GAACTCAAGCGAGCCGAAACGAAACATCGCCAGATACAGATACAAATTCTCTGGTTCGCAGCATTGGTCTGGTCCATCGG CTCGTTCTCGTCCGTTTCATTTATTTGCGCGCGCGCGGTAGTATCAGACAATTGCTAAAGTTCCATTTGTAATATTAATC CAGTTGCGATTTCTAATTCGAATTTGAATACGTATTCATCCCAGTGCGCCAGTGTATTCCGTATCCTGACCAGCCATGTC GGCCAGATGGCGATAGCGATGCCGATGCCAAACGATCGGCGTGTTCTTGTGAACTCTGACATGGTGCTCAGTTGTGGGAC AACCTTCGCCACCGAAAAGTTTGCAAAAATAGCCGCTGGCCGAAAGCGAAAAGAAAGCCAGCAACTCAAATGCCAACGGT GGCCAAGTTAATGGTGCAGCTCGAAAGTGAATGCATTCGGCGCGATGTGTGAAGTTCAAGCTTAACATTGGCCAAAACCC AACCGCAACTGCGAATAAATTCTGTGATTCGGCAAAAAGATTCATCATGAGATCGTACAATGGCTCCAGTACTAAGTAGT TTATCTAGCTAATCGCAGCCATAAGTGTGAGTCGTTTTAATACCCACGAGCCGTAGTCTTTGCCTTTCTGTTCGTTCGTA TTTTCGCATTTTTTGGCGGGTGCGGTGCCCACGGCAACGGAACGAGACTGTCGCATTTCAATTAACAAAATAAGCAAAAA GCCAAAGAGCCAAAGAGCCATAGAGCCAACATAAGTGTAAAGATACATATATCTACAAGATATACGTATAGCCGTATATA CGCATACTGTGGATTTTGTTGCTCTTTCGCGCGCATTTTTGCACGTGCCATTTGGGATTTCGGATTTCTGAGATTGGAGA TCGCTATCTTATCTGGTCATCGCCATGTCCATGCCGGATGCCGGTTCACTTAAATCGCCCGCCGGCGGCCAGGAGGTCGA GTGCCATCGCCTGCTGTCGGACCTGCCCGCCTTCGCGTATCCCAGCACCACACTGCGGTCCACAACTTCTGGTCACAGCA CTGGCACGAATAGTACCACTGTCTGCGAATCTCCCGGTGCTCGGGATCGTACCTCCTTCGCGTGCTCCTCCTCTTGCGTA TCATCCGCCTGCTCAGCCACCGCCTCCGATGACGACGAGGATGAGCGTGCCCTTGAGCGCTGTCTGCGTGGGACGACGGA CGACCTTGGGGAGTCCGGCCTGCGGTATTCGCTGAAAGGACCACCGCCGGAGCTCTACTCGGCCAAGAAGGACTTCATCA GCAAGGGTCCGCTGCTGGGCAGACAGCTCGAGGTGAAGAAGAGGTGTGAATGGTGGTGCCACAAGTACATTCAAAAGATG TCCACCCACTGGCGGCAGAATGCCTGCCTGTATGCCTGCTGCATCGCTTTTCTGCTGGGCATGCTTATCATGTTCCATCT CGGTTTGCGAAGTGCCCATAAGGGCCAAGAGGAGCTGCCACAGAGCACGCACCCACTAGCCAACAGTCCACCTGCTACAC CAGCCACGCTCCATCCCAGAAGGCTTGATAACGACACGTCGACGGACCACGAGCCACCCGATGGCTTAGACGATCTGGAC GAGGAGGAGCACAGCGCCTTCGTGATGCCCACCAAGGTCTACAACTACAGTCTCAGCGAAGCTGATCTGATCTACGAGTC GAAACGAAACAGCGACATCAACAGTTTCCTAAAAGAGTCCGCATCGGCCTTTGCCATGACGGGAACGTATCGCAACATGT CCAATGAGATCTGGGACCCGCATCCGCAGTACAACCTCAATGTGTTCGGTCGCCAGTTGCATTTGGTGCTGCGACAGGAC GCCTCGTTCGTCCACAACCATTCGATGACCCACATTCGGATACTGAAGGAGGGGGAGGAGCATCCGGGACCGGAGACGGA GGCGGAGGCGGAGCAGCGCCACCTAGGCTGCTTCTACTCCGGTTACGTCGAGGACGATCCGCACTCGATGGTCTCCGTCT CACTGTGCGGCGGCATGACTGGTTATATCAAAACGAGTTTCGGCGCGCTGCTCATTCAGCCGGTGAACCGAACCAGCAGC GATGAGGTTCTCCACCGGGTCTTTCGCAAATCGCAGCGCAACGCCAGACACGCTGTCTCCAAGTTCGAGCTGGGCCTGGA TGATTTCATGAGCAAGCTGGAGCAGGTGCAGGAGGAGGAGCAGAAGTCCAAGTCCCGCAAACTGAATCGGAAGAAGCGAC ACTATGCGGACGTGGACAACCAGGTGTACACCTTGGAGGTGCTCATTGCGGTGGACAATAGCATGAAGCAATTCCACGGC GAGGACCTGCAACCATACATTCTCATCCTCATGTCGATCGTGTCCAGCATTTTTGCGGACGCCAGCATTGGTAACTCCAT TCGCATTTTGCTGGTGCGACTGATCAGCCTGCCGAATATCAACGACCAGACGCATTCCAGCAACGAAATGCTGAAGCACT TCTGCCAGTTTATTAACCAATCTGGATACGAAAGAGATACAGCCATGTTGATCACACGCGAACCCATCTGTGGCAGCGTG CCGGGCAAGATTTGCCATATGCTGGGCTTGGCCGAACTGGGCACTGTTTGCAGTTCCAGTTCCTGCTCCATTGTCCAGGA CACCGGACTGCCCACCGCCTTTACCATGGCCCACGAGCTGGGACACATCTTGAACATGAACCACGACGACGACGACAAGT GTATGCCCTATGTCACCAGGCAGAACAACAACAAGGTGCTCCACATCATGTCCAGCGTGATGGGCATCCACATGCATCCG TGGTCGTGGTCCAAGTGCTCCCGCCACTTTGTCTCCGAGTTTCTCGAGAAAACCGACAAGTCCTGTCTGGAGACCTCCGT GGGCGCCCACATTCCCTATGGCACCGAGCGTCTGCCTGGAGAGATCTACTCCCTGGACGCCCAGTGCCAGCTGAGTTTCG GCAATGACTTTGGCTACTGTCCCACGGACGAGGAGTGCAAGCGGTTGTGGTGCAACCGAACCTCCGGTAACTCCAACGAG CAGTGCGCCTCCAGCAATCTGCCATGGGCCGACGGAACGCCCTGCGGCTCCAGTGGCCACTGGTGTCAGCGGGGCAAGTG TGTATCCAACAAGCATGGATACGGACGCCAGGTGAACGGTGGATGGGGACCATGGACGCCATTCACGCCCTGCAGCCTCA CCTGCGGTGGCGGAGTGCAGGAGTCGCGTCGTGAGTGCAACCAACCGGTGCCGGAGAACGGTGGCAAGTACTGCACGGGA AGTCGGAAGAAGTACCGATCGTGCAACACACACCAGTGTCCGCCAGGCAGCATGGATCCGCGCGAGCAGCAGTGCTATGC GATGAACGGACGGAACATGAACATTCCGGGCGTGAACCCAGACACCAAGTGGGTGCCCAAATACGAAAAGGATGCGTGCA AGCTATTCTGCCGCATGGACATGAAGGTCACCTACTTCATGCTCAAGAGCATGGTCACCGACGGCACCTCCTGTGCGGTG GACAGCTTCGACAAGTGCGTCAACGGCATCTGCCGGCCGGCGGGATGCGACAACGAGCTCAACTCGATTGCCAAGCTGGA CAAGTGCGGCGTGTGCGAGGGTCGCAATGACACATGCCACGAGGTAACCGGAAATCTGCTCGTCTCCAATCTCCTGGGGC TAAATGATGGGAATGAGCCGAACAAGACACTTTACTATGTGACGCGAATACCAAAAGGTGCCTCCAACATAATAATCACT CAGCGTGGCTATCCCGACCAGAACTTCATAGTACTCACCGATGACCGGGACAACGAACTGCTTAATGGAAAATTCCTCAA GACCTATCCATTGAAATTCGTTTATGCCGGAGTCACGATGCAGTACACCGGTTCTAGTAGTGTGGTGGAGCAGGTGAATA CGACCTACTCATGGAAACTGTCACGTGATCTTATAGTACAGATCATTTCACTGGACGTGAGTCCGTCCAAGCGGCAGGAC ACCGTCCTCTTGTCCTACTCCTACACCATCGACAAGCCACCGGATTATGAGGCGGAGGTGGAGATCTATCGCTGGGAGAT GCAGGCCCCGAGCAACTGTGATTCCCTATGCGAGGGCAGGAGTCATCGACTGCCGGCCTGCATCAGCACCACCCAGGGTG TCAAGGTTGCCCCACAGTTCTGTGACAAGTCCGCCATGCCCAAAATCGATGATCGGGCTTGCAACACTGACTGCCGCCTC AATCTCACTGTGACGAGCATCTCGGAGTGCTCGGCCGCGTGTGGAGAATTGGGTACCCGGGAGAAGACCTACGCCTGCGT CCAGACATTCACGAATATGCAACGTTCCAATATTGTGGATATGTCATATTGCAAGCTGAAATTCGATGTGGCTTACCATG AGGAGTGTCGCGAAGGATGCTGGGTTTTATCGGAGTGGTCAACTTGCTCCAAGTCGTGTGGCACTGGTTCCCAGCAGCGT GAAGCTCACTGCTACCTACACAATTCCCGTGTCAGCGATGATCTGTGCAATCCACGGACCAAGCCCCATTTGAACACCCT AATCGGGATCTGCAACACTGAGTCGTGTCCCACGTACACGAAATCACCTAATGCCCTGGCAGTTAGTAACTGGGTGATTG GAGAATGGGGCGAATGCAATGAGTGGTGCGAGAAGACTCGTTCTGTGAGTTGTTCACATCCATACGGAATCGGCTGCGGC AGTAGAAAGCCCAAGGACGTCCGCAAATGCTGCCACATCAAGTACACCAGCGATTGGACAGACTGTTCGGTGCAATGTGG CGAGGGCGTCAAGAGGAAGAAGCAAAGCTGCACGCGGGTCTACAAGCCGGATGTTCCGGGTACCCGAAAGCGCCGAGTGT ACGTGGATGAATCGTACTGCATTAGCCGAAAGGTGCATCGTCCCAAGCTGCGCACCACCACCAAATCCTGCAGGATCAAC TGCAAGTGGAATGCCTCGGACTGGAGGCGCTGTCCTGCTGACTGCTCGGAGGAGTACCAAACGCGTGATGTGCGCTGCGA GTCCTTCCAGGGTGACGGAGTGGAGGACAAGCACTGCGATGCCAAGAAGCGTCCATCCAAGCGACGCATCTGCAACAACT GCGTGCGCCGGCAGTCGAGAGTCATCTCGCAGTGCAACTGTGAGGGTGTGGAGAAGAGGCGGGACTTCTGCTTCAACTCG CACAAGGGTCGCATCGCGTGTCCCACGAGAGCCAGGGTGGAGCGTCACAGCTGCACTCCGCCGCCACATTGCCGAAGGAG AAGTGCCATCGGGAGCAGCATCAGCAGCAGACCGAGGGGCACTGGAGTGAGTAGCAGCAGGAGCTTGAATTCAATCGGAG GCTCACGGAATCGCGGAACACCGAGAAGCTGTGCGGACCTGAAGGAGATGCACGGCTACAACAAGGATGGCAACTACCAG CTGGAGGTGAGATCGCGAATGGTGCACATCTACTGCCACGGGATGAACAGCAGGACGCCACAGGAGTACGTGAACGTGGA TCCGCAGGAGAACTACTCCATATACTACGAGTACAGGACGAAACAGACCAACAGCTGTCCGCCGGAGTCGCGAGGCCACG AGTACTACAACGATCAGAACTCCGGCCGGACGCACTTCAGAAAGCTGCGGCTGAATATCACCGATCTGCGCATCATGGAC AACGACTTCAAGTTCGCCGATTCGCGAGGATTGGCTCAGAAGCTGGGATCAGCGGGCGATTGCTACAATCGCATTGGCCA GTGTCCGCAGGGCGACTTCTCGATTAACATGAAGGACACGGACTTCTCCATACGACCGGGAACCGTGTGGCGGATGCATG GCCAGTACTCGGTCATGAAGCGTATCAGTGAGTTTGATACGACCACGCAGATGCGAAGGGGATTCTGCGGAGGATACTGC GGCGGTTGCTATATAGCGCCCGATTCTGGCCTTTACCTGGACGTGTTATGACTGAAACCACTGAGTAGTCAGCGGGAAAC TTTGTTTGACCTTCCCACGGTAATCGGGTCTACGTCCCGGGAAGGACCAAGGGTAAAGTTAATCCGAGCATCCCAGCATA TCAGGGTTACCCCAGCCAAGAATTACCACTACGGAGAGTCGCCGCTATCGATGGTTAAAAGTATTCTCGAATATTAGTTA GTTATATATAAACTGTAATAATCGTGTCGAAGTAGCCCGAACAAAACCATATCGTCTGTATATATATATAATTATATTTC GCTCGAAATTAGTAACTAGATATTATTGCCATGTAATGTATTAAGTATTGTAAATACTAGAGGATGTGAGCCTTTGAATT AGCAAATAGGGATTATCCATCATCATAGGTCGTCGCTCAGATAAGCCCGTATTTGTGCCAACTCCGGTATGCAGTGATGG AAGATGCAAAGATATCCTTTGCCTAGGTAAAGTCAGCAATGGGAACAATACCAAGAAGTGCATTTCAAGCAATAATTTAA TTTAATTAAAATATACTTACACTTTCCTCTGCTCCATCCCCAACAATTATTCCGATTATTCCCTGAAACAAGCCTAGGGC ATCCATCCATCCATCACTCACCGAAAACGAAGAGCTCGGCTAAACTAGTGCAATGCCGGTCTGTACAATAGTTGTATTAG CTTAGCTCCGCAATTAGTTAGTTAAGAGAGAGAGAGAGAAGTTGGGAGCAAATGTATGTGTAACTAAAACGAATACTCAT ATTATTTAGGATACCCCCCGATCGCACAAGCCAAACCAAACTATTCATCAAATCGAATTAACACTCTTGATTGATTGTTA TCACTGCCAGATTTTCAACGTTCAATCGATTGTATCCCAAATCGCCTAAGTCCCGGTACCAAGTATCCTCGCTGTATTTT CATTTAACTAGCTAGGCTAATGCAAAACCAATGTTAACAATTAATTTAATGTCAATTAAAAGGAATTTAAAGCTGTTACC AACCAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
