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FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
AAATCCAAGTGTGTGTGTAAAGGAAACTGTGTGTGAAAAATTTGAGTTTGTTACCAATTTGGAAATACCGACGTTGGGGC TACAAAATACAGGAGCATTGCCCACTGGCCACAGCATCTGCCCTTTAAACGAGTCATCACGGAAGCTGTTGAGCGATAAA CGCCAGGCGGAGTTCCCGAAAATTGGCAATTGATGCCACTCACGGAAATCGGCACTCGGCACCCTGCACCCTGCACTTTT CGGTCACAGCGGCCTGCAATTACAGACAAGCCAGGCCGAAAGGCGACTACGCGGACTCCGGCTGGGCATCGTATCGAAAA TGTGGCTACCAGGCCGAGAGCAGAGACTAGCAAGAACGAATAACTAGATGCAAATCGTGCAAATAGCACTCCCTATATGT AATATATATGTAATACACACGCGCCTGCCCTGCCGACAGTGTACACACAACAATATATCGGCTTGATACACACATACGCA GTTGCATCAGCATGTCCGACCGTGATTCCGATGCCGTATCCGACGCTGGTCCTCGCCTGAGTCAGCAGCGCGGGCAGCGG AACCGGCGCATCATAATGCGGATCGAGTTCAAGGACTCGGAGGATTCGGCGTCGGATCAAGACCGGGATGACCGCCCCTC CTGCTCCGCGGCGACGCGCCTGAGACGCAGCACCCGTCGACAGGAGGTGGCTTCGCATTCGGATTCCGACTCCGTGCCCC GGAAGTCTACGCGGAAGCTGCGCAATCGCTACGGGCCGCGGAGCGATTGCTCATCCAGCGGCAGCGATATCGATCAACTG CCGACGAGAACCAGAAAACGAAAGAAGGTAGAGTCCTCCGATGAGGAGGAGCCCGAGCGGACGCCCAACGCGGTTGGAAG AATCCAACCCATGCCCGCTGACCCCGACGACGGCTTCAGTTCCGACATCAGCAGTAACGATCTGCTGGAGAAGTGCCCCA TTTGCTTGCTCACTTTCCGGCAGCAGGAGATTGGTACGCCAGCAACATGCGAGCACATATTCTGCGCGGCCTGCATCGAC GCCTGGTCCCGAAATGTCCAAACTTGTCCCATTGATCGCATTGAATTTGACCGGATTATCGTGCGGGACTCGTACGCTAG TCGCCGGGTGGTGAGAGAAGTGAGGTTGGACCTGAGTAAGTCCAATACTGAATTGGTGGTAGATGACGAGGCAGACACCG CTGCGCTTTCCGAAGAGGAGGTCACCAACTGCGAAATCTGCGAAAGTCCGGATCGGGAGGATGTAATGTTGTTGTGCGAT AGCTGCAATCAGGGATACCACATGGACTGCCTAGACCCGCCGCTGTACGAGATCCCAGCTGGGTCGTGGTATTGCGACAA CTGTATTGACTCCGACGACGAGGATGATAATGAGCAGCTGGAGCTGGCTGACGACTTGGATCAGCTATACGAGGATATTA GAGGTATGGGCTTGCCGGAGACTCGTCTCAGGGTACGTGAGGTGCAGGAACCGCCAAGAATCCTGCGCACCCGTCAAAAC GAACGCATAAGGGCTGCTGTACTGCGACGTACTCGCTCGGGCGCTACGTCGGCGGAAGATTCCACACTGACCCGGACTCG TACCCGCACCACCACCACAACAACAACCACTACTACAACTACTCGGCGCACAACCACAAGCACGAAAAAAAGACCTGTTC AGCGGCGGCGACGGAGAAGGACTCGCTATCGCACCTACGTGGTCGAGTACGATCTAAACAACTTTGACGAAAAGTTCGCC CTTAAAACCAGCAAGAAAGTGATAAGACGGCGTAGGCGTCGGCGTCGTCGTGGAGTCGCCACTGCCTCTAGTGAGGGGCC TGGTCGACGTCTTTCAGCCAGCAAGCGACTTGCAGAGCAGATGGGAGTAAAATCGGAAGGATTTCAAAGTTCTCATCTAA GTGGTGGAACAGCCTCCAGTTTCTCGCTATTTGGCAATGCGAACGACCTGGAGTATTTCTCCGATAGTGAGCCAGACGCA GTGAGCCAGATCAGTTCTGCTGGACTCGGATCGACTGCAGTGCAAACCTCAGTGCGCATTGGAAGCATTGGAGCACCGCG CATCCGTAAAGCCTTGCTCCAAGGCAAGGCGCGCATAGCAGCGCCGCCCACAGCTCCGACTTCAGCTCCCGCTGCAGACA TACTGTCTAGCATTTTGGATTTGCAGGATCGCTGGCACGATGCTTCACGTAACCTAGGCGAAGTGCATATTAGGGCGGAC GGTACCCTCAATATTCCCCAGAGACCTGCACCTACAGCGACGGAAGCGCCTTTACCGAAAGCAGGTACTTCTAAGACCGA GAAGCATTTAACCCAGGCACCGCTTTACCAACAGGGAGGAGGAGGACCTAACTACAACAGAGGCGGAGGAGCAGGCTCAA GTGATAGTTACAACAATCGTTACAGCGGCAATACCAGTTACCGCGGAGGTGGTGGAGGCGGCGCTGGAGGATCTAGCGGT GGTAGTGGTGTGCCCAGCAGTAGCGGGGAAGCTTCCTCGCGACACTTGACCGGTGAAGGAGACGGCCAAGGAGGCGGTGG CTTTGGCAACCAAAACTTTAGCAGCTCCGGCTCCAACAACAACACTAATATGTCAAGCTTTATTCCGTTCCATCTGCGAT TCAACACGCCCACCCGCCAGCAGCAACCACGACAGCAAAACGTGCTGCCAGCTAACCAACCCACCCCGCCTTTCCCTGGA AGTGCACCGCCTCCAGCCCCAGTAGCGTCGTCATCAAACAATTTTTCGGGACAGACTCCAATGTTTGCAGCTCCCCTCAA TCATCATCCTGCACCAGTGATGGGTATGCCAGTTGTGCTGTCCATACCACCTCCGCCTATGCCACCTGCCAGCCTTCCAT GGAACAGTCCTCTCTTCAAGATAACCCCACTCCAGCAAGCACCGGCCAAATCCAACGATGGCAACCAAAACGACGATGTT GACAACTGCCCAAACTTCTCAATTTACTCGCAGGAGTCGCAGGCGGTTGCTAATGCCTCGGCGATGCCGTCAGGGGTTCC ACACGGACCAGCGGATGCATCTGATAAGGATGATGACGATATGAATGAAGATTTAGTACAATTAGATGATGATGATGAGG ATACGGATATACCATTGCCTTTGGGGCCTGAGCCCGAACCCGTTCCCGAAAAAGTCCCGAAAAGTAGTGATGATGACCTC TACGAGCCAGAGAATCCAACCGAAGAACCTGAAGAACCCGAAATGTTCGAAGAGTCCTGTGACGCTGTTCCAACGGAGAA GTCCGAGTCCTCAGATCACGAGCCAAGCAACTCCAACGTGCAAGCTGCAGCCCCAGAGGAAAACGATGCTGAGGAAGCTA GGACGCGCAGCACTCCCAGCCCACTGGTCAAAGAGGATCGCGCTGCGGATCGAGATCGGGCCAAGGGAGTACTCGAGCTT TATGATGATAGCGACTGGGAGGAGTTAGACATTGACAAACCGAAAGAGTTCGAGAAGGCACTGCTTACGGAAACGGAGGC ACAGGTCAGCCCCAAGAGAGCTCCGTCTAAGCGACCACCATCAAAAAAGGATACAAGCTTAGAGAGAGATGTCAACGAAA GCGGTAGCGAGCACGAGCAGGATCGCTCGTACACGCCCTGTCTGGACGAAAAAAATTTGGCAGAGGAGGAAGAGGCTGGG ACAACGCATCAAAACAATGTAAGTACGAGTCCCAAGACTCGATCCTCTAAAGTTTCCGGCGACGAGGATGAGCCAGAGAA AGCTGTCGGTACGGATATTCAGTCAGAGGACGAATTGAACAACGAAAACGAGTCACCGCGACGCAGTCGAAGCCGTGGCA CGGCTAACAATAAAAAAGACAAGTCAAAGTCGCGTTCAAAACGCAACAAGGGCGAGACTTTCAAAAAGGTCGGCAAGCGG CAAAAGGATCGCAACTATCGTGGAGACAAGTCGGAGCCAGATCGGAGCCCGCGTCCAGACTCCAGGAGTCTCACTCCAAA GATGCGTTCTAAGAGCCCGAGAGTCAGCAAGAGCATTAGTCGCAGTAGGAGTGGAAGCAGGAGCAGATGTAAGAGCAAGA GTTTTAGTAGAAGCAGAAGTCGTTCCCCAAGTCGCAGTAGGAACAGGCGTCGACGTAACAGCCGTTCCAAGTCCTACTCC CGCTCTCATTCTAGGTCTCGATCGCGTTCGAACTCGCATAGCCGCCAGAGATTTTCCTTTCGCGGAAGGGACCACCAGCG CGGCTTTTTCCGTGGCCGTGGAGGCCTCCGTTTAAACTACCGCAACCAGCAGTTCCACAACAGGCCGTTCCAGCATTACG GCCAAAATTATCACCAGAACCAAAACTACCACCATCAGCACAATCAGCACCAAAATTACTTTCCACCCAATCAGCATGGC CAGCATCAGCAGTACTTTCAACGTCCCAAACGAAGGGAACTACCTCGCTATGATGTTCGAAATGTGGTGGGCACATCGAG ACATCAGACACAGGCCAAGGATCGCTATGGACGCGACGCAATGCGGTCGCACAGAAGTCGTTCGGGAAGGCGAAACTCAC GCAGTTTCTCACGTAGTGTTTCTCGTGACTCGCGGGTCTCCCTGCGCTCCCGATCTGGTTCCCCCAAGCGACGCAGCTTT AGCATGTCGCCCACGCCACCGCCAGGAACGTCACCTCCTCCTCATAAACATGGTAGGGGCCGTCAAACCTCTACGAACAG ACGCTATGCACGCTCACCCTCACCGGCACCGTCTCCCCGCAATGAAAGGGCGACTAGTCCAGTTCAATCAGTTCACTCCA GAAGATCGCGCTCGCACACTCCCAACCGCATCAACGGAGATGGTAATAGATCATCCCTATATATGGGCTCGCCACACTAC ACGCCACGGATAAGTCGCAGCCGCAGCAGAAGTCGTTCCAAGAGTCCCAAGGATATGCACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.