FB2026_02 , released June 18, 2026
Clone: Dmel\GM30768
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General Information
Symbol
Dmel\GM30768
Species
D. melanogaster
Name
GM30768
FlyBase ID
FBcl0148468
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG10055
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI05809
Library
GM_pOT2_cDNA
  • GM cDNA library of D. melanogaster, Oregon-R strain, adult female, ovary, pOT2 vector.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    oogenesis stage S1 -- S6 & adult stage | female
    ovary
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,423
    NCBI
    AY118875
    Full Length sequence dataSequence Downloader
    TCATACTGGTCATTAAAATAAATAAAATTATACTAAAAAACAGTAGTTAGCACAATCAACAGTCTCGTAATAAAATTGTA
AGATGTTGAATGGACAGATTGCACACAGTTATTTGGAGAACTCGTGCACGCTGATCATACCCCGCCCGCCTGTCCTGAAT
CAGTCCTCAAAGTCGGCCCAGCCAGCAGCAGCAGCTGCTGCTTCTTCTTCCGCTGGTGAGGAGTTTGGTCAGGAAGCGGG
GTCGGGGTTGGCGGAAGCGGAGGCGTTGCATGTGGATCGCATAAGAAACTGCGATATATTCGTTGACGCGCACGATCTTT
ATTTCTACGCAGATGTTAAGCTGTACAAATTCCACAGCCGTCGCACCTTCGACTTGCGAGAGCTGAAGACGCTGTTGATA
AAGTTCAACACGACAGAGAATCACTACCAAATTTGCCTGCGCTGTCTGCCCCTAACAGCGGGTGCCCAGGAAGGACCCGG
TGGAGAAAGAGGCGGAGGCGGTGGTACGGGGGATGCAGCCAAGCAGTGGAATAGTCGCAAAGAGTACATAGCCGCATTCC
TGCACCTACCCACACTGAGTGCCAATTTGCCGGACAAGCAGCCTCTGGTGCAGGAATGGAAACTCAAACGAATCACCGAT
CAGTGCCAATTGCAGCTCGACGACCACGAGCGCACCTACAAGCTGACCAACAATTTTGGGTACGGCTATGCTAGCGAATA
CAGCGGACCGCTAGACCTGAGTGAGTTGGACAGAGCCACGTGCCGCGTGTCGGAGCCACATCGCATGAGCCCCATCCAAA
GGCGGCAAGAGCGCGTGGTCGATGAGATGAAGCGCTTTTCCCGCGAGCAGTACAAAATAAATTTTGTGGAGCTACAGGTT
CCGTCCGGTCACCAGAATCCTTTACGCTACAAACCGAAAATCTACGAGACTTCCATGACCCAGGAACAAGCGCATCTGCT
GCACAACATCAGCCGAATGCAGACACGGGAGCAGCAGCCGTGCGACGTTTTTCGGCAAAACGAGATCGATTGCGGGCTGA
TAAGCATTCTGTTGGCCATATGCTATGATGTGCGGACGACGAACAATTAGCCGACGTGTGAATCGGGCTGGACGCGAAGC
ATACTGTGCCCGCTTTATTGCTACTTTGAGCAATTCGACAACTATCGGGACGTACTGGTTGCCTTCCTGCGGCGAATGAT
CACCTACCCGCTGTACCGCAATTTCGAGTTGGGCCGCCAATGTGTACGGGATGCCATAGAAGTTTTAAAGGGCGGTCGCA
ACTGGTTAATTAATCAGCTGCTGCTAACGCACCAGCAGTTCGCCAGCAGTGATCCCAGTCGCGATAGCTTCAACAACTAC
TATTTGGAGGACTATATACGATATGTGACCAGCCCGAGCGCCTGCAGTGATGAGCACATGCGCCTTCTTGCCCGCAACCT
TAAGAACGTGCTGATGGACGTAAACAAGCAACACTTGGGCCTGGGAGTGACCGAGATTGAGACTGAGCTGATCAAGGAGC
TGATGCAGGAGATGCGCATCGATGCGGGAAGCCAGGGTTCCTCGCCGCAGCAGCAGCATGGCGATGATGACTTGGACCTT
GATAACGATACTTCTGGACAGGAGGACGAGACTACCACTGATGATGAAAGCGTCATAACTGACTCCTTCTACAGCGTGGA
CATGGACATGCGACTGATAGAGAACGAAATTGTATATGAGGATGACGAGGAGGACGAAGATGACGATGAAGATGGAGATG
ATGATGAAGATGGGGATGGGGATGAGGATGAGGATGAGGATGAAGACGAAGACGACAGCAGTTCTTCCACAACCACCAGT
AGCGAGGCGGAGGGAAACAGCGTCATCGAGCAGTGCAGCAACAGTGAGACAGCAGCCAGCACCACATAAATTGCCACTTA
TGTCGAGTTCGGCAAGTCCTGCTCCTGCCTTGCCCAAAACAAACAAGCAACACAACCAACTTAATGTCAAAGAACGGAGG
CTAAACAGTGATTACTTTTAAAATTAAAACAAAGTAAAATACGAAACTAAAAACCAAGCCAAGTAAATGATGTAAGTTTA
CACAAGAATACCCAAAAAAGAAGAACTTCTTATATCTTAATTGTACAATAATTTAACGAAAAGCAAACTCAAAGATTTGT
TCACGCCAAATGCGAAAAGATATTTGAATATTTTTTCCCCTTTGCTCTACAATTACTTCGATTATAATTACACATACAAG
TAAATCAGAACTTTAGCATTTAGTATGGCATTAGAGTATGAAACATTTGAATTTTGAATTCTGAAAAGCGAAATCTAGTT
GTGCATTTGTATATACATCTATCGATATTGTTGGTGCTGCAAGCAAAGAAAACAAAATATGAATAAAATAAAATGGAAAT
GGAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: GM_pOT2_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.

    (Rubin et al., 2000)
    Sample preparation

    Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.

    Protocol

    RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM12101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

    (Rubin et al., 2000)
    Comment
    Reported As
    Symbol Synonyms
    GM
    (Rubin et al., 2000)
    GM_pOT2
    (Rubin et al., 2000)
    GM_pOT2_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)