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FB2026_02
,
released June 18, 2026
GTGAATGCCATGGCCGATACGCTCAACATCATTTGGCAGGACATACTGAATCTTCTGCAGGATCGTCAACACCTTCTGAT ACTATGCACACAGTTCCACGACAAGATGACGCAGTGCTTCAGGAAGATGGACCAACTGGAATTGGCCTGCGAGGAAACAC TCCATCCACCGGATGTGCCCCGTGTCCAGGAATTCCTGAACAGATTCAAGCAGCTGAGAATAGACATGCTCACCGGGGTA ATGGCAGCCCTGAAAGATGGCAACGAGCTGCTGGCCCAACTGGAGGAACTGGAAAAGCTGGAGACCCTCGACACAAGACC GGAGCACATTAAACGAGATGCTACGAGGGCAGTTCACCAAGTTCAGCAGTGGTTGGAGGCCCTGCATGACCGCAGAAACT CCCTGGAACTCGCCTGGCAGACGAGGAAAATCCAAATGGAGCAGTGTCTGGCATTGGCTTTGCTGGGCAGAGAATTAGTC GACCTGGAGGCAGCTCTCCAGCAAGCCAGAATGGAGCTGAATACCATGTACAGCCTGGGCGAGTGTGAGCACACGGCCAA CGAAATGCTCACCAAATACAGGGAGTGGAAACAGCAGGCCCTGCTCCTCCGGGATCGAGCTCTTAAGATCACGCGAGCCA AGGAGAAGGTTCAATCCGCTGGTCACTTCACCGAAGAAGACGCATGTGCTAGGGCTTATGCGGTCTTGAGTGGCTGCACA GAGCACTTGGATTTGGTGGATCAGCGGGAACACTGGCTGCATCAGTCCCGGGAGTTTTTCGCCAAGGCTGAGCACACTGT AAGTGTCCTGGAGAAACTAGAACTGGAGCTGACCAGCGTAAAGCTGCCCCCTCATTCACCGGAGAGTTATGCCATGTTCT CCAAAGTGGATCGAGATGTTCGCAACTTTACCGAGGAACCTTTGCGCTTGGGTTACGGCATTCTCGACGAAGTGGGACGC ACTCAGCCGGAGACTCAAGGTGTGAAGAGAGTCCTTGATGAGTTGGAAAACAGAAAGGTTTACATTCAAGGCATCTGTGC CAACAGCAGTGAGGATCAGCAGAAGGTTCAGCGAGCACTGAGTGAATTCCTGAACCATCACAATGAGCTTTTGGCCTGGC TGAGAGCATCCGGCCAACATCAATTGCAGCAAAGCGTGGATATGGGCGGAAATCTCCAGCAGGCCAAGCAGTTCCTTCTC CAGCACCATGAACTCATGCAGGATTTGGAGATAAAAGGCGAACTGATCAACCTGCTGCTCGAATCCATCAAGGTCCATCT GGAGTCCCTGAGTCCCCAAGAGCGCTACGATGTGGACTCCAAGGCGGAATCCTTGCACAAGCACTGGATCGAACTTAAGG ACCTGGTTCTGAAGCGTGTGGATTATGTGTCCCTGCTGATAGACTTCTTTGAGCTGGCCAACGAGCTCTCCAGCCAGCTG GACAACTTGCAGCGACAGCTGCAGCAAACTCCAGACGAGCACAAGCTGCAATTCCTCCAGGCCACTTGGACGGGCATCGC ATCCACCTTCGGCGAACTGAAGTCCCGCGGACAGCGATTCATCAACCTAAAAATTGTGGATCCATATCTCGAGACCAAAT CCTCGGCACAGGCGGTCCAAGAGACGCTGAACGACTTCAGTAAGCGGCAGGTCGACGTGACGAGCAGTTTGGAGAATTGG ACAACGAGCATTGCGGAGAAGCGAGAAGTCGAATACCTACTCGAGAAAGTCATGAGCGACAACGAGGAGACCGTGGCGAA GAGCACCCAGGTGGACACACAGTTGTATCCCGTATTCACGTCCCAGAGCGTGGACTCCAAGCAGCTGCTGATCAGCACCC GCGAGAAGCTGACCAACGTGATCCAGGATATCGAAAGGGCCCAAGATGAGATACAGCAGCGCATCCAGACCACGCTCGGC ATCCAGACCAAGGATCAGCCATCGCTGGCCAAGATCGAGCAGGTGATCAACAACCTGCGCATGCTGAAGGCCAAGTTGGA CGGCATCAAGTACGACTATCGCACTTTGGTGGAGAGCGTAATTCAGTTCCTGGAGAACATAGTGCAGCTGCGGCGCGAAA TCGACGACTACTTCGCCAGGCAACAGAAGGAACCCGCATCCGGCGCGGATCGCAGCATCGCGGAGCACGAGAAATTCCGC GATCAGTGCATGGATAAATTTAGATCGCTAATCACACAATCCGAACTGCTGATCGATCGGGTGCGCGTACTGGAACCGCC GGGAGCCCGCGAAATCGACACCGACCGCATACTGAAGCTGCTCGAGAACCTGCGCCTGCACTTCGAGTCCAACAGCAGTG CACGCATGTCCACCCTGGAGCGTCTGGAGAAGATCGAGCAGTTCCGCTCCGATCTCGAGGACATCGATCGCAGCCTGGAC AGCGTCAGCCAGCAGCTGCACGAGATCAACAACCAGAGCGTCGACAGTCTGGCGGCGGCCAAGACCACGTCGCTGGCGTT TGAGTATTTTGAACGGACCATAGAGCTGCTCGAGAAGCGGATCGAGAAGTTTACGGAGTCCACGAGCCAACAGCTTTTTA TTACCAATCCCGAGAGCGAGCGGTACGTCAAGGACGAGCTGCGTAAACTCAACGAAAAATGGCAGAGCTTCAAGGATCAA GTGAAGCAGAAACGAAAGAGCCTCAACCAGGCGACCGATTTCTTCGAGGTGGTCGAAAAGATCGACGCGGAGTACCGTGA GATTAGCTACTTTTACACCAGCGTGTCCAACAAGGTTCCATATCTGCGCGACTCCGTGGAAGCAGGAAATTTGGTCAATG ATATCGAAAACTATGTGACCAGTCGGGAGGCGGCTCTGCGCTCCAAGTTGGACAGTGCCTCGCAATGTGCCCACGATATG AACAAGGTGTCTTCCTTGTACAACGATGTAATGAACATCTTCCAGTCCTTCATCAAGCTCAAGATGGACATTAATGTGGT GCAGGAAAGGCTGAAGCAGGAGCAGCGCCAGAAGGAGCAAAGGGAGCGGGATGCTCGTGATCAAGCCGAACGGGAAAAGG CTATAAAGGAAGCAGAGGCCAAGGAAAGGTTGCACAGAGAAGAGCAATCCCGACTAGAAAACCAGCGCCAGCAGGCGGCC ATTGAGCAGGCTCAACGGGAATTGGCAGCCAGAGAATTGGCTCTGAGGGAGCAAGCTGTCCGGGAGGAGGAGGCCAGATT GCAGGCCATTCGGGAGCAGGCCACGCGGGAACAATTGGCCAGGGAACAGGCTGCCCGGGAGGAGGAGCTGCGTATACAAT CGCTCAGAGATATTGCCCGCAGGGAAGAGGAAGTGCGTCTCCAAAACATCAGGGATGAAGAAACCCGCATTCGCCGCGAG GAGGAGGAGCGTATCCGTAGAGAAAACGAGTCACGATCGAAACGAGAGGAGGAAGCCCGCATCCAGCGTGAGGAGATCAC CAGACTCCAGACCCTACGCGATCAAGTGGACCAGCAGCGAATTGTAACCGAAAACATCCGAAAAGACATTCAGGTTAACT CCATTTTCACGGAGCTGCGATATGCCTCTCCGCTTTTCACTCGGCCTCTGAAAGATGCAGTTAGCCGCGAAGGGGACCGA TTTGTCTTCGAATGCGAGGTTACGGGAACTCCAGAACCCGCCGTGGAATGGTTCAAGGATGGCATCAGTATCCAAACTAA TTCCGATTATAAGACCACCTTCGATAAGGGTATCTGCAGATTGGTGATCGAGGAGACCTTTGCCGCAGATTCAGCACGCT TCAGTTGCCGTGCTTCGAATCTGGTGGGTACATGCGATACTAATGCCACTTTATCCGTTCGGGAAAATGCCGCCGAAGTG CAGTTGGTTCCACCACGGATCCTACGATTCTTGCAGAGCGGCAAGGCCACTGAGGGAAGCTCCTTTCAGTTTGCATGCGT GGTGGCTGGAGTTCCCTTGCCCACTGTTCAGTGGTTCAAAAATGACAAGTGCATCGACGATTCTCCCGATTACGTGATCA GCTACAATAATGGAGAGGCCACTCTGAAGTTCGAGGAGGTCTTCTTGGAGGATGACGCGGTGTACACTTGCAGTGCCTCC AATCCGGCGGGCATTGAACATTGTTCGGCTTCTCTGATTGTGGAACCTCTGGAACCCACAGAGTTACCCAGTTTCAAGGT TCCCCTTTCGAATGCCATGGCTCGTGTGGGTCAGAAAATCAAGCTGGAGGCCATCGTTGGCGGAATTCCCAGGCCAGAAG TCTATTGGCTGCACAATGGCAAACCCTTCCAGCCGCGCGATTCCAAGTACGAATACGGCCGCGTAACGCTGATAATCCCG CAGGCTTATCCCAACGACGCCGGATCCTACGTCCTGAGCGCCAAGAACCTGGCTGGCGAAGCCTATACCAGTTGCAACGT GATAGTAAAGGGTCGACTGCCCAACGAGACCTCCGATTCCGAAATGGCCAGCGATATAGAACCGATCAAGCCCGCCGTTC ACTTGCCCCTCAAGGATGTTTCCATATTCGAGGGTAAGCCAGTGAGGCTGGACTGCGTGATTGTGGGTCAGCCCGAGCCC GAGGTCATCTGGTATCACAATGAGCGACCTGTCAAGGAGTCTGCCGATGTACAACTGCTGTTCCAAGGAGACAGATGCTC CCTCATCATTCAAGAGGTGTACCAAGAAGACGCAGGACACTACAAAGTGGTGGCCATTAATTCGGCTGGCGAAGCTTCGA GTAGTTGTGAACTCAAGGTTACTCCTCTGAATCAAGCGGAACCTGCCACTCGGGCGCAGGCGGAGAGGCAATCTCTACCG AAAGATTCGCAGCCCAAGTTCGAGAGACTTCTATCCGATGTCTTGGCCGATGAAGGCGAGCAAGTGGTTCTCGAGGTGCA GGCCAGTGGTGATCAACCCCTGACGGCACAGTGGTTCCTGACCAATAAGGAACTTCAACTGGATCAAAGGATTACCACAC AATCCGACTCCGAACTGGGAGTCTTTAAGCTCATCCTGAACAACGTGAGCGGCGATGACAAGGGAGTGTATACTGTTAAG GTGACCAATCCAGCGGGAGATGCCAAATGCTTTTCGCATCTCATAGTGAAGTCCGTGAATGCTCCCGAAAACCGTAGAAG TAGCCAATCATCGGTTGAAATCATTGAGCGTCACCAGTGTCCCGAATTCAAAGAACTGTTCAGTGACAAACAAGGTGAAA TCGATGAGGTGATCAAGTTCGAGTGCATAGTCAAGGGCAAGCCCACACCAAAGGTCCACTGGTTCTTCAACGATCAACCC GTCCATGGTCACAATTTCCTGGTCTCAACAAGTGGGGAACGTCAAGTGCTAACCATTCAGAAGCTAACCCACGATGCCGT TGGCAAGATCAGCTGTGTGGCTGAGAATGAGGCGGGCAAGGCCACCTGTGTGGCCTTTTTGAATATCCGAGGAAGTGGTT TGCCCGCCTCTAGTGATGTTCAGACCGTGAGTCAGGAGCACAACACCGAATCCTCGAGGGTCACGATCAAAAAGCAGACC TTTACCACCACTTCCACATCGCAGGTTAATTCCTATGAGGGAAATGCTCCTCAAACTGAGGTTCATCACTCCTCCGCCCA CATCGATCAGTCTCTGAAACAACTTGGACAACAACGACCGGAGATCGTGGAGAGCCATCACTACCAGGAGTTGCACAAGA GTAAGGAGATGAGCAGCCCCACAGTGCAGCAAAAGTCCTTTAGCTTTATTCAGTCCAGTGGAGCCAATGGACAATCCGCA GTAGCAATACCGGACTCCCCAACTCGCCTCAGAAGAGAAATAGCTCCCAGGTTCACCACTCCACTGAGTGGAAAAATCGT TGATCAGGGCGCCGATGTCAGCATGGAGGCCATTTACGATGGCTTTCCTTCGCCCGAAATCAAGGTGGAGAAGAACGGAG GTCAGCTGTTTGAGGATGCCCACACTAGGATCTCCAACAAGTGCAATCGCGTGACCATCGAACTCAAACAGGTGGGCGTG GGCGATGCGGGACGCTACGCGGTGACCGCCTCCAATACTGTGGGTCAATCCACCAGCACAGCGGACTTGGTCGTTAAAAA GACCATATTCCCGCCTGTTTTTGGCCGACGTCTGCAGGCTCAGGTCAGCAAGAAGGGCGAGAAGTTGACTATGGAGGTGG AGGTCACCGGGTTGCCCGAACCTACGGTCACCTGGCTGAAGGACGATAAGCCACTGAAGGATGCTGGAATTTCCGAGCAT CGCTTGCTAGCCCAAGGCAACTCCTACAGGCTGATCATCGAAAAGGCACAAACCACGGACTCCGGAAAGTACATGGTTCG CGCCACAAATGCGGGAGGTGAGGCCAAGAGCATTGCCGACTGTGCCATCCTCGAGCCTTCGCCGGAACGCTTGCAGGAGG TGGTCAAGACTATTGTCTATGAGACGGGTCCTGTGGCTCCGGCCAGCGAATTCAAAACCGAAGGCTACAAGTACACCTCG TCGAGTATGACCAGTAACTACTCCCACAATATGCAAAGCAGCAGCAGTTCCTCCCATCACGAGACCAAGTTCTCTTCAAC ATCAGCACCGCCACCAGTGGTTACCTATCCCAGTCCTATTCCTGCCCAGAGGAAGACCCCGGCGGCGGAGTTCAGTGACT ATAGCAGTGAGATTGACGAACGATTCCGTTCCGTATCCCGCGCCAATGAATCCGATGCAGAGATCAAGGGGTATCGCGTG GTCTTTCCACCCACGCCTACTCCTCGTACAAACATCGCCACCAATGGCCACAAATCGCCAGTAGTGGTAATAACTCCATC GCCAATGGAATTTGAACCTACTCCTCAGGACTATGCCAGGCCCAAATTTGAACCGATTAGCAAGGAGATACGCCATGAAA TCAAGACGGAGACTAGCTCCAAGCAATCCAAGTTTATGCAAAACCAACAGCAGAACCAGCCCGTGTTTAAACCAAAACCT GTGGCGGCCAAGTTTATAGCGGCAACCCAACAACAACAGCAGAAACAGCCACAGGCCACATCCCGTCCGACCATGTACTA CAATGCTGTTGCTGGAGCTCCGATGCACGTGGCCAAGGTGGCCACCGAAACGAAGAATGTGATGCAGATGCACGAGTCCA CTGAGAGTTCACAGCGCGTGGTGAATATGCAACAGACCAAGAGGATAATACACTTCGATAGTCCGCAGGAGCAGAGGGAT CAGCAGATCCTGGAACCCTTCCCCTACAGTCCCGCCACCAGTCGTACGCCTTCGAGGCAATCCCATCTGCCACCGCCAGC CACGCCCACTAAATTTGTGCCCGGAGAATTCCGGGAGAGTGATTACGAGAGCGAGATTGATGGAGCTCGCATCCAACCAT TGTGGTCTCCATACGGCGATGGTATGACTAAAGGATTCAGGCGAGTGGCACCACCTCAAGGAGCTGGAAGATCGTGCAGT CTGCCACGCACCTATGAAAGGGTCTTGTCCCCCATGGAATTCGATCGTGGTCCCGAGATGCCTAGCAAAATCCACGTGGA TATAAACACCCTAAGGAAAGAGCAACGCGGAGGCAGTACAGTGACCACCCAAAATCGTACTCAATCCTTGAACAGGAACA CCACCATGAGGCAACAGCAACAGCAACAGCAGCAGCAGTCCATGGATCAGATCGATAGGGCCGGAACTCTGCCGCGATAT GGTTATAGTTCCCTGCAACAGCAGGCAGAGAATCAGGGTCGTCGCATGGGCTCCACCTTTCTCCAGAAATCGCATCAGTT CGTCGACGATGTTAGCAGGGAGATCAGGAGCTCCGCTTCGAACGGCATCCGTCCGGGTTTCAAAAGGGCACCCAGCGAGG GCAGCAGCCAGCAACCGCAGGCCTTCCGGGATGAGTCGCGCGTCTCTCAATACGTGCCGGAGCTGCCCAATACGGAACCC GTCAATGCGCATCTGAGTCGCGATGAGCTGGCACAAAGGCAGCCATTATTCATAACGCCGCTGAAAGACATCGCGGTCGG AGTTGGAGGCACGGCGCGCTTCGAGTGCATTGTGCAGGCTCATCCGCAGCCGCAGGTCAACTGGACGCACAACGGCGGTC TTTTGGAGTCGGGTAGCAGGCACTGCATCGAGTACCGGAACGGCGTGTGCCGGCTAACCCTGCCGCAGGCCTATCCGGAT GACAACGGCAGCTACGCCTGCACCGCCATCAATCCTCTGGGAGCGGCCACCACCAGCGGAAATTTGACCGTTTCGAGCAC AAACCGCGGCTTTAGATACTAAACTTGTTTAAATGCAACTATTATTATTATTATTATTTGCTTTATGATTTGTTCAATGC CAAAGTTTCGCCTTATTGTGTGTATGTATTTATTGCGCCCCCGCGTCACTTATTCGTCTTGTGGGCTGATGATGGACTTA CAAAATGTCGCCCAAAACAACCCATTATACATGCATACAACAAAGGAATATAGTATTACTTGTGTTTTTGTCGAAAATGA AATAAACAAATATTTAAACGAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.