ATCGTCGGTCACTGCAGTAGCCATCTAGAAATCGTGCGGAACCAGTCAGTCTAGGAAGTTCAAACCGATCCTACACGGTC TACTACAATCCGATCCGATCCGGTCCGACCTCGTCTTCGAAACTGCCATCACAAGCTAAACAAGGGCACACGCAGAGTGA CAACTCCAGCGTGATTGCAGCTACAGCAACGGCAACAACCACCAGCAGCAAGCCTCCTATTGCTCCAGCAGCACCTCCTT CGGCGGTAAAAGCGAGTGACAAGCGATTCCCCAAGGCGTGCATCAAGAAGGTCCAGTTCTCCAGCGAGAGCCTCCGGTCC GACGTGGATGAGCTGTGCGACCAGCTGAAGGATAGCGCACTCAGCAATCTGAGCAACGACAACATTCGCATTGCCTGCCA CCAGAACAACAACAACAGCAGCATCAACAAGAACCAGAACAACAACAGCATCGACATCAGCATCAGCATCAGCAAGATGA GCGAAACAAATGAGTCCAATGACACGGCCGCCCAATCCGCCGAGGGCGAGCGGCCCAGCTTTTTGGTGGGTGATGAGATC GACGAGCGGGCCGCCGAGGCGGGCGAGGCATGTGACGAGTTCCCCCTGAAGATGCAGAACGATGTCCGCCAGAATGGCGA TATTTCCGAGCGGCGCAAGCGGTTAAGGCCGAGTATGTCGCGTGGCACAGGGCTGGGCCAGGACCGGCACCAGGATCGCG ACTTCCACATCGCAACCACCGAGGAGTTCGTGAAGCGCTTTGGCGGCACCCGAGTGATCAACAAGGTCCTGATTGCCAAC AACGGTATCGCGGCCGTCAAGTGCATGCGATCCATCCGGAGATGGGCGTACGAAATGTTTAAGAACGAGCGGGCCATTAG GTTTGTGGTGATGGTCACTCCGGAGGATCTAAAGGCGAATGCCGAATACATCAAGATGGCGGATCACTATGTGCCCGTGC CCGGCGGATCGAACAACAACAACTACGCCAATGTCGAGCTCATCGTGGACATTGCTCTTCGCACCCAAGTGCAGGCCGTG TGGGCTGGTTGGGGTCATGCCTCCGAGAACCCGAAGCTGCCGGAGCTGCTTCACAAAGAGGGTCTGGTGTTCCTTGGCCC TCCGGAACGTGCCATGTGGGCGCTGGGCGACAAGGTGGCCTCCTCTATTGTGGCCCAAACGGCCGAGATTCCCACCCTGC CGTGGTCCGGTTCGGACCTGAAGGCCCAGTACAGTGGCAAAAAGATCAAGATTTCCAGTGAGCTCTTCGCCCGAGGTTGT GTGACCAATGTGGAACAGGGTCTGGCCGCAGTTAACAAGATTGGCTTCCCCGTAATGATCAAGGCCTCGGAAGGAGGTGG TGGCAAGGGTATTCGCCGCGTGGACACCACTGAGGAGTTCCCCGGCCTGTTCCGCCAGGTTCAAGCTGAGGTGCCCGGCT CACCGATTTTCGTGATGAAGCTGGCCCGCGGAGCTCGCCACTTGGAGGTGCAACTGTTGGCAGATCAGTACGGCAATGCC ATTAGCTTGTTCGGCCGTGACTGCTCCATCCAGCGTCGTCATCAGAAAATTATTGAGGAAGCTCCTGCCATCGTGGCCCA GCCAGAGGTGTTCGAGGACATGGAGAAGGCCGCCGTGCGGTTGGCCAAGATGGTGGGTTACGTCAGCGCGGGAACCGTGG AGTACCTATATGATCCGGAGGGTCGCTACTTCTTCCTGGAGCTGAACCCACGTTTGCAGGTGGAGCATCCGTGTACGGAG ATGGTGGCCGATGTAAATCTTCCAGCTGCTCAGCTGCAGATTGGAATGGGAATTCCCCTTTACCGGCTCAAGGACATCCG TCTGCTGTACGGAGAGTCTCCCTGGGGCTCCTCAGTCATTGACTTCGAAAATCCACCGAACAAACCGCGTCCCTCCGGAC ATGTTATCGCTGCTCGTATCACCTCAGAGAACCCCGACGAGGGCTTTAAGCCCAGTTCTGGAACCGTTCAGGAGCTTAAC TTCCGGTCGAGCAAAAATGTGTGGGGCTACTTCAGTGTGGCTGCCAGTGGAGGATTGCACGAGTTCGCGGATTCACAGTT TGGGCATTGTTTCTCCTGGGGCGAGAACCGTCAACAGGCTCGAGAGAACCTGGTGATTGCCCTGAAGGAGCTGTCAATTC GAGGTGATTTCCGAACCACAGTGGAATACTTGATCACTCTGCTCGAAACGAATCGGTTCCTCGACAACAGCATCGACACC GCCTGGCTAGATGCCTTGATCGCAGAGCGTGTGCAATCCGAGAAGCCGGATATCCTGTTGGGCGTAATGTGCGGATCGCT GCACATCGCAGATCGTCAAATTACTGAGAGCTTTTCCAGCTTCCAAACCTCTCTGGAGAAAGGTCAGATCCAAGCAGCGA ACACGCTGACGAACGTGGTGGATGTTGAGCTAATCAACGATGGCATCCGTTACAAGGTGCAGGCCGCCAAGAGCGGAGCC AACTCGTACTTCCTGCTGATGAACAGCTCGTTTAAGGAGATCGAGGTGCACCGCCTCTCCGACGGAGGCTTGCTCATCTC TTTGGAGGGCGCCTCCTACACCACGTACATGAAGGAGGAGGTGGATCGCTACCGCATTGTGATTGGCAACCAGACATGTG TCTTTGAAAAGGAGAACGATCCATCGCTGTTGCGCAGTCCGTCTGCGGGAAAGCTCATCAACATGATTGTGGAAGATGGC GCTCATGTAAGCAAGGGCCAGGCCTATGCTGAGATTGAGGTGATGAAGATGGTGATGACCCTGACGTCCCAGGAGGCAGG CACAGTGACATTTGTGCGTCGACCAGGAGCTGTTCTAGATGCAGGATCCCTTTTGGGCCACTTGGAGCTGGACGATCCAT CGCTGGTGACGAAAGCGCAGCCCTTCAAGGGACAGTTCCTGCAGCCAGAGAACGCACCGGTACCCGAGAAACTAAACAGG GTGCACAATACTTACAAGAGTATCCTTGAAAACACACTGGCTGGTTACTGCCTGCCAGAACCGTTCAATGCACAGCGACT CAGAGACATCATCGAAAAATTCATGCAAAGCTTGCGTGATCCCTCGTTGCCGTTGTTGGAGCTGCAAGAAGTTATCGCCT CCATCTCTGGTCGCATACCCATATCCGTGGAGAAGAAGATCCGGAAACTGATGACGCTGTACGAGCGAAACATAACTAGT GTCCTGGCCCAATTCCCCTCGCAGCAGATCGCCAGTGTTATTGACAGCCATGCGGCCACGCTGCAGAAGCGCGCTGACCG TGATGTCTTCTTCCTGACCACCCAGAGCATTGTGCAGCTGGTGCAGCGCTATAGGAACGGAATCCGCGGCAGAATGAAGG CCGCCGTTCATGAGCTGTTGCGTCAGTACTACGATGTAGAGTCGCAGTTCCAGTATGGACACTACGACAAATGCGTGGGA CTGGTGCGAGAGCACAACAAGGACGACATGCAGACGGTGGTCAACACCATCTTCTCGCACTCTCAGGTGGCCAAGAAGAA TCTGCTGGTCACTCTGCTCATTGATCACCTGTGGGCCAACGAACCTGGACTAACGGACGAATTGGCCAACACGCTAAGTG AATTGACCTCTTTGAATCGAGCTGAGCACTCTAGGGTTGCCCTGCGGTCCCGCCAAGTTCTGATCGCTGCCCACCAGCCG GCTTATGAGCTGCGCCACAACCAAATGGAGTCGATCTTTCTCTCCGCCGTTGACATGTACGGTCATGACTTCCACCCCGA GAACCTGCAGCGCCTGATTCTGTCGGAGACCTCAATCTTTGACATCCTGCACGACTTCTTCTACCACTCTAACCGGGCAG TGTGCAATGCTGCTCTGGAAGTCTATGTGAGGAGAGCTTACACATCCTATGAGCTGACCTGCTTGCAGCATTTGGAACTC TCCGGTGGCCTGCCGCTGGTGCACTTCCAGTTCCTCCTCCCCACAGCTCACCCGAACAGACTGTTCTCGCGCATGTCCTC CCCCGATGGATTGGATCAGGCAGCGGCAGAGTCTTTGGGAAACTCATTCGTGCGCACCGGAGCGATTGCAGCCTTTGACT CCTTCGAACACTTTGAGATGTACTCGGACGAGATTCTGGATCTGCTCGAAGACTTCGTCTCGCCAGCCATGGTTAATGCC AAGGTCCTGGAAGCCGTAGAGGCAGCGGATTCTATCTCGGACAGCCGACACAGCACCTCGATCAATGTGTCGTTGTCGGA TCCCGTAACCCGGGCGAATGCTGCCGAGGAGGCCAAGTCCACCGAACCGATTCACATTGTTAGTGTGGCTGTGAGAGAAA CGGGGGAGTTGGATGACCTGCAAATGGCCCAAATCTTTGGAAATTATTGCCAAGAGCATAACGAGGAGCTCTTCCAGCGA CGCATTCGTAGGATTACATTTGCTGCTCTGAAGAAGCGGCAATTCCCCAAGTTCTTTACGTTCAGAGCCAGAGATAAGTT CACGGAGGATCGTATTTACCGGCATCTGGAGCCAGCATCTGCTTTCCATCTGGAGCTGAACCGCATGAAGACGTACGATC TGGAGGCTCTGCCCACGGCTAACCAAAAGATGCACCTGTACCTTGGCAAGGCCAAGGTTTCGAAAGGTCAAGAGGTCACG GACTACCGCTTCTTCATTCGCTCGATCATCCGTCATTCGGATCTGATTACCAAGGAAGCCTCTTTCGAGTATCTGCAAAA CGAAGGAGAGCGTGTGCTCCTGGAGGCCATGGATGAGCTGGAGGTGGCATTCTCGCATCCGCACGCCAAACGCACGGACT GCAACCACATCTTCCTGAACTTTGTGCCCACCGTCATCATGGATCCGGCTAAGATCGAGGAATCTGTAACAAAGATGATT ATGCGATATGGTCCACGTCTTTGGAAGCTGCGTGTACTGCAGGCTGAGCTCAAGATGGTCATCCGCCAGTCACCACAGTC ACCCACTCAGGCAGTGCGTCTGTGCATTGCAAATGACTCCGGCTACTTCCTGGATATTTCGATGTATACCGAACAAACAG AACCAGAGACAGGAATCATTAAGTTTAAGGCCTACGGTGAGAAGCAGGGATCTCTGCACGGACATCCCATTTCGACGCCC TACATGACCAAGGACTTCCTGCAGCAGAAACGTTTCCAGGCGCAGTCCAATGGTACCACCTATGTCTATGATGTGCCCGA CATGTTCCGCCAGATGACCGAGCGTCACTGGAGAGAATTCTCCAAGGCTCGTCCCACCGTGGACATTCGCACTCCCGACA AGATTTTGATCGAGTGCAAGGAGCTGGTCCTCGAGGGCGACAACCTTGTAGAGATGCAGCGTCTGCCCGGCGAAAACAAT TGCGGCATGGTGGCTTGGCGCATTGTCTTGGCTACTCCGGAATATCCGAATGGCCGCGAGATCATTGTTATAGCCAACGA TCTCACCTACTTGATTGGTTCCTTTGGAATTAAGGAGGACGTTCTCTTTGCCAAGGCTTCCCAATTGGCTCGCCAACTCA AAGTACCGAGGATATACATCTCCGTTAACAGCGGTGCCCGCATAGGACTTGCTGAGGAGGTTAAAGCTATGTTCAAGATC GCATGGGAGGATCCAGAGGAGCCAGATAAGGGCTTCAAGTACCTCTACTTGAGCACCGAGGACTACGCCCAGGTGGCCAA CCTGAACTCGGTGAGGGCTATCCTGATCGAGGACGAGGGCGAGCAGCGTTACAAGATTACCGACATCATCGGCAAGGACG ATGGTCTGGGCGTGGAGAATCTGCGTTACGCCGGCTTGATTGCCGGTGAAACGTCGCAGGCCTACGAGGAGATTGTTACT ATCGCTATGGTTACCTGCCGTACCATTGGCATTGGATCCTATGTGGTGCGCCTGGGTCAGCGCGTTATCCAGATCGATAA TTCACACATTATACTCACTGGCTATGCTGCGCTTAACAAGCTGCTTGGACGCAAGGTGTATGCCTCTAATAATCAGTTGG GTGGCACACAGATCATGTTTAACAACGGAGTCACCCACAAAACAGAGGCCATCGACTTGGACGGTGTCTACACCATCCTC GACTGGCTCTCGTACATCCCCGCGTACATCGGTTGTGACCTGCCCATTGTTTTGCCCAACGATCGTATCGAACGCCCTGT CGACTTCATGCCCACCAAGTCGCCCTACGATCCGCGCTGGATGCTGGGTGGCCGTGTGAATCCCGTGAACGCTAATGACT GGGAGAACGGATTCTTTGATCGCGACTCGTGGAGCGAAATCATGGCCTCGTGGGCCAAGACAGTGGTCACTGGTCGCGCA CGTCTAGGCGGTGTCCCCGTGGGCGTAATAGCCGTTGAGACCCGCACCGTAGAAGTGGAGATGCCCGCCGATCCTGCCAA TCTCGATTCGGAAGCCAAGACCCTGCAGCAGGCAGGTCAGGTGTGGTACCCCGACTCCTCGTACAAAACGGCACAAGCGA TCAAAGATTTTGGACGAGAGGAGTTGCCGCTGATTGTTTTCGCAAATTGGCGAGGCTTCTCCGGTGGCATGAAGGACATG TACGAGCAAATCGTCAAGTTCGGAGCATACATTGTCGACGGCCTGCGGGAGTACAAGAAGCCTGTGCTCATCTACCTGCC GCCCAACGCCGAGCTGCGAGGTGGAGCCTGGGCCGTGTTGGATTCCCTCATTAACCCGCGCTACATGGAAACGTATGCCG ATCCGGAGGCCAGAGGAGGAGTTCTCGAGCCGGAGGGCATTGTGGAAATAAAGTACAAAGAGAAGGACCTGGTCAAGACG ATTCACCGCTTGGATCCGACCACCATTGCGCTGAAAAAGGAGCTCGATGAGGCAAATGCGTCTGGCGACAAGGTCAGGGC TGCTCAGGTGGACGAAAAGATCAAGGCCCGCATCGCTGTGCTAATGCACGTCTACCACACGGTAGCAGTTCACTTTGCCG ACCTGCACGACACGCCGGAGCGAATGCTAGAGAAGGAGTGTATCAGTGAGATTGTGCCTTGGCGCGATTCCCGCCGCTGG CTGTACTGGCGTCTGCGACGTCTCCTGTTGGAGGACGCATATATTAAGAAGATCCTGCGCGCTCAGGACAACCTCTCCGT GGGTCAGGCCAAGCAGATGCTGCGTCGATGGCTGGTAGAGGAGAAGGGTGCCACAGAGGCTTATCTGTGGGACAAAAACG AGGAGATGGTGTCTTGGTATGAGGAGCAGATCAATGCCGAATCTATTGTTTCCCGCAACGTGAACTCCGTGAGACGGGAT GCCATTATTTCTACCATTTCGAAAATGCTCGAGGACTGTCCCGACGTAGCGCTGGACGCTGTTGTGGGTCTTTGCCAAGG TCTGACGCCAGTGAATCGAGGCGTGGTCGTACGCACATTAGCCCAGATGCAGCTGAATGAGGAGACCTCTAACAGCAACC AGGGATGATTCTACGCTATGTTCTAAGTTCTTTTTAGGCCCTCTATTTCATCCTTCCCGTTCCCGCCCTTCACCACATTT CTCAGTCCCGCTTTGCGCTTGTAAGATATCGCAAGGCGTGCATGATGTGCATTTTGTTTAGTTAAATTTGTTTAAAAGTA ATTAGCGTAAATCTAGTTATGTATTGGAGTTGTAGGCATGGGTTAATTTGTTGCCTAAGTGGCCCGATAAACTAGTTGTA TCGTGTACATATTTGATTATGTATATCCCCTGAATACAAATCATTCCATTAGTTGTATAAGAACTAAAGTCCTTTGTACA CAGAGCCTTTTGAATAATACGAGTTACTGAAAAAATAAATGTATGGTACACTACATTAACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
