Comment: 0-22 hr AEL
CTGCAGCACATCTTCAGCGCATAGATGCAGCGAAAAGCGGATCGTCTCGCGTTCGAATTTGCACGCACTGCTGCATAAAT ATTAAAAAAGCACTGGGAACATGCAGTTGCACAAACGGAGAGCCAAGCAAAAATATGGCCAGTGCATTATGTCATAAATG TGAAAGTGTTACAAGTTGCACTGCAGCGGCAGCCAGAACAACAATAACAACAACAGCAGCAGTAGCAGCAATAGCAACAT AAACTGCACGCATGTGAAAAGGAGTGCGAGTGTGTAGAAAAAATCAATTGAGAGGAAAATTGCATATTCGCTGTCGCGGC CGAAACGAAAATAAGTGAAAGAAAATAATATATATATTTATATATATAAAGTGCGTTCGGGGTGTGTGCGTGCTTGCGAG TGTGCCTGTGTGTGTGTAGGAAAGGAGCAAGAAGCAGCAGCAGCGGCAGCAGAAGAAATAGCAAAAGGAGGCAGCAACAA CAACAAGCTAGAGAAACCGCCAGCAGCAGCCCCCTAATAAAGAGCAGAGAAAAAAATGAGTTCAAGTTGTGAAAGAATAG AATGGGTAGAAATCATCGAGCCTCGCACCAAGGAGCACATGTACGCCAATCTCACCACGGGCGAGTGTGTTTGGGATCCA CCAGAAGATGTGCCAATTAAGCGGACCGACTCATCGCAGTGGTGGGAACTGTTTGATACGAATACCCAGCGGTTCTACTA CTACAATGCGGCCACGCAGAAGACCGTGTGGCACCGGCCGAGTAAGTGCGACATCATTCCGCTGGCGAAGCTGCAGACGC TCAAGCAGAACACCGATCCCAGCGAGCGTCGCGAGCAGACAACGCCCCAAAAGCAGCAGCAGCAGCAGGTGCAGCACGCT TCCCAGCAGCAGCAACATCCCTCGCCCATGTCGGGACCGAACAAGAAAGGACGTGGCCAGCAGAGGATCAGCGGTGGCAA CGGTACGGTGGCGAGCTCGGAGGAGAAGCTAACCAGCAGCGAAGTGATCTCTAGTCCACGTGGTCGTCAAAGCTTTCGCG TTAGCACTGGTGACTCATCCCGGTCGATGGACATGCAGCAGCAGCATCCGCAGGGCTATGCCTCCAGGCGTTCGCAAGAC TCCTGCAATCAGTATAAGGAATCGGGCAAGTCAAGCGACTCTAGTCTGTCCAGTGCGCATGGCTATAGGCGCTTCAATGA CAGCGGCGTTCCGGTGTCAGGACATCCGGATAACCTGCGTCTGGGTTCGCTGCAAAAGCAGCAGCGTGGCGGTGGCAAGG ACAATGGAGCTTTCGAGATGCTGCAAGAGAGCACCAGTTCGCATAATTTACCGCATGGCAGCACCTCCACATCCTCATCC TACGCCGGTGGCAGTGGCTATGGGGACAAGTATTTTGATCATCAGCTGCTGCCCAACCACCTGCAGGGCTATTCCTCCAA GTCGTCGGACATTGCCTCGCCAACACACTCCATTCACACGCCCCAGTCGCAGAAGAAGAAGATGTCGCCGGATGCAGCGC CCGCTGCACAATACAACTACGCACAACAGCAGCATCAGCAGAGAAGCGCACAACGTAGTGGCGGAGGAGCTGCTGGCACC GGAGCCCCAGCGGGAAAGTCCTACGGAGGCGATCGCGAATACAAAGTATCGCTGGCCCGCTCCGGCAGTTTCATGTCCTC TTCAATCAATCCCGCCGCCGCTGGCCACCACAGCAAATCCTACCGCAAGAGCAGCGCAGAAGCAGCGAATGGAGGAGCTG CCAGCGATGACTCAATGCACGAAAAGTACTTCAAGTCAGTGGAAAACACACCGCTCTCGCGTCGCCGCCACACCACCAAT GCAACCAAGAGCGGAGGTGGAGCCAGTGGCAGCAATACGGCCACCAGTACGGCGGGCAGCGGCAGCAGCAGCCACAAAAA GCACTCGAGCGACTCTAGTCCGCAGAGTCCGATCAGTCCACAGACAAAAAGTTCGCGGCAGGCTAAGACGAGTGCCACCA GTACGGTGCCACAGCTGCAGCATTCACCGCGCCTGCAGGAGCCGAGTCACAGTCTAGATTTGCTGAGTTTGGAAAGGATT TCGCTGGGAAAATCGGGTCCTATATCACCGGGAGGTGGGACAGAGAATCTTGGCCTGCTGACGCACTCGTCGCGCGGCTC CAACGACTCTGCCCGCCAGCGCCAGAAGTCAAATAACTCCTCGGCGAACCGTTCGCAGTCGCATCATTATCCCGGACAGA GCTCCTTGCGACATAGCGCCAGTTCTAGTCTGGACAAACGAGGCTACCATGTGGGCGGAGGGAGTGGCGATGGAAGGGGA GTAACAGGCGGTGAGAAGCGGCGCTCAAAGCAGCATCATTATCAACAGCAGGCTGACAACAGCGACGTGGAGTACGACAA TGGGAACACCTCGCCCTTGTACACCAATTGGGACCAGGAAATGCCCCACCTGCTGCCTCTCAAACACTACATCATCGAGC AGGCAAAGTTGTCCGGTCGCTATGAATACCGTGGTGGCGATGGCGGAGATTCGGACTCGTACCACTCGGATAGCCAGTCG GAACACTCACTCTCAGGACACGAACCGGACAACGAGGACTCGGACGGCGGATCGGATACGCATGGCGGTTATCTGGAGCA CAACTACGGCATGATCGACGACTACGCCAATATGGGTGATAGCGTATCCTACTACGCGTACATGTATCCGCCGCACGATC CCGCCGGAAGGGAAGATATCTCTGAAAACGTGGAGGCTGTGCTGGAGGGAATTGGCGGCAATGCGACAGAACCTTCGGCT CCAACCGCCACCGCCCCCAAGCCAAAGCCTCGTTACCAGATCTCATACTACGACACCGTATCGCACAGTCCCTCTGCCGG AAGCGGCTATCACGGTCAGCAGCTTTACTCCAATACTCCGCCCTACTCTGGGGCACGGTCATGGACATCACGTTCCACCG CCGTCACTGCCCCACCAGCAGCAGTTGCACCTGGAACCGAGCGACACTAAGCAGAAGCAGTCGCAGACCCTGCCCTCCCC TAATCGGCAGATTTCACGTCTAGCTGGCGCCGGTCATCAGGGATCGGGACCAGCTGCATCATCCAAAGAAGAGACCGGCC ACAGTACCGTTGCCGGTCAACAGCAGGTTACCGTAGGCACTGTAACGCCAGCCAGTCTTTCCCGACCTGGTCACAAGATG GCTCCGCCCTCGTTGAAGCCCACCATCCAGCAAATCTTTCCACCCAGCGAACGGGCGCCAGGAATGGGCAACTTGGGATT GGGTGGACCTACGTTGGCCTGCATGAAAGAATGTGATATAGAGAAATTCGCTGCCGACAATCTGAATTTGCACTCAAAGG GAATCTTCCGGAAAAAGGCATCTGTTCGCGACATGCTTAGTTGGACGGCGGAGGCGATTAGCAGACCCATGTTGGCATTG TCCCGGGACAAGGCGGACAAGAAGACGGCCATTGAACTGTTTAAGCTGGTGCAGATCTACATGGCGGATCGCAAGGCGCG CATAGGCATGACTCTGAATTCAGTGGCCATAGAAATTATCAGTGCATCCCTACCACAGCAGCAATTAAGAGACGAGCTAT ATGTCCAGTTGTGTCGGCAAACGACAGAGAATCCCAAGAGAGACTCGCTCATCCGCGGTTGGGAGCTGATGGCCATCTGT CTGTCATACGTACCCCCTTCGCCCACGTTTCAACCGACGCTGCTCAATTATGTCAATCGGCACCGCGATCCATCGTTTGC CACGTGCTTCATGGAGGTTAGCAAGTGGCCCATCCATGTGCAAATCTCGCACTACGCAACTGTGTGCTGTCGCCGCTTGG ATCGCATAGGTAGCAGTGGACGCCGAATGGCCAAAAAGCCAACTGTAGATGAGGTGGAGCAAGCGCGGCAACAAATCTTA AGGAACAGCATGTTTGGTAACACGCTTTCTGAAGTCATGGATCTCCAAAAAGAGAAATTTCCTTTCCGTAAGCTGCCCTG GATACAGACAACACTATCAGAGCATGTACTCCTGCTGAATGGAAAGCAGACTGAAGGAATCTTTCGAGTCTCCGCGGATG TGGATGAAGTAGGTTGCATGAAGAACCGCCTGGATCGGTGGGATGTTCCTGATTACAAAAACACGTTGGTTGATGCACAC ACGCCAGCCAGTCTGCTTAAGCTGTGGTACCGAGAACTGTACGATCCACTTATACCGGATGCCTACTACGAAGATTGCGT AAACACAGAGGATCCCGACAAAGCCAAAGAAATAGTTAATAAGTTGCCTGAAATAAATCAGCTGGTTCTAACGTATCTAA TACATTTCCTACAACAATTCGCCATACCCGAGGTGGTGAGTTGCACCAAAATGGACTCCTCAAATCTGGCCATGGTGTTT GCGCCCAACTGCCTGCGCTGTACATCGGAGGATCCGAAAGTGATCCTGGAGAACGCTCGCAAAGAGATGTCCTTCATGCG CACCTTAATTCAGCACATGGACACAACGCACGTGGCCAATCTGGTCTAATGGTCTGTTAGCCATCCAAAAGGGCAAACGA CCTGCCTACCAATCTACCAATATGCAAATCGTTATGAACCTATCCTAACTTACTACCTACCAAATCTAACTTTAACTCAA TTTGTATTTATACAAAGGATGATGATGAAGATTAATCCTAACTCCTACTTTAACTTTAACTCGTTTGCTACTCGAACTTT CTCACTAATTGCTATCAGGACGAAAAGTCGATGGATTTAATTTTATTTCGTAATGACTAGGAAGCTTGTTCAGAACAATG GCAACAACCCCAAATACTCGACGATCTTGTAACCCGTAGTGCTTTTTCAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
