FB2026_03 , released September 17, 2026
Clone: Dmel\LD13253
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General Information
Symbol
Dmel\LD13253
Species
D. melanogaster
Name
LD13253
FlyBase ID
FBcl0152978
Feature type
cDNA_clone
Computed gene(s)
Dmel\lama, Dmel\CG46456
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI19716
Library
LD_pBS_cDNA
  • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pBS vector.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage
    organism

    Comment: 0-22 hr AEL

    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,544
    NCBI
    BT001475
    Full Length sequence dataSequence Downloader
    TCGAAACGGAAGGAAAATCGCTTTAATTCTCTGTGATCTGTGTTTGCGTGTTGAACTCGAGTTTTCAGTTCGTTGTTATC
TGTTCAATTTCATTTGGATGTGCAATTGCCGACGCTAAAAATATATACGTAACGCGTGCAAGAAATTGAGAGTATTGCCC
GACGCTCAACTAAATTACAAAAAAGAAATTTTATTTCATATATAAACGCCACGTTCTCGCGTTCTAACAAACTCCCCAAT
AAAAAACACACTCAAGCAGCACATTACATATACTCGACCACGCTGTATAATTAGTTACTAGCCGTTGATATTTCCAATAC
GCCGCATAGCAACAATAACCATATCAAACATTTCGATAATCTGATGTGACACAAGTTTTTGCATCGAAATAGCCGTGTGT
GTGTGTGTGTGTGTGTTTGGTTCCCATTCGCCGCCAATCAAACCAATCGCATTGAGAGTGTAATCCGGGAAAAGTTATAC
ACACCTAAACCTGCGCCTTTTCAATGTAGCCCTTCATGGATAATTAACACGTGAGCGTTGTGTGACTATATAGCAAAATC
GTGTTAAAATCGTGTTCGCAGTGGCTGAATTTAGCAGCGATCCAACGAATAGTGTCATCTCGAACCCAGACACCCCCCAC
ACCCCCTGCGAATTTCTCTTATTTGTCGGCGCGGAATTTGCCTGAGTCACGAGCCATTTCAATTTTCCGTTCTTCAAATG
GGTTGACTTGTGATATTGCTACTTCCTGGACCCGGGAAACAAAAGGGATACAAATTGAACCTCAAAAGACATAGCCAAGC
TGCAATAAAACCATCGCCAAACCACCGCCGCCATTTAAAGTCAATAGTTAGCACGAATATAACCACCTAGAAACCGTTGG
AATCTCTGCTTGAAAGCAGCGCACCACCCATGAACCAATCGGCCAAGGCGCTCAACAGCGTGGCCACCGAGATCAGCACC
TCCTGCTCCTCCAGCACCTTCACCAACCATCCACAGAACCACCTGCACACTGGCCACCTGATTGCCCATCGTCTTGGACA
CTCCTACGCCGCTCGTGCCGATCACAATTATCTGGGATAAGTTCTGCAATTGGAATCTGACTGGTGAAACACGTTTCTCG
AGAGGATTTTCCATTTTTGCATCGTAGCAGCTTCACGACGGCCGGCACAATGTTAAAAGTCGTTGGTGCATCGTGGCAAA
AAACGCGGATAGGGACCTACATCCTAATCGGTGCTGGTCTCTTGGTCATCGGAGCCTTCTTCATTGGATATATGGAGAGG
CCGGAGTACGATGGCACCTACTGTGCCACCGCTCTGTGGACCAAGCAGGTGGGCTTCCAGATTGAAAACTGGAAGCAGCA
GAATGATCTGGTCAACATTCCCACGGGTGTTGGCAGGATTTGTTATAAGGATTCGGTTTATGAGAATGGTTGGGCCCAAA
TCGAAGTGGAGACGCAAAGAACTTATCCGGATTGGGTGCAGGCCTATGCCGCCGGAATGCTGGAGGGCTCGCTGACCTGG
AGGAACATCTACAATCAGTGGTCCAATACCATTTCCTCTTCTTGCGAGCGCGATGAAAGCACCCAGAAATTCTGCGGTTG
GCTCAGGGACCTTTTGACCACCAACTACCATCGCCTGAAGAGGCAGACTGAAAAGGCTGAGAATGATCACTACTGGCATC
AGCTGCATCTGTTCATCACCCAGCTGGAGGGCCTGGAAACTGGCTACAAGCGAGGAGCATCCCGGGCACGATCCGACTTG
GAGGAGGAGATACCATTTTCCGATTTTCTGCTGATGAACGCCGCTGCCGATATTCAGGATCTCAAGATCTATTACGAGAA
CTATGAGCTGCAGAATAGTACGGAACATACCGAGGAACCCGGAACGGATCAGCCAAAGAACTTCTTTCTGCCGAGTGCCA
CCATGCTGACGAAGATCGTCCAGGAGGAGGAGTCGCCGCAGGTACTACAGTTGCTCTTTGGTCACAGCACGGCTGGAAGT
TACTCCTCCATGTTGAGAATCCAGAAGCGGTACAAGTTTCACTATCACTTCTCGTCGAAACTGCGGAGTAACACGGTGCC
CGGTGTGGATATCACTTTCACTGGTTATCCTGGCATTCTCGGATCCACCGATGACTTCTACACCATCAAGGGTCGTCATC
TGCATGCAATCGTAGGAGGAGTGGGCATCAAGAACGAGAATCTGCAGCTGTGGAAGACGGTGGATCCGAAGAAGATGGTG
CCCTTGGTGGCTCGTGTAATGGCCGCCAATCGGATATCCCAGAATCGTCAGACTTGGGCCAGTGCCATGTCCAGACATCC
ATTTACGGGAGCCAAGCAGTGGATCACCGTTGATCTGAACAAGATGAAGGTGCAGGATAATCTGTACAATGTCCTTGAAG
GCGATGACAAGCATGACGATGCTCCAGTAGTGCTGAACGAAAACGATAGGACAGCCATCCAGCAGAGGCACGATCAGCTA
AGGGATATGGTTTGGATTGCCGAGCAGCTGCCGGGCATGATGACCAAAAAGGATGTTACTCAGGGATTCCTGGTGCCCGG
CAACACGTCCTGGCTGGCCAATGGAGTGCCGTATTTCAAAAATGTCCTGGAGCTAAGCGGCGTCAACTACAGCGAGGATC
AACAGCTGACGGTGGCCGATGAGGAGGAGCTGACCAGTCTGGCGTCGGTGGATAAGTATTTGCGGACTCATGGATTCCGT
GGTGATCTGCTGGGCAGCCAGGAGTCGATTGCCTATGGCAACATAGATCTGAAATTGTTCTCCTACAATGCCCGACTGGG
CATCAGCGATTTCCATGCCTTTGCGGGTCCAGTCTTTCTGAGATTCCAACATACTCAGCCCAGGACTCTGGAGGATGAGG
GCCAGGATGGTGGTGTTCCACCCGCCGCATCCATGGGCGACGAACGGCTCAGCGTGAGCATCGAGGATGCAGATAGTCTG
GCGGAAATGGAACTCATCACGGAACGGCGATCTGTGCGAAATGATATGAGAGCCATTGCCATGCGAAAGATTGGCAGCGG
ACCCTTCAAGTGGTCAGAGATGAGTCCCGTGGAGGAGGGCGGTGGCCATGAGGGGCATCCTGATGAGTGGAACTTTGACA
AGGTCTCGCCGAAGTGGGCGTGGTAGGCGCTGAGGAGCAGGTTTCTTCTTTTAACCCGTAGAACCCAATCGAATTTGTAC
CCAAAACCCACCTCCATCCATTTTGAAAACTGGAAAGCAATAATTGTCCTAGATTTGGTAATTAGTGTCCTGTCTTGTCT
CGTATAATTTGTGTGTTCCTAGTTGGTACATATCTTACGTCCATAGTCTTCTAGTGTCTACTGTACTTATGTATGTATGC
GTGTCCAATCGATTATTGTCTTCCAGCTGTACAGCTTTTTATTTTCCGTAGGTCTTTTGTAAATTAGCAACGAAATTGGA
AATATATTTTTTAGCAACAAATTTTTGTACTTTAGTAACTATTAACAAATTGTAAAGAAAATAAAAACAAAATCATGAAA
AACAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: LD_pBS_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

    (Rubin et al., 2000)
    Sample preparation

    Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment
    Reported As
    Symbol Synonyms
    LD
    (Rubin et al., 2000)
    LD_pBS
    (Rubin et al., 2000)
    LD_pBS_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)