Comment: 0-22 hr AEL
CTGTCAAGAGCACAAAAAATAAGTGAAGAGCAAGAACGGAAAAAGCAAAGCGATGCGAATGCAGCGGACAAAGCGAATGT GACAACTACAACACGACGACGAAGGCAGAAAAGTGTATTTTAAGCAGCGGTTGGCCAGCCACAGAGATTTTCGTGGAAAT GCCCAAGAGTAGCGCAAGGACGTCATGAATGCGTTCCAGGACTTTGAGCTGGGGGCCAAACTATATCTGCAGTGCTTATT ATCGCTGAGCAGCTCCCGATCTGCCACGCCATCCTACACCTCACCTGTTAACCACGCTGGAGCTTCTCCTCTGAATGCCA TTGCCCATTCGCCGGTGAATGTGTCCGCCACTCATAGGCAGAACTTTTTCACACCCATTGCCAACCAGTCGCAGCAGCAA CAGCAGCAACAGCCTGTGGCGGTACCGCTCGACTCCAAGTGGAAAACAACACCCAGTCCGGTGCTCTACAATGCCAACAA CAACAGCAGCAATAACAACACCAGCAGCAGCAACAACAACAACAACAGTAACTGGGAGGTGGGCAGCAATAGCAATACTC ATGTTGCAGCTACGGCAGCAGCAACATCAACAGTTGGCGCTCAACCACTGCCGCCGCAAACGACACCCGTTTCGCTGGTG ATGCACGCCCCACCGCCGCAGCAGCAACCACTGCAGCAGCAGCACCACCATCACCAACCGCCTCCACCGCCACCTGCTAG TTTGCCAGCGCCATCGGCACCACCCACCAGCGGCAGCAGCAGCAGCCACAACAGCGTGGGCCATGCCACCAGCGTTATAC GCATTTCGAGCAGCCAACAGCAACATCAGCAGCAGCAACAGCACCAACAGCAGGCACATCCACATGTGGTGGTGAGCGGC GGACAGACTTTTCATCCCGTAATCGTGGACGCTACTCAGCTATCGGTGCCGCTGCCACCCACAACGGTTTCATTTCATCA GCCCAACACGCCCACCTCAACAGCGGCATCAGTGGCTTCAATGAGCCAGGACAAGATGCTGGCAAAAAACGGCTACAATG CGCCCTGGTTCAAGCTGTTGCCGCATATGACGCCAATGAGCAAGGCGTCGCCAGCTCCGGTCACTCCGACCCTAACCACA TCGGCCAGCAGTTACAACGTGGTGATGATGCAACAGCAGCAGCAGCATCAGCAGCTTCAACAACAGCAGCAACTGCAGCA GCAGCAACAGTCGCCACCCCAGATGCCGCTGAACCACAACAACAATCATCTGATTGTGTCCGCCCCACTAAGCTCCCCGG GCAAGCCACTCAACTGCTCAATGAACGATGCCAAGGTGGCGGCGGCAGCAGCAGCGGCCGCAGTTGCCAACCAGAGGCAA AAGCAGCAGCAGGAGGAGCCGGACGATCAGCTGGACGATGATGTGTTTGAGACCACAACGCCCGGTATCAGCGCCAACAG CAAAAAAACAAACTGCGGCAATGCGGCTGCCCACCCACAACAGTAACATACGAAAGCTGGAGGAATGCCATGATGATGGA GCTGCAGGTGCACCAGCCACTTCGGCTGCCAAGCGGAGGAGTCAATCGTTGAGCGCCCTGCAGCAACAGCAGCAGCAGCA ACAGCAGGCCGGTGCGGCAGGAACAGCAGCTGGGCAGCCGGCGAACAAGAAAATCCGAAGACCCATGAACGCTTTTATGA TCTTCTCAAAGAAACATCGGAAAATGGTGCACAAGAAGCATCCGAATCAGGACAACCGTACGGTCAGCAAGATTCTGGGC GAGTGGTGGTACGCCCTGAAGCCAGAGCAAAAGGCTCAGTACCACGAGCTGGCCAGCTCTGTTAAGGATGCACACTTCAA GCTGCATCCGGAGTGGAAGTGGTGCTCCAAAGATCGCCGCAAGTCATCCACCTCGACAGCAACGCCTGGTGGAAAGGCGA GCGGAGCGGCCGGAACAGGTGACGCTAAGCAGCGCCTGGTATCGGTGGATGGCTCCGATTCCTTGGAGCACGATATGTGC CCCTCAACGCCAGGAGGCAGCGGCAGCTGCGGTGGCCAGGGCATATCATCCGATTTGCAGGGCGATATTATACCGCTGAC AATTGACAACTACAATAGCACCTGTGATGAGGCGCCAACTACGATCTCGATGAAGGGCAATGGCAACGGAAAGCTGATGA AGAACGAGCTGCCATCGGACGAGGACGAACAGATGCTGGTAGTGGAGGAGGAGCAGCAACAGCAGACCGTAAAGAAGATT GACCTGCACTGCCGCGAGCGGGTGAATGACTCGGACATGGACGACACACCCTTTGACTACCGCAAGCAGCAGCCAGAGGC AAATCAGCGTTCGGCAGAGGAGCACAGTACATCCGGTGCGAATGGCCAGGCCATAAACGCACCGCCGCTCAGCGGAGGAG AGCGCGAGATCACACTCAAACCGAAGGCTATCAAAGCACATCCGGTGCTGGAGAGCAACATGCTGCCATATACCCAGATG TCCATCTACACGCAGTACACTAGTCCCAAGAACCCAATCGGTGTAACACCATTCCAACCCACAGGCGGCGCCTTCAAATC GATGCCCATCAGCCCCAAGGGCAGCGGTGGCAAGCCGGAGGACGCTGGCTCCCTGCAGGCACACATCAAGCAGGAGGACA TCAAACAGGAACCGCCATCGCCATACAAGCTGAACAATGGTTCGGGATCCGCCTCCGGAGGGGGCGTGGTCAGTGCTCCG CCGCCGAATAGTGGTAGCGTTGGTGCCATATTCAACTTCAATGTGCCAACGGCGACGGCTTTGAGTCAGAAGCAGTTTCA CTACCCGATGCATCATCCTCATCGCAGTCCCACAGATCTTAGAGCTGCCCACCAGGCGTGTGTGCCATCGTCGCCAGCGG GCATGGGACTGGGGCATGCGGCCAACATTGCCACGCCTCCAGCATCGGCGCCGGCCCAGATCATGGGAGGCGGTCCAGCC AGTCAGAAGATGTTCTTCGCCATGACTCATCCGTATACGTTGCTGCAGCGCTCCCACCAACCGGGCACCCCCAGCCTAGA GCACTTGCAGCTGGACGCTTTTGCGCCAGGAGGTTACACCTTGAGGAACCACAATGGCCTGTCTTCCCTGCCACCGCCCG TGAGTGCGCAACCCACGATGCTGCTGCACGGATACCCGCCCAGCCATGGTGTCGAACCACCGGCTAGATCGCCATCGTAC AAATCAATGCCATCTACTCCAAAGTCGGCCACATATCTCATGAGTGCGCCACCGGAGCGAGGCATGGATGGAGGAATGAG TGGATGTGCATCAGCAGCGGCAAGCGGAGGCGATGAAAGCGACATAGATGCGGATGGCCAGCAGTTTATTTTGGCACCCA CGCCTGCGCAGTTGGGTCGAGCTCCACTGCAGAGGAGGAAGAATCTATCTCAAAGCAAGTCAGAGAGCAATGTGTCCTTT GGCGCTAATCTGGGGGCCAGCAATGGACAGCACATTAGTCGCAAGCTGCACTCACCCACAATGATGGAATCCTCATCGCC CATCATAGGCCACGTGAACAGCAGCAACCTCAGCTCGGCCCTGCCAACGCCCACGTCCTCGACGACGACACCAAACAGTG ATGAGCAACTGCCACTGACGCCGACAACCAGCAGCAGCAACAGTAACTTAAATCAGCAGCAGCCCAAGTCACCGATGAAA GGTGCTCCAGGATCAACGGCAGCGGCCCTAAAAAAGAAGAACGATGAGATGAATAACAGTGTGCTCAAGCAGGTAGATTT CGAAAAGAAATACAAGGCCCTGCCGCAGTTCCAGCCTGAGGATTGCCAGTCGCCCAGTGCCATTGCTGTGCCCTCATCGC CACGCGTTTACGGCACGAATTACCGCAAGAAGAACACGGCTCCACCGCCAGTTCAGAAGCTAATGTGCGAGGACGACTCC ATTGACGAACCAGCATCGGCACCGCCTACGACCACACAGCGATTCTTTGGTCCGGACTTTAACAATGAGCTAAAGGAACT GGAGAGCTCTGACCAAACGGGCCGCTCACCCAGGACGCCAAAGACACCGCTACAAAGCGCTCGGTCCGATGCCAGTGAGA AAGGACACCGCAAGGTCCTGGAGACGCGTCGCAGCCTGGTCTTGCAGTTGTTTGCCGAGCACGGTAACTTCCCCACTGCC CAGGCCACTATGGCCTTTCAGTCTAAGCACAGCGATGTCTTTCCGCGCAAGCAAGATCTGCAGCTGAAGATACGAGAGGT GCGCCAGAAGCTGCTGGGCCAAGCATCTTGCACTCCACACTCGGCGGGCCCCAACACGCCGTCCGACTCCAACTCGTCGT CGACCACATTGAGTGCCAGTAGCACCAGCTTGAATATGCAGACCACCAGTGCGGCAGATGTTTTTCAATATTACTGAACA ACAGGTGGCCACCAGCAACAGCACTCTGAGCAGCAGCCCCATTCCGCCACCAAAGCCACTGAATCTGATGCCAAGTACAA GTAATGGGGCGTGGACAAGGACGAGGAGTTCGGCGGGGACAGGACAGGACAATGGATTGTTTGCTGCAAGTGACAAAATT ACTTAAGGATAGTAAACGAATCGACAACAACAATTGCAGCAACGCAGACTCACAAAACGAAACGGATGTGCAACAAAGCT CACACTTAGCAAACGCTCATGCATCAGATGCATGGTTAACTAGTTTTTATTATTAACTGCTAATAGCGAGGAAGCAAAAC ATACAGAAAATAGAGAAAATTACAAATTTTTAATTACAATAGGCATACGAAATATGTACAAGCAACGTAATTTGTGAGTC GAGTGGAGAAACTGATTACAAATGAATTTATTAAGCAGCTTAATAAATCGGGAGAAGTAGAAATTTTGATGAAAATTTGA ACTTTTGAAGCACATATGAAGTACATACATACATAAATAGAAAACATACATAATCGAAACATTGTATAATCCCCGCGTAA AGGCTTAGGTTTTAATTTACAGGCAAAACTGCAACCAACAAATTGGAAGAGCAAAGAAAGAACTACGGAAATAGAAGTTA AGCTTTAACTTAAATTGCACATTAATTTTGAAGTGTATCAAGCACAAGCAAAGAATTGTATAAATCAAACAGTAAGAAAC TTGAAATAAACACTGTAGTTGTATTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
