FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\HL07802
Open Close
General Information
Symbol
Dmel\HL07802
Species
D. melanogaster
Name
HL07802
FlyBase ID
FBcl0150857
Feature type
cDNA_clone
Computed gene(s)
Dmel\5-HT2A
 
Collection Status
BDGP
Known Problems
incomplete CDS
FlyBase assessment
    Library
    HL_pOT2_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,485
      NCBI
      AY061039
      Full Length sequence dataSequence Downloader
      CCGTAATGTCGTGAACGGGACTAGCGGAGCCGCAGGAAGTGGGCGCAGGACGACTGGCACTGCCCACAGCAGCTTCCGGT
TCTCTGGCGCCGGCATCTTTCGGCACTCGTCGTCGTCTCCCGCCTCGAGCTGCCACTCCACCAGCACGTCGCGTTCGAGC
TCCTTCTGGCGCAAACACGGCGGCTATCCAAACCTAATGGACAGACTGGCCATACATTTAAGACTTTCTTTGGTACCACC
CAGTAGCCATCCAAATCGACGGGCATCTGTGCGTGTCACCATCTCCCAGCCACAATTGGGTTACCCGACCAATGTAGGTG
GAAATGGAGGTAGAACTGTTGGAGCGACGGAGGTGGGGGCTGGTGGCAATGGCTCATCAGCCGGCAGAACGAGCAACTCA
TCTCTTGGAGGCACGAGCTTGATGAAAATCGCAACCATCCAGGGACCGATACTGAACCAAAGTCAGGCAGCTGGAGGCAA
TCACATGGTGCAAAAGGTGAAGCCGACGGCTTTAAAACCGGAAGAACCACAGCGCAACAAAGTGCGGCCCTTTAAATTTG
CCTTCAACCGCGTTGCCACGCCCACCCTCAATTTGCGTTTTCTAAACAATCGCAGTAAACGAAACAGCTTGTCGGCCAAT
GCCGTTGCTACGGAGCAGAAGGCCACCAAGGTGCTCGGATTGGTGTTTTTCACCTTCGTTCTGTGCTGGTCGCCTTTCTT
CATCTTGAACATTATATTTGCCGCATGCCCCGAGTGCCAAGTGCCGGAGCACGTGGTGAATACCTGCCTTTGGCTTGGCT
ATGTCTCCTCGACGATCAATCCCATCATCTACACCATATTCAATCGCACGTTCCGGGCGGCCTTTATCCGCCTGCTCAAG
TGCAACTGCAAACGTTCCGGCCGCCCGCTGCGCTATCGGTCTGTGACAGAAGGACGCGGCGCTCTCAGCCTGTGCGCCCC
TTCGGCCCTGCCGCTGGCCATATCCTTCCAGGGGGCGCCACTTATGACGCCATCTACGGCTAATGCGACGCCGATGAGCG
AGTTCCGCGCCAGCTGCCGCACAGTCACAGACGACGAGTGCTGAACCACGAGGAGCACCATCCAGCGTCTGGGATGCATC
AAATTTATATCGACGTATCGGCAGAAACAAAACGGAGTCCACTGTAATTAGCGGCTTTTCCTAGATAACTTCAGGGAGAT
CAATGCTAACCTTTTCAATCACAATCAATTTGATGAATTTTGTAGATGCTATAGAGAAAATACCTAACTTAAGTCTCATC
GCATATCATTAGAAACAAAGGAAGGTTCAAAAATATAGTGAGAGAGTTTGCGGTACAACGCGGACTGTTTTTCACTTTCT
CATCAACATTCTAGACTTGGTCATTGCGTTATTTCTAAGTCAAACAAAAAATCATTAACAATAAGTACGATTAGTGCAAA
ATAAAATAAGAAAAAGAAGGTAAAAAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: HL_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL07801 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      HL_pOT2
      (Rubin et al., 2000)
      HL_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)