Comment: 0-22 hr AEL
CGTTAACGGTTGCCTGCGTCTTCTGAGCGACGAACATATGAAGTCGCTCAGGCACACCAACAAGAATAGGATTTCGTAAT GAATTGACGAGCGAGACACCAAAGTACAACCTCGAAGGAACAACCCACCAGCCCAGAGAGCCACACAGACTTTGGAATTT CTCCACTCGTCCTAGTGACACCCAAGCCCACCTGTAGCCCAACGAGCCACCCAAAACCGAACCCACCATGAAGCGCAGCC GGTGGAGCCACAGTGGCTCCTCCACGGCTCGACTCCTGCTGATCGGAGTATTGTTCGCCAGCTGTTCCACCGCCATCCTG GGTGCCCAGCGTCCCCCCATCAACTCCGCCGGAGGTCACGAACTGCGCGGAACCACCTTCATGCCGGCCCTGGAGTGCTA CGATCCATACGGCAGGCCACAGAAATGTCTGCCAGAATTTATCAATGCTGCCTATCAACTGCAAATTGAGTCAACTAATA CCTGTGGTGAGCAGAATGACAACCACTTCTGCATACAAACCATGAACCAAAATCACAAAAACTGCGAATTTTGCAAATAC AATGATCATAATCCATCCTTCTTGACGGATTTGCATGATCCGCAAAGTCCAACGTGGTGGCAATCGGAGACCATGTTCGA GGGCATTCAGCACCCGAACTATGTGAATCTGACTTTGCACCTTGGAAAATCCTATGACATCACCTACGTGCGCATTCTCT TCCGCTCACCAAGACCCGAATCCTTTACGATTTACAAGAGGACCTCGGAGAGTGGACCCTGGATTCCCTACCAGTTCTAC AGTGCCACCTGTCGCGACACCTACTCCCTGCCAGATTCGCGAGCCATTCGCAAGGGTGAGGGCGAGGCCCATGCTCTGTG TACCAGCGAATACAGTGATATCTCGCCGTTGAGGGACGGTGAGATTGCCTTCTCCACGCTGGAGGGTCGTCCCAGTGGCA TCAATTTTGAGCGCAGCGGAGAACTGCAGGAGTGGGTCACGGCCACGGATATCCGTATCACGCTGGACCGACTGAACACC TTCGGTGACGAACTCTTCGGTGATTCCCAGGTGCTCAAGTCGTACTTCTATGCCATCAGTGACATTGCCGTGGGTGCGCG TTGCAAGTGCAATGGACATGCCAGCAAGTGTGTACCGAGCACGGGAATGCATGGCGAGAGGACTCTGGTCTGCGAGTGCC GGCACAATACGGATGGACCCGATTGCGATCGCTGTCTGCCACTCTACAACGACCTCAAGTGGAAGAGATCCACCTCAACG GAGGTCAACGAGTGCAAAGCTTGCAACTGCAATGGATTGGCGGACAAGTGCTTCTTCGACGCGAATCTGTTCAATCGGAC GGGTCATGGAGGTCACTGCCTGGACTGCCGCGAGAATCGCGATGGACCCAACTGCGAACGCTGCAAGGAGAACTTCTATA TGCGCGACGATGGCTACTGCGTCAACTGCGCCTGCGATCCCGTTGGCTCCAGATCGCTGCAGTGCAACAGCCACGGCAAG TGCCAGTGTAAGCCGGGTGTGACTGGCGACAAGTGCGACCGCTGCGATAACAACTACTACCAATTCGGTCCCCATGGATG CCAGCAGTGCGGATGCGACAGTGGGGGATCCCATCAGAATACACCCGCCTGCGATACCGAGACTGGAATCTGCTTCTGCA AGGAGAATGTGGAGGGCAGACGCTGCAATGAATGCAAGCCAGGCTTCTTCAATCTGGACAAGAACAATCGATTTGGTTGC ACACCCTGCTTCTGCTATGGGCACACCTCCGAGTGCATGACTGCCCCAGGATACTCCATTGTTTCGGTCACCTCCAATTT TAACAAATTCAAGGAACGCTGGACGGCCGCCGATTTGAATCAACGCGAGGTGGACATCAAGTACAACCAGTATAGCCGAA GCATTGGAACCACCGCTCAGGGAAATGAGCACGTCTACTTCCAGGCACCGGATCGCTTCCTCGGCGATCAGCGTGCCTCC TACAACAGGGATCTGAAATTCAAGCTCCAGCTAGTTGGTCAGGTGGCCAATACCGGAGTGAGTGACGTGATCTTGGAGGG TGCTGGCAGCCGCATCTCCCTGCCCATCTTCGCCCAGGGCAATGGAATACCCGACCAGGGAGTTAAGGAGTATACCTTCC GTCTGCATGAACATCATGATTACCAGTGGCAACCAAGCCAGTCGGCTCGTGGATTCCTTTCGATTCTGTCCAATCTGACG GCCATTAAGATCAGGGCCACGTACTCGGTGCAGGGTGAGGCCATCCTGGATGATGTCGAGCTGCAGACGGCACATCGTGG AGCCGCCGGCCACCCGGCCACCTGGATCGAGCAGTGTACCTGTCCGGAGGGTTACCTGGGCCAGTTCTGTGAGTCCTGTG CTCCAGGCTATCGCCACAGTCCCGCTCGCGGCGGTCCCTTCATGCCCTGCATCCCCTGCGATTGCCATGGTCATGCGGAC ATCTGTGACTCGGAGACGGGCAGGTGCATTTGCCAGCACAACACCCACGGCGATAACTGCGATCAGTGTGCCAAGGGATT CTACGGAAATGCCCTGGGCGGAACTCCCAACGACTGCAAGCGTTGCCCCTGTCCCAATGATGGTGCCTGCCTGCAGATCA ACGAAGATACGGTCATCTGTACGGAGTGCCCGAAGGGTTACTTTGGTTCCCGTTGCGAGCAGTGTAGCGATGGCTTCTTC GGAGATCCCACGGGTCTCCTGGGTGAGGTGCAGACTTGTAAGAGCTGTGACTGCAATGGCAACGTGGATCCCAATGCCGT GGGCAATTGCAACCGGACTACGGGCGAGTGCTTAAAGTGTATCCACAATACAGCCGGAGAGCACTGTGATCAGTGTTTGT CCGGACACTTTGGTGATCCTCTGGCCTTGCCTCATGGACGCTGTGATCGCTGCAGTTGCTACGAGGCTGGAACCGAGCAG GATGAACAGAGTATCACGCGATGTGACCAAGTCACTGGTCAGTGCCAGTGCAAGCCGAATGTAATTGGAAGGGATTGCGG AGAGTGCCAGCCGGGCTATTTCAACATCCGATCGGGCAATGGCTGCGAGAACTGCCTGTGCGATCCGGTGGGCAGCTACA ACTCCACCTGCGATCGCTACTCTGGCCAGTGTCACTGCCGACCAGGTGTAATGGGTCAGCGGTGCGATCAGTGCGAGAAC TATTTCTACGGATTCTCCAGCGAGGGCTGCAAGCCATGCGAATGCGACGAGAGCGGCTCAAAGGGATTCCAGTGCGACCA GAATGGTCAGTGTCCTTGCAATGATAACGTGGAAGGACGTCGTTGCGATCGCTGCAAGGAGAACAAGTACGACAGGCATC GGGGTTGCATCGATTGCCCCGATTGCTATAACCTCGTCCAAGATGCCGCCGATTTGCATCGTGCCAAGTTGTTCAATCTC AGTCAAACGCTGGACGAGATTGCTCGCACGCCGGTGACCAATGACGATGAGTTCGAGGCCAAGTTGAAGGCGGTGCAGGA GAAGGTGGCTGTCCTGGCCCAGGATGCACGCGATAATTCCGGTGATGGTGGTCAAACATACGCAGAGGTCATCGATGATC TTCACAAGCACCTGGACAGCGTGAGAGAGCATCTGGTGAGCGCGGATAAGTTCCAGGCAGATGCCAATGGGGAGATCGAC AGGGCGCGCCAGAACTATACCATTCTGGACCAGATCACCGAGAACGCCAAGAAGGAACTGCAGCAGGCCCTCGACCTCCT GAATGACGAAGGTGCTCAGGCTTTGGCCCGGGCCAAGGAGAAGTCTGTGGAATTTGGACAGCAATCGGAACAGATCTCGG ACATCTCGCGGGAGGCTCGTGCCCTGGCCGACAAACTGGAGTCGGAGGCCCAGTTCGATTTGAAGAACGCCAAGGATGCC AAGGATGCGGTGGAGAAGGCCCATCAGCTGGCCAAGAGTGCCATCGATTTGCAGCTAAAGATCGGCACCGAGCTCCGTTC GGAGGTTGGTCTGGAGCTGAGTCATGTGAAACAATCCCTGGGCACCGTAGTGCAGACCTCCAAGGAGGCTCTTCGCAAAG CCAACGAGGTCTACGATACAGCATTGACCCTGCTCAATGATGTCAATCGTCAGACGCAACCGGAAATCGATATCAGTCAG TTGAAGAAGGATGCAGTGGCCGCCAATGAACGAGCGGATGAGCTACTCAAGCAGATAACTGAATTGTCCAACAGCAACGG CGAACTCTTTGCGGATTTCGAAACGGAACAGGAACTGACGGAGGCGCTGCTTAAGAGAGCTGAACAGCAGCAACTGGAGG ACATCGAGCTGCTAGAGCGAGCCAAGGCTGCCCACGACAAGGCTACCAAGGCAGTGGAACAGGGCGACAATACCCTAAAG GAGGCCAACAATACCTACGAGAAGTTGGCCGGCTTCCAGTCGGATGTCCAGCGCTCTTCTGAGAGTGCGGAGAAAGCCCT GCAGACGGTGCCCAACATCGAGAAGGAGATTCAGAATGCGGAAAGCCTGATCAGCCAGGCGGAGGAGGCTCTCGATGGAG CCAATAAGAATGCCAACGAGGCCAAGAAGAATGCCCAGGAGGCGCAGCTGAAGTATGCCGAACAGGCTTCCAAGGATGCT GAGCTGATCCGCCGCAAAGCCAATGAGACCAAGGTGGCTGCCCGCAATCTGCGCGAAGAGGCCGACCAACTGAATCATCG CGTGAAACTCACCGAAATGGACATCTTCAAGCTGGAGGAGAGCTCGACCAAGGACGACAATCTGGTGGACGACGCCAAGC GAAAGGTGGGTCAGGCCAAGGCCGATACCCAGGAGGCCCAGAAGCAGATCGAGAAGGCCAATGCCGACCTGACGGCCATC AAGGATGAGCTGGAGAATCTGAAGGACATCAACACGGGCGATTTGGATAGATTGGAGAATCGTTTGGCCACTGTGGAGGG TGAGATCAATCGCGTCAACCTGACGGGACGCATCGAAAAGTACCGGGAACAGCGCACCATTCAGAAGAATCTGATCGACA AGTACGATGCCGAGCTGAGGGAGCTCAAGGATGAAGTCCAGAATATTGGACTTATTTCCAAGGCTCTCCCCGATAGCTGC TTCAGTCGCAATCGCCTGGAACCCTAGAGCTCTGCGAACAACCCCACTTCCCATAACAACTACTTCAATTTTAACTAAGA ACGAAGATCTGAGACGAGACGAACAAATCAGATTTATGATAGAACGTAGACGTTGAATGAAATTATGTAATTTTAGTGCC TTAGCCGAAATATTCTGTTTAGTGACCCAGCCCAAGGATCCAAGGATCCACAAAGGAGACCAACTGAGCAACGTTAAGCT TTAACGCATCATCGTTCGCCTACTGTATGCCATATACTATAATGATACAACGGATACAAGAGGATGGATGATGGCCACAT CGAGAATCAACTGCCAACTGTGCTTCACCAGTTTCAAGCCAGCAACGGACTGTATTATATGTTAAAAATGAGACACTAAA CGAAACACACAAGACCTTGTACTCCCACAATCCGACAATCCAATCGCATATCTATATCTCTACCGTAGCTCCACCTGAAG TCTATTCTAAACACTTATATATTAACATATTTGTCATCTACGTTTAATGTGCCAATACAATTACCATGTATCTTTGTGTA TAAGTCGCTTAAAGAGTTTCCTTTCAAAATATAACTTTTCCAACGTTGTAAGTTTGTAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.