FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\GH25238
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General Information
Symbol
Dmel\GH25238
Species
D. melanogaster
Name
GH25238
FlyBase ID
FBcl0131685
Feature type
cDNA_clone
Computed gene(s)
Dmel\tkv
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,614
      NCBI
      AY060782
      Full Length sequence dataSequence Downloader
      CTTCGCGAGTCGTGACGTATCCACCGGATTGGAAAAATAAACCATATTTTGAGATACAATCCAAAGAATCGTCGATTTTG
TTTACGCGTGAAATATTTTCGAGTTTCAACCAAAAACCTGTGTGCCTAAGCTGAAATATTGTTAACTAATGAAAAACGAA
TAATAAAAGTGCATCAACGGCAATCTATAGTTAATGATTTAAATGGGAAATTGCACATATATTTCTATGCTTAAACAAGT
GCCAGTGCGAATAAACAAAATAGTGTAAACACTGGGGAAAAACAACCAGAAACACACAGGATATGCCTTAACTGCGGATT
GAGTTTCACCTGATTTGTATTGCCACCTTTCGTATATAATTATAATCAACGTGATCACGCGCTCACACCAAGCAAAAGCA
AAAGCACATCGGCAGCAGAGCACCAACGCCACCACCGCCAGCAACAGCAGCAGGATCAAGTTTTTGGCGAAACCCATAAT
TTCGCTCATCTTCCTGCTCCTCTTCTTCTTCTTTTTCTTCGCGGGCTCCATGGTGTCGCCGGCTTGACCGCCGCCGCCTG
CCTATAAACAAACCCAAGAGGAGTCCAACCCCAACTCCAGCTCCAACTTCGACTGCGACGGCGACTCCATAACAACGCGA
AGACTCGACGAGATACAACGAATCCAGTTCTCCTGGTGCTTTTGTTTTGCTTTCGTTGCTGCTGGCTGCCTCGCGTTTAT
TGTTTTGCTGCATAGCCCGGAAAAAATAAACGTGGCAACGAGGGGATATCGAAGGGGAAACTGGCCCCAAAACACCCGGG
ATCCCGATACCCTCTCTATATAGAGAGGAAGGCCAACAAAAACAACCAAGAACGATCGGTTGGGGCAACAACAACTTAGT
TGCATTGCAGTTGTTGCCGCCGTATAAAAATCATCAGCCTTTGGCCCACGTCGTTGCTGTTGTTGTTGTTGCTCTTGTTG
TAGCCATCAGACATGGCGCCGAAATCCAGAAAGAAGAAGGCTCATGCCCGCTCCCTAACCTGCTACTGCGATGGCAGTTG
TCCGGACAATGTAAGCAATGGAACCTGCGAGACCAGACCCGGTGGCAGTTGCTTCAGCGCAGTCCAACAGCTTTACGATG
AGACGACCGGGATGTACGAGGAGGAGCGTACATATGGATGCATGCCTCCCGAAGACAACGGTGGTTTTCTCATGTGCAAG
GTAGCCGCTGTACCCCACCTGCATGGCAAGAACATTGTCTGCTGCGACAAGGAGGACTTCTGCAACCGTGACCTGTACCC
CACCTACACACCCAAGCTGACCACACCAGCGCCGGATTTGCCCGTGAGCAGCGAGTCCCTACACACGCTGGCCGTCTTTG
GCTCCATCATCATCTCCCTGTCCGTGTTTATGCTGATCGTGGCTAGCTTATGTTTCACCTACAAGCGACGCGAGAAGCTG
CGCAAGCAGCCACGTCTCATCAACTCAATGTGCAACTCACAGCTGTCGCCTTTGTCACAACTGGTGGAACAGAGTTCGGG
CTCCGGATCGGGATTACCATTGCTGGTGCAAAGAACCATTGCCAAGCAGATTCAGATGGTGCGACTGGTGGGCAAAGGAC
GATATGGCGAGGTCTGGCTGGCCAAATGGCGCGATGAGCGGGTGGCCGTCAAGACCTTCTTTACGACCGAAGAGGCTTCT
TGGTTCCGCGAGACTGAAATCTATCAGACAGTGCTGATGCGACACGACAATATCTTGGGCTTCATTGCCGCCGATATCAA
GGGTAATGGTAGCTGGACACAGATGTTGCTGATCACCGACTACCACGAGATGGGCAGCCTACACGATTACCTCTCAATGT
CGGTGATCAATCCGCAGAAGCTGCAATTGCTGGCGTTTTCGCTGGCCTCCGGATTGGCCCACCTGCACGACGAGATTTTC
GGAACCCCTGGCAAACCAGCTATCGCTCATCGCGATATCAAGAGCAAGAACATTTTGGTCAAGCGGAATGGGCAGTGCGC
TATTGCTGACTTCGGGCTGGCAGTGAAGTACAACTCGGAACTGGATGTCATTCACATTGCACAGAATCCACGTGTCGGCA
CTCGACGCTACATGGCTCCAGAAGTATTGAGTCAGCAGCTGGATCCCAAGCAGTTTGAAGAGTTCAAACGGGCTGATATG
TATTCAGTGGGTCTCGTTCTGTGGGAGATGACCCGTCGCTGCTACACACCCGTATCGGGCACCAAGACGACCACCTGCGA
GGACTACGCCCTGCCCTATCACGATGTGGTGCCCTCGGATCCCACGTTCGAGGACATGCACGCTGTTGTGTGCGTAAAGG
GTTTCCGGCCGCCGATACCATCACGCTGGCAGGAGGATGATGTACTCGCCACCGTATCCAAGATCATGCAGGAGTGCTGG
CACCCGAATCCCACCGTTCGGCTGACTGCCCTGCGCGTAAAGAAGACGCTGGGGCGACTGGAAACAGACTGTCTAATCGA
TGTGCCCATTAAGATTGTCTAAGCAGCTGTTTGTAGTCTCGTTTTAGGTTTAACCTAAGTTGCATTTAGTTTTCTGTTTT
TGTTCAACTTCTGTTGAATACGCAGTTCCGACCTTTTGTCCGGAAATTCATAGTTAGGTTCTAATCGTAAGTTGCTTGTA
CGTGAGCGGAGTAAAGCAGAGGATCCTTCAGAGGTGCCTATACTACGGTTACTTAAACCGTCGCACTGATTGTAAGCTAG
AGTTTAGATATAAGTTGGCAGGAGCTAGTTACTAAGGCCCCTTGCAAAATCTTTGGTTAGAAAAATGCTTTAACTATTTG
AGAAATATAGGGAGTATTTTGGATGACACCTATTCATGTCGATGTACATAAATATGAGATCAAAACAAAAAATCAATCGA
AAGAATCGAAATTTATATGTAACATACATTAATTTTTGTGCCAGCTTTTGTACGCTTTAACTAAACGAGTTAAGTCGCCC
ATTCTTTGACCCCCGAGGCTTTAGCCTCAACATAGAACATGTCCTTATATGTTTTGTTTGTATCTAGTGTTTAAGTGTAA
ACTTTGCAGACTTTGTGCAAGTAAATGCTGCCTTTTGTAAAATCCACGGAATTAGTTGCCTTAAGACAAAGTGTAAAGAC
AGGAGCCAACAGACAGGAGCCCGACTCCCTTCCTCCCCTTTTAGTAGCACATATTTTTGTATTTATAACCAGAGATATAT
AGAACTGGCAGTTGCGTATCTCGAGAAGTGCACTTGTGTCGGCTCTTTTGTTTTGTGTAAATAGCAAAGATGTTTATAGG
CCGCTAACCATACATATATATAGTATATATATTATATATTATCGTTATAAGCGATTCTGTAAATACTATATATGTATCTT
TATCTGTAACCGTTTAACTTATATCTTATCGTTCTTATCATGTAACCTTGTTTAAGCTAGAGTTAGATGACTAGCCTGTA
AGAGCAACAAGTAAACTATATGAACTAGATGGATATGTATCTTTCAGTTTTTTTTCTGTTTCATTATTACTGCTCGCAGA
CCACAAGAACTAAAGTTTTGTATTAAAGCGGTTAGTTAATTTATTTAAAAATAATAAAAAATTATAATTAAACCCAAAAA
AAAAAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)