Comment: 0-22 hr AEL
ATTCCAACGCAACGCCAACTAAGTGCAAAAAAAATCTAAAATTTGTGTAAAATTGTAAAATCCCAATCGCGTATATTTCT AAATCGTATTCGCACAGCACACAAAAATATAAAATAAAAGCAAATTAAGCGAATTATACATATATTTGTTAAGTTTTTTA AATCGTTTACTTTTAATACGCGTGTGTCCTAATAAGTGAAGATTATTAAGAATAAAGTAAAAAATGAGTGATGATACGAG CGCTTCGGGCGGGACAAGTGCCCCTTTTCCATCGCCAGTTACTGCGGACCCGGAACCAGGAGCCACTGCCTCCAAATTAC CGGGTCCCATCAGATCCAATATTCCCACGCCCGCTACTTCTGGTACCGGAATCCCGCAGCCCAGCAAAATGAAGGCACCA TCTAGTTTTGGATCGACGGGTTCTGTTTCCAAAATCGGAAGACCGTGCTGCAATCACACAACTCCCAAATCCGGTCCACC ACCAAGAGAAGCCACCAGCATGAGTCGTGAAAGCGATGACAATTTAAGTTCGATCAATTCGGCTTATACAGATAACAGCA GCGCCGTGCTGACAGCAAACACAGAGCAGTTCATCATTGGACAGCGGGTTTGGCTGGGTGGCACTCGTCCCGGACAGATT GCCTTCATTGGAGACACACACTTTGCTGCTGGCGAATGGGCGGGTGTTGTCCTTGACGAGCCTAATGGTAAAAACGATGG CTGTGTGTCGGGCAAAAGGTACTTCCAGTGCGAGCCGAAACGAGGCATTTTCTCACGCCTCACACGTCTTACCACATATC CCTTGGCCGGAGCCCAGACTCCGACCTCTCCATTGGCCAAAAGCTCCCCAGACAGATCGCGCACAGTTTCTCCAACTGCG AGTATTCGCAGCTCTATGCTTCGCAGTCCCGGCATTGGAGGCAAGAATGGAATGGCTGTGGGCGATCGTGTGATTGTCTC TTCTGGATTTGGTAGTCGTCCTGGTATCTTACGCTATTTGGGAGAGACACAGTTTGCTCCCGGCAACTGGTGCGGTGTGG AATTGGATGAGCCTAGCGGCAAAAACGATGGAACTGTCGATGATATCAGATACTTTGAGTGCAAGCCCAAGTACGGGGTG TTTGTACCTATTGCGAAGGTTTCACTGTCGCCGTCGTCCAAGAAAACGCGTCTTTCCAGGACCGGATCAAGGGAGTCGCT CACCTCGATTGGCACCATGAACAGCATCGCCACCACGGCCACGTCGCGCATGCGCATGAATGCTCAGCGCAAGTCGAGCA CGCCCGTTAAGCCAATTTTAGCGACGCCGAAAAGCCAATTTTCCATGCAGGATCTGCTGCGCGAGAAGCAACAACATGTG GAGAAGCTGATGGTGGAGCGCGACCTGGACCGCGAGGATGCCCAGAACCAGGCGCTGCAGCTGCAGAAGAACATCAACGA GCTAAAAGCAAGAATCGTTGAATTGGAGTCGGCATTGGACAATGAACGAAAGAAAACTGAAGAATTGCAGTGCTCCATAG ACGAAGCCCAGTTTTGTGGCGATGAATTGAATGCTCAGTCACAGGTTTACAAGGAAAAAATCCATGATCTGGAGTCAAAA ATCACAAAACTGGTGTCCGCCACGCCAAGCCTACAAAGTATACTACCGCCCGATCTACCTTCAGACGATGGTGCTTTGCA GGAGGAAATCGCCAAGCTGCAGGAAAAGATGACCATTCAGCAGAAGGAGGTTGAATCTCGGATTGCGGAACAGCTGGAGG AGGAGCAGCGGTTGAGGGAAAATGTAAAGTACCTTAATGAGCAAATCGCCACTCTACAGTCCGAGTTGGTGTCCAAAGAT GAGGCCCTGGAGAAATTCTCCCTCTCGGAATGTGGCATCGAGAATCTCCGAAGGGAACTCGAACTTCTCAAGGAGGAGAA CGAAAAGCAAGCTCAGGAGGCTCAGGCTGAGTTCACCCGAAAACTAGCCGAAAAATCCGTAGAGGTGTTAAGATTAAGCT CTGAATTGCAAAACTTGAAGGCAACATCCGATTCCCTGGAAAGCGAAAGGGTTAACAAAACCGACGAATGTGAAATTCTT CAAACCGAAGTACGAATGCGGGATGAGCAAATCAGAGAGCTAAACCAACAACTCGATGAGGTTACCACACAACTAAATGT ACAAAAAGCGGATAGTTCTGCTCTGGATGATATGCTTCGATTGCAAAAGGAGGGTACTGAAGAAAAATCTACTCTTTTAG AGAAAACCGAAAAGGAGCTAGTTCAAAGCAAAGAACAAGCTGCGAAAACTCTAAATGATAAGGAACAACTTGAAAAACAG ATATCAGATTTAAAGCAATTGGCGGAACAAGAAAAACTAGTCAGGGAAATGACTGAAAATGCAATCAATCAAATACAACT AGAAAAAGAATCCATAGAACAGCAATTGGCTTTAAAACAAAACGAACTTGAGGACTTCCAAAAGAAACAGTCAGAATCGG AAGTTCATCTTCAGGAAATCAAAGCTCAGAACACGCAGAAGGATTTTGAATTAGTTGAATCTGGTGAGTCCCTTAAAAAA CTGCAACAGCAATTGGAGCAGAAAACTTTGGGTCATGAAAAACTGCAAGCTGCTTTGGAAGAACTAAAGAAAGAAAAGGA AACGATCATAAAGGAAAAGGAGCAGGAGCTCCAGCAGCTCCAAAGCAAGTCAGCTGAATCCGAAAGTGCTTTAAAAGTCG TACAAGTACAACTAGAGCAACTCCAGCAACAGGCTGCCGCATCTGGAGAAGAGGGATCCAAAACTGTGGCCAAATTGCAC GATGAGATTAGTCAGCTTAAGTCCCAAGCTGAAGAAACTCAGTCCGAGTTAAAATCCACCCAATCCAACTTGGAAGCCAA AAGCAAACAATTGGAGGCAGCAAATGGCAGCCTAGAAGAGGAAGCCAAGAAGTCAGGCCATCTGCTGGAACAGATTACCA AACTTAAATCTGAAGTAGGGGAGACGCAGGCAGCTCTCAGTTCATGTCATACGGATGTGGAATCCAAAACTAAGCAACTT GAAGCCGCAAATGCAGCTCTGGAGAAGGTCAACAAGGAATACGCGGAATCCCGAGCGGAGGCTTCTGATCTGCAAGATAA GGTGAAGGAGATCACCGATACGCTACATGCTGAGCTTCAAGCTGAACGATCGTCCTCCAGTGCTCTCCATACTAAGCTGT CCAAGTTCTCGGATGAAATAGCTACCGGCCACAAGGAACTGACCAGCAAAGCCGATGCCTGGAGCCAGGAGATGCTGCAA AAAGAGAAGGAACTACAGGAGCTGCGACAGCAACTTCAAGATAGTCAAGACTCTCAAACAAAGCTGAAAGCAGAGGGAGA GCGGAAAGAAAAGTCTTTCGAGGAATCTATAAAGAATCTTCAGGAAGAAGTCACTAAGGCCAAGACGGAGAATCTAGAGC TAAGCACTGGCACACAGACGACCATAAAGGACCTGCAGGAGCGACTGGAAATCACCAATGCTGAGCTCCAGCACAAGGAA AAAATGGCAAGCGAAGATGCGCAGAAGATTGCCGACCTTAAGACCCTTGTGGAAGCCATCCAGGTGGCTAATGCCAATAT ATCAGCTACAAATGCGGAGCTCTCCACTGTATTGGAAGTTCTTCAGGCGGAGAAGAGTGAAACAAATCACATATTCGAGC TCTTTGAAATGGAAGCCGATATGAACTCAGAGCGGTTGATCGAAAAAGTTACTGGGATTAAGGAGGAACTAAAGGAAACC CATCTGCAACTGGATGAGCGACAGAAAAAGTTCGAGGAGTTGGAGGAGAAATTGAAGCAAGCTCAGCAAAGTGAACAAAA GTTGCAACAGGAGTCTCAGACTTCCAAGGAGAAACTTACGGAAATACAACAATCCTTGCAAGAACTCCAAGATTCTGTAA AGCAAAAGGAAGAACTTGTCCAGAACTTGGAAGAAAAGGTTAGGGAAAGCAGTTCCATCATAGAAGCGCAGAACACGAAA CTAAATGAAAGCAATGTTCAGTTGGAAAACAAAACTTCTTGTTTAAAGGAAACCCAAGATCAATTGCTAGAGTCACAGAA GAAGGAGAAACAATTGCAGGAAGAGGCTGCCAAACTTTCCGGTGAGCTGCAGCAAGTGCAAGAGGCCAATGGAGACATAA AGGATTCCCTAGTAAAAGTAGAGGAACTAGTAAAGGTGTTGGAGGAAAAACTCCAAGCAGCCACCTCCCAGTTGGATGCC CAACAAGCCACAAATAAGGAACTCCAGGAGTTGCTGGTTAAATCTCAAGAAAACGAGGGAAATCTGCAAGGAGAATCTTT GGCAGTCACTGAGAAACTACAACAACTGGAGCAAGCAAATGGGGAGCTTAAGGAGGCTCTGTGTCAAAAAGAGAATGGCC TTAAAGAACTTCAGGGCAAACTTGATGAAAGTAATACTGTATTAGAAAGTCAAAAGAAGAGCCACAACGAAATTCAGGAT AAGTTAGAACAGGCCCAGCAAAAGGAGAGGACTCTTCAAGAGGAAACATCCAAGTTGGCGGAGCAACTGAGCCAATTAAA GCAGGCTAATGAAGAGCTCCAGAAATCCCTTCAGCAAAAGCAGTTACTTTTGGAAAAGGGTAATGAATTCGACACCCAGC TGGCAGAATATCAGAAAGTCATTGATGAGATGGATGATGCGGCTTCCGTTAAATCCGCGCTGCTGGAACAGCTACAAAAT AGAGTTGCGGAACTGGAGACCGCACTCCGTCAAGCCAACGATGCCCAAAAGACTGCTTATCTGGAGACTAAGGAACTGAG GCGCCAGCTGGAATCGCTGGAACTGGAGAAGTCGAGGGAGGTTCTGAGCCTAAAGGCTCAGATGAATGGTGCGAGCAGTA GGTCCGGAAAGGGTGATGAAGTGGAGTCACTGGACATCGAAACCAGTCTTGCCAAGATTAACTTCCTGAACTCAATTATT GCTGACATGCAGCAGAAGAATGATGCACTCAAGGCAAAGGTGCAGACCCTTGAAACCTTGCCAATGGATTTCACCAAACC TCATGCCTTCGATGCCCTGACAAAGCGGAAACCGGCTCCCAGACTTTTCTGCGACATCTGCGATGAGTTTGATCAGCACG ATACGGAGGATTGTCCAATCCAGGGAAGCGAAGATCAAGACTACTCCACACCGTCATCCGAGTCCAACAACAACGAGAAG GAGCGGAAGCTGCCGGCACCCAGGAAATACTGTGATTCCTGCGAGGTTTTTGGCCACGATACGAGCGAATGTGCCGATGA TGAAACCTATTAGTTAGCTCTAGTTAAATTCTATTATTTCCATTTTACTTTGTTTTAGACTCTATAGCATAACCATATTG CGAGATCTATCAGATGAGATACTTTTACCTCAGCAGAGCTTCACTCAATCACACAAAGAAATTTCTCTACTTAAAATTGT GATCTTGCGTTATGCACACATCCATGGATAGTAGTCTATTTTAAATTGTTTTTATTTATTGAATGCGATTCGTGGCAAAC ACACAATCGGTTGTTTTCGTAATTTTTGAGTGTAAACATAGTGTAAAAAATAAAATCAAGAACGTGAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
