FB2026_03 , released September 17, 2026
Clone: Dmel\HL01444
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General Information
Symbol
Dmel\HL01444
Species
D. melanogaster
Name
HL01444
FlyBase ID
FBcl0149905
Feature type
cDNA_clone
Computed gene(s)
Dmel\NimB2
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pBS_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pBS vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,451
      NCBI
      BT012496
      Full Length sequence dataSequence Downloader
      GCAACTACAGTCGCCAGCGGACGTGGACGTGGGCGCATAATCAATTTCCATACGTACGAAATCCGTAATCAAGTGACATC
ACCATGAGGTCATCATCGTGCCAGCTGGTGACTCTGGGCGTTCTGCTGGCCATCTGTTCCCTGGGTCAGGGACAATTCAA
GACGGCTGGCATCAAGACACGGCAGCCGCCATCGGGAAACCTGCAGCTTGCCGGCAATAGCAGTGAGTCGGGATGGAGGA
GCTACAACCAGAGCAGCTACGGTTGGAGCACTCAAAACCAGAGCAACTACGCCTGGAACCAGCAGAACCATGTGGAACAG
GGATCCGCTGGATTTGTGCGCGCCGAGGTCTTTCAACCGGTCACATTGCCGCCCCTTTATGGACACTACGTGCAGCCGGT
GACTCCGCCCGCTCATCGTGTCCAGGTCCTGGATGAAACGGCCCTGTTCATCAACAAAACCAGATCTGCCATGGCCAGTG
GTGTGTGCTACAAGGAAGTTCCAACCGCGTCACTGCTCCGCAATTCGCGGGATCAGTTCGTTGGCAATGGCACCACTCCG
GACATGAGTCGCATCCAAGTCTGCTGCGATGGCTATGAGCGAAATCCGCACATCTACCGCCGCTGTGAGCCGATCTGCGC
GGATGACTGCCGGAATGGAATATGCACTGCTCCGAATACCTGTGTCTGCATACCCGGCCACGTTCGCACCGCCGAGGGCA
AGTGCATCTCCACCTGTCCCCTGGGCTGCGGCAACGGAGTGTGCGACGAGCGGAATGAGTGCAAGTGCCGCGAGGGATAC
TCGCTTGAGCCGGAGACTCGTAAGTACTGCCAGCCGGAATGCAAGCCCGGCTGCTCCTTTGGACGCTGTGTGGCGCCCAA
CAAGTGCGCCTGCCTCGACGGATACCGCCTGGCCGCCGATGGATCCTGCGAACCAGTGTGCGACAGTTGCGAGAATGGCA
AGTGCACGGCTCCAGGACACTGCAACTGCAACGCAGGCTACCTAAAGCTCCAAGGACGCTGCGAACCCATTTGCTCCATA
CCCTGCAAGAACGGACGCTGCATTGGACCGGACATCTGTGAGTGCGCTTCCGGTTTCGAGTGGGACCGGAAGTCGGCCGA
GTGTCTGCCGAAGTGTGACCTTCCGTGCTTGAACGGCGTCTGTGTGGGCAACAACCAGTGCGACTGCAAGACCGGATATG
TGAGGGACGAGCACCAGCGGAACATCTGCCAGCCACACTGCCCGCAGGGATGCCAGAACGGTTACTGCAGTGCCCCAAAC
TTCTGCATCTGCAGGCCGGGATTCATCAAGAGCGGCATCAAGGGACGCCAGACCTGCCAGGCCGTCTAACTTAGACTTTC
GCACTTTATTGTTTCGTCTTGGATCAGAAATTAAATAGACGCCCTATCAGGGCATTAAACTCATAAAAAAAAAAAAAAAA
AAAAAAAAAAA
      Library Information (1)
      Library: HL_pBS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      HL_pBS
      (Rubin et al., 2000)
      HL_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)