FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\HL03687
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General Information
Symbol
Dmel\HL03687
Species
D. melanogaster
Name
HL03687
FlyBase ID
FBcl0150809
Feature type
cDNA_clone
Computed gene(s)
Dmel\B52
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pBS_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pBS vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      2,295
      NCBI
      AY069302
      Full Length sequence dataSequence Downloader
      CTACGACCGACCTCCACGAAGCGCCATCGCCGCCGCCTCATCCATTCGTCGTCTATTCCAAATAAAACATAATATAGAAC
ACATAACGAAATGAACAAGTCGATCAGAATCGAACCCAAAACTGAATGCAAAATCGAAATCAGAAAGCAGACGATGACGA
TGGACGTTGATGAGCTGCCCGGGTCAAAAGGCCAGCCCACGTATTAAAGCCCACACTGATTGAAAAGACGTGGGCTGGCC
TCTTGCGTTAGGTATTTAACTCTCTGCTAAAAACATTCACAATTTTAATTACCAACAATATTATATAAAGAATTGTCAAA
TTCCTTATAGCCATTGTGAGTGTGATGAGGGTGCGAATCGAATCAATCGAGTGTGGTTAGATGAGAAGCTTGCGTTCGCT
AATTCTAAGATGATGTGATGGTCTTGGCGACGATGAGGGATGAGCATATGACGATGATCGATCGATGACGATGACACGAT
GCTGCGATGCTGGTCGCGCGTTCAGTTACCAACTTCGGCTAGTGCCTCCGAACTTATTCCTTCCCCCCATTCCCGGTTCT
TCTGGCTTCCGGCTGCGGCTCTGGCTTATGGCTGCGGCTCCGGCAAACGGCATACGGCTACCTGCCCCGTCTCCGGCTGA
GACTACCGACTAGACAACCACCAACCCAACCCGTTGGAGAAGCGAGAGCAGCGTCCCGAAACGTATTTCTTATTTTTGCA
GTTCGTTTGTGCGTGAGGTGCCGATGCCGCTGGTTGTTGTCCGCCTCTGACCGGCAAACTGCTCCAAATGCTCCATTCTC
TGGCTCTCTGGTGCGGTGGTTCTGGCCAGCGATGTCTGCTCTGATCTGATCTGACCTCCTCCTGACTGTATCCGGTGTGT
GGCTTCCTCCGAAACGAAACGAAAACAGCTACCGCAACACTTGAATACCTTTTTGCCATTTATAATATGATTCTGTTGTA
CATGAAAAACATAACAAAACTGTGTACTTGGTGGTACGTACGTGGCCAAATGAAAACGAATACCAAATCCAAACAAACAA
AACGTAACGAGCTCTGGCCATCCATCCGCTCATCCGCCTGCCTGGCCACTGGTTCTCTTCTGGCTGACTGGTTGAGTTAA
CGATGTTCGATCGGACTTTCGATGAGGTCATGAGGTGTCCTTGGTGTCATTGGCCCACAGTTACTAGGCAGACAATCTAA
ACTCTCGGTGTAGAAACGATGTGTGTGTCCTCCTCTCTCTGAAAGCGCATTAAGATCTCAAGGATTACATGCGCCAGGCT
GGCGAGGTCACCTATGCCGATGCCCACAAGCAGCGTCGCAATGAGGGCGTGGTTGAGTTCGCCTCGTTGTCGGACATGAA
GACGGCCATTGAGAAGTTGGATGACACCGAGCTAAACGGCAGGCGGATCCACTTGGTCGAGGATCGTCGCGGAGGACGCA
GCGGTGGTGGCGGCGGCAGCGGACGTGGACGCTCCCGTTCGTCCAGCTCCCGATCGCGCTCCCGATCCCGCAGGCGTTCT
CGCTCTCGCCGCTCGTCGCACTCGCGCTCCAAGTCTCGCAGCCGCTCCAAATCTCGTGGCGGACGCTCCAAGTCCAAGTC
GCCAGTCAAGTCTCGTTCTCGCTCCCGCTCGCGATCCAACAAATCGCGTGACGTGTCCAAGTCCAAGTCAAAGTCCCACT
CCCGCACCCGCTCCCGTTCGCCCAAACGTGAGCGTGACTCCCGTTCCCGATCGCGGTCCGTCTCGAAGCGCGAGTCCCGT
TCCCGCTCACGTTCCAAGTCCATCCACCGCGACTCCCGTTCACGCGATCGATCCGCATCGGCTGAAAACAAGTCGCGTTC
GCGCTCACGCTCCCGTTCGGCCTCGCCCAAAAATGGAAACGCCTCACCGGACCGCAATAACGAGAGCATGGACGATTAGA
TTAGTTAGCTGTCCCATTAATATTAAAACACTTGATTTTAGACTTCTACTATAACTATTCTACAAACATCAACACTCAGA
CACCCCGACTACAACCAAAACTTAATGAATATGAAATCATACCAATCCACCACTGTCGGGCCTCGTCATTTTCTTTTAAA
TATATAACGACAATTTGTCCTAAGTCTTGTTGTGGACATGGGACATTGGCTGGAAGATGACAGGGATCGGCTGAATGTGC
CGATTTGGATTCAAGGAGTACATATAGGCCACGAAATTACTGCGACTTGTACACCGGAACACTTAAAATTGTCTATACAA
TGAGTAATTTCAATAAATACGGATAAATTTAAACTATAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: HL_pBS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      HL_pBS
      (Rubin et al., 2000)
      HL_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)