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FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
GAAACTGTGTGTGAAAAATTTGAGTTTGTTACCAATTTGGAAATACCGACGTTGGGGCTACAAAATACAGGAGCATTGCC CACTGGCCACAGCATCTGCCCTTTAAACGAGTCATCACGGAAGCTGTTGAGCGATAAACGCCAGGCGGAGTTCCCGAAAA TTGGCAATTGATGCCACTCACGGAAATCGGCACTCGGCACCCTGCACCCTGCACTTTTCGGTCACAGCGGCCTGCAATTA CAGACAAGCCAGGCCGAAAGGCGACTACGCGGACTCCGGCTGGGCATCGTATCGAAAATGTGGCTACCAGGCCGAGAGCA GAGACTAGCAAGAACGAATAACTAGATGCAAATCGTGCAAATAGCACTCCCTATATGTAATATATATGTAATACACACGC GCCTGCCCTGCCGACAGTGTACACACAACAATATATCGGCTTGATACACACATACGCAGTTGCATCAGCATGTCCGACCG TGATTCCGATGCCGTATCCGACGCTGGTCCTCGCCTGAGTCAGCAGCGCGGGCAGCGGAACCGGCGCATCATAATGCGGA TCGAGTTCAAGGACTCGGAGGATTCGGCGTCGGATCAAGACCGGGATGACCGCCCCTCCTGCTCCGCGGCGACGCGCCTG AGACGCAGCACCCGTCGACAGGAGGTGGCTTCGCATTCGGATTCCGACTCCGTGCCCCGGAAGTCTACGCGGAAGCTGCG CAATCGCTACGGGCCGCGGAGCGATTGCTCATCCAGCGGCAGCGATATCGATCAACTGCCGACGAGAACCAGAAAACGAA AGAAGGTAGAGTCCTCCGATGAGGAGGAGCCCGAGCGGACGCCCAACGCGGTTGGAAGAATCCAACCCATGCCCGCTGAC CCCGACGACGGCTTCAGTTCCGACATCAGCAGTAACGATCTGCTGGAGAAGTGCCCCATTTGCTTGCTCACTTTCCGGCA GCAGGAGATTGGTACGCCAGCAACATGCGAGCACATATTCTGCGCGGCCTGCATCGACGCCTGGTCCCGAAATGTCCAAA CTTGTCCCATTGATCGCATTGAATTTGACCGGATTATCGTGCGGGACTCGTACGCTAGTCGCCGGGTGGTGAGAGAAGTG AGGTTGGACCTGAGTAAGTCCAATACTGAATTGGTGGTAGATGACGAGGCAGACACCGCTGCGCTTTCCGAAGAGGAGGT CACCAACTGCGAAATCTGCGAAAGTCCGGATCGGGAGGATGTAATGTTGTTGTGCGATAGCTGCAATCAGGGATACCACA TGGACTGCCTAGACCCGCCGCTGTACGAGATCCCAGCTGGGTCGTGGTATTGCGACAACTGTATTGACTCCGACGACGAG GATGATAATGAGCAGCTGGAGCTGGCTGACGACTTGGATCAGCTATACGAGGATATTAGAGGTATGGGCTTGCCGGAGAC TCGTCTCAGGGTACGTGAGGTGCAGGAACCGCCAAGAATCCTGCGCACCCGTCAAAACGAACGCATAAGGGCTGCTGTAC TGCGACGTACTCGCTCGGGCGCTACGTCGGCGGAAGATTCCACACTGACCCGGACTCGTACCCGCACCACCACCACAACA ACAACCACTACTACAACTACTCGGCGCACAACCACAAGCACGAAAAAAAGACCTGTTCAGCGGCGGCGACGGAGAAGGAC TCGCTATCGCACCTACGTGGTCGAGTACGATCTAAACAACTTTGACGAAAAGTTCGCCCTTAAAACCAGCAAGAAAGTGA TAAGACGGCGTAGGCGTCGGCGTCGTCGTGGAGTCGCCACTGCCTCTAGTGAGGGGCCTGGTCGACGTCTTTCAGCCAGC AAGCGACTTGCAGAGCAGATGGGAGTAAAATCGGAAGGATTTCAAAGTTCTCATCTAAGTGGTGGAACAGCCTCCAGTTT CTCGCTATTTGGCAATGCGAACGACCTGGAGTATTTCTCCGATAGTGAGCCAGACGCAGTGAGCCAGATCAGTTCTGCTG GACTCGGATCGACTGCAGTGCAAACCTCAGTGCGCATTGGAAGCATTGGAGCACCGCGCATCCGTAAAGCCTTGCTCCAA GGCAAGGCGCGCATAGCAGCGCCGCCCACAGCTCCGACTTCAGCTCCCGCTGCAGACATACTGTCTAGCATTTTGGATTT GCAGGATCGCTGGCACGATGCTTCACGTAACCTAGGCGAAGTGCATATTAGGGCGGACGGTACCCTCAATATTCCCCAGA GACCTGCACCTACAGCGACGGAAGCGCCTTTACCGAAAGCAGGTACTTCTAAGACCGAGAAGCATTTAACCCAGGCACCG CTTTACCAACAGGGAGGAGGAGGACCTAACTACAACAGAGGCGGAGGAGCAGGCTCAAGTGATAGTTACAACAATCGTTA CAGCGGCAATACCAGTTACCGCGGAGGTGGTGGAGGCGGCGCTGGAGGATCTAGCGGTGGTAGTGGTGTGCCCAGCAGTA GCGGGGAAGCTTCCTCGCGACACTTGACCGGTGAAGGAGACGGCCAAGGAGGCGGTGGCTTTGGCAACCAAAACTTTAGC AGCTCCGGCTCCAACAACAACACTAATATGTCAAGCTTTATTCCGTTCCATCTGCGATTCAACACGCCCACCCGCCAGCA GCAACCACGACAGCAAAACGTGCTGCCAGCTAACCAACCCACCCCGCCTTTCCCTGGAAGTGCACCGCCTCCAGCCCCAG TAGCGTCGTCATCAAACAATTTTTCGGGACAGACTCCAATGTTTGCAGCTCCCCTCAATCATCATCCTGCACCAGTGATG GGTATGCCAGTTGTGCTGTCCATACCACCTCCGCCTATGCCACCTGCCAGCCTTCCATGGAACAGTCCTCTCTTCAAGAT AACCCCACTCCAGCAAGCACCGGCCAAATCCAACGATGGCAACCAAAACGACGATGTTGACAACTGCCCAAACTTCTCAA TTTACTCGCAGGAGTCGCAGGCGGTTGCTAATGCCTCGGCGATGCCGTCAGGGGTTCCACACGGACCAGCGGATGCATCT GATAAGGATGATGACGATATGAATGAAGATTTAGTACAATTAGATGATGATGATGAGGATACGGATATACCATTGCCTTT GGGGCCTGAGCCCGAACCCGTTCCCGAAAAAGTCCCGAAAAGTAGTGATGATGACCTCTACGAGCCAGAGAATCCAACCG AAGAACCTGAAGAACCCGAAATGTTCGAAGAGTCCTGTGACGCTGTTCCAACGGAGAAGTCCGAGTCCTCAGATCACGAG CCAAGCAACTCCAACGTGCAAGCTGCAGCCCCAGAGGAAAACGATGCTGAGGAAGCTAGGACGCGCAGCACTCCCAGCCC ACTGGTCAAAGAGGATCGCGCTGCGGATCGAGATCGGGCCAAGGGAGTACTCGAGCTTTATGATGATAGCGACTGGGAGG AGTTAGACATTGACAAACCGAAAGAGTTCGAGAAGGCACTGCTTACGGAAACGGAGGCACAGGTCAGCCCCAAGAGAGCT CCGTCTAAGCGACCACCATCAAAAAAGGATACAAGCTTAGAGAGAGATGTCAACGAAAGCGGTAGCGAGCACGAGCAGGA TCGCTCGTACACGCCCTGTCTGGACGAAAAAAATTTGGCAGAGGAGGAAGAGGCTGGGACAACGCATCAAAACAATGTAA GTACGAGTCCCAAGACTCGATCCTCTAAAGTTTCCGGCGACGAGGATGAGCCAGAGAAAGCTGTCGGTACGGATATTCAG TCAGAGGACGAATTGAACAACGAAAACGAGTCACCGCGACGCAGTCGAAGCCGTGGCACGGCTAACAATAAAAAAGACAA GTCAAAGTCGCGTTCAAAACGCAACAAGGGCGAGACTTTCAAAAAGGTCGGCAAGCGGCAAAAGGATCGCAACTATCGTG GAGACAAGTCGGAGCCAGATCGGAGCCCGCGTCCAGACTCCAGGAGTCTCACTCCAAAGATGCGTTCTAAGAGCCCGAGA GTCAGCAAGAGCATTAGTCGCAGTAGGAGTGGAAGCAGGAGCAGATGTAAGAGCAAGAGTTTTAGTAGAAGCAGAAGTCG TTCCCCAAGTCGCAGTAGGAACAGGCGTCGACGTAACAGCCGTTCCAAGTCCTACTCCCGCTCTCATTCTAGGTCTCGAT CGCGTTCGAACTCGCATAGCCGCCAGAGATTTTCCTTTCGCGGAAGGGACCACCAGCGCGGCTTTTTCCGTGGCCGTGGA GGCCTCCGTTTAAACTACCGCAACCAGCAGTTCCACAACAGGCCGTTCCAGCATTACGGCCAAAATTATCACCAGAACCA AAACTACCACCATCAGCACAATCAGCACCAAAATTACTTTCCACCCAATCAGCATGGCCAGCATCAGCAGTACTTTCAAC GTCCCAAACGAAGGGAACTACCTCGCTATGATGTTCGAAATGTGGTGGGCACATCGAGACATCAGACACAGGCCAAGGAT CGCTATGGACGCGACGCAATGCGGTCGCACAGAAGTCGTTCGGGAAGGCGAAACTCACGCAGTTTCTCACGTAGTGTTTC TCGTGACTCGCGGGTCTCCCTGCGCTCCCGATCTGGTTCCCCCAAGCGACGCAGCTTTAGCATGTCGCCCACGCCACCGC CAGGAACGTCACCTCCTCCTCATAAACATGGTAGGGGCCGTCAAACCTCTACGAACAGACGCTATGCACGCTCACCCTCA CCGGCACCGTCTCCCCGCAATGAAAGGGCGACTAGTCCAGTTCAATCAGTTCACTCCAGAAGATCGCGCTCGCACACTCC CAACCGCATCAACGGAGATGGTAATAGATCATCCCTATATATGGGCTCGCCACACTACACGCCACGGATAAGTCGCAGCC GCAGCAGAAGTCGTTCCAAGAGTCCCAAGGATATGCACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.