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FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
AAAACGCGAATAATTAACGTGTGTTTATTGTAAAAAGACAGCGCGATTATGATCTAATCAAAGAACAACTGGATCGAGCC GAGGGCACAGCGATTGGCTCCAAAAAGTTCCCATTCTCGTAAGATTAACGGAGCTGCCTTGAAATGGAGCAGTATATTGT GCCCCTGGAGGAGCCAGTCTACTCTGATTTGCAAGTAGTGCAAGTGCCCCAAGATGAGGTTGGGTCCCTGGTCCTACAGG ATGACCAGCAGCTGGTGTTCAACGACCAGCAGCAAGTTGTCTACCAGACGACGGCCCCAGCGCAATATCAACAGCCCCAG CAGTCTGCGGCACCCACATACCACATAATCGGTGGCGATGATCTTGCACATCAGCAGCAACAGCACCAACAGCAGCAGCA GCAACAACAACAGCAGCAGCAGCAACAACAACAACCGCAGCAGCAGCACCAACAACAGCAACAACAAGTTGTAATGCATC ATCAGCAGCATCAGGTGCAGCAAGTGCAGCAGGTTCACTATCAGCAGCAGCAACAGGAGCCCCAGCAGCAGCAGCAACAA CTGCACATGGAGCAACAGCAGCAGCAGCAGCAGCAATATGTGCAGCATCAGCCGCAGCAACCCGCCATCCAGCATACACA GCAGATTTACTACACTTCAGAGCAAGTCCTGCAACCAAATGCCATTGTGCAGCATATGCAGCAGCAGAAGAAGCAGCAGC AAGTGCTGCAGCAACAGCAGCAGCAGCAGCAACAGTATCAACAACACGCACCCGCCTACCAAGTGCAATCGTCTCCCCAA GGGCAAGCCCAATTACTTCAGAGTTCCCAGCAGCAGCATCAGCAAGCGCAGCAGGCACAAACGTTGCAACTGCAGACGTC GCCACCCCAACAGCAACCGCAGCAGCACCAGCAGATCGTCTACCGCATGACCACCACACAGCAGCAACAAAGGCCCTTAG GGATGCTCAACAATACGCACGTGATTGTCCAGCAGCAACCGGTATTGATCCAGGGCCCACAGCATCAGGAGCTGATTATG CGACAGGCCATACATCCGTACAACAATCGTGTAGTTTATGCCACTTCACCGCAACAGCAGCAGCAGCAACAACACGTCAT CCAGCAGCACGTACAGCAGCAGCAAGTGCAGCAGCATCAGCAAGTCCAGCAACAACAGCAGATGCAACTGCAGCCCACGA CTCAAACAGCCCATTTAGTTCAGGCTCGTGTGGTGCCACAGCAGACCCAAAATGTTGTCTACCAGCAGTTGCAGGTGCAA AAGGTGCAGCCGCAGCAACAGCAGCAGGCTCCACAGCAGCAGCAACCGCAATTGGTGGCTCAGCAGCAGCCGCAGCTAGT GGCCACCTCTGCTGCCGGTGGCACTCAACTAGTACGCGCCTCGTTTAGGAAATCGCCCCGAGGTGGAGCTGTGCTGGCCA GGCCGGGCACACTAATTACCACCAGGCCTACACTTACGCCCACAACAATTGTGCGGCAAGTTCGTCCGCGAGGAGGTGCA GTGCCCGGCGGACTGGTTAGGAATATGCGACCTGCGGGCGTACGACCCACTCGAGCGCAGTTGGTGGTGCAGACTAGCGC CAGCGGCGAGGGACAGACCATGCTAGTGACTCAGGGCCAAATGAAGCACATTACCATCAGCACGAATCTCGGCCAGCCGC AACAACAACAGCAGCAGCAGATGCAGCAACAGCCGCGCCACATCTACCGCACACACATGGTAACCGACGGAAATCCTCAG CAGCAGCAGCAGCAGGCGGCGCAACAGCAGCCGCAGCAGCACCAGCAAATGCTGATGATGCGCCATGGTCCGGTTCGTTA CAGAGCTCCGGTGAGCTCAGCTGTCGCAGCGCCAGTTCAAGCGCCTGCTCCTACGGCCGCAGGTGGAGGTGTCCAAACTA GTAATATAGGCATGGATTTGGAAGAGCGCATAGAGGCGGCAGTGCTGAAAAAGGAGCAGCAGAAGCCCCAGCCAACGGCT CAGCCAGTGGCCTCCAATCAACTACAGCCCATGCAGATAGGAACGGGAACTACACTGACCACCTACTATCCAGCAGGAAC TGCCTCACAAGAAGAGGAACAGCAACAGCAGCAGCAGCAACAACAACAGCAGCAGCAGCAGCAGCAGCAAGTGGTGCAGA GTCAGCAACAGGGAATACGACCACCCAGTGGAGCAAGCATGACTTTGGCGGAGTACAAGAAGAGGCAAACCCTGTCGGCC TCACCGGCTCCAGTTCCGCCTTCAAATCCCGGACTTGCGCCAATGAGAGCCACTCCACCTGGAACCATGGTTCGCCCAAC GGCTAAGATAACACCCACGCAACATGCTGGCGCCTTGCCCCCCGGCACTAGGATCATGCAGCCACAGAGGCCAGTACCAG CCGCAGTGGCGGCTCCAATTCCAGCGCCAGCGGCGGTCACGCAAGGATCATCCGTGATGAAGACATCGCACAATAACTAC GAGATTCACTCGCAGCATGTTCTGAATAACCGAGCCGGTCAGGAAATCGCCGAGCGCGATAAGAATAGTGCCAAGATGCT GGTGATCCTGGCCAGCGGAGAGCAACGTCTGATCACCTTTACGCTTCCACGTGAGTCCTGCACCGTGCAGGATCTCCTCG AGCAGGTCGGCGTGCCCTTTGACCACAACACCACCATTCAGTGTGTCGAGCATCGCGGTGCCAATGTGGACTTTGTGGTC ACCGTCGGATTTACGGTGCACGAGTCGGCCAGCGAACTGATTTCACGGGCGGAGGAGAGTCTGCAAATGAACCGTTCGCA GGAGAATCAAGCTGCCGTCGCCGGAGCAGCAGCAGCAGCCAATTCCAATCCTGCCACAACGAGTGCCTCGCCTGCCTATG CCCAAGCGAATGCCGCTGCCGAGCAGGCGAAACGCGAAGCGGCCAGTTCGGCAAGTGGGAACACAGTTGTTGGGACAGTT TCCTCCGCCGGTTCCGCTGGAGCCACTCCGTCGGAGGGCAGGAAGCTGATCGACGGTTTTCTGGCCGTCTGCCAGCTGTG TGGATTCACTGGCGTGGATCACGCCCGCTGCGAGCGGTGCAAACGCGTCTTCCCGGAGCCGCCGAAACGCAAATCCTACG TGCCCAAGAATAGTGCTGCATCCTCGCCAGCCTCGTCGTCATCCAGCGAACCCGTATCTGCTGTCCCAACGGCTGCGGAT AAGAAACGAGAGTTGGCGGCAGCTAGATTCAACAATAAAATGGCTGGAGCTAACTTCGCAGTCGGAGTTGGAGTTCGCGG ACGCGGATCCATGGCCATGAGAGGTGGCAGAGCCAGGGGAGGCAGACGGGTGCCCGAAGTGGATCCCGTGGTGCTGCTGA GCAGCGAGGATGAGGCCGAGGGGGGCGAAGGCACCTCGGACTCGAGCAACCAACCAACAAACAACTCCAAGCTGAACTCC AATGGAAATCTCCACGAACTTTCCGCCGCCTTTCCCTGCGAGCCAAATCTGCCAGAGATCGATGAGGAGCCCTTGTACAA AGATTTCTCCCGTGGCGATGTTAGAGACTTGTCGGATATACCCGAAGCGGATCAATTTTCCGCCTCCTTGAAATGCAGCT GCGTGCGTCTGGTGCCCTACAGATTCGAGATTACCGAGCCGGTATGCTTCACCACGAAGGGCGTTCGCATTGCTGGCATC CTGAAGGACAAGGACGAGAAGTTCGAGCTGAACATCCACAAACAGGAGGTGATCAAGGTGATAGCCCACTTCGGCAACGT TGAGCCCGCCCAGCCACTGGTCACGCTATATCTCCTCAAGACGTGTGCCGAATACGTAAAGAACCAGCTCAAGCTGCCCG ACGATCAGCATCACGAACATACCGGCTTCAAGACGCACAGCTATCACACTCGCCGTCTGATCCTGTTGTTCGACAGCATA TCCGTGTCCGCCCGTGGTAATATCAAGTCTATGTTCAGTTGTGTAGACGAGATATCCTCGACAGATGCCGCCGAGATTCT CGAGAGGATCGCGGACTCCGATCGCAAGACGCAGGACAAGAGCTCGCAGCCACCGCCTCGCCAACTGAGAGCAGACGAGC AGGTTAGTCTGCTGATGTATCCGCCGAAGGGAACGGGTGGCCTGTGCATACGCATGGAGGACTTCGTCTGCCTCACAAAG GAGTCCTACCTCAACGACATCATCATTGACTTCTATCTGCTCTGGCTGAGGAACACGCTGATTCCGGAGCCTCAACGCGA AAGAACGCATATCTTCAGCACATTCTTTTACAAGAGACTCACAACGCTAACGCGGCCAGCGGACATGAAGCAGACGGCAG CACAGAAGCGACACGCCAGGGTGCAAAAGTGGACCAAGGGTGTGGACATCTTCGATAAGGATTTCATTATAGTGCCCATC AATGAGCAGTCGCATTGGTTCCTCGCCATCATTTGCTACCCGAACTTAAAAGGACCCGTCACGTTCGATACGAACCAGCC TGTAGAGCCGCAGCAGTTGAAGCGCCCTCGAGGCAAGAAGGTGGCCCTGCAAATTGGTAACACCACCATAACCCCGCTGA CGAAAAGGGCAGAGGGAGGACAGCTTCCAGCGGCTCTGACGGCAGAGAACGTGTGTCGGATAGCAGACGATGAGAGCGAA AGGGATGAGGCCGAAGGCGATGAGAGCGACATGGCCTCGGAGGATAGTGAAAACTCGAATTCGGCCAAGGAACCAGCGGC CACGCCCACCTCCACGACAGCCAGCACCCCCAGTCTATCCAGTTCGCAGCCCACAGCCACCGGTCCAGCGAGGAATATTG CATCCGATGATGTGCCCGCCGTAAAGCAACCTTTAATCCTTATCTTTGATTCCCTGGCGGGAGCATCGCGTAGCCGCGTG GTGGCCACACTGCGAGATTATCTCACATGCGAGTACCGGGTAAAGAAGCCGGATGCGCAGGCGCACGTCTTCAACAAGGA CAATATGCCGGGCCACTGCGTCAAGGTGCCGCAGCAGAACAACTTCACCGATTGCGGCCTGTATCTGCTGCAGTATGTGG AGCAATTCTTCGGCGAGCCAATCAGAGACTACAGACTGCCCATCAAGCAGCTGACCAACTGGTTTGACTTCCTCACAGTG ACGAAAAAGCGCGAGGACATTGCCAATCTCATCCAGCAGCTGATGGACGAGGGCAATCAGCAGCAACGCCTCATACTGCC AGTGATCGAGTTCCCAACGCTGAATGGACAGTTGGTGGAGTATCCAGAGGACACGGAAAGTGCCGAGTTTGAGGAGGAGG AGGGCCATGACGACGAAGATCCAGCTAGTGAAATGCATGATGAAAACAATGCCGGCACCGACATGGAAGTGGACCCCGTG AACGAGGAGCCAGCGTTGGCAGGGAAAACCGCCGCGTTAGCACCTGCGTCCACGACTGCGGCAGCGCCCACGGGTAAAAG ATTCGTGCTGAAGCGGCGCCTGCACAACGGAGCCGTCTCAAGTCCGACCAATGGAAACGGCGAGGCTAGCAGTAATGGCG GTGCACTGATACCCCAGCTGGTGAGCACGGCGGCCGCAACGACGACGGGCGTTTCGTCCGGAGTCGCTCATTTGGGGCCG CGTGGGCCCAGCGGTGGACTGAAGATCCGGAAGATAGAGCCGTAGCACCAAACGATTCTCGCCCTGCGAGCTCGCGATCC GGATGCGCACATGCCCCACCTGCCTGCGCTGCTCCAGTTGCCACAGCTGCGGCACCACCTGTAAATACGCCATTACATTA CATCGGTTATTCAATTCTATGTATCTAAACGTTTGACTCTAAAAAACTACACTAAACTAAAACTATAAATAAAGACTAAA TTACTATGTATGAAATACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.