FB2026_03 , released September 17, 2026
Clone: Dmel\GH18129
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General Information
Symbol
Dmel\GH18129
Species
D. melanogaster
Name
GH18129
FlyBase ID
FBcl0133179
Feature type
cDNA_clone
Computed gene(s)
Dmel\IntS8
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI09622
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,298
    NCBI
    AY058406
    Full Length sequence dataSequence Downloader
    CCACAGCCAGCTGGTCTCCCTTCGCCATTCTTTAATTTTTGTTTTGATTGGTGAATTTTGGCAGGGATTAGCATGGACGA
TCCTCTTAAGCCCAAGCCAGTTCCACTGGCTGCCGAAACGGTACTGTGGTTCGAGTTCCTGCTCGATCCGCACAAGATTA
CGCAGCATCTGCAGCGCCCGCATCCCGAGCCCAGCGCCATGGAGCTGATCGTGCAGTTCATCAGCATGACGCCGAACACG
GCGCAGGAGTCAGTGGGGACGCCTGGCAGTGATTTGCAGAATCTAAACCAGACGCCATCGAATTCGGGACCCATTCCCGG
CGTGGTTGGTCGTGCCCCTGCGCCGACAACGCCCACTGCCTCCGGCGGAGTGGGCATGCCCCATAGCCCACAAAGGCCCG
CGGAGAAGGGCCTGCAATTGAACCGCAAGCAGTTGGCACTGAAAATCCTCGAGCTGAAGGTAGCCACTTGGCTGAAGTGG
GATCTGGATGCATTGGAGAAGAACCTGCCTGTGATTATGCAGCTGGCCTTGCTGCGCGATCTGTGCACCATAAGCTACGG
CTGCTCCTTAAGTATTCCCCTGCCCAATGATTTTGACGCGAGAATTTCCGCTGCTGGAAACGAAAGAGCAGCAAGATTCG
CCTTGACCATATACCACCGCATGCTGCTGCGAATGCAACTGATAAAGGAGCAGGCGTTAAAAGCGCCACGTCCTCAAAAC
ACAATGTACCAAACTGTGGACCAGCTCCAGCAGTTTCTGGACACACCGACTCAACCATCCATCGAGTATTTGCAACAACT
CTGCGCCTCGACAAAACCTTTCTACATATTTCACTACGACAGCTTTGTTCCGCTGCGATGTGATGATATCGGCAATGGCC
AAAACTACGATGTTATGCACCTAATAACACCCCAGGAATTGAGAGCCCAACTGCACTACGAGCTCGCCCAATATTACCTG
TACACCAAACAGTATGTTTTGGCCCGGGAAGCGGCCGCCGCGTGTAATACTAATCTGCAGGCAATCCCGCCACAGACAAC
TCTATATTACTGCCACATACGACCGTCAGAACTGGAAGGTCTACTGCAGGCCTGTGGAATTAGCGCCGAGGAGCAGTCAC
TGCTGGAGAAGTTCCAGCAATCGCTACTTAACAACTACACAGACATTGTATCAATTCTGCGAATGGACAACAGAAGGCGA
GAAATCCCCTTTATAAGCCGGCGGCAAGTGGAACTGGACATTGAGGGTTCCATCTCCACGGGCATACTCAAGGAGACAGT
TCAGCTGCAATTGCAGGTGGCTGCATTGAATGTGGTGCGAAACATCTTTGAGTGGGGCAGCATCTTTGGCAGCGTTGAGT
ACTTTGAGAAGTACCGGGAACTGGACTGCCTGCCACCGCTGGTGGAGGCTTTGCAGGAAATGCTTCCCCACTGTACTTTC
AAAGAACAGGCGGCACTCAAGCACTTCCTCATCGACTGTTTGCTTCATCAGGGTGGGCAGTCACGACAGTTGCTACAAAC
GGTGCGAGGATTTGGACTGTTCTCGTCAGATGAACTCCAGGATATTGACGAACAGATGCTGCAGGCGACGCCGCCGGTGC
CCACCAACTCGTTGGCCTCGCTTTCCGACTGGATGTGTCACTCCAAGATGAGCAGGGTTGACGTGGGCGCTCTGGAGCGA
CAACTGATAAGCTGCACCAATGCCAACACAGTGAGGATATTGCTAGTGAAATTATGCGCAACTGCTCCTGGAAAACCTCT
GTGGGCCATTAATCCCAGTTGGGACGTACCACAGCCCCTGAAAACTCTTATTATGGCCATGCCGGTTAGCTTTTTGCAAG
ACTTCAGCTATGTTCTGCTCGGCAAGGCGCGAGAATTGGCCACAAGGGGCAACTACATCGATGCCGTTTCAATGCTGAGT
GTACTGAAGTCGGAAAATCAGCGCCAGGAGATGGCGGCCAATGTGCAGCTGATGTGTAAGCTGATCACCTGGGAAATCCT
GCACATTCAGATAACACAGTGCCTGGAGGAGTGGCACCAGAAGCCACTCGATCTGCAATCCTTGGGAGGAAGATGCAAGC
AATGTTTGGGAGCCCTGCAGGCAGGAGATTCAATCGTCCCACGTCCGGATATCCTAGAGAGTTGCGCTATCATGCTACTT
AACCTCACAGAATTTCCACCTCTCCTTTACCTGGATAAACGGGCGGGTCCCCTGGAACTACCCCTGGCCTTCGCCGCCAC
CTTCATCGAGATGGAGAAGATGAAGGGCCCCAAGAAGGTATGTCGCGATGCCTGGGAACTGATGCTAAGCATGTTCCTTA
ACGTTCCGAAGCGTGGCTCCTCAGGAGTTGGCGGTATCAGTTCCCTGCAGGCCTTCCTACAGCGAATCCGCCACCAAAGC
GTTTTCGGACTCGCCATCTCCATGATAGGCAAAGTCCACAACATACTTAAGGATGACCCCAATCATGATTTAAGCTGCGA
GTACATGCAGCTGTGGCCTACCAGCATTAACAACCCTGTCAGCTACAGTTTGAGGAGCGTTTGCGAGACCCTGCAATGGC
TTCTTTCCGAGGCTTTGTCCTACTATCCGCAAACCATTTCATGGCTGAAGATGAAAGGCGATCTAGATCTAGCCATCGGC
AATAATGAGTCCGCAATGCGTTGCTATGTAAATGCGCTGGTTACTGGCACCGACTACTGCACCATGCCGCTGCAGAGAAA
TGTGGCCGACGACTACGTGATCCGCAAGATGATTCGATGTGCCGCCAATCTGGGCTGTCACATGCAGGCCACCGTTCTGT
GTCAGTTCCTTGATGAGATCGACTACGGCATTGTGTTCAAGAATCTCAGCGAGAAATCGTCGAATTTCACCGATGCCATG
GATGCATATTACAGCTGCATCTGGGATACAACTCTGCTGGAGTTCATCGTCAATCTGCACGCTAAGCGAGGCGAACACAG
TCGGAAACTTGAAGCGATTTCTATGATGGGCACTCTGGAGCTGAATGCAAACAACAATGAGGAGATCAAACGAGAATCTG
CCATGGTGCGGAAATCGAGATTTCTGCGTGCCCTGGCCAAGCAGTATTTGCTGTAGTCGCAGCCCCCACAGTCCAAACTC
TAATTCAAAATCCAATATCCATAATCCAACATCCCAACCTCACCCGCACCCACAAGCAGACCAACTAGCATTAAGCTCAA
AGATGTAGTTTATATTGTATGCATAGGCTTTACGGTTAAGTTAAGATTAAAGAATTGTACGTTTTTCCAGATAAAAAAAA
AAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)