FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\HL01032
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General Information
Symbol
Dmel\HL01032
Species
D. melanogaster
Name
HL01032
FlyBase ID
FBcl0150564
Feature type
cDNA_clone
Computed gene(s)
Dmel\MsR1
 
Collection Status
BDGP
Known Problems
chimeric clone
FlyBase assessment
  • Caution: This cDNA clone replaced by FI12701
Library
HL_pBS_cDNA
  • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pBS vector.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,274
    NCBI
    BT053703
    Full Length sequence dataSequence Downloader
    CTGGGAAGGCGGCACTGCACGGCTGTCCTTGGCAGCAAATCCAGTTGCAACATCGGTGAGTGACGGGCGGCAATCACAAA
GGACTTGCAACAGTTGCCGTTGCTTCTGCTATTGCTGTTGCTGTTGCTGTTGCAACAATGGCCAGTGGCAACAATGAAAC
TGAGCCGCTCTACTGCGGCAGCGGCATGGATAATTTTCATACAAGCTACAAGAACATGCATGGCTATGTTTCGCTGGTGG
TCTGCATCCTGGGCACCATCGCGAATACCTTGAATATCATTGTGCTAACCCGACGGGAGATGCGCTCCCCCACGAATGCC
ATACTCACGGGTCTGGCCGTGGCCGACCTGGCAGTTATGCTGGAGTATATACCCTACACCATACACGACTACATCCTGAC
GGACAGTTTGCCGCGGGAGGAGAAGCTCAGCTACAGCTGGGCCTGCTTCATCAAGTTCCATTCGATTTTCGCCCAGGTTC
TGCACACCATTTCCATTTGGCTGACGGTGACCCTGGCTGTTTGGCGTTATATAGCGGTGGGTTATCCGCAAAAGAATCGC
GTATGGTGCGGTATGAGAACCACCATAATAACGATAACCACCGCTTATGTGGTGTGTGTTCTGGTGGTGTCGCCGTCGCT
CTATTTGATCACGGCTATAACCGAATATGTCGATCAGTTGGATATGAATGGCAAAGTGATAAACTCCATTCCCATGACCC
AGTACGTAATCGATTATCGTAATGAGTTACTGAGTGCCAGGACGGCTGCCCTGAATGCCACGCCCACCAGTGCACCACTG
AACGAAACTGTGTGGTTAAATGCGAGCACCTTGCTGACATCGACAACCACCGCTGCACCACCCACGCCATCGCCAGTGGT
GCGAAATGTTACTGTCTATAGGCTATACCACAGCGATTTGGCGTTGCACAATGCCTCGCTGCAAAATGCCACATTTCTCA
TATACAGTGTAGTGATTAAGCTGATACCATGCATAGCACTCACCATTCTGTCGGTTCGATTGATCCTGGCCTTACTGGAG
GCCAAGCGGCGGCGGAAGAAGCTCACCAGCAAGCCCGCCACTCCGGGTGCCAGTAATGGAACCAAATCACCGGCCAATGG
TAAAGCAGCGGATAGCCCCCGGAAAAATAGCAAAACTCTGGAAAAGGAAAAGCAGACGGATCGCACCACGAGAATGCTGC
TGGCGGTGCTACTCCTCTTTCTCATCACTGAATTTCCACAAGGGATTATGGGTCTGCTGAATGCTGTGCTCGGAGATGTC
TTCTATCTGCAGTGCTACCTAAGACTGAGTGACCTGATGGATATCTTGGCCCTGATCAACTCCAGCATCAACTTCATTTT
GTACTGCTCCATGAGCAAGCAATTCCGCACCACGTTCACGCTGCTCTTTCGTCCAAAATTCCTGGACAAGTGGCTGCCGG
TGGCGCAGGACGAAATGGCAGCTGCTCGAGCTGAACGCTCTGCGGTGGCACCGGTCCTGGAAAAGGGACGACAGCAGCCG
CAGGTGGTGATGGCCAGCACGACCACCAACATCACGCAGGTGACAAATCTGTAGCACAGGAGGAGTCGTGGTCGCCGAAC
TTTGCTCAGTCGCCTGCTGAGTGTCCTGAAACGCGGCAGAAGGCGCTCCTCCGGCGAAGGAGGAGGCGTGGGAGGAGGTG
GTGCGCCGTTGGCCGGCAACGATGCGGTGGAACCAGCGTTCCAGGCCATCGTGGTGGTGGTGGACAAGGTGAGCGGTGCC
ACGGAGAATCAGCTGTACACCGCCGAGCAAGCTCGTATTGTGACGTAGTAACCCTGATAGTAGTACATATTATATGCCTA
GTTATCCTTGTAAAAAGTAAACTAGTTGTGTAAGAATCGCAGATAACCACAAGTTATGTGCATACTTTGGTGGGAAATCG
TAAAACCCGTACAGTCATAATAATAAAATGCTGGTTCATCACTCATACGCACCGTTTGACTTCACTCTATTCCACATTCA
CAGTCAAGTAGGAGCTGTAAAAATGCTGTGCATAAACGAGCTCGTATACGCTACAGCTGGCGTCACAATAATAGTTACCA
TTGGGTTGGCTTCACAACATAGATCAAAGTCAAAAAATATATAGTTTTGTAAAATGTTTCATAAATCTATCTTCATATTT
AACTAACACAATTCAGAAACTTTCGCTTGCATGGAAAATGTTTTATTTTCCCCACTTGACCCAATTGTGCGCAACTGTTC
AGCTTTTCGGCATGAATCAAATCAAATATAGTAAACTTTTGCATCTTTTCCACTTTGATCCCATGCGTTTTCATGTTGAT
TTCATTCGCATAAAGTATGCAGCACACGTACTCACTCAAAACTACGAATTTTCGGCAACGATTTGAGCAGAATATTTGCC
ATTTGTTGTACAGATTATATATACACACACACACACTTATGTCCGTATCTTTAATAATGGCAATTAAGTCCTAGAAATTT
TGACCTGATATTGATGCAGGCATTGGAAAAATAAGTGGCAGTTGCAGTGCGGTGCGTTGATAAATGAAATACTTTGCGAA
TTCTATTATTGGAAATCGCAATTTGGGCTGTGAATGAAATTATAGTTTTCTTGAACGCAATTGAGAATTCAGTTCCGTTT
CGTGGGTTACTGAATTTATGGGAAATAATTTAAGGCAGTCACTGCAGCTGACCGAATAATCCAATGAAACATTGCATAAA
CAGTTTGCAGTTTTTCCACACTTGCGAAAATTGTAATTGCAAATCGCTTAAGCCCCACGACGAATGACAAATGGCCAGCG
AACGCATAAATTATGGTGATTGAAGTATTTGCACTTGAGCCCCGACAAATATTTTCAATCTCGTTAATAATGAATACATA
AATCAAATTACGAATAGGTACATGTGAATACACCAAATATATTGTATGTAACTGTATGTTGCTTGAACAACGATTAAATT
AGCTGCTAAATTAAAAGAAATTATATTAAATTAAATGAATTAAATTATAAAGTTGCCACATTGCTTACTGAAAGTAAGCA
TAAATAATAGATTAATTGTTGTTTGTTATGCCTAATTAGATTGACGGATAAGTGTGATTTAAGCTACTCAATTAACCAAT
CGAGAGCCATCAAAGGGTTTATTAGAGCGTAATTAAGTTTTATATAACTAAAAAGATTTAAATCGAATTGAAAAGCTCAC
TGATTGGTATTTAAATTAAGCAACCGCTAAACATAATAAAATTTAATTGAACACACAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: HL_pBS_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    HL_pBS
    (Rubin et al., 2000)
    HL_pBS_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)