- Tools
- Downloads
- Links
- Community
- About
- Help
FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
CTCATTTCGCGTAATATTAGTGTAAATTCCCATAGCTCCTAGTGTTGACCAGATTGTGAACGTTGTGCCAGACGTCTGTT AATTAGCATATAGCACAACGAATATATATACCAAAAACCCGGCAAAATTACAACTCATCTCCGACGCAGTAGCCAGTTCT TTGTTACTGCTGCTCGCGCAAATAACGGTAAATGTGGATAACGGTGGATAAATCACTGCTGACCTCGACCTAGACAACAA ATTTGTACATAGCTATGTACATTGTATAAACCGAAAGCGACAAACCGATTTGTTGTTGCCGGCTATGCATTATTGATTTT CAACATCCAATTCGACAGGAGAGCGTTGGACAGGGGGGAGTGGAGCGGAGAAAGCCAGTGAATCAGTGCCGCAACGTAAC GGTAAACCCCCGATCCCGGCCACCTTAGAACCAGTACCCATCCCAAAGTTATGAACCGCACCAGGAGGAAGGTGGTGCGC CCAAAGAATCCCTTCACCAAAGCCAACTTCTTCTCACAATGGAGCTTCTGGTGGATGCGAGACCTGTTCAAGCGAGGCCT CCAGGGACCGCTGACGGACGAGGAGCTGTATCAACATAGAAAGACCCTGGATAGTGAAAGAGTGACGAACAAATTTGCGG AACTGTGGGAGGATGAGCTAAAACGAAGTAATCCCAGTGTGGTGAGGATGATCCTTCGAGCATATGGCAAGATTTTCGTG CCCATGGGGCTGGCGTTCTCCATAAGCGAAACGATTTGCAAATCTCTCATGCCGCTATTCCTGGGCGGTCTGGTCGGTTA CTTTGCCACAAATCAGACGGATATCAGTGAAAACACAGCATATTTGTATGCCATGGGAATTGTGATTTGCATGCTGGTGC CTGTGATAACGTTCCACCCTTTCATCTTCTACATATTTCAAGTGGGCACCAAGCTGCGGTTGGCCCTTTCGGGTCTCATC TACCGCAAGGTCCTGCAGGTCTCGAAGAGCAGCAGTAACGATGGTCTGCGAGGAAGAGCAATCAATATTTTGTCCAACGA CCTTGGGCGCTTCGACGTGGCGCTGTGTTTCCTTCATGATACATGGAAAGGGCCGATGGAGTCGATACTGATTGGCTTTC TCATGTACCGTGAAATCGGTATATCGGCTGTGATCGGTGTGTCCTTTATGCTCAGCTTTATCCCACTGCAAGCGTGGGCA GCCAAGAAAGCCGCCTACTATCGCCAAATGACGGCAGAGCGAACGGATATGCGTGTCAAGCTGATGAACGAGATCATCCA GGGCATTCAGGTGATCAAGATGTATGCGTGGGAAAAAAGCTTTGCCCGGGTCATCGCCGAAGTTCGCCTCAAGGAGGTGA AGGCCATTCGAGGCACTGCCTACATCCATGCCGCTCTTGGTTGCACCTCCATGATATCGCCGCTATCTGTGTTCCTGGCC CTCTGCTCCTACGTTTACCTTGGTGATCCCTTGACCGCCCAAAAGGTTTACACAGTTTCATCCTATTTCAACATGCTTAA CGATTCTATGGTAACCTTTTGGCCTATGTCTATCACGTTTATTGCCGAGGCCTTGGTCTCGGCGAAACGTTGCAAAGATT TTCTACTAGACGGAGACAAAGCGGAGGTGCCCATAAACTTGGAAGCAAATCAACAAACAGGCAAAAACAAGCGCAAAATC AGCAAAAAGGACAAACAAAAGAACGTAGAGTTAAACGGTTCCTTGCTGTCCAATGGCCAAGTCCACCACAATCGTCTAAG GGATTATGATCCGGAGGCAACGAAAAAATGTGTGGTGCTTAAAAATGTATCTGCTTCCTGGGACACCAGTGATGGTCATG CCAACTGTGCCATCGAGGACTTTAGCACGGACATAAAGGACCAAACTCTGGCTGCTGTCGTTGGACCTGTGGGTGCCGGC AAGTCCAGCTTTTTGAACGTCCTGCTTGGCGAAGTCGGCATCGATAAGGGTGAAGCCATGGTCCACGGCAAGATCTCGTA TGCCTCACAGGAAAGCTGGGTCTTCGAGGGAACTATTCGAGACAACATTGTTTTTGTTGAGGATTACAACGAGAGACGGT ACAAGAAGGTGGTGAAGGTGTGTGGCTTGGAGCGGGATATGGAGTTGTTGCCTCGCGGAGATTTGACAGTGGTTGGAGAG CGAGGAGTGAGTCTCAGTGGTGGGCAAAAGGCGCGAGTGAGTTTAGCTCGAGCAGTCTACCGCAAGGCGGACATATATCT TCTGGATGACCCTCTGTCTGCTGTGGATACCCACGTTGGCAAGCACATCTTCGATCGCTGTATACGCGACTTTCTGTCCA ACAAGATTAGAATTCTAGTCACCCATCAGGTGCAATATCTGTTCGATGTGGAGCACCTGCTGTTGATGGGGTCCGGAAAG ATTATGGCCCAGGGCAGCTACCAGGAACTCCAGCGCTCCCGCCAGTTTCAATTCCTAGAGCAGACGCAGCACGACGAGAG CGGCATCGATACTCACAGTGTCAGCAGCTATTTGTCTCGTAGCGATTCCGAGAAGAGCATGGAGCATCAGCACAACCAGC TGCTTCGACCGGACGAGCATGTAGAGGACGTCAACCAGGAGCAGCAAACTGTGGGTTCCATCAAAATGCACGTCTACGCC TCATACATCAAAGCCTTGGATAGTACCTTTTTGTTGGGCATTGTTATTTCTTTGTTCGTCTGCGCCAGGATTATGTTAAC CGGAGTGGATTACTTCTTGTCTAGATGGGTCATTTGGGAAGAGCAAATAGCTGTTAATCGCACTACAACGCTACTAAACG AAACCGACACGACGATTGCCAATGATACGCTGCCGATTAATGCCACGGATCCATATATAGCGCCTTTATTAGCTAGCGAT ATCCAGGCAGGCATTCGTCAGGAGCTTGTACTCTTCTATGCCACCATACTGGGCGCCACACTGATAGTCTACCTGATTCG CACCTTTGGCTTCTTCAAAATGTGCCTGCGCATCTCTCTGCACCTGCACGACAGGCTCTTCCGGGGAATCACTCGGGCCA GCATGTACTTCTTCAATACGAACTCCTCAGGACGAATTCTGAATCGCTTCTCCAAGGATATAAGGACAGTGGATACGGAT CTGCCGCACACACTGCTGGACTGCCTCGCCTTCATTATCGATGTTTCTGGCGTTGTCATTATTGTGGCGATTGCAAACTA TTGGCTCCTGATTCCAGCTGCCATCATTGGTGTTATTCTGGGCATGATACGCTACCTCTACGTAAACACCAGTCGCAGTG TTAAGCGCTTGGAGTCTATATCTCGCAGTCCTGTTTTCTCGCTGACTAACCAAACCTTCCAGGGTCTCACCACCATACGC GCTTTGCAGGCCCAAAGCGCCTTGGAGCAGGAGTTCCATGAGTACCAGAACACCAACACCTCCGCCTGGTTTCTCTTCCT TTCCTGCACTCGTGCCTTTGCCTTGTGGTCAGACGTGCTGTGTATCGTCTATATGACGGCGGTAACATTCAGCTTTCTGC TGCTTAAGGACGAGTTCGACAGCGGCGACGTGGGATTGGCCATTTTGCACTCCACCACGATGACTGGAATGTGCCAGTGG GGCATGCGGCAGACAGCGGAGCTGGAAAACCAGATGACCAGTGTAGAGCGAGTGCTCGAGTATACGGAACAGCCGTCGGA GGCACTTCTGGAGACGCCGGAGAAATTCAAGCCAAAGAGCGAGTGGCCCAGCAAGGGACGCATCGAGTTCATAAACTTCA AGTTGCGTTACTCACCCAAAGAAGCACCCGTGTTAAAGGATCTTAACTTCACTATAGAACCGCGCGAAAAAATAGGAATT GTGGGGCGCACTGGCGCCGGTAAATCGTCCATCATCCAGTCCATCTTCCGTTTGGCCTGCAACGAGGGTATGATCCGCAT TGATGACGTGGACATTGAGCATATAGGCTTGCACGATCTGCGCAGTCAGGTTTCAATCATACCCCAAGATCCGGTTTTGT TTTCCGGCACTTTGCGCTACAACTTGGATCCCATGGACGAGCGTTCGGACGAGGAGATGTGGAAGGCTCTGGGCGATGTG GAGTTGCGTTCCTACGTGTCAACTTTAATTGGTGGACTGAACTGCCGTATGTACGACGGGGGTTCCAACTTTAGCGTGGG CCAGAGGCAGTTAGTGTGCCTGGCGAGAGCCATCCTAAGGCACAATAAAATACTGATTATGGACGAGGCTACAGCCAACG TTGATCCAGAAACCGACAAGCTTATCCAACAGACCATTCGCTCCAAGTTTGCGCACTGCACTGTATTGACTATAGCCCAT CGATTACACACCGTGATGGACAGTGACCGTGTGCTAGTCATGGATGCCGGCGAGGTACGCGAGCTGGGGCATCCGTACGA GCTGCTGCAGCGCACTGGAGGATACCTTCGCCAGCTGGTGGACAACACGGGCGCAGCCACCTCCTTTGCCCTGCAACAGG CTGCGGAGCAAAGTTACAGCAAGCAGATCCTGGGAGATGATACGGCCGCCGAGGACTTGAACATCACCCTTGCGATGGAG GAGAAGACCCAGTGACCGAGACGCACACTCATCTACTTGTAGATTTGCTTAAGGTTTAGATCATAAGAACTGAAAGTATT ATGTACATAGCAAAGTTGTACTTAAAGCTAACGAAATACCTATTGCCTTGTACTTACGGAATATTTGTAAGCTTGATTAT CGCCACGAATTTATGTATGTATATGTTCCATAGCGTACTGAGTAATGTATATGTATCACCTATGTTTTGTACGTCATGTG TTCAATTGGCAAACCTATCGAAAGCAATTCGCAAACGCCGTGCACAAATCAGAGCAAGTTCAATTAAACGAAGACAAATA GTGATTTAGGTACTTTTTTAAAATATGTAGCTAATGTAGGATTACCACATCGTACTTATAGATACGTACAATCTCAATAA AGTGCCTGAGAATTATTACTTACAAATGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.