FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\HL04344
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General Information
Symbol
Dmel\HL04344
Species
D. melanogaster
Name
HL04344
FlyBase ID
FBcl0148793
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG9132
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pBS_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pBS vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,953
      NCBI
      AY121637
      Full Length sequence dataSequence Downloader
      ACTGGCTAAAGCTTAAAAAAACACTTGGAAAGATTCAGATAATAAACAAAATCAAATTTATCTGAACTTCGAATAAGATG
GAATACGAAAGTGTGCTGATCGTGAAGCCGGAGGTGTTTATCTACAAAATACCACCCCGTGCGAGCAATCGTGGTTATAG
AGCGGGAGATTGGAACTTAAAGGAGCCCACCTGGACGGGCAGGATGCGACTGGTGGCCAAGGGCACCGCCGTTGTCCTCA
AGCTGGAGGACAAGACCAGTGGTGCCCTGTTCGCCAACTGCCCCATCGACACATATCCCGGCGTGGCCATCGAGGCGGTT
TCAGATAGCTCCCGCTATTTTGTCATCCGCGTGCAGGACGATAATGGGCGATCGGCATTTCTGGGCCTCGGATTCGGCGA
TCGATCCGATTCGTTCGATCTGAATGTTGCGCTGCAGGATCACTTCAAGTGGGTAAAGAACCAGGAGCAGATCGAGAAGG
AGAAGACGGAGCCCAAGCAGGAGCTCGATCTGGGATTCAAGGAGGGCGAGACCATCAAGATCAACATGCGGATAACGAAA
AAGGACGGCTCCGAGGGATCCTCGCGCACTGGCAAGAACAAAGGCTCCTCGGGCGTGCTGCCACCGCCGCCCGGTGGCCT
TGGCAAGATCGCTCCGCCACCGGCCGCAGCCGCCGCAAACACGACGGTACGGCAAAGTCCCGGCGTTTCGCCCGCCCACA
GACCCGCCGCTGGAGGATCCGAATGGACCGACTACGCATCAGCCGGTGGCAACCAAGGACAACAGAACTCGGCCAACGCC
AACTGGGTGCAGTTCTAGATATACATCATCCTGTTCCTCCTGCTGCTGCTGCTGCTGCGCTCCTATCACCCAGAACTTTA
TGTTTTAAGACTCCAGATGTTTTTTTTTTTATTCACCATTTGCAAGCAAATTATTATCGTTTTGAACAAGACCAAAAGAT
TGAACATAACATTCAGCAAAGGCGTCACGCGTTAAAGTTAAAGATTTATAATTCAATTGAAATTGTGTATTAGTTTCCCC
GCCCACCACTCTCCCCCTCTCCCTCACTCTCTCTCTCTCTCTGTCTGCCTGCGTAGTTGCATCTCGCTCTAATTGAAGGA
GTACGATGAGCGTGGGCAGAGTTCTCAGTGGGCGGGGATCTGTAGCGAACTATTTAGCCTGGTTGTTAATTGTATAAGTC
AAAGTAACGATAATTTATATGGAAATTATACTAAAATGTAATTTGTTAACGTCGAGATAATGCAATTATAAAAATGCAAA
CTAAGTTTAAATCAATTTACACACGAACACAAACACTGCAACAGCTAAACTAAAGTGAAATATTCAACTTTTGAGTACTT
ACACACGCACGTAAAACAAGGACATTCCGCAGATGAGCGATAGGACGCACTACATGCCAATCTTATTTATTGTTAACTCT
ATTCCAAATTACCATCCCCTCCCCAACGATCCTATTGTTTCACATCCCCTACCTTGTATGGGAATACCAGAAATAATGCA
GATCTATGCACCTGTTTCCCATTTTTTAACTCGACTCACATTGTTTGAAATCGGAATTGAATTAAACGATCAGTTTTATG
GTTTCACCTTATTTGAGAGGTTCGCAATCGGGATCAGGTCATTATATATACATATATATATATATATATATATAGTTCTG
TTTTCAAAAGTGGCACACACACATGCATAGCGTTTAAGTAAATCAAATGAATTATATATACATAATATACAATATGAAGG
AATATCCATATGTATTTAATAAACCACATAATTGTAGAGAAGGAAATCTTGCTAAATCTGAATTGTTGTTTCAGCCCCGA
TTACCCCGCTCCCTTCACCTCCGCGCCACCACAAATCAATATCCTATAACTATGCTCTCTCGTTTCTGTTGCTGTTAAAA
TGATAACATCAAAACAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: HL_pBS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      HL_pBS
      (Rubin et al., 2000)
      HL_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)