAAAATG
    GCAGTGAGTTCACTCCAAAGCGACCACTTCGTAAGGCTCGCAGTCGCAGCAAATACTCGATTGAGGGCAGCCAGGACATC
    CCCCAGGATGGTGGTAGCAGTATCCAGTACTTCGAAGATGACGAGGAGTACCTACGGCCGCCGGCCAGAGATCAACCGCT
    CACGGATTCCGAGCAGGCACTCAATAACCTCGATCTTGGCGGCAAGGCAGCGGCGATGATTCAGGAGGAGATGGAGCAGG
    AGCTACAGAATAAGCCCCGTCCGCAGGCACCGAGGCGCCAGAAGAAACGCACAAGGGACGACGCATCCGTGGACAAGGAT
    GCAGACTCCTTCATTAACGGCTTCGGTGGTAGATCAGTATCGAACACCTTTTTGCAGCCACACGAAGATGTAATTGTTTA
    TCGCACTGAACACGAATATCGTCACATACCGCTAGCCACGCCGGACAGATTTACGGATGCCACCTCTGCGCGCACACAGC
    GTTCCGAGGACGACCGCACTTCCCGTGGTGCCGACTCCCTGATTCTGGACGGGCACATAAAGTCCCGCGCCGAGCTGGAT
    GACAACGAAGATGTGGGACCACGGACAAGGAGCGATGAGAAGTTCGTCATCGATATGCTGGAGAGTGATGGCTATGCGGT
    GGTCCGCAAGGAACCGCTTCCGAAGCCAACGCCACCTGCCCGCCGGAAGAAGTTCATACGATCGCCGGGTGAGCGGTTCG
    CCACGTTGCCCAGCATTCGAGGCTCTCGGGGATCACCGCCACCCGAACGACCACCGCCACCACAGCAATACGTGCCCACT
    GAGGACTCTCCGGTGGTGCCACGCCGCAGATCCGCGGCCAGCCTGGACGAGATACCGCTGATGATCAAGTCGAAGTAAGA
    GTATAGCCTCTTGAGCTTTGACCAATTGAGTTGTTACATTTTTTTGGTTGCCGTTAACTTGTTTCGAATGCATTTTTGTT
    AAGTTTCTATTTAATATTCCTTTGTTGTTAAGTTTTAGACTAGGCTAGGCAATAAGCAACTAATCCGATCCTCCCGAGAC
    ACGCACTCCATACACACAATAAAGCCGATCACCACACAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    AAAAAA
    
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    Reported As
    Symbol Synonyms
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Crossreferences
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)