FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\HL07915
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General Information
Symbol
Dmel\HL07915
Species
D. melanogaster
Name
HL07915
FlyBase ID
FBcl0150975
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG8501
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI07730
Library
HL_pOT2_cDNA
  • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pOT2 vector.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    934
    NCBI
    AY089527
    Full Length sequence dataSequence Downloader
    ATCAGGGCTGGAGAGCCACCGAGATTGCGTCAGTGACCGCACGGCGAGTAAAGCATGCTTTACTAGCATAATGATCTAAT
TCACAGCACTGCGAGAAAACGCCCTCCAAAAGCAAGAGACTACACTGTCGTTTGGGATAAACTCGCATCTCGCTGGCATA
TCAGCTCGAGCTGGGTGCGGTTGTGTATTCCTCGACTTAATCACTGAAACTCGAACATACGAAAAACTCTTCACATTAAT
CGCAGTCCTCCAACTTCGTTAGCTGGCTGGGTAGTGTGCGTCCGGAGATCATGGGCAGCGGATCTGAGCTTCCACTACGG
ATCTGTATGGTCTGGCTGGTCGCGGGTCTGGCGATTGGCGAGGCACTCCGGTGCTACGAGTGTGTCGATCAGGAGACGTC
GTGCGGATCTGCTGATAATTCGCCGGGAAGAGTGCGAGAATGCCCGAACTCAACCATGTGCTCGACCACCATGCTGACCA
CAATGGTGAATGGAAATGAGTGGATCAGGGTGAGGCGAGGCTGTGCCAAGCAGGTGGATTATTACTTTGACTATATTGGA
AAGCATTGGGAGCAGAAGTATCGACTGATGGACTTACCGGAGGGCTGCAAGAAGGAGAATGGAAGGATGAATTGCAACTG
TCGCGGAGAACTGTGCAATTCAGGAAGTCATTCGACACCAAATTTCCGGTTGCCGTTCGTGCTGATCTTATTGGCCCTGG
TCTGCGGCAAAATCCTTCTTCCCGTCTAGTCGGCGACACTTTTTGGGGTGTGTTCTACCAGTTCCCGGAACTATGGAGCT
CTCTAAGGCCACAAATATAGTTTTAAGCAGATTAATGATTTATGTGCGACAGTTTTCAAAGGTGAAACCTTGTCAATAAA
GTCACTGATACCAAATAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: HL_pOT2_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL07801 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    HL_pOT2
    (Rubin et al., 2000)
    HL_pOT2_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)