FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\HL01061
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General Information
Symbol
Dmel\HL01061
Species
D. melanogaster
Name
HL01061
FlyBase ID
FBcl0149707
Feature type
cDNA_clone
Computed gene(s)
Dmel\Elk
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pBS_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pBS vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,764
      NCBI
      BT099612
      Full Length sequence dataSequence Downloader
      TTGAGTGGCTTCGAAAGTCGGACGTGGCGAAAGTTCTGTTGAATTGATTTGAGAACAGCATAAAACAAGCAGCAACAGGC
AACCAGCAAACAATCATCGGCAAATCGACAAGGAGGCTGGAAAAAATCACCCGCTCCTCCAGAAAAAGCTTTGCCACGAC
CCAAGCCGCGCTTTAAGCGGCGCACCGGCCCCACCATTCCCATGCCAGCCCGCAAGGGGCTGCTAGCGCCCCAGAACACA
TTCCTGGACACCATCGCCACCCGGTTCGATGGCACCCACTCGAACTTTGTGCTCGGCAATGCGCAGGCAAATGGCAATCC
AATTGTTTATTGCTCGGATGGGTTCGTGGATTTGACAGGATATTCACGCGCGCAAATTATGCAAAAGGGCTGCTCATGTC
ATTTCCTTTACGGACCGGATACGAAGGAGGAGCACAAGCAGCAAATCGAGAAAAGTCTCTCCAATAAGATGGAACTGAAG
CTGGAGGTTATTTTCTACAAGAAGGAAGGTGCCCCCTTCTGGTGTTTGTTCGACATTGTGCCCATCAAGAATGAGAAGCG
CGATGTGGTGCTCTTTTTGGCATCCCACAAGGACATCACGCACACCAAGATGCTGGAGATGAATGTGAACGAGGAATGTG
ATAGCGTTTTTGCCCTTACAGCTGCCCTTTTGGGCGCCCGGTTCCGGGCCGGCTCCAATGCCGGAATGCTCGGCCTGGGC
GGACTGCCCGGTCTGGGCGGACCGGCTGCCAGTGACGGGGATACGGAGGCGGGTGAGGGCAACAATCTGGACGTTCCCGC
CGGCTGCAACATGGGTCGACGGCGCAGTCGAGCGGTTCTCTACCAGCTATCCGGGCACTACAAGCCGGAAAAGGGTGGCG
TGAAGACCAAGTTGAAACTGGGCAATAATTTTATGCATTCAACGGAGGCGCCGTTTCCGGAGTACAAAACCCAGTCGATA
AAAAAGTCACGCTTGATTCTGCCCCATTACGGGGTGTTCAAGGGCATCTGGGATTGGGTGATTCTGGTGGCCACCTTCTA
CGTTGCCCTCATGGTGCCCTACAATGCCGCGTTTGCCAAAGCGGATCGGCAGACAAAGGTATCGGATGTGATTGTGGAGG
CGCTCTTCATTGTAGATATTCTGCTAAACTTCCGCACCACCTTCGTGTCGCGCAAGGGCGAGGTTGTCTCCAATTCGAAG
CAGATTGCCATCAATTACCTGCGAGGATGGTTCGCCCTGGACCTTCTGGCCGCGCTGCCCTTTGATCACCTGTACGCGTC
CGATTTGTACGACGGCGAGGATTCGCACATCCATCTTGTGAAATTGACGCGTCTCCTGCGGCTCGCTCGTCTGTTGCAAA
AAATTGATCGATACTCGCAGCATACGGCCATGATACTGACATTGCTGATGTTCTCCTTCACGCTGGCCGCCCACTGGCTG
GCCTGCATCTGGTATGTGATAGCGGTCAAGGAGTACGAATGGTTCCCCGAGAGCAACATCGGTTGGCTGCAGCTTTTGGC
GGAAAGGAAAAACGCTTCCGTCGCCATTTTGACCACGGCGGAAACGTATTCCACGGCCTTGTACTTCACCTTCACAAGTC
TCACTTCCGTGGGATTTGGCAACGTTTCGGCGAACACGACGGCCGAAAAGGTCTTCACCATCATCATGATGCTCATTGGC
GCTCTGATGCATGCCGTGGTCTTCGGTAACGTGACTGCCATCATCCAAAGGATGTATTCGCGTCGCTCGCTATATGAGAG
CAAATGGCGCGACCTAAAGGACTTTGTGGCCCTGCACAATATGCCCAAAGAGCTAAAGCAGCGCATCGAGGACTACTTTC
AGACCAGCTGGTCACTCAGCCATGGCATCGATATATACGAGACGCTACGCGAATTTCCCGAAGAGCTCCGTGGCGACGTT
TCCATGCATCTACATCGTGAAATATTACAGCTGCCGATATTCGAGGCGGCCTCCCAGGGCTGCCTGAAACTTTTGTCCCT
GCACATTAAGACGAACTTCTGCGCGCCCGGGGAGTATCTGATCCACAAAGGCGATGCCCTCAACTATATCTACTATTTGT
GCAACGGATCTATGGAGGTGATCAAGGACGACATGGTGGTAGCTATTCTGGGTAAGGGTGACCTGGTGGGCTCCGACATC
AATGTGCACCTGGTGGCCACCAGCAATGGCCAGATGACCGCCACCACCAACAGTGCTGGCCAGGATGTTGTCGTCCGCAG
CAGCAGCGATATTAAGGCGCTAACCTACTGTGATCTGAAGTGCATCCACATGGGTGGTCTGGTGGAGGTACTACGCTTAT
ATCCGGAGTATCAGCAGCAGTTTGCCAACGATATTCAGCACGATCTCACATGCAATTTAAGGGAGGGCTACGAGAATCAG
GACTCCGATATTGGACCCTCATTTCCCCTGCCCAGTATCAGTGAGGATGATGAAAATCGCGAGGAGGCCGAGGAAGGGGG
CAAGGGGGAGAAGGAAAACGGTGGAGGACCACCAAGTGGTGCTTCTCCGCTGCACAACATAAGCAATTCTCCGCTGCATG
CAACACGATCTCCACTACTGGGCATGGGAAGTCCCAGAAACCAAAGGTTGCATCAGCGGGGTAGATCTCTGATTACCTTA
AGGGAAACGAACAAGAGGCATAGAACTCTAAACGCAGCTTGTAGTCTGGATCGTGGTTCCTTTGAGGAGCCCGAACCACT
GGAAGAGGAGCAATCGTCGGGCGGTAAAAGGCCCTCCTTGGAACGCTTGGACTCCCAAGTGTCCACTTTGCACCAGGATG
TGGCCCAACTGAGTGCGGAAGTTCGTAATGCCATCAGCGCCCTGCAGGAAATGACATTCACCTCCAATGCCATGACATCA
CATAGTTCTCTCAAGTTCCCTCCAGCAAGATCTATACCCAACATAAGTGGAGTTGCGGGCACAAGATCAGGAGTTGCAGT
CGAGCACGGCTTGATGGGCGGAGTTTTGGCAGCCGCTGAATTGGCAGCCATGCAACGTAGTTCATCACATCCTCCCGAGG
TTTGGGGCAGGGATGTCCAACTGCCCACCAGTAATACGGCCAGTTCCAAGGCACCCTCACCTGTGGAGCCAAAGAAGACG
ATGACCAGTAGGAGCAGCCAAACGGACTTCTATCGCATAGACTTTCCGACCTTTGAGCGATTCGTTTTGGCCAATCCTCG
TCTGGTTTTGGGACTTCTGGGCATTGAACCCGCCATCAAAAACGAAATGGATCTCCTGCAGCAAAAGCAAACCCTGCAGA
TATCACCTTTAAATACGATAGATGAGTGTGTTTCGCCTTCGGATCACAATTTGGCCAGTTCCAAGGAGAGACTAATCACA
TCATCGGCAGTCCCGACACCTGGTCGCATTTACCCGCCTCTGGATGATGAGAACTCCAATGATTTTCGGTGGACAATGAA
GCACTCGGCCAGTCATCATAGCTGTTGCAAGAGCACTGATGCTCTGCTGAGTCCAGAGGAGCAGCCTCCGATTTCGATAC
TTCCAGTGGATGCAACTCCTGCGCCATCTGTCCAGGAAGTGAGATCCTCGAAAAGAAGCTTACGAAAAAGCACGAGTGGC
AGCAACTCCAGCCTATCAAGCAGCAGTTCGAGCTCTAATAGTTGTCTGGTGTCGCAGAGTACGGGCAACCTGACCACTAC
GAACGCCTCGGTGCACTGCAGCAACAGTAGCCAGAGTGTGGCCAGTGTGGCGACCACGAGAAGAGCCTCCTGGAAGTTGC
AACA
      Library Information (1)
      Library: HL_pBS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      HL_pBS
      (Rubin et al., 2000)
      HL_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)